Impacts of rearing mode and photoperiod on the daily rhythms of RNA/DNA ratio in Pelodiscus sinensis

doi:10.3969/j.issn.1000-1565.2017.01.009

Abstract

Abstract: In this study,the impact of photoperiod on daily growth rhythm and the impact of rearing mode on instantaneous growth of Pelodiscus sinensis were investigated.Turtles are grouply housed in fishery while they are separately housed in laboratory experiments.In this experiment,RNA/DNA ratio was chose to reflect the instantaneous growth in P. sinensis.Two photoperiod regimes—12 h light with 12 h dark(12 L∶12 D)and 24 h light with 0 h dark(24 L∶0 D)accompanied with two rearing modes—individual(I)and group(G)were used as treatment factors.Turtles were housed under specific experimental conditions for 15 days,after then five turtles were randomly sampled from each group every 2 h for 24 h.Muscle tissues were dissected for the measurement of RNA/DNA ratio.The results showed that there exists endogenous circadian rhythm of RNA/DNA ratio in P. sinensis.While the rhythm in turtles grouply housed was strongly influenced by photoperiod,the endogenous rhythm was totally masked.And the ratio in separately housed turtles was significantly higher than that in grouply housed turtles.In conclusion,the - DOI:10.3969/j.issn.1000-1565.2017.01.009饲养模式及光周期对中华鳖RNA/DNA比值昼夜节律的影响冀芳烁,杨振才(河北师范大学生命科学学院,河北石家庄 050024)摘要:为了探究光周期对中华鳖(Pelodiscus sinensis)生长昼夜节律的影响及中华鳖养殖中常用的群体养殖模式(群养)与实验室研究中常用的单独养殖模式(单养)对瞬时生长的影响,实验选用RNA/DNA比值反映中华鳖的瞬时生长情况,以光周期(12 L∶12 D与24 L∶0 D)和饲养模式(单养与群养)为处理因素.实验进行15 d后,连续24 h内每隔2 h分别从各组随机取5只鳖,解剖取肌肉组织,测定其RNA/DNA比值.结果表明,中华鳖的RNA/DNA比值存在内源性昼夜节律变化,而群养模式下光周期对该节律的影响更加强烈,完全掩饰了其原本的节律;单养模式下中华鳖的RNA/DNA比值显著高于群养模式.所以在中华鳖相关研究中,单养模式能更好地反映动物内在的生理过程.关键词:中华鳖;RNA/DNA比值;单/群养;光周期中图分类号:Q95 文献标志码:A 文章编号:1000-1565(2017)01-0056-06Impacts of rearing mode and photoperiod on the daily rhythms ofRNA/DNA ratio in Pelodiscus sinensisJI Fangshuo,YANG Zhencai(College of Life Science,Hebei Normal University,Shijiazhuang 050024,China)Abstract:In this study,the impact of photoperiod on daily growth rhythm and the impact of rearing mode on instantaneous growth of Pelodiscus sinensis were investigated.Turtles are grouply housed in fishery while they are separately housed in laboratory experiments.In this experiment,RNA/DNA ratio was chose to reflect the instantaneous growth in P. sinensis.Two photoperiod regimes—12 h light with 12 h dark(12 L∶12 D)and 24 h light with 0 h dark(24 L∶0 D)accompanied with two rearing modes—individual(I)and group(G)were used as treatment factors.Turtles were housed under specific experimental conditions for 15 days,after then five turtles were randomly sampled from each group every 2 h for 24 h.Muscle tissues were dissected for the measurement of RNA/DNA ratio.The results showed that there exists endogenous circadian rhythm of RNA/DNA ratio in P. sinensis.While the rhythm in turtles grouply housed was strongly influenced by photoperiod,the endogenous rhythm was totally masked.And the ratio in separately housed turtles was significantly higher than that in grouply housed turtles.In conclusion,the - 收稿日期:2016-04-15 基金项目:国家自然科学基金资助项目(31172085);河北省研究生创新项目(sj2015006) 第一作者:冀芳烁(1988—),女,河北平山人,河北师范大学在读博士研究生,主要从事水生动物学方面的研究.E-mail:jifangshuo@163.com 通信作者:杨振才(1962—),男,河北行唐人,河北师范大学教授,主要从事水生动物学方面的研究.E-mail:zcyang@hebtu.edu.cn第1期冀芳烁等:饲养模式及光周期对中华鳖RNA/DNA比值昼夜节律的影响individually rearing mode can better reflect the intrinsic physiological process in P. sinensis.

Key words: Pelodiscus sinensis, RNA/DNA ratio, individually/grouply housed, photoperiod

CLC Number:

JI Fangshuo,YANG Zhencai. Impacts of rearing mode and photoperiod on the daily rhythms of RNA/DNA ratio in Pelodiscus sinensis[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(1): 56-61.

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