单增李斯特菌对小鼠滋养层细胞炎性体的激活

doi:10.3969/j.issn.1000-1565.2022.01.010

河北大学学报(自然科学版) ›› 2022, Vol. 42 ›› Issue (1): 73-77.DOI: 10.3969/j.issn.1000-1565.2022.01.010

单增李斯特菌对小鼠滋养层细胞炎性体的激活

李文艳^1,2,陈滢滢¹,董子怡¹,刘婕¹,肖锋¹,张宇航¹

收稿日期:2021-04-10 发布日期:2022-02-22
作者简介:李文艳(1973— ),女,河北清苑人,河北大学副教授,博士,主要从事生殖生物学研究.E-mail: liwenyan358@163.com
基金资助:
河北省医学科学研究课题计划项目(20210111);河北大学研究生创新资助项目(HBU2021ss024);河北大学2022年大学生创新创业训练计划项目

Activiation of the inflammasomes in trophoblastic cells by Lisetria monocytogenes

LI Wenyan^1,2, CHEN Yingying¹, DONG Ziyi¹, LIU Jie¹, XIAO Feng¹, ZHANG Yuhang¹

1.College of Medicine, Hebei University, Baoding 071000, China; 2.Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory Autoimmune Diseases in Hebei Province, Baoding 071000, China

Received:2021-04-10 Published:2022-02-22

摘要/Abstract

摘要： 为探讨单增李斯特菌(Lisetria monocytogenes, LM)诱导小鼠滋养层细胞炎性体激活在其致小鼠流产中的作用,在体外用LM处理小鼠滋养层细胞,观察LM在细胞内的分布,检测培养基中白细胞介素(interleukin,IL)-1β水平和细胞裂解液中天冬氨酸蛋白水解酶1(cysteinyl aspartate specific proteinase 1,caspase 1)成熟片段水平以及乳酸脱氢酶(lactate dehydrogenase,LDH)释放量.结果显示:LM分布在细胞核周围,LM感染组细胞培养基中IL-1β水平、细胞裂解液中caspase 1成熟片段水平以及LDH释放量与对照组相比明显增加,溶血素O(listeriolysin O,LLO)缺陷型LM(Δhly LM)感染组IL-1β水平和caspase 1成熟片段水平明显低于野生型LM感染组.因此推测,LM可通过LLO诱导小鼠滋养层细胞炎性体的激活进而诱导IL-1β的分泌和滋养层细胞焦亡导致流产的发生,这可为临床防治LM诱导的流产提供科学依据.

关键词: 单增李斯特菌, 小鼠, 流产, 滋养层细胞, 炎性体

Abstract: To explore the role of Lisetria monocytogenes(LM)-induced mouse trophoblastic cell inflammasome activation in LM-induced mouse abortion, mouse trophoblastic cells were infected with LM. After the mouse trophoblastic cells were infected with LM in vitro,distribution of LM were observed. Interleukin(IL)-1β level in cell medium, cleavaged cysteinyl aspartate specific proteinase 1(caspase 1)level in the cell lysate and the amount of lactate dehydrogenase(LDH)release were detected. Results showed that LM distributed around the cell nucleus. Both IL-1β level in cell medium, cleavaged caspase 1 level in the cell lysate and the amount of LDH release were increased significantly in LM-infected group compared to control group, while the IL-1β level and the cleavaged caspase 1 level of the listeriolysin O(LLO)-deficient LM(Δ hly LM)-infected group were obviously lower than that of wild LM-infected group. Therefore, it is speculated that LM can activiate mouse trophoblastic inflammasome by LLO and promote the secretion of IL-1β and the pyrotosis of trophoblastic cells, which induced the mouse abortion. These results can provide a scientific basis for the clinical prevention and treatment on LM-induced abortion.

Key words: Listeria monocytogenes, mouse, abortion, trophoblastic cell, inflammasome

中图分类号:

S852.61

李文艳,陈滢滢,董子怡,刘婕,肖锋,张宇航. 单增李斯特菌对小鼠滋养层细胞炎性体的激活[J]. 河北大学学报(自然科学版), 2022, 42(1): 73-77.

LI Wenyan, CHEN Yingying, DONG Ziyi, LIU Jie, XIAO Feng, ZHANG Yuhang. Activiation of the inflammasomes in trophoblastic cells by Lisetria monocytogenes[J]. Journal of Hebei University(Natural Science Edition), 2022, 42(1): 73-77.

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单增李斯特菌对小鼠滋养层细胞炎性体的激活

Activiation of the inflammasomes in trophoblastic cells by Lisetria monocytogenes

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