AM真菌、水分和施磷水平对丹参生长及品质的影响

doi:10.3969/j.issn.1000-1565.2016.05.010

摘要/Abstract

摘要： 利用盆栽实验考察不同水分和施磷水平下接种AM真菌对丹参幼苗生长及品质的影响.通过测定丹参生长量、全氮、全磷、丹参酮Ⅱ_A和丹参多糖的含量来研究丹参幼苗的生长及品质.结果发现,相同水分及施磷水平下,接菌株的株高、根长、根冠比、全氮、全磷、丹参酮Ⅱ_A和丹参多糖的含量显著高于未接菌株.相同水分不同施磷水平下,随施磷量的增加,丹参株高、根长、根冠比、全氮、全磷、丹参酮Ⅱ_A和丹参多糖的含量均先升高后下降,在第二施磷水平(P₂)为0.15 g/kg时达到最大,全氮和全磷质量分数最大分别为1.842%和0.354%.不同水分相同施磷水平下,接种株丹参酮Ⅱ_A和丹参多糖的含量显著高于未接菌株,70%田间持水量下丹参酮Ⅱ_A和丹参多糖含量较高.70%田间持水量及施磷质量分数为0.15 g/kg时接种株的丹参酮Ⅱ_A和丹参多糖质量分数最大,分别为0.252%和6.166%,较对照组分别提高0.016%和1.516%.

关键词: AM真菌, 丹参, 磷, 丹参酮Ⅱ_A, 多糖

Abstract: Pot experiments were conducted to study the effects of AM fungi on growth and quality of Salvia miltiorrhiza under different soil moisture and phosphorus levels.The plant height,root length,root shoot ration,total N,total P,tanshinone Ⅱ_A and polysaccharide were determinated to evaluate the quality of S.miltiorrhiza seedlings.The results showed that inoculation of AM fungi significantly increased the plant height,root length,root shoot ratio,total N,total P,tanshinone Ⅱ_A and polysaccharide content of S.miltiorrhiza under the same soil moisture and phosphorus level.Under the same soil moisture and different phosphorus levels,plant height,root length,root shoot ratio,total N,total P,tanshinone Ⅱ_A and polysaccharide content of S.miltiorrhiza increased first and then decreased with increasing phosphorus application rate,and they reached the maximum at second phosporus levels(P₂)was 0.15 g/kg.The content of total N,total P respectively was 1.842% and 0.354%.Under different soil moisture and the same phosphorus levels,tanshinone Ⅱ_A and polysaccharide of inoculated plants were significantly higher than non-inoculated plants.The content of tanshinone - 2016年河北大学学报(自然科学版) 2016第36卷第5期 Journal of Hebei University(Natural Science Edition) Vol.36 No.5DOI:10.3969/j.issn.1000-1565.2016.05.010AM真菌、水分和施磷水平对丹参生长及品质的影响齐俊香,杨晓宇,王紫月,杜天舒,李建恒(河北大学药学院,河北保定 071002)摘要:利用盆栽实验考察不同水分和施磷水平下接种AM真菌对丹参幼苗生长及品质的影响.通过测定丹参生长量、全氮、全磷、丹参酮Ⅱ_A和丹参多糖的含量来研究丹参幼苗的生长及品质.结果发现,相同水分及施磷水平下,接菌株的株高、根长、根冠比、全氮、全磷、丹参酮Ⅱ_A和丹参多糖的含量显著高于未接菌株.相同水分不同施磷水平下,随施磷量的增加,丹参株高、根长、根冠比、全氮、全磷、丹参酮Ⅱ_A和丹参多糖的含量均先升高后下降,在第二施磷水平(P₂)为0.15 g/kg时达到最大,全氮和全磷质量分数最大分别为1.842%和0.354%.不同水分相同施磷水平下,接种株丹参酮Ⅱ_A和丹参多糖的含量显著高于未接菌株,70%田间持水量下丹参酮Ⅱ_A和丹参多糖含量较高.70%田间持水量及施磷质量分数为0.15 g/kg时接种株的丹参酮Ⅱ_A和丹参多糖质量分数最大,分别为0.252%和6.166%,较对照组分别提高0.016%和1.516%.关键词:AM真菌;丹参;磷;丹参酮Ⅱ_A;多糖中图分类号:R282.71 文献标志码:A 文章编号:1000-1565(2016)05-0509-08Effect of AM fungi,moisture,and phosphorus levelson the growth and quality of Salvia miltiorrhiza Bge. QI Junxiang,YANG Xiaoyu,WANG Ziyue,DU Tianshu,LI Jianheng(College of Pharmaceutical Sciences,Hebei University,Baoding 071002,China)Abstract: Pot experiments were conducted to study the effects of AM fungi on growth and quality of Salvia miltiorrhiza under different soil moisture and phosphorus levels.The plant height,root length,root shoot ration,total N,total P,tanshinone Ⅱ_A and polysaccharide were determinated to evaluate the quality of S.miltiorrhiza seedlings.The results showed that inoculation of AM fungi significantly increased the plant height,root length,root shoot ratio,total N,total P,tanshinone Ⅱ_A and polysaccharide content of S.miltiorrhiza under the same soil moisture and phosphorus level.Under the same soil moisture and different phosphorus levels,plant height,root length,root shoot ratio,total N,total P,tanshinone Ⅱ_A and polysaccharide content of S.miltiorrhiza increased first and then decreased with increasing phosphorus application rate,and they reached the maximum at second phosporus levels(P₂)was 0.15 g/kg.The content of total N,total P respectively was 1.842% and 0.354%.Under different soil moisture and the same phosphorus levels,tanshinone Ⅱ_A and polysaccharide of inoculated plants were significantly higher than non-inoculated plants.The content of tanshinone - 收稿日期:2015-10-25 基金项目:河北大学科研基金资助项目(3333112) 第一作者:齐俊香(1992—),女,河北衡水人,河北大学在读硕士研究生,主要从事天然植物有效成分研究.E-mail:714548328@qq.com 通信作者:李建恒(1964-),男,河北徐水人,河北大学药学院教授,博士,主要从事天然植物有效成分研究.E-mail:lijianheng@hbu.cn第5期齐俊香等:AM真菌、水分和施磷水平对丹参生长及品质的影响Ⅱ_A and polysaccharide was higher at 70% WHC.With at 70% WHC and phosphorus application rate of 0.15 g/kg they reached the maximum.The maximum content of tanshinone Ⅱ_A and polysaccharides respectively was 0.252% and 6.166%,which respectively increased 0.016% and 1.516% compared with the control group.

