Inhibition comparison of six antagonistic bacteria against Phytophthora infestans

doi:10.3969/j.issn.1000-1565.2017.02.010

Abstract

Abstract: The six bacterial strains including WL1,WL2,WR1,WR2,WK1 and M15,obtained in previously experiments,had the obvious inhibition to Phytophthora infestans mycelia growth.In order to clarify the biocontrol potential,the inhibition property and the disease prevention effects on in vitro tissue of six strains were compared in this experiment.The dual-culture method and punch method were adopted to determine the inhibition of living bacteria,bacterial liquid,as well as fermented liquid on P.infestans mycelia growth.The potato tuber slices method was used to evaluate the disease prevention effect of bacterial liquid.The results showed that the inhibition effects of six bacterial liquid to P.infestans growth were significantly greater than that of living bacteria and fermented liquid,and the inhibitory zones were above 27 mm,and all of them caused mycelial malformation.In which,bacterial liquid of WL2 strain displayed the strongest inhibitory effect,the inhibitory zones were above 26 mm,and caused the most serious malformation.In- addition,WL2 bacterial liquid acquired the best disease prevention on in vitro tissue in comparison with other 5 strains,the disease index was only 13.7,and the relative protection ratio was up to 80.5%.These results indicate that WL2 strain has more potential in control potato late blight in the future.

Key words: antagonistic bacteria, Phytophthora infestans, inhibition, disease prevention

CLC Number:

WANG Youyou,JIANG Jizhi,LI Ying,ZHANG Yahui,SUN Han,LANG Yafeng. Inhibition comparison of six antagonistic bacteria against Phytophthora infestans[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(2): 169-175.

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