Table of Content

25 November 2019, Volume 39 Issue 6

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A TODIM decision making method in single-valued neutrosophic linguistic environment

GUO Zixue, SUN Fangfang

2019, 39(6):  561-567.  DOI: 10.3969/j.issn.1000-1565.2019.06.001

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Based on the uncertainty of attribute value information and the different risk attitudes of decision-makers towards gain and loss, an extended TODIM(an acronym in Portuguese of interactive and multi-criteria decision making)under single-valued neutrosophic linguistic environment is proposed. First, the uncertainty in attribute value information can be quantified directly by using single-valued neutrosophic language set. Second, based on the extended language scale function, the distance measure and aggregator of single-valued neutrosophic linguistic numbers are defined. Based on these, weights are calculated by standard deviation and ranks are obtained by the TODIM method. Finally, the practical application process of the proposed method is illustrated by the case of energy-saving service company selection, which verifies the feasibility and effectiveness of the method.

Generalization of Holder's inequality and it's applications

HUANG Miaomiao, WANG Fengli, GAO Hongya

2019, 39(6):  568-572.  DOI: 10.3969/j.issn.1000-1565.2019.06.002

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A generalization of the classical Hölders inequality is given, which is applied to the study the boundary value problems of nonlinear divergence type elliptic equation when the boundary value is not zero. The regularity property of the solution is obtained by using Stampacchia lemma and the technique of Sobolev spaces.

Stress deformation and simulation analysis of semi-rigid rubber asphalt pavement under different wheel pressure and thickness

YANG Sanqiang,HE Wei,WU Haonan,CAI Yongli

2019, 39(6):  573-578.  DOI: 10.3969/j.issn.1000-1565.2019.06.003

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With the implementation of the urban construction plan of the Xiongan New District and according to the construction standards that the road required no minor in Xiongan New District in 15 years,Hebei province has launched an important project for the development and testing of major equipment for the accelerated loading test of the whole road.The global pavement accelerated loading test device was used to test the performance of the semi-rigid rubber asphalt pavement structure.It combinedwith mechanical- DOI:10.3969/j.issn.1000-1565.2019.06.003不同轮压与厚度下的半刚性橡胶沥青路面结构受力变形与仿真分析杨三强¹,何伟¹,吴浩楠²,蔡永利³(1. 河北大学 建筑工程学院,河北省土木工程监测与评估技术创新中心,河北 保定 071002;2.河北建设集团股份有限公司 路桥公司,河北 保定 071070;3.中电建冀交高速公路投资发展有限公司,河北 石家庄 050051)摘 要:随着国家雄安新区城市建设规划实施,按照雄安新区道路建设15年不小修的建设标准,河北省启动了全域路面加速加载试验装置开发与试验路评价研究专项.本研究采用全域路面加速加载试验装置测试直道半刚性橡胶沥青路面结构性能的各项指标,结合力学分析法与有限元建模计算分析层底弯拉应力与层厚.通过构建有限元模型,分析不同轮压下(0.7、0.9、1.1 MPa)的半刚性橡胶沥青路面各结构层间的应力应变变化规律及相同结构层不同轮压下的受力变形规律.结果表明,沥青路面作为粘弹性体,路面结构受力同时遵循能量耗散原理,自上而下逐渐减小.提出了半刚性胶粉改性沥青路面结构层在不同轮压、不同深度下的应力变化规律,得出了半刚性胶粉改性沥青路面各层在不同荷载下的应力值变化范围与半刚性水泥稳定碎石基层在不同荷载下的应力值变化范围.这些研究成果对今后雄安新区耐久性沥青路面建设起到了重要的理论支撑与技术指导价值.关键词:轮压;半刚性路面结构;橡胶沥青;受力变形;数值仿真分析 中图分类号:U416.217 文献标志码:A 文章编号:1000-1565(2019)06-0573-06Stress deformation and simulation analysis of semi-rigid rubber asphalt pavement under different wheel pressure and thicknessYANG Sanqiang¹,HE Wei¹,WU Haonan²,CAI Yongli³(1.Hebei Civil Engineering Monitoring and Performance Evaluation Technology Innovation Center,College of Civil Engineering and Architecture, Hebei University, Baoding 071002, China;2.Rood and Bridge Brunch,Hebei Construction Group Corporation Limited,Baoding 071070,China;3.Zhong Dian Jian Ji Jiao Express Way Investment Development Co.Ltd.,Shijiazhuang 050051,China )Abstract: With the implementation of the urban construction plan of the Xiongan New District and according to the construction standards that the road required no minor in Xiongan New District in 15 years,Hebei province has launched an important project for the development and testing of major equipment for the accelerated loading test of the whole road.The global pavement accelerated loading test device was used to test the performance of the semi-rigid rubber asphalt pavement structure.It combinedwith mechanical- 收稿日期:2019-03-27 基金项目:河北省自然科学基金资助项目(E2018201106);河北省交通运输厅科技项目(TH-201925;TH-201918);河北省高层次人才资助项目(B2017005024);交通运输部科技示范项目(2016010) 第一作者:杨三强(1980—),男,四川绵阳人,河北大学教授,博士后,主要从事公路工程路基路面工程研究. E-mail:ysq0999@163.com第6期杨三强等:不同轮压与厚度下的半刚性橡胶沥青路面结构受力变形与仿真分析analysis and finite element modeling, the bending stress and layer thickness of the layer are analyzed.The finite element model was constructed to analyze the stress-strain variation between the structural layers of semi-rigid rubber asphalt pavement under different wheel pressures(0.7 MPa, 0.9 MPa, 1.1 MPa).And it also analyzes the force deformation law of structural layers under different wheel pressures of the same structural layer.The results show that the asphalt pavement acts as a viscoelastic body and the stress of the pavement structure follows the principle of energy dissipation.Stress changes gradually, decreasing from top to bottom.The variation range of stress value of different layers of semi-rigid rubber powder modified asphalt pavement under different loads and the variation range of stress value of semi-rigid cement stabilized macadam base under different loads are obtained in this study.At last,these research results will serve as an important theoretical support and technical guidance value for guiding the construction of durable asphalt pavement in Xiong'an New District in the future.

