河北大学学报(自然科学版) ›› 2022, Vol. 42 ›› Issue (1): 68-72.DOI: 10.3969/j.issn.1000-1565.2022.01.009

• • 上一篇    下一篇

菌糠资源化开发中激素类物质检测及利用

杨林英,徐凡,耿培妍,尹显达,耿丹萌,王谦   

  • 收稿日期:2020-05-27 发布日期:2022-02-22
  • 通讯作者: 王谦(1962—)
  • 作者简介:杨林英(1996—),女,河南洛阳人,河北大学在读硕士研究生,主要从事大型真菌研究.E-mail:1553431871@qq.com
  • 基金资助:
    河北省第二期现代农业产业技术体系创新团队项目(HBCT2018050204)

Detection and utilization of hormones in the exploitation of mushroom bran resources

YANG Linying, XU Fan, GENG Peiyan, YIN Xianda, GENG Danmeng, WANG Qian   

  1. Biotechnology Technology Innovation Center, Hebei University, Baoding 071002, China
  • Received:2020-05-27 Published:2022-02-22

摘要: 为缓解大量的废弃菌糠造成的面源污染,研究菌糠的资源化开发.以黑木耳菌糠为底物,通过分光光度法检测黑木耳菌糠中赤霉素和吲哚乙酸的含量,结果发现:赤霉素质量分数为45.52 μg/g,加标回收率达98.28%;吲哚乙酸质量分数为38.42 μg/g,加标回收率为94.70%.利用黑木耳菌糠与菜园土、砂质土按不同质量配比混匀种植紫叶生菜,结果发现,与对照相比,一定比例的黑木耳菌糠的使用,可以提高发芽率、缩短发芽时间;当黑木耳菌糠添加量为35%(质量分数)时,栽培出来的紫叶生菜发芽率最高,达到86.91%,且发芽时间最短,约为7 d,表明其具有资源化开发价值.

关键词: 黑木耳菌糠, 赤霉素, 吲哚乙酸, 分光光度法, 紫叶生菜

Abstract: In order to alleviate the non-point source pollution caused by a large amount of waste mushroom bran, to study the resource development of mushroom bran, using Auricularia auricula bran as substrate, the content of gibberellins and indoleacetic acid in A. auricula bran was determined by spectrophotometry.The results showed that the mass fraction of gibberellin was 45.52 μg/g, and the recovery rate was 98.28%; the mass fraction of indole acetic acid was 38.42 μg/g, and the recovery rate was 94.70%.Using A. auricula bran mixed with vegetable garden soil and sandy soil in different quality ratios to plant purple leaf lettuce, it was found that compared with the control, the use of a certain proportion of A. auricula bran can increase the germination rate and shorten the germination time; when the addition amount of A. auricula bran is 35%(mass fraction),the content of the maximum germination rate is 86.91% in purple leaf lettuce,the shortest average germination time is about 7 d, indicating that A. auricula bran has the value for resource development.

Key words: bran of Auricularia auricula, gibberellins, indoleacetic acid, spectrophotometer, purple leaf lettuce

中图分类号: