透明质酸接枝普鲁兰糖的制备与表征

doi:10.3969/j.issn.1000-1565.2017.02.006

河北大学学报(自然科学版) ›› 2017, Vol. 37 ›› Issue (2): 141-146.DOI: 10.3969/j.issn.1000-1565.2017.02.006

透明质酸接枝普鲁兰糖的制备与表征

杨文智,王树根,刘娇艳,贾蓓,李海鹰

收稿日期:2016-08-10 出版日期:2017-03-25 发布日期:2017-03-25
作者简介:杨文智(1972—),男,内蒙古锡林浩特人,河北大学副教授,从事生物医用材料及药物缓控释制剂方面研究. E-mail:wenzhi_yang@sina.com
基金资助:
河北省高等学校科学技术研究项目(ZD2016102);河北大学研究生创新项目(X2016071)

Preparation and characterization of a new hyaluronic acid grafted pullulan material

YANG Wenzhi,WANG Shugen,LIU Jiaoyan,JIA Bei,LI Haiying

College of Pharmacy, Hebei University, Baoding 071002, China

Received:2016-08-10 Online:2017-03-25 Published:2017-03-25

摘要/Abstract

摘要： 采用化学交联法,以1-(3-二甲氨基丙基)-3-乙基碳二亚胺(EDC)为脱水剂、4-二甲基氨基吡啶(DMAP)为催化剂,将透明质酸(Hyaluronic acid,HA)接枝到普鲁兰(Pullulan,Pu)糖长链上,制备透明质酸-普鲁兰糖(HA-Pu)新型材料.傅里叶变换红外和氢核磁表征显示,成功合成HA-Pu材料,并用¹H NMR法确定HA-Pu的透明质酸取代度.合成新材料冻干后可压制获得HA-Pu膜,扫描电镜下观察,HA-Pu膜为层状结构,具备很多微小孔洞.体外酶降解实验表明,新型HA-Pu膜较透明质酸具有更好的抵抗酶降解性能.HA-Pu新材料合成有望拓展透明质酸在医药领域的应用.

关键词: 透明质酸, 普鲁兰糖, 化学接枝, 体外酶降解

Abstract: A series of hyaluronic acid grafted pullulan(HA-Pu)were prepared by esterification reaction,using 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide(EDC)as dehydrating agent and 4-dimethylaminopyridine(DMAP)as catalyst.The structure of HA-Pu was confirmed by the Fourier transform infrared spectrum(FTIR)and proton nuclear magnetic resonance(¹H NMR),and the degree of substitution(DS)of hyaluronic acid moiety was determined by ¹H NMR.The freeze-dried HA-Pu materials could be compressed to obtain a HA-Pu film.The HA-Pu film was observed by scanning electron microscopy(SEM),which obtained the layered microstructure with a lot of small holes.Compared with hyaluronic acid,the HA-Pu materials have better ability to resist enzyme degradation in vitro.Therefore,HA-Pu should be a promising novel material to expand the application of hyaluronic acid in pharmaceatics.

Key words: hyaluronic acid, pullulan, chemical grafting, enzyme degradation in vitro

中图分类号:

O658

杨文智,王树根,刘娇艳,贾蓓,李海鹰. 透明质酸接枝普鲁兰糖的制备与表征[J]. 河北大学学报(自然科学版), 2017, 37(2): 141-146.

YANG Wenzhi,WANG Shugen,LIU Jiaoyan,JIA Bei,LI Haiying. Preparation and characterization of a new hyaluronic acid grafted pullulan material[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(2): 141-146.

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透明质酸接枝普鲁兰糖的制备与表征

Preparation and characterization of a new hyaluronic acid grafted pullulan material

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