Box-Behnken设计响应面法优化超声波辅助双水相法提取玛咖多糖

doi:10.3969/j.issn.1000-1565.2016.04.010

河北大学学报(自然科学版) ›› 2016, Vol. 36 ›› Issue (4): 387-395.DOI: 10.3969/j.issn.1000-1565.2016.04.010

Box-Behnken设计响应面法优化超声波辅助双水相法提取玛咖多糖

王全¹,李红亚¹,李术娜¹,王会²,李茉莉³

收稿日期:2015-08-20 出版日期:2016-07-25 发布日期:2016-07-25
作者简介:王全(1980—),男,河北衡水人,河北农业大学讲师,河北大学在读博士,主要从事天然药物提取与分析的研究. E-mail:wangquan210@163.com
基金资助:
国家自然科学基金青年基金资助项目(20905019)

Box-Behnken design for optimized extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method

WANG Quan¹,LI Hongya¹,LI Shuna¹,WANG Hui²,LI Moli³

1.College of Life Science, Agricultural University of Hebei, Baoding 071001, China; 2.Agricultural Machinery Management Station of Hengshui, Hengshui 053000, China; 3.College of Biological Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received:2015-08-20 Online:2016-07-25 Published:2016-07-25

摘要/Abstract

摘要： 利用Box-Behnken设计响应面法优化超声波辅助双水相法提取玛咖多糖的条件.采用玛咖干根为原料,考察了提取温度、提取时间、料液比3个因素对提取结果的影响.在单个变量因子实验基础上,通过采用Box-Behnken设计响应面分析法对玛咖多糖的超声波双水相提取条件进行优化.最佳提取条件为提取温度60 ℃、料液比1∶20(质量比)、提取时间52 min.与二次方程的拟合度在0.815 3,得到玛咖多糖的提取率为15.831%,与实测值15.732%基本一致.Box-Behnken响应面法用于优化超声波辅助双水相法提取玛咖多糖的条件是可行的,模型预测效果较好,优化条件具备可行性.

关键词: 玛咖多糖, Box-Behnken 设计, 超声波提取, 双水相

Abstract: A 17-run Box-Behnken design was used to optimize the extraction conditions of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method.Three factors,namely,extraction temperature,extraction time,and solid-liquid ratio were investigated.The experimental data were fitted to a second-order polynomial equation through multiple regression analysis and examined with the appropriate statistical methods.The adjusted coefficient of determination(R²_Adj)for the model was 0.815 3,and the probability value(P<0.000 1)demonstrated a high significance for the regression model.The optimum extraction conditions were as follows:extraction temperature of 60 ℃,extraction time of 52 min,and solid-liquid ratio of 1∶20.Under these conditions,the mean extraction yield of maca polysaccharides was 15.732%,which was in good agreement with the predicted value of 15.831%.Box-Behnken response surface method is used to optimize extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method,the optimized conditions are feasible,the model predition is good.

Key words: maca polysaccharide, box-Behnken design, ultrasonic extraction, aqueous two-phase

中图分类号:

O629

王全,李红亚,李术娜,王会,李茉莉. Box-Behnken设计响应面法优化超声波辅助双水相法提取玛咖多糖[J]. 河北大学学报(自然科学版), 2016, 36(4): 387-395.

WANG Quan,LI Hongya,LI Shuna,WANG Hui,LI Moli. Box-Behnken design for optimized extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(4): 387-395.

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Box-Behnken设计响应面法优化超声波辅助双水相法提取玛咖多糖

Box-Behnken design for optimized extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method

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