CMV启动子克隆及其在植物体内的表达

doi:10.3969/j.issn.1000-1565.2014.02.013

摘要/Abstract

摘要： 根据细胞肥大病毒CMV启动子基因序列设计引物,PCR扩增目的基因后,利用基因重组技术成功构建具有Kan抗性和GUS intron报告基因的植物表达载体LpPCG,并将重组质粒转化到烟草叶片中.通过CMV启动子指导的GUS intron基因在烟草叶片内的瞬时性表达,比较了其植物表达特性.结果表明:CMV启动子可启动GUS在植物体内的表达,其表达活性相当于2×35S启动子的(80.4±26.6)％.

关键词: 植物, CMV启动子, GUS, 瞬时表达, 增强型35S启动子

Key words: plant, CMV promoter, GUS, transient expression, enhanced 35S promoter

中图分类号:

Q945.78

于秀敏,岳文冉,王瑞刚. CMV启动子克隆及其在植物体内的表达[J]. 河北大学学报(自然科学版), 2014, 34(2): 187-192.

YU Xiumin,YUE Wenran,WANG Ruigang. Cloning and expression study of CMV promoter in plant[J]. Journal of Hebei University (Natural Science Edition), 2014, 34(2): 187-192.

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