Genomic DNA Extraction and Systematic Study of the Dry Preserved Tribe Platyopini Specimen of China

doi:10.3969/j.issn.1000-1565.2010.02.017

Abstract

Abstract: In order to reduce insect molecular systematics research costs and improve resource utilization,dried specimens to extract genomic DNA were used.DNA was extracted from dried specimens and liquid dip samples of Pimeliinae kept in Museum of Hebei University for different storage life, which was used as template to amplify some specific gene fragments via PCR. No significant difference was noticed between those DNA from different samples. UPGMA phylogenetic tree was made up using the sequence information of Cytochrome B (Cytb) genetic fragments, and which was compared with traditional taxonomy to discuss the phylogenetic relationships of tribe Platyopini. Results support Reitter's opinion(2005) that Platyopini and Pimeliini belong to two tribes, negate Beutle's standpoint(1893) that belong to one tribe. This paper also provides experience for molecular systematic research of using dried coleoptera insect specimens.

Key words: Platyopini, dry preserved specimen, DNA extraction, phylogeny

CLC Number:

Q961.7

AN Wen-ting,REN Guo-dong,LIU Feng-song. Genomic DNA Extraction and Systematic Study of the Dry Preserved Tribe Platyopini Specimen of China[J]. Journal of Hebei University (Natural Science Edition), 2010, 30(2): 190-195,200.

References

[1] 张迎春, 郑哲民, 安书成, Zhang Yingchun, Zheng Zhemin, An Shucheng. 瓢虫DNA的提取研究 [J]. 陕西师范大学学报（自然科学版） 1999.doi:10.3321/j.issn:1672-4291.1999.01.024
[2] 林万华, 黎宗展, 陈振鹏, 周善义, LIN Wan-hua, LI Zong-zhan, CHEN Zhen-peng, ZHOU Shan-yi. 昆虫全基因组DNA的保存及提取 [J]. 广西师范大学学报（自然科学版） 2006.doi:10.3969/j.issn.1001-6600.2006.02.021
[3] 朴美花, 陈学新, 何俊华. 膜翅目昆虫干标本的基因组DNA提取 [J]. 动物分类学报 2002.doi:10.3969/j.issn.1000-0739.2002.04.004
[4] 印红, 刘晓丽, 王彦芳, 张道川, 赵晓瑜. 一种改进的昆虫基因组DNA的提取方法 [J]. 河北大学学报(自然科学版) 2002.doi:10.3969/j.issn.1000-1565.2002.01.021
[5] 张建珍, 郭亚平, 马恩波. 不同保存方式下蝗虫组织DNA的提取及RAPD分析 [J]. 动物学杂志 2004.doi:10.3969/j.issn.0250-3263.2004.02.011
[6] 徐光勇, 陈斌, 吴蔚文, 刘彦群, 鲁成. 天牛干标本DNA的提取及RAPD-PCR扩增反应 [J]. 西南农业大学学报 2003.doi:10.3969/j.issn.1673-9868.2003.02.001
[7] 田英芳, 黄刚, 郑哲民. 一种简易的昆虫基因组DNA提取方法 [J]. 陕西师范大学学报（自然科学版） 1999.doi:10.3321/j.issn:1672-4291.1999.04.021
[8] 张大治, 戴沛捷. 蜻蜓目昆虫DNA的提取方法研究 [J]. 宁夏农学院学报 2004.doi:10.3969/j.issn.1673-0747.2004.03.003
[9] ROHLAND N, SIEDEL H, HOFREIFER M. Nondestructive DNA extraction method for mitochondrial DNA analyses of museum specimens [J]. Biotechniques 2004, 36.
[10] GILBERT M T P, MOORE W, MELCHIOR L. DNA extraction from dry museum beetles without conferring external morphological damage [J]. Public Library of Science Medicine 2007, 3.
[11] Kalmar T, Bachrati CZ, Marcsik A, Rasko I. A simple and efficient method for PCR amplifiable DNA extraction from ancient bones. [J]. Nucleic Acids Research 2000, 12(12).
[12] David R. Maddison, Michael D. Baker, Karen A. Ober. Phylogeny of carabid beetles as inferred from 18S ribosomal DNA (Coleoptera: Carabidae) [J]. Systematic Entomology 1999, 2(2).
[13] BOUCHARD P, LAWRENCE J F, DAVIEs A E. Synoptic classification of the world Tenebrionidae (Insecta:Coleoptera) with a review of family-group names [J]. Annales Zoologici (Warszawa) 2005.
[14] REITTER E. Insecta in itinere Cl.N.Prezwalskii in Asia Centrali novissime lecta.Ⅸ.Tenebrionidae [J]. Horae Societatis Entomologicae Rossicae 1887, 21.
[15] SEMENOW A P. Symbolae ad cognitionem Pimeliidarum [J]. Horae Societatis Entomologicae Rossicae 1893, 27.
[16] REITTER E, Insecta a cl G N. Potamin in China et in mongolia novissime lecta.XIII.Tenebrionidae [J]. Horae Societatis Entomologicae Rossicae 1889, 23.
[17] 钟玉林. 中国螽斯总科部分种类Cytb和28S rDNA分子进化与系统学研究 [D]. 西安:陕西师范大学 2005.