Multi-scale analysis of liquid film parameter in vertical gas-liquid annular upflow

ZHAO Ning,ZHANG Lin, NIU Lina, LIU Yuhang, ZHAO Zhiyue

2019, 39(6):  579-586.  DOI: 10.3969/j.issn.1000-1565.2019.06.004

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Annular flow is one of the most important flow regimes that commonly found in many industrial applications, especially petroleum and chemical industry, air conditioning and refrigeration system and nuclear reactor cooling etc. The time-series signals which are directly related to the thickness of the liquid film are studied by using the wavelet transform processing method and several typical recursive parameters from a multi-scale perspective.The reconstruction coefficients and power spectra of time-series signals - DOI:10.3969/j.issn.1000-1565.2019.06.004垂直上升气液环状流液膜参数多尺度分析赵宁¹,张琳^1,2,牛立娜³,刘雨航¹,赵治月^1,4(1.河北大学 质量技术监督学院,河北 保定 071002;2.天津大学 电气自动化与信息工程学院,天津 300072;3.河北省计量监督检测研究院 热工与能源计量研究所,河北 石家庄 050051;4.沧州师范学院 机械与电气工程学院, 河北 沧州 061001)摘 要:气液两相环状流是最常见、最典型的流型之一,广泛存在于工业应用领域,特别是在石油化工、空气调节冷却系统、核反应堆冷却等领域.利用近红外光吸收衰减技术对气液两相垂直上升环状流的重要流动参数——液膜厚度进行了测量,将与液膜厚度直接相关的光强时序信号利用小波变换的多尺度处理方法进行研究分析,获得其重构系数和功率谱;通过对采集到的时序信号的确定性和平均对角线长度2个递归参量进行了分析,得到了液膜表面扰动波的变化和入口参数之间的关系,为更好地认识液膜波动、演化提供了有效的技术手段.关键词:关键词:扰动波;液膜;小波分析;多尺度;递归分析中图分类号:O359;TK313 文献标志码:A 文章编号:1000-1565(2019)06-0579-08Multi-scale analysis of liquid film parameter in vertical gas-liquid annular upflow ZHAO Ning¹,ZHANG Lin^1,2, NIU Lina³, LIU Yuhang¹, ZHAO Zhiyue^1,4(1.College of Quality and Technical Supervision, Hebei University, Baoding 071002, China;2. School of Electrical and Information Engineering, Tianjin University, Tianjin 300072, China;3.Institute of Thermal and Energy Measurement, Measurement Supervision and Testing Institute of Hebei Province, Shijiazhuang 050051, China;4. College of Mechanical and Electrical Engineering, Cangzhou Normal University, Cangzhou 061001, China)Abstract: Annular flow is one of the most important flow regimes that commonly found in many industrial applications, especially petroleum and chemical industry, air conditioning and refrigeration system and nuclear reactor cooling etc. The time-series signals which are directly related to the thickness of the liquid film are studied by using the wavelet transform processing method and several typical recursive parameters from a multi-scale perspective.The reconstruction coefficients and power spectra of time-series signals - 收稿日期:2019-01-10 基金项目:国家自然科学基金资助项目(61475041);河北省高层次人才项目(B2018003003);河北省教育厅青年基金资助项目(QN2018046);河北大学高层次人才科研启动项目(521000981319);沧州市自然科学基金资助项目(187000003) 第一作者:赵宁(1983—),男,河北保定人,河北大学讲师,博士,主要从事多相流理论及参数检测技术研究.E-mail:zhaoning1983@tju.edu.cn 通信作者:赵治月(1983—),男,河北沧县人,沧州师范学院副教授,河北大学在读博士研究生,主要从事光电检测技术.E-mail:zzyue1123@163.com第6期赵宁等:垂直上升气液环状流液膜参数多尺度分析are obtained. By analyzing the two recursive parameters, the detorminism and the average diagonal line length, the relationship between the change of the liquid film thickness and the input parameters can be obtained. The paper provides an effective technological means for better understanding of membrane fluctuation and evolution.

First-principle study of magnetic shape memory effect of Mn₂RhZ(Z=In, Sn, Sb)Heusler alloy

ZHANG Miao, ZHANG Lianshui

2019, 39(6):  587-593.  DOI: 10.3969/j.issn.1000-1565.2019.06.005

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The electronic structure and magnetic structure optimization on cubic austenitic Mn₂RhZ(Z=In, Sn, Sb)alloy in Cu₂MnAl and the Hg₂CuTi type structures have been investigated by using first principle calculations. In the austenitic phase, the Mn₂RhZ(Z=In, Sn, Sb)alloy of Hg₂CuTi type structure is more stable. With the tetragonal distortion, the global energy minimum occurs at c/a=1.38, 1.29, 1.29. The total magnetic moments of Mn₂RhZ(Z=In,Sn,Sb)mainly determined by the anti-parallel aligned Mn(A)and Mn(B)atoms spin moments. The magnetic moments of two Mn atoms in the Mn₂RhSb alloy in the austenite phase exhibit anti-parallel coupling, while the Mn₂RhZ(Z=In, Sn)in austenite and martensite phases and the Mn₂RhSb alloy in martensite phase are ferrimagnetic. The Mn₂RhZ(Z=In, Sn, Sb)alloys are potential materials with significant magnetic shape memory effect.

Determination and profiling of seven phenolic acids in wines and beverages by capillary electrophoresis with dynamic pH junction

