Abstract: To explore whether Th1 and Th2 cytokines play important roles in Listeria monocytogenes(LM)-induced mouse abortion,the pregnant mice and mouse macrophages were infected with LM. Embryo survival rates were calculated. Th1 and Th2 cytokine levels were analyzed in uterus and medium of mouse macrophages. Results showed that Th1 cytokine levels increased significantly in uterus of abortive mice induced by LM. Embryo survival rates and Th1 and Th2 cytokine levels showed no changes in uterus of mice infected by listeriolysin O(LLO)-deficient LM(Δ hly LM). Th1 cytokine and interleukin(IL)-4 levels increased- DOI:10.3969/j.issn.1000-1565.2020.01.009单增李斯特菌致流产小鼠子宫中Th1和Th2细胞因子的变化 李文艳^1,2,张娜³, 李晓宁¹,刘纯甫¹,梁子腾⁴,仲飞²,管越强³(1.河北大学 基础医学院,河北 保定 071002; 2. 河北农业大学 动物医学院,河北 保定 071000;3.河北大学 生命科学学院,河北 保定 071002;4.河北大学 公共卫生学院,河北 保定 071002)摘 要:为探讨Th1和Th2细胞因子在单增李斯特菌(Listeria monocytogenes,LM)致小鼠流产中的作用,用LM感染妊娠小鼠和小鼠巨噬细胞,统计胚胎存活率,酶联免疫测定(enzyme linked lmmuno sorbent assay,ELISA)小鼠子宫组织中和巨噬细胞培养基中主要的Th1和Th2细胞因子水平.结果显示,LM诱导的流产小鼠子宫中Th1细胞因子水平显著增加,溶血素O(listeriolysin O,LLO)基因缺陷型(Δ hly)LM感染组胚胎存活率和子宫中Th1、Th2细胞因子水平无显著变化;LM感染的巨噬细胞分泌的Th1细胞因子和白细胞介素-4(interleukin, IL-4)水平显著增加,IL-10水平显著降低,Δ hly LM感染组Th1和Th2细胞因子水平无显著变化.因此,LM感染后子宫中Th1/Th2细胞因子失衡诱导了小鼠流产,这可为临床防治LM诱导的流产提供科学依据.关键词:单增李斯特菌;流产;Th1和Th2细胞因子;巨噬细胞中图分类号:S855.1 文献标志码:A 文章编号:1000-1565(2020)01-0057-06Changes of Th1 and Th2 cytokine levels in uterus of abortive miceinduced by Listeria monocytogenes LI Wenyan^1,2, ZHANG Na³, LI Xiaoning¹, LIU Chunfu¹, LIANG Ziteng⁴, ZHONG Fei², GUAN Yueqiang³(1. College of Basic Medicine, Hebei University,Baoding 071002, China; 2. College of Veterinary Medicine, Agriculture University of Hebei,Baoding 071000, China; 3. College of Life Sciences, Hebei University,Baoding 071002, China; 4.College of Public Health, Hebei University,Baoding 071002, China)Abstract: To explore whether Th1 and Th2 cytokines play important roles in Listeria monocytogenes(LM)-induced mouse abortion,the pregnant mice and mouse macrophages were infected with LM. Embryo survival rates were calculated. Th1 and Th2 cytokine levels were analyzed in uterus and medium of mouse macrophages. Results showed that Th1 cytokine levels increased significantly in uterus of abortive mice induced by LM. Embryo survival rates and Th1 and Th2 cytokine levels showed no changes in uterus of mice infected by listeriolysin O(LLO)-deficient LM(Δ hly LM). Th1 cytokine and interleukin(IL)-4 levels increased- 收稿日期:2019-04-01 基金项目:河北省自然科学基金资助项目(C2017201213);河北省现代农业产业技术体系淡水养殖创新团队项目(HBCT2018180203);河北大学中西部高校提升综合实力专项基金资助项目 第一作者:李文艳(1973— ),女,河北清苑人,河北大学副教授,博士,主要从事生殖生物学与分子免疫学研究.E-mail: liwenyan358@163.com 通信作者: 仲飞(1956—),男,河北蔚县人,河北农业大学教授,博士,主要从事分子病毒与分子免疫学研究. E-mail: feizhong2013@126.com第1期李文艳等:单增李斯特菌致流产小鼠子宫中Th1和Th2细胞因子的变化 while IL-10 level decreased in the medium of macrophages infected by wild-type LM. After infected by Δ hly LM, Th1 and Th2 cytokine levels showed no changes. Therefore, LM-induced Th1/Th2 imbalance in uterus can causes the mouse abortion,which provides scientific basis for clinical prevention and treatment of LM-induced abortion.

Key words: Listeria monocytogenes, abortion, Th1 and Th2 cytokines, macrophages