Competition and induction inhibition of bacterium HT-6 on mycelia growth of Phytophthora infestans

doi:10.3969/j.issn.1000-1565.2021.05.014

Abstract

Abstract: In order to deeply understand the antagonistic mechanism of bacterium HT-6 against Phytophthora infestans, the agar column method was used to observe the competition in spatial position between HT-6 strain and P. infestans. Chrome Azurol Sulphonate(CAS)medium was used to survey and determine the production of siderophore. Sulfuric acid anthrone colorimetric method and salicylic acid-concentrated sulfuric acid colorimetry method were used to compare the utilization rates of glucose and nitrate nitrogen, respectively. At the same time, P. infestans and its cell wall preparation(CWP)were used as inducers to induce the cellulase and β-1,3-glucanase produced by HT-6, which were observed based on the plate transparent circle method and measured with DNS method. The inhibition of HT-6 free cell fermentation liquid induced by CWP on P. infestans mycelia growth was evaluated with dual culture method. The results showed that P. infestans mycelia inoculated on PDA column alone could grow along column and spread quickly onto Rye medium, HT-6 docked with P. infestans on the agar column could rapidly grow and spread on the surface of the Rye medium, as well, while P. infestans docked with HT-6 on the agar column grew only in agar column and was inhibited by HT-6; HT-6 could produce siderophore, while P. infestans could not. The utilization rates of glucose(75.66%)and nitrate nitrogen(69.05%)by HT-6 were significantly higher than those of P. infestans(55.97% and 56.87%), respectively. And HT-6 strain could not produce cellulase and β-1,3-glucanase as it was cultured alone, but both P. infestans strain and its CWP could induce HT-6 to produce these two enzymes. Activities of cellulase and β-1,3-glucanase induced by P. infestans strain were 6.638,2.913 U, while those by CWP were 1.326,1.289 U, respectively. There was a significant difference between two induction treatments(P<0.05); HT-6 free cell fermentation liquid containing two enzymes after CWP induction had a significant inhibitory effect on P. infestans mycelia growth, inhibition rate was 33.25%, but it was significantly lower than that of the HT-6 bacterial solution, inhibition rate reached up to 81.33%. It indicated that the bacterium HT-6 can inhibit the P. infestans through both the competition of space and nutrition,and producing the cell wall degrading enzymes induced by P.infestans, in which space and nutrition competition are dominant.

Key words: inhibition mechanism, induction, cell wall, nutrition and space locus competition, Phytophthora infestans, bacterium

CLC Number:

S435.32

JIANG Jizhi, ZHANG Congying, LIANG Jiao, QIAO Liu, MENG Cuili. Competition and induction inhibition of bacterium HT-6 on mycelia growth of Phytophthora infestans[J]. Journal of Hebei University(Natural Science Edition), 2021, 41(5): 572-580.

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