Journal of Hebei University (Natural Science Edition) ›› 2019, Vol. 39 ›› Issue (5): 522-528.DOI: 10.3969/j.issn.1000-1565.2019.05.013

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Photodynamic antibacterial and antitumor activity of photosensitizer T1

ZHAO Zhanjuan1, MA Yingying2, LIU Yameng1, YANG Yanxi1, SHANG Yazhen3, ZHAO Jianxi3   

  1. 1. College of Basic Medical Science, Hebei University, Baoding 071002, China; 2. College of Public Health, Hebei University, Baoding 071002, China; 3. Department of Radiology, Affiliated Hospital of Hebei University, Baoding 071002, China
  • Received:2019-03-04 Online:2019-09-25 Published:2019-09-25

Abstract: To investigate the photodynamic therapy(PDT)effect of the same photosensitivities on tumor cells and drug-resistant bacteria, colon cancer cells(CT-26)and methicillin-resistant Staphylococcus aureus(MRSA)selected in vitro. The phagocytosis of photosensitizer T1 by MRSA and CT-26 was detected by laser confocal microscopy. The light and dark response curves of T1 to CT-26 cells and MRSA were determined by MTT staining and colony counting. The results showed that both CT-26 cells and MRSA absorbed T1 rapidly in a time-dependent and concentration-dependent manner. T1-mediated PDT showed strong inhibition on CT-26 cells and MRSA, and CT-26 cells and MRSA showed strong fluorescence signal of T1 by confocal laser microscopy. The proliferation of MRSA and CT-26 cells were significantly inhibited through T1-mediated PDT, and PDT may become a new method for the treatment of tumors which caused by bacterial infection in the future, providing experimental data for the clinical application of PDT.- DOI:10.3969/j.issn.1000-1565.2019.05.013光敏剂T1的光动力抗菌与抗肿瘤活性赵占娟1,马莹莹2,刘亚萌1,杨焱惜1,商亚贞3,赵建喜3(1. 河北大学 医学院,河北 保定 071002;2.河北大学 公共卫生学院,河北 保定 071002;3.河北大学附属医院 放射科,河北 保定 071002)摘 要:为了探讨同种光敏剂对肿瘤细胞及耐药细菌的光动力(photodynamic therapy,PDT)治疗效果,体外选取了金黄色葡萄球耐药菌(methicillin-resistant Staphylococcus aureus, MRSA)及结肠癌细胞(CT-26),采用激光共聚焦显微镜检测MRSA及CT-26对半乳糖酞菁类光敏剂T1的吞噬,四甲基偶氮唑蓝染色法(MTT法)和菌落计数法检测T1对CT-26细胞和MRSA的光反应及暗反应浓度曲线;结果发现CT-26细胞及MRSA对T1吸收迅速且呈时间和浓度依赖性. T1介导的PDT对CT-26细胞和MRSA均具有较强的抑制,激光共聚焦显微镜也显示T1在CT-26细胞和MRSA内都有很强的荧光信号.T1介导的PDT能明显抑制MRSA及CT-26细胞的增殖,未来有可能成为一种治疗由细菌感染引起肿瘤的新方法,为PDT的临床应用提供实验数据.关键词:光敏剂;光动力疗法;细菌;肿瘤细胞中图分类号:R735 文献标志码:A 文章编号:1000-1565(2019)05-0522-07Photodynamic antibacterial and antitumor activity of photosensitizer T1ZHAO Zhanjuan1, MA Yingying2, LIU Yameng1, YANG Yanxi1, SHANG Yazhen3, ZHAO Jianxi3(1. College of Basic Medical Science, Hebei University, Baoding 071002, China;2. College of Public Health, Hebei University, Baoding 071002, China;3. Department of Radiology, Affiliated Hospital of Hebei University, Baoding 071002, China)Abstract: To investigate the photodynamic therapy(PDT)effect of the same photosensitivities on tumor cells and drug-resistant bacteria, colon cancer cells(CT-26)and methicillin-resistant Staphylococcus aureus(MRSA)selected in vitro. The phagocytosis of photosensitizer T1 by MRSA and CT-26 was detected by laser confocal microscopy. The light and dark response curves of T1 to CT-26 cells and MRSA were determined by MTT staining and colony counting. The results showed that both CT-26 cells and MRSA absorbed T1 rapidly in a time-dependent and concentration-dependent manner. T1-mediated PDT showed strong inhibition on CT-26 cells and MRSA, and CT-26 cells and MRSA showed strong fluorescence signal of T1 by confocal laser microscopy. The proliferation of MRSA and CT-26 cells were significantly inhibited through T1-mediated PDT, and PDT may become a new method for the treatment of tumors which caused by bacterial infection in the future, providing experimental data for the clinical application of PDT.- 收稿日期:2019-03-04 基金项目:河北省卫生厅资助项目(20170833);河北大学大学生创新训练计划项目(2018307;2019027);河北大学医学院2018年实验室开放项目 第一作者:赵占娟(1974—),女,河北博野人,河北大学副教授,博士,主要从事激光医学方向研究. E-mail:zhaozhanjuanv@163.com 通信作者:赵建喜(1969—),男,河北保定人,河北大学教授,主要从事影像诊断方向研究.E-mail:jianxizhaov@163.com第5期赵占娟等:光敏剂T1的光动力抗菌与抗肿瘤活性

Key words: photosensitizer, photodynamic therapy, bacteria, tumor cell

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