关键词: 光动力抗菌化学疗法, 创伤感染, 光敏剂, 卟啉衍生物

Abstract: The aim of this study is to investigate the photodynamic antibacterial activity of new porphyrin-based photosensitizers, and provide the experimental basis for the application of photodynamic antibacterial in clinic.Three strains were cultured in vitro MRSA, Escherichia coli, and Pseudomonas aeruginosa, respectively,and the uptake of five compounds(PA1—PA5)by three bacteria was tested.The light reaction and dark reaction concentration curves of the PA1—PA5 against three bacteria were determined by colony counting method, and laser confocal microscopy was used to detect bacterial phagocytosis of photosensitizers.All of the five porphyrins had relatively slow photobleaching rate and strong singlet-state generation ability.The results of laser confocal microscopy also showed that the three types of bacteria- DOI:10.3969/j.issn.1000-1565.2017.04.010新型水溶性卟啉类光敏剂的体外抗菌作用赵占娟¹,耿文一²,樊紫瑜¹,洪阁³,刘天军³(1.河北大学 医学院,河北 保定 071002;2.暨南大学 医学院, 广东 广州 510632;3.中国医学科学院 生物医学工程研究所,天津市生物医学材料重点实验室,天津 300192)摘 要:为了探讨新型卟啉类光敏剂光动力体外灭菌的活性,体外培养了抗药性金黄色葡萄球菌(MRSA)、大肠杆菌(Escherichia coli)和铜绿假单胞菌(Pseudomonas aeruginosa)3种菌株,分别检测3种细菌对5种卟啉化合物(PA1—PA5)的吸收量,菌落计数法检测PA1—PA5对3种细菌的光反应、暗反应浓度曲线;激光共聚焦显微镜检测细菌吞噬光敏剂的情况.结果发现,5种卟啉类化合物均具有较缓慢的光漂白率和较强的单线态氧产生能力.激光共聚焦显微镜的检测结果也表明,3类菌株可以选择性地吸收化合物PA1—PA5.相比于其他4种光敏剂,PA1表现出明显的杀菌性能,当PA1浓度增加为31.25 μmol/L时,光照PA1对3种细菌的灭活率达100%.PA1对3种细菌的光动力灭菌效果最好,未来具有良好的使用前景.关键词:光动力抗菌化学疗法;创伤感染;光敏剂;卟啉衍生物中图分类号:O43;Q631;R641 文献标志码:A 文章编号:1000-1565(2017)04-0393-07In vitro study on antimicrobial activity of newwater-soluble porphyrin photosensitizersZHAO Zhanjuan¹,GENG Wenyi²,FAN Ziyu¹,HONG Ge³,LIU Tianjun³(1.Medical College, Hebei University, Baoding 071002, China; 2.Medical College, Jinan University, Guangzhou 510632, China;3.Key Laboratory of Biomedical Mater of Tianjin,Institute of BiomedicalEngineering, Chinese Academy of Medical Sciences,Tianjin 300192,China)Abstract: The aim of this study is to investigate the photodynamic antibacterial activity of new porphyrin-based photosensitizers, and provide the experimental basis for the application of photodynamic antibacterial in clinic.Three strains were cultured in vitro MRSA, Escherichia coli, and Pseudomonas aeruginosa, respectively,and the uptake of five compounds(PA1—PA5)by three bacteria was tested.The light reaction and dark reaction concentration curves of the PA1—PA5 against three bacteria were determined by colony counting method, and laser confocal microscopy was used to detect bacterial phagocytosis of photosensitizers.All of the five porphyrins had relatively slow photobleaching rate and strong singlet-state generation ability.The results of laser confocal microscopy also showed that the three types of bacteria- 收稿日期:2016-09-02 基金项目:河北大学医学学科建设项目(2015A2001);中西部高校综合实力提升工程专项经费;天津市生物医学材料重点实验室开放性课题 第一作者:赵占娟(1974—),女,河北博野人,河北大学副教授,博士,主要从事激光医学研究.E-mail: zhaozhanjuanv@163.com 通信作者:刘天军(1965—),男,河南辉县人,中国医学科学院生物医学工程研究所研究员,博士生导师,主要从事分子设计方面的研究.E-mail:liutianjun@hotmail.com第4期赵占娟等:新型水溶性卟啉类光敏剂的体外抗菌作用were selective for the uptake of PA1—PA5.PA1 showed significant bactericidal properties compared to the other four photosensitizers.With irradiation PA1 on three bacteria,the inactivation rate was 100%,when the concentration was increased to 31.25 μmol/L.PA1 as a new type of antibacterial photosensitizer shows a broad prospect as it shows the best effect on photodynamic sterilization of three bacteria.

Key words: photodynamic antimicrobial chemotherapy, wound infection, photosensitizer, porphyrin derivative