LI Meiting,CHEN Yue,LI Yaru,LI Huaidong,ZHOU Can,CHU Xiaoxue, ZHANG Hongyi

2019, 39(6):  594-604.  DOI: 10.3969/j.issn.1000-1565.2019.06.006

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A method of capillary electrophoresis combined with dynamic pH junction online concentration for determination and profiling of seven phenolic acids in wines and beverages was developed. The sample containing 7 phenolic acids was dissolved in the specific background electrolyte, phosphate buffer at pH 3.5, which was different from the background electrolyte in electrophoretic separation medium,20 mmol/L borate buffer containing 25% methanol(V/V)at pH 9.2. The experimental factors influencing online concentration and analysis were systematically studied, induding detection wavelength, fraction of methanol in running background electrolyte, applied voltage and injection time. Under optimized conditions, the correlation coefficients for the calibration lines of the 7 phenolic acids were greater than 0.999 2, with limits of detection(S/N=3)in the range of 0.014~0.027 μg/mL. The recoveries for the selected phenolic- DOI:10.3969/j.issn.1000-1565.2019.06.006动态pH连接-毛细管电泳法测定和表征饮品中7种酚酸李美婷,陈跃,李亚茹,李怀栋, 周灿,褚晓雪,张红医(河北大学 化学与环境科学学院,河北 保定 071002)摘 要:建立了一种用于测定和表征葡萄酒和饮料中7种酚酸的毛细管电泳结合动态pH在线富集的方法.将含有7种酚酸的样品溶解于pH为3.5的磷酸盐背景电解质中,它与作为电泳分离介质的背景电解质不同(pH 9.2的20 mmol/L硼酸缓冲液中含有体积分数25%的甲醇).系统地研究了影响在线富集和分析的各种实验因素(检测波长、运行背景电解液中甲醇的含量、分离电压和进样时间等).在最优条件下, 7种酚酸的工作曲线的相关系数均大于0.999 2,检出限LOD(S/N=3)为0.014~0.027 μg/mL,加标回收率在 80%~130%.样品制备步骤非常简单,只需要用酸性背景电解质对样品进行稀释和过滤,一个样品的分析(包括样品处理和电泳分析)在大约16 min内完成.这种方法成功应用于葡萄酒和果汁中酚酸的测定和表征.关键词:毛细管电泳;动态pH连接;酚酸;葡萄酒;饮料中图分类号:O625.1 文献标志码:A 文章编号:1000-1565(2019)06-0594-11Determination and profiling of seven phenolic acids in wines and beverages by capillary electrophoresis with dynamic pH junctionLI Meiting,CHEN Yue,LI Yaru,LI Huaidong,ZHOU Can,CHU Xiaoxue, ZHANG Hongyi(College of Chemistry and Environmental Science, Hebei University, Baoding 071002,China)Abstract: A method of capillary electrophoresis combined with dynamic pH junction online concentration for determination and profiling of seven phenolic acids in wines and beverages was developed. The sample containing 7 phenolic acids was dissolved in the specific background electrolyte, phosphate buffer at pH 3.5, which was different from the background electrolyte in electrophoretic separation medium,20 mmol/L borate buffer containing 25% methanol(V/V)at pH 9.2. The experimental factors influencing online concentration and analysis were systematically studied, induding detection wavelength, fraction of methanol in running background electrolyte, applied voltage and injection time. Under optimized conditions, the correlation coefficients for the calibration lines of the 7 phenolic acids were greater than 0.999 2, with limits of detection(S/N=3)in the range of 0.014~0.027 μg/mL. The recoveries for the selected phenolic- 收稿日期:2019-02-15 基金项目:教育部留学回国人员科研启动基金(47批);河北省高等学校科学技术研究重点项目(ZD2019089);河北大学2018年实验室开放项目(sy201801);河北大学2019国家级和校级大学生创新创业计划项目(201910075018;2019191);2020年度河北大学研究生创新资助项目 第一作者:李美婷(1993—),女,河北廊坊人,河北大学在读硕士研究生.E-mail:1535582479@qq.com 通信作者:张红医(1967—),男,河北唐山人,河北大学教授,主要从事色谱、样品前处理和识别分析研究.E-mail:freetime20100401@163.comacids were in the range of 80%~130%. The sample preparation step was so simple that only dilution with acidic background electrolyte as well as filtration were required, leading to the analysis time for one sample 16 min, including sample treatment and electrophoretic analysis.The developedmethod was applied to determination and profiling of 7 phenolic acids in wines and beverages.

Study on the extraction of ursolic acid and oleanolic acid of Prunella vulgaris by semi-bionic method

FAN Baorui, MA Fengbo, LIU Hui, QIU Mingheng, LI Haiying

2019, 39(6):  605-610.  DOI: 10.3969/j.issn.1000-1565.2019.06.007

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Pulverized prunella vulgaris was extracted with ethanol or water by semi-bionic method. The contents of ursolic acid(UA)and oleanolic acid(OA)were determined by high performance capillary electrophoresis(HPCE)and compared with those by the traditional ethanol extraction method. HPCE with UV detector at 210 nm was applied using a silica capillary column(50 μm×61 cm, effective length of 50 cm). The sample was injected with a pressure injection of 19.3 MPa for 3 s. The separation buffer was composed of 25 mmol/L sodium tetraborate and 1.5 mmol/L β-cyclodextrin buffer(pH 11). HPCE method was performed at room temperature and the runningvoltage was 14.3 kV. The results showed that the HPCE- DOI:10.3969/j.issn.1000-1565.2019.06.007半仿生法提取夏枯草中熊果酸和齐墩果酸范保瑞¹,马逢伯¹,刘卉¹,邱明恒²,李海鹰²(1.北京市平谷区食品药品安全监控中心,北京 101200;2.河北大学 药学院,河北 保定 071002)摘 要:分别以水和乙醇为提取溶剂,采用半仿生法提取夏枯草,高效毛细管电泳法(HPCE)测定夏枯草中熊果酸(ursolic acid,UA)和齐墩果酸(oleanolic acid,OA)的含量,并与传统的乙醇提取方法进行比较研究.HPCE法测定条件为:未涂层石英毛细管柱(50 μm i.d.×61 cm,有效长度50 cm);运行缓冲液为25 mmoL/L四硼酸钠(含1.5 mmoL/L的β-环糊精溶液,pH=11);运行电压为14.3 kV;氮气压进样,进样压力19.3 MPa,进样时间3 s;紫外检测波长为210 nm;室温.结果表明:采用HPCE法分析测定夏枯草中UA和OA含量,方法的精密度、准确度良好.传统乙醇提取法中UA和OA质量分数分别为4.116 mg/g和0.700 mg/g,半仿生法乙醇提取液中2者质量分数分别为4.802 mg/g和0.975 mg/g,分别是传统乙醇提取法的1.17倍和1.39倍,而水提取液中2者质量分数较低.半仿生法采用pH 2.0、pH 7.5和pH 8.3的提取液分别提取中药材40、40和60 min,以符合人体胃肠消化转运规律,经实验验证将该法运用于夏枯草中2种三萜酸成分的提取可行.关键词:半仿生提取法;高效毛细管电泳法;夏枯草;熊果酸;齐墩果酸中图分类号:O65 文献标志码:A 文章编号:1000-1565(2019)06-0605-06Study on the extraction of ursolic acid and oleanolic acid of Prunella vulgaris by semi-bionic methodFAN Baorui¹, MA Fengbo¹, LIU Hui¹, QIU Mingheng², LI Haiying²(1. Beijing Pinggu District Food and Drug Safety Monitoring Center, Beijing 101200,China;2. College of Pharmacy, Hebei University, Baoding 071002,China)Abstract: Pulverized prunella vulgaris was extracted with ethanol or water by semi-bionic method. The contents of ursolic acid(UA)and oleanolic acid(OA)were determined by high performance capillary electrophoresis(HPCE)and compared with those by the traditional ethanol extraction method. HPCE with UV detector at 210 nm was applied using a silica capillary column(50 μm×61 cm, effective length of 50 cm). The sample was injected with a pressure injection of 19.3 MPa for 3 s. The separation buffer was composed of 25 mmol/L sodium tetraborate and 1.5 mmol/L β-cyclodextrin buffer(pH 11). HPCE method was performed at room temperature and the runningvoltage was 14.3 kV. The results showed that the HPCE- 收稿日期:2018-11-21 基金项目:河北省自然科学基金面上项目(H2018201045;H201720105;H2013201203);河北省高等学校科学技术研究项目(ZD2018054;ZD2016102));国家药典委员会、北京市平谷区分区规划编制领导办公室资助项目(2017年第94号). 第一作者:范保瑞(1984-),男,北京市人,北京市平谷区食品药品安全监控中心主管药师,主要从事国家药品质量标准的提高及中药指纹图谱建立. 通信作者:李海鹰(1973-),女,内蒙古集宁人,河北大学副教授,博士,主要从事药物分析及药物制剂方面的研究.E-mail:lihylihy@163.commethod was rapid, simple, convenient and accurate. The contents of UA and OA in the semi-bionic ethanol extract were 4.802mg/g and 0.975mg/g, which were approximately 1.17-fold(4.116 mg/g)and 1.39-fold(0.700 mg/g)of those in traditional ethanol extraction method, respectively. However, the contents of UA and OA in the aqueous extracts were low. Using semi-bionic extraction method, Chinese herbs were extracted using the solution at pH 2.0 for 40 min, pH 7.5 for 40 min and pH 8.3 for 20 min in turn. Which conforms to the law of the human gastrointestinal digestion and transpcrtions. The results shows that The semi-bionic extraction method could be applied to extract two triterpene acids in Prunella vulgaris.