Key words: AM fungi, Salvia miltiorrhiza Bge., phosphorus, tanshinone Ⅱ_A, polysaccharides

中图分类号:

R282.71

齐俊香,杨晓宇,王紫月,杜天舒,李建恒. AM真菌、水分和施磷水平对丹参生长及品质的影响[J]. 河北大学学报(自然科学版), 2016, 36(5): 509-516.

QI Junxiang,YANG Xiaoyu,WANG Ziyue,DU Tianshu,LI Jianheng. Effect of AM fungi,moisture,and phosphorus levels on the growth and quality of Salvia miltiorrhiza Bge.[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(5): 509-516.

参考文献

[1] 国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2010: 70.
[2] 张湘东,许定舟,李金华,等.丹参多糖的免疫调节活性研究[J].中药材,2012,35(6):949-952.DOI:10.13863/j.issn1001-4454.2012.06.032. ZHANG X D,XU D Z,LI J H,et al.Study on the immunocompetence of polysaccharide extracted from root of Salvia miltiorrhiza[J].Journal of Chinese Medicinal Materials,2012,35(6):949-952.DOI:10.13863/j.issn1001-4454.2012.06.032.
[3] 吴巧攀,乔洪翔,何厚洪,等.银杏叶渣中多糖的提取及其抗氧化活性研究[J].中国现代应用药学,2014,31(1): 9-13.DOI:10.13748/j.cnki.issn1007-7693.2014.01.012. WU Q P,QIAO H X,HE H H,et al.Extraction of polysaccharides from residue of Ginkgo biloba leaves and study on its anti-oxidant activity[J].Chinese Journal of Modern Applied Pharmacy,2014,31(1):9-13.DOI:10.13748/j.cnki.issn1007-7693.2014.01.012.
[4] 郭宝林,冯毓秀,赵杨景.丹参种质资源研究进展[J].中国中药杂志,2002,27(7):492-495. GUO B L,FENG Y X,ZHAO Y J.Review of germplasm resources studies on Salvia miltiorrhiza[J].China Journal of Chinese Materia Medica,2002,27(7):492-495.
[5] DUPRE H,JONER E J,LEYVAL C,et al.Role and influence of mycorrhizal fungi on radiocesium accumulation by plants[J].J Environ Radioact,2008,99(5):785-800.DOI:10.1016/j.jenvrad.2007.10.008.
[6] BENNETT A E,BEVER J D,DEANE B M.Arbuscular mycorrhizal fungal species suppress inducible plant responses and alter defensive strategies following herbivory[J].Oecologia,2009,160:771-779.DOI:10.1007/s00442-009-1338-5.
[7] SINGH S,PANDEY A,KUMAR B,et al.Enhancement in growth and quality parameters of tea [Camellia sinensis(L.)O.Kuntze] through inoculation with arbuscular mycorrhizal fungi in an acid soil[J].Biol Ferti Soils,2010,46(5): 427-433.DOI:10.1007/s00374-010-0448-x.
[8] 冯海燕,冯固,王敬国,等.植物磷营养状况对丛枝菌根真菌生长及代谢活性的调控[J].菌物系统,2003,22(4):589-598.DOI:10.13346/j.mycosystema.2003.04.016. FENG H Y,FENG G,WANG J G,et al.Regulation of P statua in host plant on alkaline phosphatase(ALP)activity in intraradical hyphae and development of extraradical hyphae of AM fungi[J].Mycosystema,2003,22(4):589-598.DOI:10.13346/j.mycosystema.2003.04.016.
[9] 滕华容.AM真菌与施磷量对柴胡生长和化学成分交互效应的研究[D].杨凌:西北农林科技大学,2005. TENG H R.Effect of AM fungi on the gowth and chemical composition of Bupleurum chinense DC.under different phosphor applied amount[D].Yangling:North West Agriculture and Forestry University,2005.
