Table of Content

25 January 2020, Volume 40 Issue 1

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Dynamic response of hollow hemispherical shell of titanium alloy after thermal oxidation

NIU Xiaoyan, GENG Xuchen, CHEN Cong, SHEN Linlin, ZHANG Zhanbiao

2020, 40(1):  1-6.  DOI: 10.3969/j.issn.1000-1565.2020.01.001

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The finite element program is used to simulate the bullet impact of TC4(Ti-6Al-4V)hot titanium oxide alloy inner hollow hemisphere shell. Based on quasi-static compression test and dynamic Hopkinson pressure bar test data, the effect of diameter-thickness ratio, impact velocity and strain rate on TC4 is studied. The contact force peak and the vertex displacement of the inner hollow hemisphere shell of TC4 hot titanium oxide alloy under different working conditions were obtained. The material model of the TC4 hot titanium oxide alloy is described by the Johnson-Cook constitutive relationship considering the strain rate effect and the temperature effect, where in the test data is measured by Hopkinson pressure bar technology. The results show that the contact force peak and the vertex displacement are inversely proportional to the diameter-thickness ratio; the contact force peak and the vertex displacement are both proportional to the impact velocity; the strain rate effect enhances the impact resistance and the resistance of deformation of the TC4 hot titanium oxide alloy inner hollow hemisphere shell.

Motion of dielectric particles in dust plasma metal ratchet

SONG Chao,DAI Chaoxing,WANG Ruiqi,SUN Wentao,GUO Zhiqiang,LIU Fucheng,HE Yafeng

2020, 40(1):  7-11.  DOI: 10.3969/j.issn.1000-1565.2020.01.002

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The rectification of dust particles in a metal ratchet in plasma environment is studied experimentally. The monodisperse polystyrene particles are suspended in a metal ratchet channel.The direction of particle flows can be controlled by adjusting the gas pressure and the discharge power. In the experiment, only the negative motion of the particles occurs.Due to the low potential of the metal ratchet, the thicker sheath on the surface forms a strong constraint on the particles. Therefore, the nonequilibrium force of ion drag in the azimuthal direction becomes stronger, which results in fast flow of dust particles along the saw channel. The experimental results lay the foundation for the establishment of a dust plasma ratchet model.

Analysis of fluorescence process of DCM in solid matrix environment

XIE Ningbo,YIN Zhengzheng, DENG Ru, DANG Wei

2020, 40(1):  12-17.  DOI: 10.3969/j.issn.1000-1565.2020.01.003

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Although DCM molecules are widely used in the field of lasers, organic light-emitting diodes, and etc., there are relatively few studies on its luminescence properties in solid matrices. In this paper DCM with 1% and 5%weight ratios were dissolved in poly(methyl methacrylate)(PMMA), and its emission process were analyzed at different temperatures. It was found that DCM sample(1% weight ratios)matrix polarity did not show significant change in temperature range of 295-35 K.However, DCM samples of 5%weight ratios, showed reduced matrix polarity in the temperature range of 295-195 K. Both DCM samples show fluorescence intensity and fluorescence lifetime increases with temperature decrease.

Imidazolium-embedded cysteine-functionalized stationary phases: preparation and its chromatographic applications