Kinetics, isotherms and mechanisms of Congo red removal by modified Aspergillus fumigatus

JIN Xianchun,SONG Jianing

2019, 39(6):  611-618.  DOI: 10.3969/j.issn.1000-1565.2019.06.008

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The inactivated Aspergillus fumigatus mycelium before and after CTAB modification was used to decolorize Congo red, an anionic azo dye. The results showed that both the A. fumigatus mycelium(AFB)and the modified A. fumigatus mycelium(MAFB)could effectively remove Congo red from dye solution. The kinetics of the overall adsorption process was well described by pseudo-second order kinetic model and the sorption data fit Freundlich isotherm model well, indicating a multilayer adsorption behavior. Chemical modification of the functional groups of A.fumigatus suggested the major involvement of amino groups of A.fumigatus in CR adsorption,while hydroxyl and carboxyl groups played some role. The decolorization mechanism of Congo Red by Aspergillus fumigatus mycelium is described. Below the pH point of zero charge, the amino, hydroxyl and carboxyl groups of the biosorbent are protonated. Therefore,the positive charged biosorbent and the negative charge CR are bound to each other by electrostatic attraction. At the same time, the amino, hydroxyl and carboxyl groups on the biosorbent form hydrogen bonds with Congo Red. While above the pH point of zero charge, the main driving force is the hydrogen- DOI:10.3969/j.issn.1000-1565.2019.06.008改性烟曲霉对刚果红的去除动力学、等温线及机制金显春¹,宋嘉宁²(1.河南农业大学 理学院,河南 郑州 450002;2、河南农业大学 林学院,河南 郑州 450002)摘 要:将十六烷基三甲基溴化铵(CTAB)修饰前后的灭活烟曲霉菌丝体用于对阴离子偶氮染料刚果红的脱色,结果表明改性前烟曲霉菌丝体(AFB)和改性后的烟曲霉菌丝体(MAFB)均可有效去除染料溶液中刚果红.整个吸附过程的动力学过程符合伪二阶动力学模型,吸附数据符合Freundlich等温线模型,表明为多层吸附.采用不同的化学反应封闭烟曲霉的氨基、羧基以及羟基,结果表明烟曲霉氨基是吸附刚果红(CR)的主要基团,而羟基和羧基则起着一定的作用.烟曲霉菌丝对刚果红的脱色机理主要是零电荷点以下pH时,生物吸附剂的氨基、羟基和羧基发生质子化,带正电荷生物吸附剂与带负电荷CR通过静电吸引而被吸附,其次,吸附剂上的氨基、羟基和羧基与刚果红之间形成氢键而进一步被吸附;而零电荷点以上pH时,则主要通过形成氢键进行吸附.关键词:烟曲霉;生物吸附;动力学;机制;等温线中图分类号:S435.72 文献标志码:A 文章编号:1000-1565(2019)06-0611-08Kinetics, isotherms and mechanisms of Congo red removal by modified Aspergillus fumigatusJIN Xianchun¹,SONG Jianing²(1.School of Sciences, Henan Agricultural University, Zhengzhou 450002,China; 2.College of Forestry, Henan Agricultural University, Zhengzhou 450002,China)Abstract:The inactivated Aspergillus fumigatus mycelium before and after CTAB modification was used to decolorize Congo red, an anionic azo dye. The results showed that both the A. fumigatus mycelium(AFB)and the modified A. fumigatus mycelium(MAFB)could effectively remove Congo red from dye solution. The kinetics of the overall adsorption process was well described by pseudo-second order kinetic model and the sorption data fit Freundlich isotherm model well, indicating a multilayer adsorption behavior. Chemical modification of the functional groups of A.fumigatus suggested the major involvement of amino groups of A.fumigatus in CR adsorption,while hydroxyl and carboxyl groups played some role. The decolorization mechanism of Congo Red by Aspergillus fumigatus mycelium is described. Below the pH point of zero charge, the amino, hydroxyl and carboxyl groups of the biosorbent are protonated. Therefore,the positive charged biosorbent and the negative charge CR are bound to each other by electrostatic attraction. At the same time, the amino, hydroxyl and carboxyl groups on the biosorbent form hydrogen bonds with Congo Red. While above the pH point of zero charge, the main driving force is the hydrogen- 收稿日期:2018-09-16 基金项目:河南省高校重点科研项目(15A150051) 第一作者:金显春(1972—),女,湖南郴州人,河南农业大学副教授,博士,主要从事环境污染的微生物治理研究.E-mail:xchjin@163.com第6期金显春:改性烟曲霉对刚果红的去除动力学、等温线及机制bond formed between biosorbents and adsorbate.

