盐效应对还原变性溶菌酶聚集的影响

doi:10.3969/j.issn.1000-1565.2018.06.006

河北大学学报(自然科学版) ›› 2018, Vol. 38 ›› Issue (6): 588-595.DOI: 10.3969/j.issn.1000-1565.2018.06.006

盐效应对还原变性溶菌酶聚集的影响

贾宝环, 于洋,王悦,刘思阳,杜保安,马刚

收稿日期:2017-09-21 出版日期:2018-11-25 发布日期:2018-11-25
通讯作者: 马刚(1971—),男,北京市人,河北大学教授,主要从事与蛋白纤维化聚集相关的研究.E-mail:gangma@hbu.edu.cn杜保安(1963—),男,河北安国人,河北大学教授,主要从事量子点合成与应用研究.E-mail:baoandu@sina.com
作者简介:贾宝环(1991—),女,河北张家口人,河北大学在读硕士研究生,主要从事与蛋白纤维化聚集相关的研究. E-mail:412598250@qq.com
基金资助:
国家自然科学基金资助项目(21075027);河北省自然科学基金资助项目(B2011201082;B2016201034);河北大学创新资助项目(hbu2018ss20)

Salt effects on the aggregation of reduced-denatured lysozyme

JIA Baohuan, YU Yang, WANG Yue, LIU Siyang, DU Baoan, MA Gang

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China

Received:2017-09-21 Online:2018-11-25 Published:2018-11-25

摘要/Abstract

摘要： 以还原变性溶菌酶作为一种蛋白模型体系,研究了硫酸钠(Na₂SO₄)、高氯酸钠(NaClO₄)、硝酸钠(NaNO₃)、氯化钠(NaCl)4种无机盐对蛋白聚集的影响.应用浊度法研究了能引起蛋白聚集的临界盐浓度和不同盐对蛋白聚集动力学的影响. 发现临界盐浓度依照SO₄^2- > ClO₄^- >NO₃^- > Cl^-顺序增加,蛋白聚集速率依照SO₄^2- > ClO₄^- > NO₃^- > Cl^-顺序减小,且对某种特定的盐而言,蛋白聚集的动力学参数和盐浓度呈现良好的线性关系.应用扫描电镜技术对蛋白聚集体的微观形貌进行了表征.发现4种盐都能促进蛋白形成无定型结构的聚集体.本研究对理解盐效应因素对蛋白聚集的影响有一定的借鉴意义.

关键词: 溶菌酶, 蛋白聚集, 盐效应, 浊度法

Abstract: Using reduced-denatured lysozyme as a model protein, the effects of sodium sulfate(Na₂SO₄), sodium perchlorate(NaClO₄), sodium nitrate(NaNO₃), and sodium chloride(NaCl)on protein aggregation were investigated. Turbidity assay were used to study the critical salt concentration on protein aggregation and the aggregation kinetics. It was found that the critical salt concentrations increased by following the order of SO₄^2- > ClO₄^- > NO₃^- > Cl^- and the aggregation rates decreased by following the order of SO₄^2- > ClO₄^- > NO₃^- > Cl^-.The fitted kinetic parameters showed a linear relationship with salt concentrations. All of the four salts induced the formation of amorphous protein aggregates as revealed by scanning electron microscopy. This work should be beneficial for a better understanding of protein aggregation.

Key words: lysozyme, protein aggregation, salt effect, turbidity assay

中图分类号:

O643

贾宝环, 于洋,王悦,刘思阳,杜保安,马刚. 盐效应对还原变性溶菌酶聚集的影响[J]. 河北大学学报(自然科学版), 2018, 38(6): 588-595.

JIA Baohuan, YU Yang, WANG Yue, LIU Siyang, DU Baoan, MA Gang. Salt effects on the aggregation of reduced-denatured lysozyme[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(6): 588-595.

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盐效应对还原变性溶菌酶聚集的影响

Salt effects on the aggregation of reduced-denatured lysozyme

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