UPLC-Q-TOF/MS法鉴定兽药磺胺二甲嘧啶在小鼠血液和尿液中的代谢产物

doi:10.3969/j.issn.1000-1565.2015.02.005

河北大学学报(自然科学版) ›› 2015, Vol. 35 ›› Issue (2): 131-137.DOI: 10.3969/j.issn.1000-1565.2015.02.005

UPLC-Q-TOF/MS法鉴定兽药磺胺二甲嘧啶在小鼠血液和尿液中的代谢产物

孙汉文^{1^{,孟哲^{1,2^{,石志红^{1^{，吕运开¹}}}}}}

河北大学化学与环境科学学院, 河北省分析科学技术实验室, 保定071002 2宁夏大学能源化工重点实验室, 银川753200

收稿日期:2015-06-04 修回日期:2015-06-04 出版日期:2015-03-25 发布日期:2015-03-25
作者简介:孙汉文（1945-），男，河北魏县人，河北大学教授，博士生导师，主要从事光学分析，分离科学技术、环境分析化学和农兽药残留分析研究

Received:2015-06-04 Revised:2015-06-04 Online:2015-03-25 Published:2015-03-25

摘要/Abstract

摘要： 本研究采用UPLC-Q-TOF/MS联用技术鉴定了兽药磺胺二甲嘧啶在小鼠血浆和尿液样品中的代谢产物。并运用Q-TOF质谱仪进行MS和MS/MS精确质量数的测定，得到磺胺二甲嘧啶和各个代谢产物的准确分子质量和化学组成。依据精确质量数据，结合Metabolynx XS辅助软件鉴定兽药磺胺二甲嘧啶在ESI源正离子模式下的代谢产物。研究结果表明，磺胺二甲嘧啶在小鼠的血样和尿样中主要的目标代谢途径为乙酰化反应，非目标代谢途径主要为甲基化和结合反应。

关键词: 兽药, 磺胺二甲嘧啶, UPLC-Q-TOF/MS, 代谢产物

Abstract: In the present study, the metabolic profile of sulfamethazine in vivo, a veterinary drug, was investigated using ultra-performance liquid chromatography coupled to high resolution quadrupole time-of-flight mass spectrometry to acquire structural information on sulfamethazine metabolites. The corresponding product ion spectra were acquired and interpreted, and structures were proposed. Accurate mass measurement using ESI-Q-TOF/MS was using to determine the elemental composition of metabolites, thereby confirming the proposed structures of these metabolites. This study demonstrated that acetylating metabolic reaction was predominantly observed in mice plasma and urine, including target metabolite N4-Acetylsulfamethazine, subcardinal metabolites come from methylating renction and conjugates reaction.

Key words: veterinary drug, sulfamethazine, UPLC-Q-TOF/MS, metabolite

中图分类号:

孙汉文,孟哲，石志红，吕运开. UPLC-Q-TOF/MS法鉴定兽药磺胺二甲嘧啶在小鼠血液和尿液中的代谢产物[J]. 河北大学学报(自然科学版), 2015, 35(2): 131-137.

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UPLC-Q-TOF/MS法鉴定兽药磺胺二甲嘧啶在小鼠血液和尿液中的代谢产物

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