Cloning and expression analysis of dct gene in Pelodiscus sinensis

LIU Wenting, XU Peng, ZHANG Na, GUAN Yueqiang, ZHANG Yaohong

2019, 39(6):  619-628.  DOI: 10.3969/j.issn.1000-1565.2019.06.009

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This work aims to clarify the structure and expression characteristics of dct gene in Pelodiscus sinensis, which belongs to tyrosinase family. Primers were designed according mRNA sequence of dct gene in Ensembl online database. Opening reading frame(ORF )region was cloned using RT-PCR and the bioinformatics analysis of dct ORF sequence was carried out. At the same time, mRNAs expression levels of lung, kidney, calipash and muscle tissue in P. sinensis were detected via q-PCR method. The results showed that the full length of ORF of dct gene in P.sinensis was 1 578 bp, encoding a putative 525 aa; The sequence- DOI:10.3969/j.issn.1000-1565.2019.06.009中华鳖dct基因克隆及表达分析刘文婷¹,许鹏²,张娜¹,管越强¹,张耀红³(1. 河北大学 生命科学学院,河北 保定 071002;2. 河北省药品检验研究院,河北 石家庄 050011;3. 保定水产技术推广站,河北 保定 071051)摘 要:为了阐明中华鳖酪氨酸酶家族成员dct基因的结构与表达特征,根据Ensembl数据库的中华鳖dct基因序列设计引物,运用RT-PCR技术进行开放阅读框(opening reading frame,ORF)克隆,并进行生物信息学分析,进而采用实时荧光定量PCR(quantitative real-time PCR,q-PCR)技术检测正常体色和黄色中华鳖肺、肾脏、裙边和肌肉组织的mRNA表达水平.结果显示中华鳖dct基因ORF区全长1 578 bp,编码525个氨基酸,2种体色鳖dct基因序列完全一致;氨基酸序列分析得C末端无L-亮氨酸基序;N末端含有1个信号肽结构域;预测蛋白(成熟肽)分子质量为56.449 ku,理论等电点为6.67,不稳定指数为38.71,表明DCT蛋白是酸性稳定蛋白;预测DCT蛋白为亲水性蛋白;含有跨膜结构域、类表皮生长因子结构域、层粘连蛋白型类表皮生长因子结构域和2个铜离子结合结构域.系统发育树分析显示,在dct基因进化过程中,中华鳖与绿海龟和锦龟的亲缘关系较近.q-PCR结果表明:dct基因在同一个体各组织中均有表达,肺组织中相对表达量最高,且极显著高于其他3种组织;黄色中华鳖肺、肾脏和裙边组织dct 表达水平与正常体色鳖的相应组织均存在显著性差异.结果表明,中华鳖体色变异与dct基因突变无关,但dct基因表达改变可能对体色变异起了一定的作用.关键词:中华鳖;dct基因;克隆;生物信息学;q-PCR中图分类号:S917.4 文献标志码:A 文章编号:1000-1565(2019)06-0619-10Cloning and expression analysis of dct gene in Pelodiscus sinensisLIU Wenting¹, XU Peng², ZHANG Na¹, GUAN Yueqiang¹, ZHANG Yaohong³(1. College of Life Sciences,Hebei University,Baoding 071002, China;2. Hebei Institute for Drug Control and Testing, Shijiazhuang 050011, China;3. Baoding Fishery Technology Extension Station, Baoding 071051, China)Abstract: This work aims to clarify the structure and expression characteristics of dct gene in Pelodiscus sinensis, which belongs to tyrosinase family. Primers were designed according mRNA sequence of dct gene in Ensembl online database. Opening reading frame(ORF )region was cloned using RT-PCR and the bioinformatics analysis of dct ORF sequence was carried out. At the same time, mRNAs expression levels of lung, kidney, calipash and muscle tissue in P. sinensis were detected via q-PCR method. The results showed that the full length of ORF of dct gene in P.sinensis was 1 578 bp, encoding a putative 525 aa; The sequence- 收稿日期:2019-03-19 基金项目:河北省自然科学基金资助项目(C2016201207);河北省现代农业产业技术体系淡水养殖创新团队(HBCT2018180203) 第一作者:刘文婷(1990—),女,安徽合肥人,河北大学在读硕士研究生.E-mail: 280408479@qq.com 通信作者:管越强(1973—),男,河北深泽人,河北大学教授,博士,主要从事水生动物营养与免疫研究.E-mail:guanyueqiang@hbu.edu.cn第6期刘文婷等:中华鳖dct基因克隆及表达分析of dct gene was completely identical in two groups of turtles with different body colours of P. sinensis. The amino acid sequence analysis result showed that L-leocine motif was not observed in the C-terminus. A signal peptide domain was located in the N-terminus. The deduced molecular weight of DCT protein(Mature Peptide)was 56.449 ku, the theoretical isoelectric point was 6.67, and the instability index was 38.71, which indicated it was an acid stable protein. Prediction of hydrophilicity showed that DCT protein was a hydrophilic protein. It contain transmembrane domain, EGF-like domain, laminin-type EGF-like(LE)domain and two copper ion-binding domains. Phylogenetic tree of DCT protein showed that P.sinesis, green sea turtle Chelonia mydas, and Western painted turtle Chrysemys picta clustered into one clade, and the relationship among them was close. The results of q-PCR showed that dct gene was expressed in each detected tissue of the same individual and the expression level of lung tissue was highest and was very significantly higher than the other three detected tissues; In the same tissues of two group with different body colours, there were significant differences in lung, kidney and calipash, respectively.The results showed that the body colour variation of P.sinensis was not related to the mutation of dct gene, and the change of dct gene expression may play a role in the body color variation.

Distribution characteristic of fatty acid in fish tissues from Dongting Lake and its correlation with δ¹⁵N value