WANG Shengyu, LIANG Peng, DUAN Liguang, QIAO Xiaoqiang

2020, 40(1):  18-26.  DOI: 10.3969/j.issn.1000-1565.2020.01.004

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A novel imidazolium-embedded cysteine-functionalized stationary phase SIL-AVI-Cys was prepared by “click reaction”, which was further characterized by thermogravimetric analysis, FT-IR as well as elemental analysis. The stationary phase exhibits HILIC mode in the separation of five hydrophilic compounds, such as cytosine, cytidine, inosine, uracil and uridine, which also exhibits HILIC/RPLC mixed mode when separating anilines and phenolic substances. The separation mechanism of the stationary phase was further explored by investigating the effects of mobile phase pH and salt concentration on the retention of analytes. The change in the pH of the mobile phase can affect the interaction between analytes and the stationary phase by varying the dissociation of the analytes. The separation characteristics of the SIL-AVI-Cys column were also investigated by amides and nucleobases/nucleosides. The SIL-AVI-Cys column- DOI:10.3969/j.issn.1000-1565.2020.01.004咪唑嵌合半胱氨酸修饰固定相制备及色谱应用王胜玉¹,梁鹏¹,段立广²,乔晓强¹(1. 河北大学 药学院,河北 保定 071002;2. 河北医科大学第一医院 药学部,河北 石家庄 050000)摘 要:利用“点击反应”合成了咪唑嵌合半胱氨酸硅胶固定相SIL-AVI-Cys,并通过热重、红外光谱以及元素分析对材料进行了表征.填充SIL-AVI-Cys色谱柱在分离胞核嘧啶、胞苷、肌苷、尿嘧啶和尿苷等5种亲水性化合物时表现为亲水作用模式,而在分离苯胺和苯酚类物质时则可表现出亲水/反相混合模式特征.通过考察流动相pH值、盐浓度对分析物保留的影响,进一步探讨了该固定相的分离机理.流动相pH值的改变可通过改变分析物的离子化影响其与固定相之间的作用力及其保留行为.流动相中盐浓度的增加,可减弱酸性分析物的保留,而对于碱性和中性分析物则无显著影响.此外,SIL-AVI-Cys色谱柱的分离性能采用酰胺及核酸碱基/核苷类物质进行了表征,并将其进一步应用于奶粉中三聚氰胺的表征和检测.关键词:混合模式作用色谱;氨基酸离子液体;固定相;奶粉;三聚氰胺中图分类号:O657.7 文献标志码:A 文章编号:1000-1565(2020)01-0018-09Imidazolium-embedded cysteine-functionalized stationary phases: preparation and its chromatographic applicationsWANG Shengyu¹, LIANG Peng¹, DUAN Liguang², QIAO Xiaoqiang¹(1. College of Pharmacy, Hebei University, Baoding 071002, China; 2. Department of Pharmacy, The First Hospital of Hebei Medical University, Shijiazhuang 050000, China)Abstract: A novel imidazolium-embedded cysteine-functionalized stationary phase SIL-AVI-Cys was prepared by “click reaction”, which was further characterized by thermogravimetric analysis, FT-IR as well as elemental analysis. The stationary phase exhibits HILIC mode in the separation of five hydrophilic compounds, such as cytosine, cytidine, inosine, uracil and uridine, which also exhibits HILIC/RPLC mixed mode when separating anilines and phenolic substances. The separation mechanism of the stationary phase was further explored by investigating the effects of mobile phase pH and salt concentration on the retention of analytes. The change in the pH of the mobile phase can affect the interaction between analytes and the stationary phase by varying the dissociation of the analytes. The separation characteristics of the SIL-AVI-Cys column were also investigated by amides and nucleobases/nucleosides. The SIL-AVI-Cys column- 收稿日期:2019-04-23 基金项目:国家自然科学基金资助项目(21675039) 第一作者:王胜玉(1990—),男,河北霸州人,河北大学硕士研究生,主要从事色谱分析研究. E-mail: 983942430@qq.com 通信作者:段立广(1984—),男,河北邢台人,河北医科大学第一医院主管药师,主要从事药物新剂型相关研究. E-mail: dlg123456@163.com乔晓强(1982—),男,河北宁晋人,河北大学教授,博士,主要从事色谱分离分析新材料研究. E-mail: hbuqiao@163.com第1期王胜玉等:咪唑嵌合半胱氨酸修饰固定相制备及色谱应用was further used for characterization and detection of melamine in baby milk powder.

A fluorescent probe for detection of biothiols

ZHOU Keyan, YANG Yutao, LI huaxiao, LI Wei

2020, 40(1):  27-32.  DOI: 10.3969/j.issn.1000-1565.2020.01.005

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Biothiols, cysteine(Cys)and homocysteine(Hcy)and glutathione(GSH)play key roles in human body. Owing to the advantages of fluorescence probe method, such as high sensitivity, excellent selectivity, fast response time and the ability to detect thiols in vivo, a new fluorescence probe for the selective detection of biothiols based on the nucleophilic addition mechanism was designed and synthesized in this paper. Upon addition of biothiols, the fluorescence intensity increased with the increase of thiol concentration and reached its maximum within 2 min. The results showed that the probe has potential application in bioimaging.

Preparation and evaluation of doxorubicin loaded hyaluronic acid-pullulan microspheres in vitro and in vivo

