Effect of drought stress on Viburnum sargentii Koehne photosynthetic characteristics

doi:10.3969/j.issn.1000-1565.2019.05.014

Abstract

Abstract: Using Viburnum sargentii Koehne seedlings as test materials, using pot-set four water moisture gradients, correspondins to 95% of water holding capacity the largest field(control), 75%(mild drought), 55%(moderate drought)and 35%(severe drought), the effect of soil moisture on V. sargentii photosynthetic parameters was studied.The results show that:the net photosynthetic rate(P_n), stomatal conductance(G_s)and transpiration rate(T_r)of V. sargentii reduced with soil water decreasing and intercellular mole fraction of CO₂(x_{__{co_{_₂})in mild and moderate drought stress decreased significantly, but increased under severe drought stress. When the drought stress V. sargentii light saturation point(LSP), maximum net photosynthetic- DOI:10.3969/j.issn.1000-1565.2019.05.014干旱胁迫对天目琼花光合特征的影响杨建伟^1,2,3,赵丹¹,孙桂芳¹,刘嘉翔¹,赵艺璇¹,史宝胜¹(1.河北农业大学园林与旅游学院,河北保定 071000;2.兴隆县林业和草原局,河北兴隆 067300;3.兴隆县住房和城乡建设局,河北兴隆 067300)摘要:以天目琼花(Viburnum sargentii Koehne)幼苗为实验材料,采用盆栽控水法设置4个水分梯度,分别为田间最大持水量的95%(对照)、75%(轻度胁迫)、55%(中度胁迫)和35%(重度胁迫),研究土壤水分对天目琼花光合参数的影响.结果表明:天目琼花的净光合速率(P_n)、气孔导度(G_s)和蒸腾速率(T_r)随着土壤水分递减而减少,胞间CO₂摩尔分数(x_{__{co_{_₂})在轻度和中度干旱胁迫时下降,在严重干旱时显著升高.随着胁迫程度的加剧,天目琼花的光饱和点(LSP)、最大净光合速率(P_nmax)、暗呼吸速率(R_d)和表观量子效率(AQY)显著减少,光补偿点(LCP)显著增加(P<0.01).天目琼花的最大荧光(F_m)、最大光化学效率(F_v/F_m)和光系统Ⅱ(PSⅡ)的量子效率(F_v/F_o)随着土壤水分递减而减少,初始荧光(F_o)显著升高(P<0.01);随着土壤含水量的下降能量分配比率参数φ_Po、Ψ_o、φ_Eo逐渐下降,φ_Do逐渐增加;代表单位反应中心活性的参数ABS/RC、TR_o/RC、ET_o/RC和DI_o/RC逐渐升高.研究表明,在轻度和中度干旱胁迫下天目琼花光合作用能力的降低主要是由气孔限制因素引起的,重度干旱主要是非气孔限制因素引起的.关键词:天目琼花;干旱胁迫;光合特征中图分类号:S688 文献标志码:A 文章编号:1000-1565(2019)05-0529-07Effect of drought stress on Viburnum sargentii Koehne photosynthetic characteristicsYANG Jianwei^1,2,3, ZHAO Dan¹,SUN Guifang¹, LIU Jiaxiang¹,ZHAO Yixuan¹, SHI Baosheng¹(1.College of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding 071000,China;2.Forestry and Grassland Bureau of Xinglong County,Xinglong 067300,China;3.County Housing and Urban-Rural Development Bureau of Xinglong,Xinglong 067300,China)Abstract: Using Viburnum sargentii Koehne seedlings as test materials, using pot-set four water moisture gradients, correspondins to 95% of water holding capacity the largest field(control), 75%(mild drought), 55%(moderate drought)and 35%(severe drought), the effect of soil moisture on V. sargentii photosynthetic parameters was studied.The results show that:the net photosynthetic rate(P_n), stomatal conductance(G_s)and transpiration rate(T_r)of V. sargentii reduced with soil water decreasing and intercellular mole fraction of CO₂(x_{__{co_{_₂})in mild and moderate drought stress decreased significantly, but increased under severe drought stress. When the drought stress V. sargentii light saturation point(LSP), maximum net photosynthetic- 收稿日期:2018-06-26 基金项目:河北省林业厅资助项目(1704487);河北省林业科学研究院资助项目(17226320D) 第一作者:杨建伟(1994—),男,河北张家口人,河北农业大学硕士,主要从事园林植物研究.E-mail:443801835@qq.com 通信作者:史宝胜(1969—),男,河北高阳人,河北农业大学教授,博士生导师,主要从事园林植物栽培与育种研究.E-mail:baoshengshi@163.com第5期杨建伟等:干旱胁迫对天目琼花光合特征的影响rate(P_nmax), dark respiration rate(R_d)and apparent quantum efficiency(AQY)significantly reduced, light compensation point(LCP)increased significantly(P <0.01).The maximum fluorescence(F_m), maximum photochemical efficiency(F_v/F_m)and PSⅡ quantum efficiency(F_v/F_o)decreased with the soil water decreasing, and the initial fluorescence(F_o)(P < 0.01)was significantly increased.When the drought stress energy distribution ratio parameters,φ_Po、Ψ_o、φ_Eo gradually decline, heass ipation ratio of quantum φ_Do increase gradually.As the drought stress intensifies, the parameters of plant unit reaction center activity ABS/RC, TR_o/RC, Et_o/RC and DI_o/RC increase gradually. Studies have shown that:the decrease of the photosynthesis was mainly caused by stomatal factors, and the net photosynthetic rate was caused by the non-stomatal factors in severe drought stress.}}}}}}

