Abstract: The optimization of the extraction process the Ganoderma lucidum polyscaccharides(GLP) was studied. The microwave method under slightly alkaline medium was employed and a single factor analysis and an orthogonal test were used in the study. The order of the effects of the factors influencing the extraction was as follow: extraction time, pH, the mass ratio between G. lucidum and water, presoak time. The optimum condition was: presoak for 1.5 h, extract for 15 min, the mass ratio of G. lucidum and water 1∶110, pH 8.0 and extracted twice. GLP extraction rate of 3.200%, the result indicated that the method can significantly improve the rate of extraction of GLP.

Key words: Ganoderma lucidum polysaccharides, microwave, orthogonal test