Effects of different invasion time of Flaveria bidentis on soil microbial function

doi:10.3969/j.issn.1000-1565.2021.02.011

Abstract

Abstract: By using the homologous garden test, Biolog technique was used to compare the utilization of carbon sources in the rhizosphere soil microorganisms of Flaveria bidentis and Setaria viridis and analyzed the changes of functional diversity at different times(5 years, 7 years and 9 years).The results showed that:After different time of invasion,the average well color development(AWCD)values of F+S treatments were higher than those of F treatment under different invasive years.Compared with other treatments,the McIntosh index U of F+S treatment was higher;with the increase of invasion time,the McIntosh index U of F+S treatment increased significantly;the Simpson index D of the F treatment increased significantly,its McIntosh index U gradually decreased and was significantly smaller than the S treatment at 9 years of colonization.Compared with other treatments, F+S treatment promoted the absorption of lipids, sugars and amino acids by soil microorganisms. Compared to colonization for 5 and 7 years,- DOI:10.3969/j.issn.1000-1565.2021.02.011黄顶菊入侵不同时间对土壤微生物功能的影响王妍,杜鄂巍,蒙彦良,孙建茹,桑晓玲,张风娟(河北大学生命科学学院,河北保定 071002)摘要:通过同质园试验,采用Biolog技术比较黄顶菊(Flaveria bidentis)定植不同时间(5年、7年、9年)其与狗尾草根际土壤微生物对碳源的利用能力,分析其功能多样性的变化.结果表明:定植不同时间,黄顶菊与本地植物狗尾草混合种植小区(简称F+S处理)的平均颜色变化率都高于黄顶菊单一种植小区(简称F处理);与其他处理相比,F+S处理的均匀度指数U较高;随着定植时间的延长,F+S处理的均匀度指数U显著增加;F处理的优势度指数D显著增加,其均匀度指数U逐渐下降,并在定植9年时显著小于狗尾草单一种植小区(简称S处理);与其他处理相比,F+S处理促进了土壤微生物对脂类、糖类和氨基酸的吸收,与定植5年和7年相比,定植9年F+S处理的土壤微生物对脂类的利用能力显著提高,对糖类利用能力显著降低.由此可知,黄顶菊入侵后定植不同时间对土壤微生物的功能产生了影响.因此,探究外来植物根际土壤微生物功能随时间变化的一般规律,有助于进一步揭示外来植物的入侵机制.关键词:黄顶菊;土壤微生物;功能多样性;竞争生长;Biolog中图分类号:Q948.11 文献标志码:A 文章编号:1000-1565(2021)02-0180-08Effects of different invasion time of Flaveria bidentis on soil microbial function WANG Yan, DU Ewei, MENG Yanliang, SUN Jianru, SANG Xiaoling, ZHANG Fengjuan(College of Life Sciences, Hebei University, Baoding 071002, China)Abstract: By using the homologous garden test, Biolog technique was used to compare the utilization of carbon sources in the rhizosphere soil microorganisms of Flaveria bidentis and Setaria viridis and analyzed the changes of functional diversity at different times(5 years, 7 years and 9 years).The results showed that:After different time of invasion,the average well color development(AWCD)values of F+S treatments were higher than those of F treatment under different invasive years.Compared with other treatments,the McIntosh index U of F+S treatment was higher;with the increase of invasion time,the McIntosh index U of F+S treatment increased significantly;the Simpson index D of the F treatment increased significantly,its McIntosh index U gradually decreased and was significantly smaller than the S treatment at 9 years of colonization.Compared with other treatments, F+S treatment promoted the absorption of lipids, sugars and amino acids by soil microorganisms. Compared to colonization for 5 and 7 years,- 收稿日期:2020-04-16 基金项目:国家自然科学基金资助项目(31972343);河北省自然科学基金资助项目(C2019201059);河北大学研究生创新项目(hbu2019ss026) 第一作者:王妍(1995—),女,山东济南人,河北大学硕士研究生,主要从事入侵生物学研究.E-mail:1007276126@qq. com 通信作者:张风娟(1969—),女,河北唐山人,河北大学教授,博士,主要从事植物学与生态学方向研究.E-mail:Fengjuanzhang@126.com第2期王妍等:黄顶菊入侵不同时间对土壤微生物功能的影响soil microorganisms in F+S treatment significantly improved the utilization of lipids and reduced the utilization of sugars in F. bidentis colonization for 9 years, respectively. It can be seen that the different times of F. bidentis colonization have an impact on the function of soil microorganism.Therefore, exploring the general laws of the function of alien plant rhizosphere soil microorganisms over time will help to further reveal the invasion mechanism of alien plants.