YANG Wenzhi, ZHAO Yafei, WU Tong, LIU Jiaoyan, LI Haiying

2020, 40(1):  33-40.  DOI: 10.3969/j.issn.1000-1565.2020.01.006

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The natural macromolecular materials of pullulan(Pu)and hyaluronic acid(HA)have good biocompatibility and are often used as drug carriers. However, the rapid degradation of these natural macromolecule limits their application as drug sustained-release functional carriers. In this paper, the self-made anti-enzymatic degradation material of hyaluronic acid grafted pullulan(HA-Pu)is used to prepare its microspheres. In this system, HA-Pu material solution is used as the water phase, liquid paraffin as the oil phase, the Span 80 as the emulsifier, and the glutaraldehyde as the crosslinking agent to prepare the HA-Pu MPs. Box-Behnken Design(BBD)method was used to investigate the effects of rotational speed, oil-water ratio and HA-Pu concentration on the particle size of HA-Pu microspheres. When the optimal formulation conditions was selected as 670 r/min stirring speed, 5.6∶1(V/V, mL/mL)oil-water ratio and 4.48 g/mL HA-Pu, the microparticles had a smooth surface with about 18 μm diameter. The infrared spectrum- DOI:10.3969/j.issn.1000-1565.2020.01.006透明质酸基普鲁兰糖载药微球制备与体内外评价杨文智,赵亚非,吴桐,刘娇艳,李海鹰(河北大学 药学院,河北 保定 071002)摘 要:普鲁兰糖(Pu)和透明质酸(HA)天然高分子材料具备良好生物相容性,常用作药物载体,但此类天然高分子降解快,限制其作为药物的缓释功能载体的应用.本文拟采用自制抗酶降解的透明质酸接枝普鲁兰糖(HA-Pu)材料溶液为水相,液体石蜡为油相,Span 80为乳化剂,戊二醛为交联剂,利用乳化交联法制备HA-Pu微球(HA-Pu MPs). 利用Box-Behnken Design(BBD)法,考察转速、油水比和HA-Pu质量浓度等3因素对微球粒径的影响,当选择670 r/min搅拌转速,5.6∶1(体积比)油水比和44.8 mg/mL HA-Pu的最佳制备条件,可获得形态圆整且平均粒径约为18 μm的微球. 样品红外图谱显示,制备微球成功交联. 以阿霉素(DOX)为模型药,交联量戊二醛0.5 mol,最佳DOX与HA-Pu投料比为2∶10(质量比),获得载药量为5.02%(质量分数),包封率为33%的(载药微球)DOX-HA-Pu MPs. 载药微球体外释药曲线拟合符合Ritger-Peppas方程. 而对比大鼠尾静脉注射DOX药物和腹腔注射DOX-HA-Pu MPs,载药微球具备缓释功能. 利用HA-Pu材料抗酶降解性质,采用乳化交联法制备HA-Pu MPs,方法简单易行,制备微球有望成为抗瘤药物的缓释载体.关键词:微球;透明质酸;普鲁兰糖;盐酸阿霉素;体内外中图分类号:O63 文献标志码:A 文章编号:1000-1565(2020)01-0033-08Preparation and evaluation of doxorubicin loaded hyaluronic acid-pullulan microspheres in vitro and in vivo YANG Wenzhi, ZHAO Yafei, WU Tong, LIU Jiaoyan, LI Haiying(College of Pharmacy,Hebei University,Baoding 071002,China)Abstract: The natural macromolecular materials of pullulan(Pu)and hyaluronic acid(HA)have good biocompatibility and are often used as drug carriers. However, the rapid degradation of these natural macromolecule limits their application as drug sustained-release functional carriers. In this paper, the self-made anti-enzymatic degradation material of hyaluronic acid grafted pullulan(HA-Pu)is used to prepare its microspheres. In this system, HA-Pu material solution is used as the water phase, liquid paraffin as the oil phase, the Span 80 as the emulsifier, and the glutaraldehyde as the crosslinking agent to prepare the HA-Pu MPs. Box-Behnken Design(BBD)method was used to investigate the effects of rotational speed, oil-water ratio and HA-Pu concentration on the particle size of HA-Pu microspheres. When the optimal formulation conditions was selected as 670 r/min stirring speed, 5.6∶1(V/V, mL/mL)oil-water ratio and 4.48 g/mL HA-Pu, the microparticles had a smooth surface with about 18 μm diameter. The infrared spectrum- 收稿日期:2018-10-20 基金项目:河北省自然科学基金资助项目(H2017201052;H2018201045);河北大学研究生创新项目(hbu2019ss034);河北省高等学校科学技术项目(ZD2016102;ZD2018054) 第一作者:杨文智(1972—),男,内蒙古锡林浩特人,河北大学副教授,从事生物医用材料及药物缓控释制剂方面研究.第1期杨文智等:透明质酸基普鲁兰糖载药微球制备与体内外评价of the sample shows that the prepared microspheres are successfully crosslinked. By using doxorubicin(DOX)as a model drug, with the cross-linking amount of glutaraldehyde being 0.5 mol, and with the optimum drug loading mass ratio being 2∶10, DOX-HA-Pu MPs with 5% drug loading and 33% encapsulation efficiency were obtained. In vitro release curve of drug-loaded microspheres was consistent with the Ritger-Peppas equation. when DOX drugs were injected into tail vein of rats and DOX-HA-Pu MPs were injected intraperitoneally in rats, the drug-loaded microspheres have displayed a sustained-release function. In summary utilizing HA-Pu material anti-enzymatic degradation properties, HA-Pu MPs are prepared by emulsion cross-linking method which is simple and easy to operate. Therefore, the preparation of HA-Pu MPs is expected to be a sustained-release carrier of antineoplastic drugs.

Preparation of polymer containing p-phenyldiacetonitrile units with aggregation induced emission effect by one-pot method