ZHANG Kexin, GUO Rui, GE Fangfang, AN Lihui, ZHAO Xingru

2019, 39(6):  629-636.  DOI: 10.3969/j.issn.1000-1565.2019.06.010

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To explore the correlation between fatty acids and trophic level in freshwater fish, six fish species in different trophic levels were collected from Dongting Lake, and were dissected to muscle, liver, kidney, intestine, gill, eye, bladder, skin and lipid tissue. In this study, the stable nitrogen isotope abundance values(δ¹⁵N values)and fatty acid in fish tissues were determined with the stable isotope analysis and gas chromatography-mass spectrometry analysis(GC-MS), respectively. The results indicated that the saturated fatty acids and monounsaturated fatty acids have an even distribution in different fish tissues, while the polyunsaturated fatty acids has obvious differences distribution. It showed an increase with δ¹⁵N value that the ratio of docosahexaenoic acid to eicosapentaenoic acid in the muscle, gill, liver, skin and intestine of fish from Dongting Lake. It suggested that the ratios of DHA/EPA in these tissues could indicate the trophic level of fish from Dongting Lake.- DOI:10.3969/j.issn.1000-1565.2019.06.010洞庭湖鱼中脂肪酸的组织分布特征及其与δ¹⁵N值的相关性张可欣,郭睿,葛芳芳,安立会,赵兴茹(中国环境科学研究院 湖泊水污染治理与生态修复技术国家工程实验室,北京 100012)摘 要:为探究淡水鱼体内脂肪酸与营养级的相关性,采集了洞庭湖6种不同营养级的鱼,将其解剖为肌肉、肝脏、肾脏、肠、鳃、眼、鳔、皮和脂肪等组织样品,采用稳定同位素和气相色谱-质谱联用(GC-MS)分析技术测定不同营养级鱼组织中氮同位素丰度比值(δ¹⁵N值)和脂肪酸的含量. 结果表明,不同鱼组织中,饱和脂肪酸和单不饱和脂肪酸的分布较均匀,而多不饱和脂肪酸的分布却存在明显的差异. 在洞庭湖鱼类的肌肉、肝脏、鳃、皮和肠中二十二碳六烯酸与二十碳五烯酸的比值(DHA/EPA)随δ¹⁵N值的增加而增加,表明鱼组织中的DHA/EPA可以指示洞庭湖淡水鱼类营养级的高低.关键词:洞庭湖;鱼组织;脂肪酸;δ¹⁵N值中图分类号:O657.63 文献标志码:A 文章编号:1000-1565(2019)06-0629-08Distribution characteristic of fatty acid in fish tissues from Dongting Lake and its correlation with δ¹⁵N valueZHANG Kexin, GUO Rui, GE Fangfang, AN Lihui, ZHAO Xingru(National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, Chinese Research Academy of Environmental Sciences, Beijing 100012, China)Abstract: To explore the correlation between fatty acids and trophic level in freshwater fish, six fish species in different trophic levels were collected from Dongting Lake, and were dissected to muscle, liver, kidney, intestine, gill, eye, bladder, skin and lipid tissue. In this study, the stable nitrogen isotope abundance values(δ¹⁵N values)and fatty acid in fish tissues were determined with the stable isotope analysis and gas chromatography-mass spectrometry analysis(GC-MS), respectively. The results indicated that the saturated fatty acids and monounsaturated fatty acids have an even distribution in different fish tissues, while the polyunsaturated fatty acids has obvious differences distribution. It showed an increase with δ¹⁵N value that the ratio of docosahexaenoic acid to eicosapentaenoic acid in the muscle, gill, liver, skin and intestine of fish from Dongting Lake. It suggested that the ratios of DHA/EPA in these tissues could indicate the trophic level of fish from Dongting Lake.- 收稿日期:2019-06-10 基金项目:科技基础性工作专项(2015FY110900-6) 第一作者:张可欣(1994—),女,河北唐山人,河北师范大学硕士研究生,主要从事持久性有机污染物的迁移转化研究. E-mail:1468847709@qq.com 通信作者:赵兴茹(1965—),女,河北定州人,中国环境科学研究院研究员,主要从事持久性有机污染物的迁移转化方向的研究. E-mail: zhaoxr@craes.org.cn第6期张可欣等:洞庭湖鱼中脂肪酸的组织分布特征及其与δ¹⁵N值的相关性

Determination of phenols and antioxidant activity of inflorescence from Scabiosa tschiliensis Grunning

MA Weiwei, HUANG Hangjun, BI Kaili, LI Yi, WANG Junli

2019, 39(6):  637-642.  DOI: 10.3969/j.issn.1000-1565.2019.06.011

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For utilization the resources of Scabiosa tschiliensis Grunning, the content of total phenol in different extracts of inflorescence from S. tschiliensis Grunning was determined. The results showed that the content of total phenol in ethyl acetate fraction was 130.8 mg/g, much high than other fractions. Ten compounds of quercitrin, quercetin, chlorogenic acid, rutin, caffeic acid, L-epicatechin, icariin, ferulic acid, isorhamnetin and vanillic acid were identified from the extracts by HPLC. The contents of quercitrin, quercetin, chlorogenic acid and rutin were high, up to 15.15, 6.87, 4.73 and 2.67 mg/g, respectively. In vitro antioxidant analysis showed that the ethyl acetate fraction had strong antioxidant capacity.

Plant diversity analysis and spatial structure characteristics of Nanyue mountain Forest Park in Jianghan Plain

