Spatial distribution of arbuscular mycorrhizal fungi and glomalin under Artemisia sphaerocephala along a dune in Saibei desert of North China

doi:10.3969/j.issn.1000-1565.2018.03.007

摘要/Abstract

摘要： 以内蒙古青格勒图元上都的典型沙化梁地为研究样地,2013年8月于梁地阳面的底、坡和顶样地,分5个土层(0~10、10~20、20~30、30~40、40~50 cm)采集沙蒿根围土壤样品及根样,探讨沙蒿根围AM(arbuscular mycorrhizal)真菌在不同坡位上的空间分布特征及与土壤因子之间的关系,进一步分析风蚀水蚀的影响. 结果表明,AM真菌的定殖率和孢子密度与样地和采样深度密切相关,梁底显著高于梁坡和梁顶,随着土层加深,孢子密度显著降低.AM真菌总定殖率平均为82.8%,土壤孢子密度平均为2.86/g. 不同样地,随坡位变化,由梁底到梁顶土壤养分含量逐渐降低,梁底土壤有机碳、碱解氮、有效磷含量明显优于梁坡和梁顶;在不同样地间,随土层加深土壤养分含量也逐渐降低;梁底球囊霉素含量明显高于梁坡和梁顶,且随土层加深,球囊霉素含量显著降低. 沙蒿根围总球囊霉素在土壤中的平均质量分数为1.570 mg/g,在土壤有机碳中的质量分数为21.12%.相关性分析表明,孢子密度及AM真菌总定殖率与土壤湿度、有机碳、有效磷、碱解氮、球囊霉素、磷酸酶呈极显著正相关(P<0.01).

关键词: AM真菌, 土壤因子, 球囊霉素, 沙蒿, 梁地

Abstract: The study was carried out in Zhenglanqi, Inner Mongolia. The soil samples in the rhizosphere of Artemisia sphaerocephala were collected from the three sites(the top-dune, mid-dune, and foot-dune)along one typical dune in August 2013, and divided into five depth intervals,0—10, 10—20, 20—30, 30—40 and 40—50 cm,respectively. The results indicated that A. sphaerocephala formed strong symbiotic relationship with AM(arbuscular mycorrhizal)fungi.AM fungal spore density and colonization rates were significantly affected by sampling sites and soil depths.The arbuscular colonization, the spore density and the content of glomalin at the bottom of the slope were significantly higher than those at the middle and top. The spore density gradually decreased with soil depths.The mean total infection of AM fungi was 82.8%, and the spore mean density was 2.86/g. The soil nutrients content were significantly different- DOI:10.3969/j.issn.1000-1565.2018.03.007塞北梁地沙蒿根围AM真菌和球囊霉素空间分布特征贺学礼, 张亚娟, 赵丽莉, 张娟, 许伟(河北大学生命科学学院,河北保定 071002)摘要:以内蒙古青格勒图元上都的典型沙化梁地为研究样地,2013年8月于梁地阳面的底、坡和顶样地,分5个土层(0~10、10~20、20~30、30~40、40~50 cm)采集沙蒿根围土壤样品及根样,探讨沙蒿根围AM(arbuscular mycorrhizal)真菌在不同坡位上的空间分布特征及与土壤因子之间的关系,进一步分析风蚀水蚀的影响. 结果表明,AM真菌的定殖率和孢子密度与样地和采样深度密切相关,梁底显著高于梁坡和梁顶,随着土层加深,孢子密度显著降低.AM真菌总定殖率平均为82.8%,土壤孢子密度平均为2.86/g. 不同样地,随坡位变化,由梁底到梁顶土壤养分含量逐渐降低,梁底土壤有机碳、碱解氮、有效磷含量明显优于梁坡和梁顶;在不同样地间,随土层加深土壤养分含量也逐渐降低;梁底球囊霉素含量明显高于梁坡和梁顶,且随土层加深,球囊霉素含量显著降低. 沙蒿根围总球囊霉素在土壤中的平均质量分数为1.570 mg/g,在土壤有机碳中的质量分数为21.12%.相关性分析表明,孢子密度及AM真菌总定殖率与土壤湿度、有机碳、有效磷、碱解氮、球囊霉素、磷酸酶呈极显著正相关(P<0.01).关键词:AM真菌;土壤因子;球囊霉素;沙蒿;梁地中图分类号:Q948 文献标志码:A 文章编号:1000-1565(2018)03-0268-10Spatial distribution of arbuscular mycorrhizal fungi and glomalin underArtemisia sphaerocephala along a dune in Saibei desert of North ChinaHE Xueli,ZHANG Yajuan, ZHAO Lili, ZHANG Juan, XU Wei(College of Life Sciences,Hebei University,Baoding 071002, China)Abstract: The study was carried out in Zhenglanqi, Inner Mongolia. The soil samples in the rhizosphere of Artemisia sphaerocephala were collected from the three sites(the top-dune, mid-dune, and foot-dune)along one typical dune in August 2013, and divided into five depth intervals,0—10, 10—20, 20—30, 30—40 and 40—50 cm,respectively. The results indicated that A. sphaerocephala formed strong symbiotic relationship with AM(arbuscular mycorrhizal)fungi.AM fungal spore density and colonization rates were significantly affected by sampling sites and soil depths.The arbuscular colonization, the spore density and the content of glomalin at the bottom of the slope were significantly higher than those at the middle and top. The spore density gradually decreased with soil depths.The mean total infection of AM fungi was 82.8%, and the spore mean density was 2.86/g. The soil nutrients content were significantly different- 收稿日期:2017-09-25 基金项目:国家自然科学基金资助项目(31270460) 第一作者:贺学礼(1964—),男,陕西蒲城人,河北大学教授,博士生导师,主要从事生态学研究.E-mail:xuelh1256@126.com第3期贺学礼等:塞北梁地沙蒿根围AM真菌和球囊霉素空间分布特征among the three studied sites and different soil layers. The contents of soil organic carbon, nitrogen, phosphorus and potassium at the bottom of the slope were significantly higher than those at the middle and top. The soil nutrient contents significantly decreased with the depth of soil layer. The average contents of TG(total glomalin)were 1.570 mg / g accounting for 21.12% of the total soil organic carbon. The results of relativity analysis showed AM fungi total colonization and the spore density had significant positive relationship with soil moisture, SOC(soil organic carbon), available P, available N, EEG(easily extractable glomalin), TG, alkaline phosphatase and acid phosphatase(all P<0.01).

Key words: arbuscular mycorrhizal(AM)fungi, soil factor, glomalin, Artemisia sphaerocephala, slope

中图分类号:

Q948

贺学礼, 张亚娟, 赵丽莉, 张娟, 许伟. [J]. 河北大学学报(自然科学版), 2018, 38(3): 268-277.

HE Xueli,ZHANG Yajuan, ZHAO Lili, ZHANG Juan, XU Wei. Spatial distribution of arbuscular mycorrhizal fungi and glomalin under Artemisia sphaerocephala along a dune in Saibei desert of North China[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(3): 268-277.

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