LIANG Lixun, WANG Ying, GAO Luyao, CHEN Weiping, ZHAO Hongchi

2020, 40(1):  41-48.  DOI: 10.3969/j.issn.1000-1565.2020.01.007

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Compound α,α’-bis-(p-hydroxybenzylidene)-p-benzenediacetonitrile(M1)was synthesized by Knoevenagel condensation reaction using p-benzenediacetonitrile and p-hydroxybenzaldehyde as raw materials. Compound α,α’-bis-［p-(2,3-epoxypropoxy)-benzylidene］-p-benzenediacetonitrile(M2)was synthesized from M1 and epichlorohydrin by Williamson reaction. Polymer P1 was synthesized by using 2-(2',3',4',6'-tetra-O-acetyl-α-D-mannopyranosyloxy)ethyl methacrylate(M4)as monomer and 2-cyano-2-propyl benzodithioate as reversible addition-fragmentation transfer agent. Polymer P2 with defined structure was synthesized via one-pot reaction with P1 and M2(the reduction reaction of dithioester(P1)and click reaction between the thiol group and epoxy group(M2)were simultaneously conducted in one pot with high conversion). The water-soluble fluorescent polymer P3 was obtained in a high yield after the deacetylation protection reaction of P2. The chemical structures and optical properties of compounds and polymers were characterized and tested by nuclear magnetic resonance spectrum, Fourier transform infrared- DOI:10.3969/j.issn.1000-1565.2020.01.007一锅法制备具有AIE效应含对苯二乙腈单元聚合物梁立勋,王颖,高路谣,陈卫平,赵洪池(河北大学 化学与环境科学学院,河北 保定 071002)摘 要:以对苯二乙腈和对羟基苯甲醛为原料通过Knoevenagel缩合反应合成α,α’-双-(对羟基亚苄基)-对苯二乙腈(M1),然后与环氧氯丙烷通过Williamson反应合成α,α’-双-［对-(2,3-环氧丙氧基)亚苄基］-对苯二乙腈(M2). 以2-氰基-2-丙基苯并二硫为可逆加成断裂链转移试剂,引发2-(2',3',4',6'-四-O-乙酰基-α-D-吡喃甘露糖基氧基)甲基丙烯酸乙酯聚合制备了含双硫酯片段的聚合物P1,P1与M2通过“一锅法”合成含对苯二乙腈单元聚合物P2,脱除乙酰基保护得到水溶性荧光聚合物P3. 利用核磁共振波谱、傅里叶变换红外光谱、凝胶渗透色谱、紫外-可见光谱和荧光光谱对化合物和聚合物的化学结构和光学性质等进行了表征与测试. 结果表明:P3的紫外吸收峰和荧光发射峰分别在368 nm和440 nm,P3荧光强度随着DMSO-H₂O体系DMSO体积分数的增加而逐渐增强,并且在体积分数大于80%时荧光强度显著增强,表现出聚集诱导发光特性. P3具有较好的热稳定性,相转变温度为118 ℃.关键词:聚集诱导发光;可逆加成-断裂链转移;一锅法;点击化学中图分类号:O636 文献标志码:A 文章编号:1000-1565(2020)01-0041-08Preparation of polymer containing p-phenyldiacetonitrile units with aggregation induced emission effect by one-pot methodLIANG Lixun, WANG Ying, GAO Luyao, CHEN Weiping, ZHAO Hongchi(College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China)Abstract: Compound α,α’-bis-(p-hydroxybenzylidene)-p-benzenediacetonitrile(M1)was synthesized by Knoevenagel condensation reaction using p-benzenediacetonitrile and p-hydroxybenzaldehyde as raw materials. Compound α,α’-bis-［p-(2,3-epoxypropoxy)-benzylidene］-p-benzenediacetonitrile(M2)was synthesized from M1 and epichlorohydrin by Williamson reaction. Polymer P1 was synthesized by using 2-(2',3',4',6'-tetra-O-acetyl-α-D-mannopyranosyloxy)ethyl methacrylate(M4)as monomer and 2-cyano-2-propyl benzodithioate as reversible addition-fragmentation transfer agent. Polymer P2 with defined structure was synthesized via one-pot reaction with P1 and M2(the reduction reaction of dithioester(P1)and click reaction between the thiol group and epoxy group(M2)were simultaneously conducted in one pot with high conversion). The water-soluble fluorescent polymer P3 was obtained in a high yield after the deacetylation protection reaction of P2. The chemical structures and optical properties of compounds and polymers were characterized and tested by nuclear magnetic resonance spectrum, Fourier transform infrared- 收稿日期:2019-06-21 基金项目:河北省自然科学基金资助项目(B2018201281);河北大学研究生创新项目(hbu2018ss22) 第一作者:梁立勋(1994—),男,河北灵寿人,河北大学在读硕士研究生.E-mail:llx754931484@163.com 通信作者:赵洪池(1973—),男,河北定兴人,河北大学教授,博士,主要从事高分子化合物的合成及功能化研究. E-mail: zhc@hbu.edu.cn第1期梁立勋等:一锅法制备具有AIE效应含对苯二乙腈单元聚合物spectroscopy, gel permeation chromatography,ultraviolet-visible spectroscopy and fluorescence spectroscopy, These results showed that the ultraviolet-visible absorption peak and the fluorescence emission peak of P3 were 368 nm and 440 nm, Fluorescence intensity of P3 gradually increased with the increase of DMSO fraction in DMSO/H₂O system, when DMSO fraction was over 80%, which indicated the aggregation-induced emission characteristic of P3. Polymer P3 had good thermal stability, and the phase transition temperature was 118 ℃.

