Research status of novel gene editing technology of CRISPR/Cas9 system

doi:10.3969/j.issn.1000-1565.2016.01.014

Abstract

Abstract: Clustered regularly interspaced short palindromic repeats(CRISPR)and associated proteins(Cas)comprise the CRISPR/Cas system,which confers adaptive immunity against foreign invading phages,plasmids and other mobile elements in many bacteria and most archaea.On the basis of the species and homology of the Cas protein,CRISPR/Cas systems have been classified into three distinct groups(type Ⅰ~Ⅲ).The component of type Ⅱ CRISPR/Cas9 system is the simplest one,and is well-charactered in resent study.CRISPR/Cas9 system modified by genetic engineering has proven to be an efficient gene-targeting tool in various organisms.Here,the auther review the discovery,components,molecular mechanism,applications,challenges and future prospect of CRISPR/Cas9-mediated genome editing.

Key words: gene editing, CRISPR/Cas9, CRISPRi, interference mechanism, gene therapy

CLC Number:

Q789

QIAN Leiyang,ZHOU Zhijun,CHANG Yanlin. Research status of novel gene editing technology of CRISPR/Cas9 system[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(1): 84-93.

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