Key words: Viburnum sargentii Koehne, drought stress, photosynthetic characteristics

CLC Number:

S688

YANG Jianwei, ZHAO Dan,SUN Guifang, LIU Jiaxiang,ZHAO Yixuan, SHI Baosheng. Effect of drought stress on Viburnum sargentii Koehne photosynthetic characteristics[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(5): 529-535.

References

[1] 王瑗, 盛连喜, 李科, 等. 中国水资源现状分析与可持续发展对策研究[J]. 水资源与水工程学报, 2008, 19(3): 10-14.
[2] 金英杰. 浅谈我国水资源短缺的现状及节水措施[J]. 中国高新技术企业, 2009(15): 137-138. DOI:10.3969/j.iT3n.1009-2374.2009.15.076.
[3] 张建国,李吉跃,沈国舫.树木耐旱特性及其机理研究[M].北京:中国林业出版社, 2000.
[4] 李春燕, 王进鑫, 薛设. 干旱及复水对侧柏幼苗叶片抗氧化酶活性的影响[J]. 西北林学院学报, 2015, 30(2): 33-37. DOI:10.3969/j.iT3n.1001-7461.2015.02.06.
[5] 周朝彬, 宋于洋, 王炳举, 等. 干旱胁迫对胡杨光合和叶绿素荧光参数的影响[J]. 西北林学院学报, 2009, 24(4): 5-9.
[6] 汤飞洋, 金荷仙, 唐宇力. 不同程度干旱胁迫对4个杜鹃品种叶绿素荧光参数的影响[J]. 西北林学院学报, 2017, 32(5): 64-68, 108. DOI:10.3969/j.iT3n.1001-7461.2017.05.12.
[7] 王华玺. 天目琼花嫩枝扦插技术[J]. 农村经济与科技, 2018, 29(2): 36. DOI:10.3969/j.iT3n.1007-7103.2018.02.024.
[8] 吕征, 刘树梅, 宋书芳, 等. 不同处理对天目琼花扦插繁殖的影响[J]. 山东林业科技, 2015, 45(6): 37-40. DOI:10.3969/j.iT3n.1002-2724.2015.06.009.
[9] 马晓华,郑坚,张旭乐,等.不同扦插基质与激素对荚莲生根的影响[J].浙江农业科学,2018,59(5):811-813.DOI:10.16178/j.iT3n.0528-9017.20180541
[10] 杨捷, 朱玉菲, 何笑蕾, 等. 低温胁迫对天目琼花和花叶锦带抗寒生理的影响[J]. 河北农业大学学报, 2015, 38(2): 60-65. DOI:10.13320/j.cnki.jauh.2015.0035.
[11] 罗桑卓玛, 辛福梅, 杨小林, 等. 干旱胁迫对香柏幼苗生长和生理指标的影响[J]. 西北农林科技大学学报(自然科学版), 2015, 43(5): 51-57. DOI:10.13207/j.cnki.jnwafu.2015.05.024.
[12] 叶子飘. 光合作用对光和CO₂响应模型的研究进展[J]. 植物生态学报, 2010, 34(6): 727-740. DOI:10.3773/j.iT3n.1005-264x.2010.06.012.
[13] SOFO A, DICHIO B, MONTANARO G, et al. Photosynthetic performance and light response of two Olive cultivars under different water and light regimes[J]. Photosynthetica, 2009, 47(4): 602-608. DOI:10.1007/s11099-009-0086-4.
[14] DAVIES W J, ZHANG J. Root signaT1 and the regulation of growth and development of plants in drying soil[J]. Ann Rev Physiol Plant Mol Biol,1991, 42(1): 55-76. DOI:10.1146/annurev.pp.42.060191.000415.