Key words: Flaveria bidentis, soil microbes, functional diversity, competitive growth, Biolog

CLC Number:

Q948.11

WANG Yan, DU Ewei, MENG Yanliang, SUN Jianru, SANG Xiaoling, ZHANG Fengjuan. Effects of different invasion time of Flaveria bidentis on soil microbial function[J]. Journal of Hebei University(Natural Science Edition), 2021, 41(2): 180-187.

References

[1] GELDNER N, SALT D E. Focus on roots[J]. Plant Physiology, 2014, 166(2): 453-454. DOI:10.1104/pp.114.900494.
[2] MEHTA P, WALIA A, KULSHRESTHA S, et al. Efficiency of plant growth-promoting P-solubilizing Bacillus circulansCB₇ for enhancement of tomato growth under net house conditions[J]. J Basic Microbiol, 2015, 55(1): 33-44. DOI:10.1002/jobm.201300562.
[3] KAMUTANDO C N, VIKRAM S, KAMGAN-NKUEKAM G, et al. The functional potential of the rhizospheric microbiome of an invasive tree species, acacia dealbata[J]. Microb Ecol, 2019, 77(1): 191-200. DOI:10.1007/s00248-018-1214-0.
[4] VIVANCO J M, BAIS H P, STERMITZ F R, et al. Biogeographical variation in community response to root allelochemistry: novel weapons and exotic invasion[J]. Ecol Lett, 2004, 7(4): 285-292. DOI:10.1111/j.1461-0248.2004.00576.x.
[5] SHANNON S, FLORY S L, REYNOLDS H. Competitive context alters plant-soil feedback in an experimental woodland community[J]. Oecologia, 2012, 169(1): 235-243. DOI:10.1007/s00442-011-2195-6.
[6] ZUBEK S, MAJEWSKA M L, BLASZKOWSKI J, et al. Invasive plants affect arbuscular mycorrhizal fungi abundance and species richness as well as the performance of native plants grown in invaded soils[J]. Biol Fertil Soils, 2016, 52(6): 879-893. DOI:10.1007/s00374-016-1127-3.
[7] RODGERS V L, WOLFE B E, WERDEN L K, et al. The invasive species Alliaria petiolata(garlic mustard)increases soil nutrient availability in northern hardwood-conifer forests[J]. Oecologia, 2008, 157(3): 459-471. DOI:10.1007/s00442-008-1089-8.
[8] 杨星.黄顶菊对土壤中微生物群落、酶活性及养分的影响[D].保定:河北农业大学,2012.
[9] CRAWFORD K M, KNIGHT T M. Competition overwhelms the positive plant-soil feedback generated by an invasive plant[J]. Oecologia, 2017, 183(1): 211-220. DOI:10.1007/s00442-016-3759-2.
[10] 于文清,万方浩,何新华,等.土壤微生物增强了外来植物紫茎泽兰对本地植物种的竞争力[J].生物安全学报,2014,23(3): 156-164. DOI:10.3969/j.issn.2095-1787.2014.03.004.
[11] DUDA J J, FREEMAN D C, EMLEN J M, et al. Differences in native soil ecology associated with invasion of the exotic annual chenopod, Halogetonglomeratus[J]. Biol Fertil Soils, 2003, 38(2): 72-77. DOI:10.1007/s00374-003-0638-x.
[12] 陈学林,张丽娜,王桔红,等.红毛草入侵程度对根际土壤微生物的影响[J].西北农林科技大学学报(自然科学版),2017,45(4): 165-172. DOI:10.13207/j.cnki.jnwafu.2017.04.023.
[13] 郑志鑫,王瑞,张风娟,等.外来入侵植物黄顶菊在我国的地理分布格局及其时空动态[J].生物安全学报,2018,27(4): 295-299. DOI:10.3969/j.issn.2095-1787.2018.04.013.
[14] MA J W, GENG S L, WANG S B, et al. Genetic diversity of the newly invasive weed Flaveria bidentis(Asteraceae)reveals consequences of its rapid range expansion in Northern China[J]. Weed Res, 2011, 51(4): 363-372. DOI:10.1111/j.1365-3180.2011.00856.x.
[15] SONG Z, ZHANG R H, FU W D, et al. High-throughput sequencing reveals bacterial community composition in the rhizosphere of the invasive plant Flaveria bidentis[J]. Weed Res, 2017, 57(3): 204-211. DOI:10.1111/wre.12250.
[16] 纪巧凤.黄顶菊入侵对根际土壤主要功能细菌多样性的影响[D].北京:中国农业科学院,2014.
