Determination of vancomycin and QNs residues in chicken by graphene based-MSPE-liquid chromatography-high-resolution mass spectrometry

doi:10.3969/j.issn.1000-1565.2016.05.006

Abstract

Abstract: A method for the determination of vancomycin and quinolones residues in chicken was developed using liquid chromatography mass spectrometry(LC-MS).The sample was extracted with acetonitrile and purified using MSPE as the sorbent.The target compounds were separated on a Agilent ZORBAX SB-C18 column(3.0 mm×100 mm,1.8 μm)before submitting for detection by high-resolution mass spectrometry.The results showed that high correlation coefficients(r²>0.99)of the analyses were obtained within their respective linear ranges.The average recoveries and the relative standard deviations ranged from 71.2% to 98.8% and 6.8% to 14.3% respectively at the spiked levels of 5,10,25 μg/kg in chicken.This method is reliable,and suitable for the determination of vancomycin and quinolones residues in chicken.

Key words: graphene MSPE, liquid chromatography mass spectrometry, vancomycin, quinolones

CLC Number:

O652

DONG Yanli,LIU Huimin,LI Chao,MA Jingjun,CUI Xiaona. Determination of vancomycin and QNs residues in chicken by graphene based-MSPE-liquid chromatography-high-resolution mass spectrometry[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(5): 480-486.

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