黄顶菊入侵不同时间对土壤微生物功能的影响

doi:10.3969/j.issn.1000-1565.2021.02.011

摘要/Abstract

摘要： 通过同质园试验,采用Biolog技术比较黄顶菊(Flaveria bidentis)定植不同时间(5年、7年、9年)其与狗尾草根际土壤微生物对碳源的利用能力,分析其功能多样性的变化.结果表明:定植不同时间,黄顶菊与本地植物狗尾草混合种植小区(简称F+S处理)的平均颜色变化率都高于黄顶菊单一种植小区(简称F处理);与其他处理相比,F+S处理的均匀度指数U较高;随着定植时间的延长,F+S处理的均匀度指数U显著增加;F处理的优势度指数D显著增加,其均匀度指数U逐渐下降,并在定植9年时显著小于狗尾草单一种植小区(简称S处理);与其他处理相比,F+S处理促进了土壤微生物对脂类、糖类和氨基酸的吸收,与定植5年和7年相比,定植9年F+S处理的土壤微生物对脂类的利用能力显著提高,对糖类利用能力显著降低.由此可知,黄顶菊入侵后定植不同时间对土壤微生物的功能产生了影响.因此,探究外来植物根际土壤微生物功能随时间变化的一般规律,有助于进一步揭示外来植物的入侵机制.

关键词: 黄顶菊, 土壤微生物, 功能多样性, 竞争生长, Biolog

Abstract: By using the homologous garden test, Biolog technique was used to compare the utilization of carbon sources in the rhizosphere soil microorganisms of Flaveria bidentis and Setaria viridis and analyzed the changes of functional diversity at different times(5 years, 7 years and 9 years).The results showed that:After different time of invasion,the average well color development(AWCD)values of F+S treatments were higher than those of F treatment under different invasive years.Compared with other treatments,the McIntosh index U of F+S treatment was higher;with the increase of invasion time,the McIntosh index U of F+S treatment increased significantly;the Simpson index D of the F treatment increased significantly,its McIntosh index U gradually decreased and was significantly smaller than the S treatment at 9 years of colonization.Compared with other treatments, F+S treatment promoted the absorption of lipids, sugars and amino acids by soil microorganisms. Compared to colonization for 5 and 7 years,- DOI:10.3969/j.issn.1000-1565.2021.02.011黄顶菊入侵不同时间对土壤微生物功能的影响王妍,杜鄂巍,蒙彦良,孙建茹,桑晓玲,张风娟(河北大学生命科学学院,河北保定 071002)摘要:通过同质园试验,采用Biolog技术比较黄顶菊(Flaveria bidentis)定植不同时间(5年、7年、9年)其与狗尾草根际土壤微生物对碳源的利用能力,分析其功能多样性的变化.结果表明:定植不同时间,黄顶菊与本地植物狗尾草混合种植小区(简称F+S处理)的平均颜色变化率都高于黄顶菊单一种植小区(简称F处理);与其他处理相比,F+S处理的均匀度指数U较高;随着定植时间的延长,F+S处理的均匀度指数U显著增加;F处理的优势度指数D显著增加,其均匀度指数U逐渐下降,并在定植9年时显著小于狗尾草单一种植小区(简称S处理);与其他处理相比,F+S处理促进了土壤微生物对脂类、糖类和氨基酸的吸收,与定植5年和7年相比,定植9年F+S处理的土壤微生物对脂类的利用能力显著提高,对糖类利用能力显著降低.由此可知,黄顶菊入侵后定植不同时间对土壤微生物的功能产生了影响.因此,探究外来植物根际土壤微生物功能随时间变化的一般规律,有助于进一步揭示外来植物的入侵机制.关键词:黄顶菊;土壤微生物;功能多样性;竞争生长;Biolog中图分类号:Q948.11 文献标志码:A 文章编号:1000-1565(2021)02-0180-08Effects of different invasion time of Flaveria bidentis on soil microbial function WANG Yan, DU Ewei, MENG Yanliang, SUN Jianru, SANG Xiaoling, ZHANG Fengjuan(College of Life Sciences, Hebei University, Baoding 071002, China)Abstract: By using the homologous garden test, Biolog technique was used to compare the utilization of carbon sources in the rhizosphere soil microorganisms of Flaveria bidentis and Setaria viridis and analyzed the changes of functional diversity at different times(5 years, 7 years and 9 years).The results showed that:After different time of invasion,the average well color development(AWCD)values of F+S treatments were higher than those of F treatment under different invasive years.Compared with other treatments,the McIntosh index U of F+S treatment was higher;with the increase of invasion time,the McIntosh index U of F+S treatment increased significantly;the Simpson index D of the F treatment increased significantly,its McIntosh index U gradually decreased and was significantly smaller than the S treatment at 9 years of colonization.Compared with other treatments, F+S treatment promoted the absorption of lipids, sugars and amino acids by soil microorganisms. Compared to colonization for 5 and 7 years,- 收稿日期:2020-04-16 基金项目:国家自然科学基金资助项目(31972343);河北省自然科学基金资助项目(C2019201059);河北大学研究生创新项目(hbu2019ss026) 第一作者:王妍(1995—),女,山东济南人,河北大学硕士研究生,主要从事入侵生物学研究.E-mail:1007276126@qq. com 通信作者:张风娟(1969—),女,河北唐山人,河北大学教授,博士,主要从事植物学与生态学方向研究.E-mail:Fengjuanzhang@126.com第2期王妍等:黄顶菊入侵不同时间对土壤微生物功能的影响soil microorganisms in F+S treatment significantly improved the utilization of lipids and reduced the utilization of sugars in F. bidentis colonization for 9 years, respectively. It can be seen that the different times of F. bidentis colonization have an impact on the function of soil microorganism.Therefore, exploring the general laws of the function of alien plant rhizosphere soil microorganisms over time will help to further reveal the invasion mechanism of alien plants.

Key words: Flaveria bidentis, soil microbes, functional diversity, competitive growth, Biolog

中图分类号:

Q948.11

王妍,杜鄂巍,蒙彦良,孙建茹,桑晓玲,张风娟. 黄顶菊入侵不同时间对土壤微生物功能的影响[J]. 河北大学学报(自然科学版), 2021, 41(2): 180-187.

WANG Yan, DU Ewei, MENG Yanliang, SUN Jianru, SANG Xiaoling, ZHANG Fengjuan. Effects of different invasion time of Flaveria bidentis on soil microbial function[J]. Journal of Hebei University(Natural Science Edition), 2021, 41(2): 180-187.

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