Photodynamic antibacterial and antitumor activity of photosensitizer T1

doi:10.3969/j.issn.1000-1565.2019.05.013

Abstract

Abstract: To investigate the photodynamic therapy(PDT)effect of the same photosensitivities on tumor cells and drug-resistant bacteria, colon cancer cells(CT-26)and methicillin-resistant Staphylococcus aureus(MRSA)selected in vitro. The phagocytosis of photosensitizer T1 by MRSA and CT-26 was detected by laser confocal microscopy. The light and dark response curves of T1 to CT-26 cells and MRSA were determined by MTT staining and colony counting. The results showed that both CT-26 cells and MRSA absorbed T1 rapidly in a time-dependent and concentration-dependent manner. T1-mediated PDT showed strong inhibition on CT-26 cells and MRSA, and CT-26 cells and MRSA showed strong fluorescence signal of T1 by confocal laser microscopy. The proliferation of MRSA and CT-26 cells were significantly inhibited through T1-mediated PDT, and PDT may become a new method for the treatment of tumors which caused by bacterial infection in the future, providing experimental data for the clinical application of PDT.- DOI:10.3969/j.issn.1000-1565.2019.05.013光敏剂T1的光动力抗菌与抗肿瘤活性赵占娟¹,马莹莹²,刘亚萌¹,杨焱惜¹,商亚贞³,赵建喜³(1. 河北大学医学院,河北保定 071002;2.河北大学公共卫生学院,河北保定 071002;3.河北大学附属医院放射科,河北保定 071002)摘要:为了探讨同种光敏剂对肿瘤细胞及耐药细菌的光动力(photodynamic therapy,PDT)治疗效果,体外选取了金黄色葡萄球耐药菌(methicillin-resistant Staphylococcus aureus, MRSA)及结肠癌细胞(CT-26),采用激光共聚焦显微镜检测MRSA及CT-26对半乳糖酞菁类光敏剂T1的吞噬,四甲基偶氮唑蓝染色法(MTT法)和菌落计数法检测T1对CT-26细胞和MRSA的光反应及暗反应浓度曲线;结果发现CT-26细胞及MRSA对T1吸收迅速且呈时间和浓度依赖性. T1介导的PDT对CT-26细胞和MRSA均具有较强的抑制,激光共聚焦显微镜也显示T1在CT-26细胞和MRSA内都有很强的荧光信号.T1介导的PDT能明显抑制MRSA及CT-26细胞的增殖,未来有可能成为一种治疗由细菌感染引起肿瘤的新方法,为PDT的临床应用提供实验数据.关键词:光敏剂;光动力疗法;细菌;肿瘤细胞中图分类号:R735 文献标志码:A 文章编号:1000-1565(2019)05-0522-07Photodynamic antibacterial and antitumor activity of photosensitizer T1ZHAO Zhanjuan¹, MA Yingying², LIU Yameng¹, YANG Yanxi¹, SHANG Yazhen³, ZHAO Jianxi³(1. College of Basic Medical Science, Hebei University, Baoding 071002, China;2. College of Public Health, Hebei University, Baoding 071002, China;3. Department of Radiology, Affiliated Hospital of Hebei University, Baoding 071002, China)Abstract: To investigate the photodynamic therapy(PDT)effect of the same photosensitivities on tumor cells and drug-resistant bacteria, colon cancer cells(CT-26)and methicillin-resistant Staphylococcus aureus(MRSA)selected in vitro. The phagocytosis of photosensitizer T1 by MRSA and CT-26 was detected by laser confocal microscopy. The light and dark response curves of T1 to CT-26 cells and MRSA were determined by MTT staining and colony counting. The results showed that both CT-26 cells and MRSA absorbed T1 rapidly in a time-dependent and concentration-dependent manner. T1-mediated PDT showed strong inhibition on CT-26 cells and MRSA, and CT-26 cells and MRSA showed strong fluorescence signal of T1 by confocal laser microscopy. The proliferation of MRSA and CT-26 cells were significantly inhibited through T1-mediated PDT, and PDT may become a new method for the treatment of tumors which caused by bacterial infection in the future, providing experimental data for the clinical application of PDT.- 收稿日期:2019-03-04 基金项目:河北省卫生厅资助项目(20170833);河北大学大学生创新训练计划项目(2018307;2019027);河北大学医学院2018年实验室开放项目第一作者:赵占娟(1974—),女,河北博野人,河北大学副教授,博士,主要从事激光医学方向研究. E-mail:zhaozhanjuanv@163.com 通信作者:赵建喜(1969—),男,河北保定人,河北大学教授,主要从事影像诊断方向研究.E-mail:jianxizhaov@163.com第5期赵占娟等:光敏剂T1的光动力抗菌与抗肿瘤活性

Key words: photosensitizer, photodynamic therapy, bacteria, tumor cell

CLC Number:

R735

ZHAO Zhanjuan, MA Yingying, LIU Yameng, YANG Yanxi, SHANG Yazhen, ZHAO Jianxi. Photodynamic antibacterial and antitumor activity of photosensitizer T1[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(5): 522-528.

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