WEI Pujie,SUN Bing,HU Shengke, ZHU Sijia,Fei Yongjun,HU Die

2019, 39(6):  643-651.  DOI: 10.3969/j.issn.1000-1565.2019.06.012

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Fifteen typical sample plots of Nanyue mountain Forest Park were investigated in the field. The plant diversity and spatial structure characteristics were analyzed by using the diversity indices such as important value, Patrick index, Simpson index, Shannon-Wiener index, Pielou index and the spatial structure parameters such as mingling, neighborhood comparison, angular scale and opening degree. The Nanyue- DOI:10.3969/j.issn.1000-1565.2019.06.012江汉平原南岳山森林公园植物多样性分析与空间结构特征魏普杰¹,孙兵¹,胡胜科¹,朱思甲²,费永俊¹,胡蝶¹(1.长江大学 楠木种质资源评价与创新中心,湖北 荆州 434025;2.桂林理工大学 旅游与风景园林学院,广西 桂林 541004)摘 要:对南岳山森林公园15块典型样地进行了野外实地调查,运用重要值、Patrick指数、Simpson指数、Shannon-Wiener指数、Pielou指数等多样性指数和混交度、大小比数、角尺度、开敞度等林分空间结构参数,分析其植物多样性与空间结构特征.南岳山森林公园植物较丰富,样地共出现32种乔木植物,隶属于28属19科;乔木层中的构树Broussonetia papyrifera、青冈栎Cyclobalanopsis glauca在整个调查区占有明显优势,其重要值大于7.00%.在不同样地的各林层间的Patrick指数和Shannon-Wiener指数均为乔木层>灌木层>草本层,Simpson指数为草本层>灌木层>乔木层,Pielou指数为灌木层>乔木层>草本层;样地林分平均混交度为0.606 2,属中度混交范围,样地林分平均大小比数为0.461 3,属中庸等级,样地林分平均角尺度为0.537 2,属随机分布,聚集程度较低,样地林分的平均开敞度为0.168 7,林木生长空间处于严重不足状态.江汉平原南岳山森林公园的植物多样性较丰富,林木生长空间处于严重不足状态,应该采取补植、间伐相结合的措施,使森林公园生态效益能够可持续发展,通过分析其植物多样性以及空间结构特征,对以后公园的林分空间改造、植物绿化配置以及公园再造设计提供科学依据.关键词:植物多样性;空间结构特征;森林公园;南岳山中图分类号:S718.54 文献标志码:A 文章编号:1000-1565(2019)06-0643-09Plant diversity analysis and spatial structure characteristics of Nanyue mountain Forest Park in Jianghan PlainWEI Pujie¹,SUN Bing¹,HU Shengke¹, ZHU Sijia²,Fei Yongjun¹,HU Die¹(1.Evaluation and Innovation Center of Nanmu Germplasm Resources, Yangtze University, Jingzhou 434025, China;2.College of Tourism and Landscape Architecture, Guilin University of Technology, Guilin 541004,China)Abstract: Fifteen typical sample plots of Nanyue mountain Forest Park were investigated in the field. The plant diversity and spatial structure characteristics were analyzed by using the diversity indices such as important value, Patrick index, Simpson index, Shannon-Wiener index, Pielou index and the spatial structure parameters such as mingling, neighborhood comparison, angular scale and opening degree. The Nanyue- 收稿日期:2019-03-19 基金项目:国家自然科学基金资助项目(31270740);湖北省教育厅科学技术研究项目(Q20181314);湖北省自然科学基金资助项目(2017CFB390);湖北省教育厅指导性项目(B20154444) 第一作者:魏普杰(1994—),男,湖北襄阳人,长江大学在读硕士研究生,主要从事观赏园艺研究. E-mail:1902113155@qq.com 通信作者:费永俊(1965—),男,湖北荆州人,长江大学教授,博士生导师,主要从事园林植物种质资源评价与研究. E-mail:fyj2010@163.com胡蝶(1985—),女,湖北荆州人,长江大学讲师,博士,主要从事系统与进化植物学研究. E-mail:hudie.16@163. com第6期魏普杰等:江汉平原南岳山森林公园植物多样性分析与空间结构特征mountain Forest Park was rich in plants. There were 32 species of arbor plants in the plot, belonging to 28 genera and 19 families. Broussonetia papyrifera and Cyclobalanopsis glauca in the arbor layer had obvious advantages in the whole investigation area, and their important value was greater than 7.00%. The Patrick index and Shannon-Wiener index between different forest layers in different plots was arbor layer>shrub layer>herb layer, Simpson index was herb layer>shrub layer>arbor layer, and Pielou index was shrub layer>arbor Layer>herb layer; the average mingling of the forest stand in plot was 0.606 2, which belonged to the range of moderate mingling. The average neighborhood comparison of the forest stand in plot was 0.461 3, which belonged to the median grade. The average angular scale of the forest stand in plot was 0.537 2, which was a random distribution with low degree of aggregation, the average opening degree of the sample stand in plot was 0.168 7, and the growth space of the forest was seriously insufficient. The plant diversity of Nanyue mountain Forest Park in Jianghan Plain was abundant, and the growth space of trees was seriously insufficient. The measures of combining replanting and thinning should be taken to make the ecological benefits of forest park sustainable. The analysis of plant diversity and spatial structure characteristics provided scientific basis for forest spatial transformation, plant greening configuration and park reconstruction design in the future.

Study on antioxidation of the extract from Polygonum cuspidatum in vivo

WU Jinxia, WANG Shan, LIU Shuang, YUAN Erying, ZHANG Heying

2019, 39(6):  652-656.  DOI: 10.3969/j.issn.1000-1565.2019.06.013

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To study the effect of the extract from Polygonum cuspidatum on antioxidant activity in mice and the anti-aging effect on Drosophila melanogaster in vivo, Three groups of Kunming mice were intragastrically administered with high(0.4 mL), medium(0.2 mL)and low(0.1 mL)of the extract(1g/L concentration)from P. cuspidatum for 4 weeks. The ocular venous blood was taken to determine the total antioxidant capacity of the serum(T-AOC), superoxide dismutase(SOD)activity and malondialdehyde(MDA)content. The D. melanogaster were continuously fed with four different concentrations of the extract from P. cuspidatum(0.1%, 0.2%, 0.4%, 0.8%), and the number of dead D. melanogaster was recorded every 24 hours, and the number of days of D. melanogaster survival was recorded.The results showed that the high dose of the extract from P. cuspidatum significantly increased the T-AOC activity in the serum of mice, and the serum MDA content also decreased significantly, but the serum SOD activity did not increase significantly. 0.2% of the extract from P. cuspidatum significantly prolonged the lifespan of male and female D. melanogaster, the average life expectancy of female D. melanogaster was 31.68%, the average life expectancy of male D. melanogaster was 43.25%, and the maximum life expectancy of female- DOI:10.3969/j.issn.1000-1565.2019.06.013虎杖提取物体内抗氧化作用研究武金霞¹, 王姗¹, 刘爽¹,袁二影¹, 张贺迎²(1.河北大学 生命科学学院,河北 保定 071002;2.河北大学 生物技术研究中心,河北 保定 071002)摘 要:为研究虎杖提取物对小鼠体内抗氧化力的作用以及对果蝇的延缓衰老作用.分别以高(0.4 mL)、中(0.2 mL)、低(0.1 mL)3个剂量虎杖提取物(质量浓度为1 g/L)灌胃昆明种小鼠4周.取眼球静脉血,测定血清的总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量.分别以4个不同浓度虎杖提取物(质量分数0.1%、0.2%、0.4%、0.8%)的基本培养基连续喂养果蝇,每24 h记录一次果蝇死亡数,记录果蝇存活天数.结果表明高剂量虎杖提取物显著提高了小鼠血清中的T-AOC活力,血清MDA含量也显著降低,但血清SOD活力提高不明显.质量分数0.2%虎杖提取物极显著延长雌雄果蝇寿命,对雌果蝇平均寿命延长率为31.68%,对雄果蝇平均寿命延长率为43.25%;对雌果蝇最高寿命延长率为39.05%,对雄果蝇最高寿命延长率为41.62%,添加质量分数0.4%和0.8%虎杖提取物的雌雄果蝇寿命显著延长.虎杖提取物作用于小鼠和果蝇体内,有一定的抗氧化和延缓衰老的作用.关键词:虎杖提取物;小鼠;果蝇;抗氧化;抗衰老中图分类号:Q819 文献标志码:A 文章编号:1000-1565(2019)06-0652-05Study on antioxidation of the extract from Polygonum cuspidatum in vivoWU Jinxia¹, WANG Shan¹, LIU Shuang¹, YUAN Erying¹, ZHANG Heying²(1. College of Life Sciences, Hebei University, Baoding 071002, China;2. Research Center for Biotechnology, Hebei University, Baoding 071002, China)Abstract: To study the effect of the extract from Polygonum cuspidatum on antioxidant activity in mice and the anti-aging effect on Drosophila melanogaster in vivo, Three groups of Kunming mice were intragastrically administered with high(0.4 mL), medium(0.2 mL)and low(0.1 mL)of the extract(1g/L concentration)from P. cuspidatum for 4 weeks. The ocular venous blood was taken to determine the total antioxidant capacity of the serum(T-AOC), superoxide dismutase(SOD)activity and malondialdehyde(MDA)content. The D. melanogaster were continuously fed with four different concentrations of the extract from P. cuspidatum(0.1%, 0.2%, 0.4%, 0.8%), and the number of dead D. melanogaster was recorded every 24 hours, and the number of days of D. melanogaster survival was recorded.The results showed that the high dose of the extract from P. cuspidatum significantly increased the T-AOC activity in the serum of mice, and the serum MDA content also decreased significantly, but the serum SOD activity did not increase significantly. 0.2% of the extract from P. cuspidatum significantly prolonged the lifespan of male and female D. melanogaster, the average life expectancy of female D. melanogaster was 31.68%, the average life expectancy of male D. melanogaster was 43.25%, and the maximum life expectancy of female- 收稿日期:2019-09-04 基金项目:河北省生物工程重点学科资助项目. 第一作者:武金霞(1966—),女,河北保定人,河北大学教授,主要从事微生物学及生物化学研究.E-mail:wjx6@163.com 通信作者:张贺迎(1966—),男,河北保定人,河北大学副研究员,主要从事微生物学研究.E-mail:z660128@163.com第6期武金霞等:虎杖提取物体内抗氧化作用研究D. melanogaster was 39.05%. The maximum life expectancy of male D. melanogaster was 41.62%, and the lifespan of male and female D. melanogaster with 0.4% and 0.8% of the extract from P cuspidatum was significantly prolonged.The extract from P. cuspidatum has certain anti-oxidation. anti-aging effects on mice and D. melanogaster.