[17] CHEN X W, LIU Y X, LIU H M, et al. Impacts of four invasive Asteraceae on soil physico-chemical properties and AM fungi community[J]. Am J Plant Sci, 2015, 6(17): 2734-2743. DOI:10.4236/ajps.2015.617274.
[18] 赵晓红,杨殿林,曲波,等.黄顶菊入侵对土壤氨氧化细菌群落结构及多样性的影响[J].生态学杂志,2014,33(6): 1492-1500. DOI:10.13292/j.1000-4890.20140327.027.
[19] 赵天心,毛新伟,程敏,等.毛竹种植对土壤细菌和真菌群落结构及多样性的影响[J].应用生态学报,2017,28(11): 3740-3750. DOI:10.13287/j.1001-9332.201711.033.
[20] 党雯,郜春花,张强,等.Biolog法测定土壤微生物群落功能多样性预处理方法的筛选[J].中国农学通报,2015,31(2): 153-158.
[21] 赵紫檀,郑洁,吴则焰,等.互花米草入侵下红树林土壤微生物群落特征[J].森林与环境学报,2017,37(2): 169-173. DOI:10.13324/j.cnki.jfcf.2017.02.007.
[22] 侯晓杰,汪景宽,李世朋.不同施肥处理与地膜覆盖对土壤微生物群落功能多样性的影响[J].生态学报,2007,27(2): 655-661. DOI:10.3321/j.issn: 1000-0933.2007.02.029.
[23] SH GALIEVA G, GILMUTDINOVA I M, FOMIN V P, et al. Monitoring soil bacteria with community-level physiological profiles using biolog^TM ECO-plates in the republic of Tatarstan(Russia)[J]. IOP ConfSer: Earth Environ Sci, 2018, 107: 012057. DOI:10.1088/1755-1315/107/1/012057.
[24] ODONNELL A G, SEASMAN M, MACRAE A, et al. Plants and fertilisers as drivers of change in microbial community structure and function in soils[M] //Interactions in the Root Environment: An Integrated Approach. Dordrecht: Springer Netherlands, 2002: 135-145. DOI:10.1007/978-94-010-0566-1_14.
[25] 陈华.外来植物与土壤微生物的关系研究[D].济南:山东大学,2011.
[26] SIMPSON E H. Measurement of diversity[J]. Nature, 1949, 163(4148): 688. DOI:10.1038/163688a0.
[27] HUANGFU C H, LI H Y, CHEN X W, et al. The effects of exotic weed Flaveria bidentis with different invasion stages on soil bacterial community structures[J]. Afr J Biotechnol, 2015, 14(35): 2636-2643. DOI:10.5897/ajb2015.14658.
[28] DE DEYN G B, RAAIJMAKERS C E, VAN DER PUTTEN W H. Plant community development is affected by nutrients and soil biota[J]. J Ecol, 2004, 92(5): 824-834. DOI:10.1111/j.0022-0477.2004.00924.
[29] 牛红榜,刘万学,万方浩.紫茎泽兰(Ageratina adenophora)入侵对土壤微生物群落和理化性质的影响[J].生态学报, 2007, 27(7): 3051-3060. DOI:10.3321/j.issn: 1000-0933.2007.07.047.
[30] 区余端,苏志尧,彭桂香,等.车八岭山地常绿阔叶林冰灾后土壤微生物群落功能多样性[J].生态学报,2009,29(11): 6156-6164. DOI:10.3321/j.issn: 1000-0933.2009.11.050.
[31] LI H Y, WEI Z S, HUANGFU C H, et al. Litter mixture dominated by leaf litter of the invasive species, Flaveria bidentis, accelerates decomposition and favors nitrogen release[J]. J Plant Res, 2017, 130(1): 167-180. DOI:10.1007/s10265-016-0881-5.
[32] 宋振,纪巧凤,付卫东,等.黄顶菊入侵对土壤中主要功能细菌的影响[J].应用生态学报,2016, 27(8): 2636-2644. DOI:10.13287/j.1001-9332.201608.013.
[33] 赵晓红,皇甫超河,曲波,等.黄顶菊(Flaveria bidentis)入侵对土壤微生物功能多样性的影响[J].农业资源与环境学报,2014,31(2): 182-189. DOI:10.13254/j.jare.2014.0044.
[34] KOURTEV P S, EHRENFELD J G, HÄGGBLOM M. Exotic plant species alter the microbial community structure and function in the soil[J]. Ecology, 2002, 83(11): 3152-3166. DOI:10.1890/0012-9658(2002)083[3152: EPSATM] 2.0.CO;2.
[35] ZHANG S S, ZHU W J, WANG B, et al. Secondary metabolites from the invasive Solidago canadensis L. accumulation in soil and contribution to inhibition of soil pathogen Pythium ultimum[J]. Appl Soil Ecol, 2011, 48(3): 280-286. DOI:10.1016/j.apsoil.2011.04.011.
[36] LI Y P, FENG Y L, CHEN Y J, et al. Soil microbes alleviate allelopathy of invasive plants[J]. Sci Bull, 2015, 60(12): 1083-1091. DOI:10.1007/s11434-015-0819-7. (