Advances in classification of the storage tenebrionid beetles in the world

REN Guodong,LI Xue

2020, 40(1):  49-56.  DOI: 10.3969/j.issn.1000-1565.2020.01.008

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The tenebrionid beetles that harm the stored goods directly or indirectly are closely associated with human economic interests, and are the most important part in the integrated pest management. With 277 species(subspecies)of 98 genera of 9 subfamilies known worldwide, this group accounts for 1.11% of the total species of the family Tenebrionidae. To date, 72 species(subspecies)of 32 genera of 5 subfamilies have been recorded from China. Being capable of thriving in the relatively dry storage environment,this special beetle group has adapted to the warehouse and bedroom habitats. Based on a large number of literatures, this paper summarizes the taxonomic, species diversity, distribution and occurrence of pests around the world. The main problems and shortcomings in taxonomic and the concerns for future research are preliminarily discussed, which can provide a guidance for the research and production management of storage pests in China.

Changes of Th1 and Th2 cytokine levels in uterus of abortive mice induced by Listeria monocytogenes

LI Wenyan, ZHANG Na, LI Xiaoning, LIU Chunfu, LIANG Ziteng, ZHONG Fei, GUAN Yueqiang

2020, 40(1):  57-62.  DOI: 10.3969/j.issn.1000-1565.2020.01.009

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To explore whether Th1 and Th2 cytokines play important roles in Listeria monocytogenes(LM)-induced mouse abortion,the pregnant mice and mouse macrophages were infected with LM. Embryo survival rates were calculated. Th1 and Th2 cytokine levels were analyzed in uterus and medium of mouse macrophages. Results showed that Th1 cytokine levels increased significantly in uterus of abortive mice induced by LM. Embryo survival rates and Th1 and Th2 cytokine levels showed no changes in uterus of mice infected by listeriolysin O(LLO)-deficient LM(Δ hly LM). Th1 cytokine and interleukin(IL)-4 levels increased- DOI:10.3969/j.issn.1000-1565.2020.01.009单增李斯特菌致流产小鼠子宫中Th1和Th2细胞因子的变化 李文艳^1,2,张娜³, 李晓宁¹,刘纯甫¹,梁子腾⁴,仲飞²,管越强³(1.河北大学 基础医学院,河北 保定 071002; 2. 河北农业大学 动物医学院,河北 保定 071000;3.河北大学 生命科学学院,河北 保定 071002;4.河北大学 公共卫生学院,河北 保定 071002)摘 要:为探讨Th1和Th2细胞因子在单增李斯特菌(Listeria monocytogenes,LM)致小鼠流产中的作用,用LM感染妊娠小鼠和小鼠巨噬细胞,统计胚胎存活率,酶联免疫测定(enzyme linked lmmuno sorbent assay,ELISA)小鼠子宫组织中和巨噬细胞培养基中主要的Th1和Th2细胞因子水平.结果显示,LM诱导的流产小鼠子宫中Th1细胞因子水平显著增加,溶血素O(listeriolysin O,LLO)基因缺陷型(Δ hly)LM感染组胚胎存活率和子宫中Th1、Th2细胞因子水平无显著变化;LM感染的巨噬细胞分泌的Th1细胞因子和白细胞介素-4(interleukin, IL-4)水平显著增加,IL-10水平显著降低,Δ hly LM感染组Th1和Th2细胞因子水平无显著变化.因此,LM感染后子宫中Th1/Th2细胞因子失衡诱导了小鼠流产,这可为临床防治LM诱导的流产提供科学依据.关键词:单增李斯特菌;流产;Th1和Th2细胞因子;巨噬细胞中图分类号:S855.1 文献标志码:A 文章编号:1000-1565(2020)01-0057-06Changes of Th1 and Th2 cytokine levels in uterus of abortive miceinduced by Listeria monocytogenes LI Wenyan^1,2, ZHANG Na³, LI Xiaoning¹, LIU Chunfu¹, LIANG Ziteng⁴, ZHONG Fei², GUAN Yueqiang³(1. College of Basic Medicine, Hebei University,Baoding 071002, China; 2. College of Veterinary Medicine, Agriculture University of Hebei,Baoding 071000, China; 3. College of Life Sciences, Hebei University,Baoding 071002, China; 4.College of Public Health, Hebei University,Baoding 071002, China)Abstract: To explore whether Th1 and Th2 cytokines play important roles in Listeria monocytogenes(LM)-induced mouse abortion,the pregnant mice and mouse macrophages were infected with LM. Embryo survival rates were calculated. Th1 and Th2 cytokine levels were analyzed in uterus and medium of mouse macrophages. Results showed that Th1 cytokine levels increased significantly in uterus of abortive mice induced by LM. Embryo survival rates and Th1 and Th2 cytokine levels showed no changes in uterus of mice infected by listeriolysin O(LLO)-deficient LM(Δ hly LM). Th1 cytokine and interleukin(IL)-4 levels increased- 收稿日期:2019-04-01 基金项目:河北省自然科学基金资助项目(C2017201213);河北省现代农业产业技术体系淡水养殖创新团队项目(HBCT2018180203);河北大学中西部高校提升综合实力专项基金资助项目 第一作者:李文艳(1973— ),女,河北清苑人,河北大学副教授,博士,主要从事生殖生物学与分子免疫学研究.E-mail: liwenyan358@163.com 通信作者: 仲飞(1956—),男,河北蔚县人,河北农业大学教授,博士,主要从事分子病毒与分子免疫学研究. E-mail: feizhong2013@126.com第1期李文艳等:单增李斯特菌致流产小鼠子宫中Th1和Th2细胞因子的变化 while IL-10 level decreased in the medium of macrophages infected by wild-type LM. After infected by Δ hly LM, Th1 and Th2 cytokine levels showed no changes. Therefore, LM-induced Th1/Th2 imbalance in uterus can causes the mouse abortion,which provides scientific basis for clinical prevention and treatment of LM-induced abortion.

