Simulation and experimental analysis of calcium signaling and synaptic transmission characteristics of cortex under transcranial magneto-acoustic-electrical stimulation

doi:10.3969/j.issn.1000-1565.2023.03.012

Abstract

Abstract: In order to explore the effects of different magnetic field intensities and ultrasonic power intensities of transcranial magneto-acoustic-electrical stimulation(TMAES)on the calcium signaling and synaptic transmission characteristics of cortical neurons related to the encoding of working memory information, this paper builds a cortical pyramidal neuron model based on magneto acoustic effect,- DOI:10.3969/j.issn.1000-1565.2023.03.012经颅磁声电刺激下皮层钙信号及突触传递特性的仿真与实验分析赵清扬, 张帅, 杜文静, 徐亦豪(河北工业大学河北省生物电磁与神经工程重点实验室(筹),天津市生物电工与智能健康重点实验室, 天津 300130)摘要:为了探究经颅磁声电刺激(transcranial magneto-acoustic-electrical stimulation,TMAES)不同磁场强度和超声功率强度对工作记忆信息编码相关的皮层神经元钙信号及突触传递特性的影响,首先通过搭建基于磁声电效应改进的皮层锥体神经元模型,引入钙依赖神经递质释放的计算方法以计算TMAES引起的兴奋性突触后电位(excitatory postsynaptic potential,EPSP),以EPSP作为评价指标来评估不同TMAES磁场强度和超声功率强度下突触传递的短时程可塑性.随后使用光纤光度检测技术实时记录TMAES下小鼠前额叶皮层神经集群的钙信号,以揭示TMAES下钙依赖神经信息传递机制.仿真结果表明:TMAES不同磁场强度和超声功率强度对突触后响应的大小具有双向调节作用,其中,突触传递产生的短时程增强和抑制是由于TMAES下胞内钙浓度的变化引起的囊泡释放和囊泡耗竭.实验结论表明:TMAES对前额叶皮层神经元集群钙信号幅度和频率均有明显的调节作用,TMAES可以通过调节神经钙浓度进而影响突触间的信息传递.关键词:经颅磁声电刺激;钙离子浓度;突触传递;短时程可塑性;兴奋性突触后电位中图分类号:R318.04 文献标志码:A 文章编号:1000-1565(2023)03-0310-10Simulation and experimental analysis of calcium signaling and synaptic transmission characteristics of cortex under transcranial magneto-acoustic-electrical stimulationZHAO Qingyang, ZHANG Shuai, DU Wenjing, XU Yihao(Tianjin key laboratory of Bioelectromagnetism Technology and Intelligent Health,Hebei key laboratory of Bioelectromagnetism and Neural Engineering, Hebei University of Technology, Tianjin 300130, China)Abstract: In order to explore the effects of different magnetic field intensities and ultrasonic power intensities of transcranial magneto-acoustic-electrical stimulation(TMAES)on the calcium signaling and synaptic transmission characteristics of cortical neurons related to the encoding of working memory information, this paper builds a cortical pyramidal neuron model based on magneto acoustic effect,- 收稿日期:2022-12-23 基金项目:国家自然科学基金资助项目(51877069);河北省自然科学基金资助项目(E2021202184) 第一作者:赵清扬(1996—),男,河北张家口人,河北工业大学在读硕士研究生,主要从事生物电磁与神经工程方向研究. E-mail:qyang2023@163.com 通信作者:张帅(1978—),男,河北保定人,河北工业大学教授,主要从事生物电磁与神经工程方向研究. E-mail:zs@hebut.edu.cn第3期赵清扬等:经颅磁声电刺激下皮层钙信号及突触传递特性的仿真与实验分析introduces a calculation method for calcium-dependent neurotransmitter release to calculate the excitatory postsynaptic potential(EPSP)caused by TMAES, and uses EPSP as an evaluation index to evaluate the short-term plasticity of synaptic transmission under different TMAES magnetic field strength and ultrasonic power intensity. Subsequently, the calcium signal of mouse prefrontal cortex nerve clusters under TMAES was recorded in real time using fiber photometric detection technology to reveal the calcium-dependent neural information transmission mechanism under TMAES. The simulation results show that different magnetic field strength and ultrasonic power intensity of TMAES have bidirectional adjustment effects on the size of the postsynaptic response. Among them, short-term enhancement and inhibition of synaptic transmission is due to vesicle release and vesicle depletion caused by changes in intracellular calcium concentration under TMAES. The experimental results show that TMAES has a significant regulatory effect on the amplitude and frequency of calcium signaling in the prefrontal cortex neuronal cluster, and TMAES can affect the information transmission between synapses by regulating the calcium concentration of nerves.

Key words: transcranial magneto-acoustic-electrical stimulation(TMAES), calcium concentration, synaptic transmission, short-term plasticity, excitatory postsynaptic potential

CLC Number:

R318.04

ZHAO Qingyang, ZHANG Shuai, DU Wenjing, XU Yihao. Simulation and experimental analysis of calcium signaling and synaptic transmission characteristics of cortex under transcranial magneto-acoustic-electrical stimulation[J]. Journal of Hebei University(Natural Science Edition), 2023, 43(3): 310-319.

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