[10] 陈梅梅,陈保冬,王新军,等.不同磷水平土壤接种丛枝菌根真菌对植物生长和养分吸收的影响[J].生态学报,2009,29(4):1980-1986. CHEN M M,CHEN B D,WANG X J,et al.Influences of arbuscularmycorrhizal fungi(AMF)on the growth and ecological stoichiometry of clover and ryegrass grown in monoculture or in m ixture at different phosphorus(P)levels[J].Acta Ecologica Sinica,2009,29(4):1980-1986.
[11] 孙向伟,王晓娟,陈牧,等.生态环境因子对AM真菌孢子形成与分布的作用机制[J].草业学报,2011,20(1):214-221. SUN X W,WANG X J,CHEN M,et al.Effects of eco-environmental factors on the production and distribution of arbuscular mycorrhizal fungal spores[J].Acta Prataculturae Sinica,2011,20(1):214-221.
[12] 贺学礼,马丽,孟静静,等.不同水肥条件下AM真菌对丹参幼苗生长和营养成分的影响[J].生态学报,2012,32(18):5721-5728.DOI: 10.5846/stxb201108181216. HE X L,MA L,MENG J J,et al.Effects of AM fungi on the growth and nutrients of Salvia miltiorrhiza Bge.under different soil water and fertilizer conditions[J].Acta ecologica sinica,2012,32(18): 5721-5728.DOI: 10.5846/stxb201108181216.
[13] WU Q S,HE X H,ZOU Y N,et al.Spatial distribution of glomalin-related soil protein and its relationships with root mycorrhization,soil aggregates,carbohydrates,activity of protease and β-glucosidase in the rhizosphere of Citrus unshiu[J].Soil Biology and Biochemistry,2012,45:181-183.DOI:10.1016/j.soilbio.2011.10.002.
[14] 高俊凤.植物生理学实验技术[M].西安:世界图书出版公司,2000.
[15] 徐光域,颜军,郭晓强,等.硫酸-苯酚定糖法的改进与初步应用[J].食品科学,2005,26(8):342-346. XU G Y,YAN J,GUO X Q,et al.The betterment and apply of phenol-sulphate acid method[J].Food Science,2005,26(8):342-346.
[16] JANOS D P,SCHROEDER M S,SCHAFFER B,et al.Inoculation with arbuscular mycorrhizal fungi enhances growth of Litchi chinensis Sonn.trees after propagation by air-layering[J].Plant and Soil,2001,233(1):85-94.
[17] 贺学礼,马丽,王平,等.AM真菌和施P量对黄芩生长、养分吸收和微量元素的影响[J].中国中药杂志,2011,36(16): 2170-2175. HE X L,MA L,WANG P,et al.Effects of AM fungi on growth and microelement of Scuteliaria baiealensis under different applied phosphorous levels[J].China Journal of Chinese Materia Medica,2011,36(16):2170-2175.
[18] 韩建萍,梁宗锁.氮、磷对丹参生长及丹参素和丹参酮Ⅱ_A积累规律研究[J].中草药,2005,36(5):756-759. HAN J P,LIANG Z S.Regulation of Salvia miltiorrhizae growth and danshensu and tanshionⅡ_A accumulation under nitrogen and phosphorus[J].Chinese Traditional and Herbal Drugs,2005,36(5):756-759.
[19] BRAUNBERGER P G,MILLER M H,PETERSON R L.Effect of phosphorus nutrition on morphological characteristics of vesicular arbuscular mycorrhizal colonization of maize[J].New Phytologist,1991,119:107-113.
[20] 刘大会,郭兰萍,黄璐琦,等.土壤水分含量对丹参幼苗生长及有效成分的影响[J].中国中药杂志,2011,36(3):321-325. LIU D H,GUO L P,HUANG L Q,et al.Effects of soil water content on the seedlings growth and active ingredients of Salvia miltiorrhiza Bge[J].China Journal of Chinese Materia Medica,2011,36(3):321-325.