Signal molecule produced by Phytophthora infestans induced actinomyces Sy11

HAO Zhiwei, JIANG Jizhi, WU Lufang, ZHANG Hongxia, ZHANG Hehua

2020, 40(1):  63-70.  DOI: 10.3969/j.issn.1000-1565.2020.01.010

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Phytophthora infestans could produce some signal molecules which stimulated or induced Streptomyces microflavus Sy11 strain to produce anti-oomycetes substances when it was cultured against the S. microflavus Sy11 strain. To clarify in which culture mode P. infestans could more likely produce these signal molecules and what kinds of substance they are, the growth rate method was adopted to compare the induction activities of the signal molecules produced by the pathogen between individual culture(solid culture and liquid culture)and dual culture(solid culture and liquid culture)with S. microflavus Sy11 strain, and different organic solvents were used to analyze the substance type of the signal moleculars. The results showed- DOI:10.3969/j.issn.1000-1565.2020.01.010对放线菌Sy11有诱导作用的致病疫霉信号分子研究郝志伟,蒋继志,吴路芳,张红霞,张荷花(河北大学 生命科学学院,河北 保定 071002)摘 要:致病疫霉(Phytophthora infestans)能产生一种信号分子,诱导放线菌Sy11菌株产生抑菌物质从而抑制致病疫霉.为明确在哪种培养方式下致病疫霉更易产生这种信号分子以及信号分子的物质类别,本文采用生长速率法对致病疫霉单独培养(单独固体培养和单独液体培养)以及与Sy11共培养(对峙培养和混合培养)产生的信号分子诱导Sy11抑菌作用的活性进行了比较,并以不同有机溶剂对信号分子的物质类别进行了初步分析.结果显示,致病疫霉无论单独培养还是与Sy11共培养,均可产生诱导Sy11抑菌作用的信号分子,其中以在固体黑麦培养基上与Sy11对峙培养产生的信号分子的诱导活性最强,菌落直径为24.79 mm,但与其他3种培养方式所产生的信号分子的诱导活性并无显著性差异;对致病疫霉单独液体培养后无菌体发酵液的分析表明,其中脂类粗提物的诱导活性(菌落直径32.8 mm)远高于糖类(菌落直径61.5 mm)和蛋白质类(菌落直径60 mm)粗提物,进一步对其中脂类物质的萃取显示,乙酸乙酯的萃取效果明显优于乙醇和石油醚.乙酸乙酯萃取物诱导Sy11后对致病疫霉菌丝生长有显著的抑制作用(菌落直径为35 mm)与乙醇萃取液的处理达到显著性差异.以上结果表明,致病疫霉生长过程中无论单独培养还是与Sy11共培养均可产生能诱导Sy11抑菌作用的信号分子,该信号分子以脂类物质为主,可用乙酸乙酯萃取得到.关键词:致病疫霉;Sy11菌株;诱导感应现象;信号分子中图分类号:S435.32 文献标志码:A 文章编号:1000-1565(2020)01-0063-08Signal molecule produced by Phytophthora infestans induced actinomyces Sy11HAO Zhiwei, JIANG Jizhi, WU Lufang, ZHANG Hongxia, ZHANG Hehua(College of Life Sciences, Hebei University, Baoding 071002,China)Abstract: Phytophthora infestans could produce some signal molecules which stimulated or induced Streptomyces microflavus Sy11 strain to produce anti-oomycetes substances when it was cultured against the S. microflavus Sy11 strain. To clarify in which culture mode P. infestans could more likely produce these signal molecules and what kinds of substance they are, the growth rate method was adopted to compare the induction activities of the signal molecules produced by the pathogen between individual culture(solid culture and liquid culture)and dual culture(solid culture and liquid culture)with S. microflavus Sy11 strain, and different organic solvents were used to analyze the substance type of the signal moleculars. The results showed- 收稿日期:2019-05-31 基金项目:河北省自然科学基金资助项目(C2015201231);河北省研究生创新项目(CXZZSS2018002) 第一作者:郝志伟(1993—),男,河北大学在读硕士研究生,主要从事植物与微生物相互作用的分子机理研究.E-mail:2942236040@qq.com 通信作者:蒋继志(1960—),男,河北大学教授,博士生导师,主要从事植物病害生物防治及其分子机理研究.E-mail:jizhijiang909@163.com第1期郝志伟等:对放线菌Sy11有诱导作用的致病疫霉信号分子研究that P.infestans could produce signal molecules inducing Sy11 either alone or in co-culture with Sy11. In which, the most induction activity was found in co-cultured of P.infestans with Sy11 on solid rye medium, the colony diameter was 24.79 mm, but there were no significant differences comparing with other three culture modes. Analysis of free cell fermentation broth of P.infestans culture alone by different organic solvents showed that induction activity of crude lipid extracts or lipid-containing substances(colony diameter 32.8 mm)was much higher than that of polysaccharide crude extracts(colony diameter 61.5 mm)and protein crude extracts(colony diameter 60 mm). Futher experimental results indicated that the extraction effect of the ethyl acetate was better than that of ethanol and petroleum ether. The colony diameter of P.infestans was 35 mm after induction by the ethyl acetate extracts and the significant difference was found between ethyl acetate and ethanol extracts treatments. These findings suggested that P.infestans could produce some signal molecules which induce the anti-oomycetes of Sy11 strain in both individual culture and co-culture mode with Sy11, these signal molecules are lipid substances and easily extracted with ethyl acetate.