[15] FARQUHAR G D, SHARKEY T D. Stomatal conductance and photosynthesis[J]. Annual Review of Plant Physiology, 1982, 33(1): 317-345. DOI:10.1146/annurev.pp.33.060182.001533.
[16] CHEN Y, XU D Q. Two patterns of leaf photosynthetic response to irradiance transition from saturating to limiting one in some plant species[J]. New Phytologist, 2006, 169(4): 789-798. DOI:10.1111/j.1469-8137.2005.01624.x.
[17] 李志军, 罗青红, 伍维模, 等. 干旱胁迫对胡杨和灰叶胡杨光合作用及叶绿素荧光特性的影响[J]. 干旱区研究, 2009, 26(1): 45-52. DOI:10.13866/j.azr.2009.01.019.
[18] 姬兰柱, 肖冬梅, 王淼. 模拟水分胁迫对水曲柳光合速率及水分利用效率的影响[J]. 应用生态学报, 2005, 16(3): 408-412. DOI:10.13287/j.1001-9332.2005.0459.
[19] 叶子飘, 于强. 光合作用光响应模型的比较[J]. 植物生态学报, 2008, 32(6): 1356-1361. DOI:10.3773/j.iT3n.1005-264x.2008.06.016.
[20] 陆燕元, 马焕成, 李昊民, 等. 土壤干旱对转基因甘薯光合曲线的响应[J]. 生态学报, 2015, 35(7): 2155-2160. DOI:10.5846/stxb201306041308.
[21] XIA J B,ZHANG S Y,ZHANG G C, et al.Critical responses of photosynthetic efficiency in Campsis radicans(L.)Seem to soil water and light intensities[J]. African Journal of Biotechnology, 2011, 10(77): 17748-17754. DOI:10.5897/ajb11.2208.
[22] 杨建伟,孙桂芳,赵丹,等.干旱胁迫对麻叶绣线菊光合及荧光参数的影响[J].河北农业大学学报,2018,41(3):49-54.DOI:10.13320/j.cnki.jauh.2018.0054.
[23] 倪霞, 曹永慧, 周本智, 等. 干旱处理对毛竹光响应的影响: 基于4种模型比较分析[J]. 林业科学研究, 2017, 30(3): 465-471. DOI:10.13275/j.cnki.lykxyj.2017.03.015.
[24] 卜令铎, 张仁和, 常宇, 等. 苗期玉米叶片光合特性对水分胁迫的响应[J]. 生态学报, 2010, 30(5): 1184-1191.
[25] OUKARROUM A, SCHANSKER G, STRAT3ER R J. Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chla fluorescence kinetics in barley varieties differing in their drought tolerance[J]. Physiologia Plantarum, 2009, 137(2): 188-199. DOI:10.1111/j.1399-3054.2009.01273.x.
[26] 李娟, 彭镇华, 高健, 等. 干旱胁迫下黄条金刚竹的光合和叶绿素荧光特性[J]. 应用生态学报, 2011, 22(6): 1395-1402. DOI:10.13287/j.1001-9332.2011.0198.
[27] 冯胜利, 马富裕, 方志刚, 等. 水分胁迫对加工番茄光系统Ⅱ的影响[J]. 干旱地区农业研究, 2009, 27(1): 163-167.