Gait feature extraction based on restricted Boltzmann machine and its recognition method

LI Kai, CAO Kefan

2019, 39(6):  657-665.  DOI: 10.3969/j.issn.1000-1565.2019.06.014

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Aiming at gait recognition, the gait feature extraction and recognition of restricted Boltzmann machine(RBM)are studied. Based on the human pedestrian image sequence, the gait energy map is generated by background segmentation, normalization and gait cycle calculation. The gait energy map is used as the feature image of gait, and the gait feature is automatically acquired by RBM. The gait database of Chinese Academy of Sciences CASIA is chosen to study the gait recognition using RBM method for different classification methods, including support vector machine, twin support vector machine, neural network and K-nearest neighbor method. At the same time, this approach is compared with PCA, LDA and CNN feature extraction and recognition.

Moderate resolution remote sensing scene classification method based on multi-scale feature fusion

ZHANG Jun, ZHANG Min, HAO Xiaoke, XIE Peng

2019, 39(6):  666-672.  DOI: 10.3969/j.issn.1000-1565.2019.06.015

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In the scene classification of moderate resolution remote sensing images, the traditional feature extraction method cannot be used to extract comprehensive features, while in the case of scene classification using convolutional neural network, the convolution kernel of the same size cannot extract features in ground objects of different sizes, which reduces the classification accuracy. In order to extract features in different sizes and improve the accuracy of classification, this paper proposes a moderate resolution remote sensing scene classification algorithm based on multi-scale feature fusion. The traditional convolutional neural network was improved to adapt to the moderate resolution remote sensing data set, and multi-scale pooling was added, and multi-level feature maps were input to the full-connection layer for classification. The experiment shows that the feature information extracted by multi-layer feature fusion method is more comprehensive and the classification effect is better than that by single-layer multi-scale pooling method. Compared with other traditional classification methods, our method yields better classification results.- DOI:10.3969/j.issn.1000-1565.2019.06.015基于多尺度特征融合的中分辨率遥感场景分类算法张军^1,2,张敏^1,2,郝小可^1,2,解鹏^1,2(1.河北工业大学 人工智能与数据科学学院,天津 300401;2.河北省大数据计算重点实验室,天津 300401)摘 要:在对中分辨率遥感图像进行场景分类时,传统的特征提取方法无法提取全面的特征,若使用卷积神经网络进行场景分类,同一大小的卷积核无法提取尺寸大小各异的地物特征,导致分类精度降低.为了提取不同尺寸的地物特征,提高分类精度,本文提出一种基于多尺度特征融合的中分辨率遥感场景分类算法.对传统的卷积神经网络进行改进以适应中分辨率遥感数据集,并在其基础上添加多尺度池化,将连接多层次的特征图谱输入到全连接层进行分类.实验表明,多层特征融合方法提取的特征信息比单层多尺度池化方法提取的特征信息更全面,分类效果更优.与其他的传统分类方法相比,本文方法获得更好的分类结果.关键词:卷积神经网络;多层特征融合;多尺度池化;遥感图像场景分类中图分类号:TP399 文献标志码:A 文章编号:1000-1565(2019)06-0666-07Moderate resolution remote sensing scene classification method based on multi-scale feature fusionZHANG Jun^1,2, ZHANG Min^1,2, HAO Xiaoke^1,2, XIE Peng^1,2(1. School of Artificial Intelligence, Hebei University of Technology, Tianjin 300401, China; 2. Hebei Province Key Laboratory of Big Data Calculation, Tianjin 300401, China)Abstract: In the scene classification of moderate resolution remote sensing images, the traditional feature extraction method cannot be used to extract comprehensive features, while in the case of scene classification using convolutional neural network, the convolution kernel of the same size cannot extract features in ground objects of different sizes, which reduces the classification accuracy. In order to extract features in different sizes and improve the accuracy of classification, this paper proposes a moderate resolution remote sensing scene classification algorithm based on multi-scale feature fusion. The traditional convolutional neural network was improved to adapt to the moderate resolution remote sensing data set, and multi-scale pooling was added, and multi-level feature maps were input to the full-connection layer for classification. The experiment shows that the feature information extracted by multi-layer feature fusion method is more comprehensive and the classification effect is better than that by single-layer multi-scale pooling method. Compared with other traditional classification methods, our method yields better classification results.- 收稿日期:2019-02-15 基金项目:国家自然科学青年基金资助项目(61806071);天津市自然科学基金资助项目(16JCYBJC15600);河北省自然科学基金资助项目(F2017202145) 第一作者:张军(1976—),男,河北张家口人,河北工业大学副教授,博士,主要从事智能信息处理研究.E-mail: zhangjun@scse.hebut.edu.cn 通信作者:郝小可(1985—),男,天津市人,河北工业大学讲师,博士,主要从事机器学习、医学图像分析研究.E-mail:haoxiaoke@scse.hebut.edu.cn第6期张军等:基于多尺度特征融合的中分辨率遥感场景分类算法