Fermentation method and related detection of Auricularia auricula under the mechanism of extracting the spike of cattail

PING Linlin,WU Yánan,KOU Boxiang,WANG Qian

2020, 40(1):  71-76.  DOI: 10.3969/j.issn.1000-1565.2020.01.011

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Through the single factor test and the orthogonal test method, the optimal carbon and nitrogen source and the optimal formula for the fermentation of Auricularia auricula the mechanism of extracting the spike of cattail were studied. The fermentation broth was used to homogenize the fermentation broth for the best bacterial age, and was used to culture the test drosophila, and the drosophila life test was carried out to observe the effect on the life span of the drosophila. The results showed that the optimal fermentation formula was: glucose 3%, corn flour 3%, bean cake powder 2%, yeast extract 0.5%, potassium phosphate monobasic 0.1%, magnesium sulfate 0.1%; and when fermented to 120 h, the wet weight of mycelium reached the maximum value of 32.57 g. When 30% fermented mash was added to the drosophila basal medium, the half-life death rate, average life expectancy and maximum life-long life rate of drosophila were the highest, and they were significantly different from the control group(P<0.05)

An improved collaborative filtering recommendation algorithm

LI Kunlun, RONG Jingyue,SU Huading

2020, 40(1):  77-86.  DOI: 10.3969/j.issn.1000-1565.2020.01.012

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The collaborative filtering recommendation algorithm is one of the most widely used algorithms in the personalized recommendation system, but it also faces problems such as data sparsity, cold start, and scalability. This paper mainly proposes an improved data filling method and similarity calculation method for the inaccurate recommendation effect caused by the data sparsity problem and the cold start problem. Firstly, the user is hierarchically clustered according to the user's scoring habits, and then the users basic information such as age is used to calculate the similarity between users, and the ratio of the common scoring items is used as the weight to obtain the user similarity. Finally, the Slope-one algorithm is used to calculate the padding values of the first K similar users, and the similarity weights are added to obtain the final padding value. When calculating similarity to find the nearest neighbor set,the basic attribute of the user is used as the similarity weight,and the Sigmoid function is introduced to add the impact of the timestamp on the similarity and obtain the final similarity calculation method The experimental results show that the recommendation accuracy is significantly improved, and at the same time the data sparsity problem and the cold start problem are improved.

Optimal terminal configuration method based on recursive search and genetic algorithms

ZHANG Qiang,YANG Yunjie,ZHAO Miao,HUO Limin,TANG Wei

2020, 40(1):  87-94.  DOI: 10.3969/j.issn.1000-1565.2020.01.013

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A terminal configuration optimization method based on the combination of depth-first search and genetic algorithm is proposed. This method uses each load point as the starting point to search the main circuit and sub-circuit in the distribution network with distribution terminals. The search simultaneously judges the influence of current faults on the reliability of power supply at load points and accumulates. By constructing the selection conditions of selection operators, the depth-first search can be carried out. The combination of reliability calculation method and genetic algorithm can solve the optimization problem of distribution terminals quickly, and can be easily programmed on computer. It can also solve the optimal terminal configuration of different distribution networks on the premise of modifying only the basic parameters of the network. The calculation example takes different average power supply availability as constraints, and analyses the terminal configuration scheme and economic benefits under different constraints, which verifies the effectiveness of the proposed algorithm.

