7-羟基香豆素醛在检测次氯酸根中的应用

doi:10.3969/j.issn.1000-1565.2018.01.005

河北大学学报(自然科学版) ›› 2018, Vol. 38 ›› Issue (1): 28-32.DOI: 10.3969/j.issn.1000-1565.2018.01.005

7-羟基香豆素醛在检测次氯酸根中的应用

阴彩霞¹,熊康明¹,霍方俊²

收稿日期:2017-10-28 出版日期:2018-01-25 发布日期:2018-01-25
作者简介:阴彩霞(1978—),女,山西平遥人,山西大学教授,主要从事有机小分子荧光探针的研究. E-mail:yincx@sxu.edu.cn
基金资助:
国家自然科学基金资助项目(21672131;21775096;21705102);山西省人才支持计划资助项目(2014401);山西省归国基金资助(2017-026)

Application of 7-hydroxylcoumarin-aldehyde in the detection of hypochlorite

YIN Caixia¹, XIONG Kangming¹, HUO Fangjun²

1.Institute of Molecular Science, Shanxi University, Taiyuan 030006, China; 2.Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China

Received:2017-10-28 Online:2018-01-25 Published:2018-01-25

摘要/Abstract

摘要： 7-羟基香豆素醛在PBS(pH=7.4)的缓冲溶液中可以被用于检测次氯酸根.光谱实验结果表明,该探针在PBS(pH=7.4)的缓冲溶液中对次氯酸根的识别具有荧光增强的性质,并伴随着溶液的颜色由浅黄色变为深黄色,溶液的荧光由弱荧光变为深蓝色荧光.探针对次氯酸根的识别响应在1 s内迅速完成,并且检测线可以达到51.75 nmol/L.此外,7-羟基香豆素醛可以在HepG2细胞中对次氯酸根进行荧光成像.

关键词: 7-羟基香豆素醛, 次氯酸根, 荧光增强, 荧光成像

Abstract: 7-hydroxylcoumarin-aldehyde can be used to detect hypochlorite in PBS(pH=7.4)solution.The results of spectra show that the probe has a fluorescence enhancement property for hypochlorite recognition in PBS(pH=7.4)solution, and the color of the solution changes from light yellow to deep-yellow, and the fluorescence of the solution changes from weak fluorescence to deep-blue fluorescence.The recognition response of the probe to hypochlorite can be rapidly completed within 1s, and the detection limit is found to be as low as 51.75 nmol/L.In addition, the probe can be used for fluorescence imaging of hypochlorite in HepG2 cells.

Key words: 7-hydroxylcoumarin-aldehyde, hypochlorite, fluorescence enhancement, fluorescence imaging

中图分类号:

O622.4

阴彩霞,熊康明,霍方俊. 7-羟基香豆素醛在检测次氯酸根中的应用[J]. 河北大学学报(自然科学版), 2018, 38(1): 28-32.

YIN Caixia, XIONG Kangming, HUO Fangjun. Application of 7-hydroxylcoumarin-aldehyde in the detection of hypochlorite[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(1): 28-32.

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7-羟基香豆素醛在检测次氯酸根中的应用

Application of 7-hydroxylcoumarin-aldehyde in the detection of hypochlorite

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