半乳糖酞菁光动力治疗结肠癌的实验研究

doi:10.3969/j.issn.1000-1565.2017.02.012

摘要/Abstract

摘要： 为了探讨新型光敏剂半乳糖酞菁(T1—T4)对结肠癌细胞CT-26增殖的影响及其相关机制,通过检测T1—T4的水溶性,利用荧光分光光度计检测T1—T4的浓度和孵育时间对CT-26细胞吸收量的影响.MTT(四甲基偶氮唑盐)法检测T1—T4对CT-26的细胞毒作用,利用MDC(单丹磺酰尸胺)染色法和流式细胞术检测结肠癌细胞的自噬、凋亡和坏死情况.结果发现T1—T4的水溶性随半乳糖取代基个数的增加而增强,对CT-26细胞的光动力杀伤作用与其浓度呈正相关并且细胞CT-26对T1—T3的吸收量随着孵育浓度的升高和孵育时间的延长而增加,对T4几乎无吸收.T2可诱导CT-26细胞出现自噬、晚期凋亡和坏死,且有较明显的剂量依赖性.通过以上数据了解到 CT-26对T1—T3的吸收随药物浓度和孵育时间的增加而升高,T2介导的光动力疗法对CT-26有明显抑制作用,其机制可能与诱导细胞自噬有关.

关键词: 酞菁, 光动力疗法, 自噬, 凋亡

Abstract: To explore the effect and its related mechanism of new photosensitizers galactose substituted phthalocyanines(T1—T4)on the proliferation of colon cancer cell CT-26,consequently to provide experimental basis for the clinical photodynamic therapy of colon cancer,the water distribution coefficient of T1—T4 was detected to understand its water-soluble ability,the concentration and incubation time of T1—T4 were detected by fluorescence spectrophotometer to know their influence on CT-26 cell absorbing capacity,the cytotoxic effect of T1—T4 to CT-26 was detected by MTT the autophagy,apoptosis and necrosis of the colon cancer cells were detected through MDC staining and flow cytometry.It was found that the- water-soluble ability of T1—T4 enhanced,with the number of galactose substituents increasing.The photodynamic damage effect of T1—T4 to CT-26 was positively correlated with their concentration.The absorptive amount of CT-26 to T1—T3 increased with the concentration of incubation and with the extension of incubation time,but it can hardly absorb T4.Autophagy,apoptosis and necrosis of CT-26 can be induced by T2,and there is obvious dose-dependent effect.Through the above data, colon cancer cell absorbs more T1—T3 when the incubation concentration and incubation time increase.T2 mediated photodynamic therapy can obviously inhibit CT-26.The mechanism may be due to cell autophagy.

Key words: phthalocyanine, photodynamic therapy, autophagy, apoptosis

中图分类号:

X835

葛伟颖,赵占娟,洪阁,赵建喜. 半乳糖酞菁光动力治疗结肠癌的实验研究[J]. 河北大学学报(自然科学版), 2017, 37(2): 180-186.

GE Weiying,ZHAO Zhanjuan,HONG Ge,ZHAO Jianxi. Experiment research of photodynamic therapy of colon cancer with a novel photosensitizer galactose substituted phthalocyanines[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(2): 180-186.

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