Health assessment of photovoltaic modules based on fuzzy theory and combined weights

JIA Ling,HUANG Lihua,LIU Xuefei,HAN Jinglin,TANG Wei

2020, 40(1):  95-103.  DOI: 10.3969/j.issn.1000-1565.2020.01.014

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Photovoltaic energy has been widely used for its energy regeneration and clean nature, and has broad development prospects. However, the health status during its operation directly affects the safety of power system operation, power quality and economy. Therefore, it is necessary to evaluate the health status of PV(photovoltaic)modules to achieve the purpose of ‘early detection and early treatment’. In this paper, fuzzy theory, cloud model, analytic hierarchy process and entropy weight method are used to establish the health assessment model of PV modules. Taking the operating parameters, environmental parameters and patrol parameters as the main basis, considering the multi-level and multi-factor of the PV module status information, the complex problems are decomposed and a hierarchical index system for PV module status assessment is established. According to the analytic hierarchy process(AHP)and the entropy weight method, - DOI:10.3969/j.issn.1000-1565.2020.01.014基于模糊理论和组合权重的光伏组件健康状况评估贾玲¹,黄丽华¹,刘雪飞²,韩璟琳²,唐巍³(1. 河北农业大学 机电工程学院,河北 保定 071000;2.国网河北省电力有限公司 经济技术研究院,河北 石家庄 050021;3.中国农业大学 信息与电气工程学院,北京 100083)摘 要:光伏能源以其能源再生和清洁为特点得到广泛应用,并具有广阔发展前景,然而其运行过程中的健康状况直接影响电力系统运行的安全性、经济性和电能质量,因此需要对光伏组件进行健康状况评估,以达到“早发现,早治疗”的目的.综合运用模糊理论、云模型及层次分析法和熵权法建立了光伏组件健康状况评估模型.以运行参数、环境参数、巡视参数为主要依据,考虑到光伏组件状况的多层次性和多因素性,进而将复杂问题进行分解,建立了光伏组件状况评估的层次指标体系.根据层次分析法(AHP)和熵权法确定权重向量模型,利用云模型建立模糊关系,依此建立光伏组件健康状况评估模型,对层次指标体系中的各层次的权重向量模型和模糊关系模型进行模糊运算,最终得到光伏组件健康状况的隶属度.实例分析表明,该方法是可行有效的.关键词:光伏组件;健康评估;模糊理论;云模型;组合权重中图分类号:TM 615 文献标志码:A 文章编号:1000-1565(2020)01-0095-09Health assessment of photovoltaic modules based on fuzzy theory and combined weightsJIA Ling¹,HUANG Lihua¹,LIU Xuefei²,HAN Jinglin²,TANG Wei³(1.College of Mechanical and Electrical Engineering,Agricultural University of Hebei,Baoding 071000,China;2. Economic Research Institute,State Grid Hebei Electric Power Co. Ltd.,Shijiazhuang 050021,China;3.School of Information and Electrical Engineering, China Agricultural University,Beijing 100083,China)Abstract: Photovoltaic energy has been widely used for its energy regeneration and clean nature, and has broad development prospects. However, the health status during its operation directly affects the safety of power system operation, power quality and economy. Therefore, it is necessary to evaluate the health status of PV(photovoltaic)modules to achieve the purpose of ‘early detection and early treatment’. In this paper, fuzzy theory, cloud model, analytic hierarchy process and entropy weight method are used to establish the health assessment model of PV modules. Taking the operating parameters, environmental parameters and patrol parameters as the main basis, considering the multi-level and multi-factor of the PV module status information, the complex problems are decomposed and a hierarchical index system for PV module status assessment is established. According to the analytic hierarchy process(AHP)and the entropy weight method, - 收稿日期:2019-07-07 项目基金:国家自然科学基金资助项目(51377162);国网河北省电力有限公司项目(SGHEJY00GHJS1900028) 第一作者:贾玲(1994—),女,河北保定人,河北农业大学在读硕士研究生.E-mail:876535449@qq.com 通信作者:黄丽华(1963—),女,山东荣成人,河北农业大学教授,博士,主要从事配电系统及其自动化、人工智能方面的研究. E-mial:962382758@163.com第1期贾玲等:基于模糊理论和组合权重的光伏组件健康状况评估the weight vector model is determined, and the cloud model is used to establish the fuzzy relationship. According to the health status of the PV module, the condition evaluation model is established, and the weight vector model and the fuzzy relationship model of each level in the hierarchical index system are established. The fuzzy operation is performed to finally obtain the membership degree of the health status of the photovoltaic module. Case analysis shows that the method is feasible and effective.

Application of new PSGA algorithm in performance optimization of general aviation logistics system

ZHANG Hao, AN Jingwen

2020, 40(1):  104-112.  DOI: 10.3969/j.issn.1000-1565.2020.01.015

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In order to enhance the performance of the general aviation logistics system which requires mach higher time efficiency, this paper designs and proposes a new variable population and pole search genetic algorithm(PSGA). By redesigning the traditional genetic algorithm(GA)in the logical structure, and innovatively designing and introducing a fitness factor, the algorithm efficiency of PSGA is made higher than that of traditional GA.Through two function optimization tests with different complexity,the efficiency of PSGA is made higher than that of GA for 35.35% and 43.50%. Finally, the practical application shows that the convergence efficiency of PSGA is higher than that of GA for 25 generations and the optimization accuracy is higher than that of GA for 1.46. The tests and application results show that PSGA algorithm has better effectiveness and applicability in the performance optimization of general aviation logistics system.