Effect of nitrite on start-up of anammox EGSB reactor

doi:10.3969/j.issn.1000-1565.2023.06.005

Abstract

Abstract: Long-term stored anaerobic ammonia oxidation sludge at 4 ℃ was inoculated to investigate the effect of influent nitrite on Anammox expanded granular sludge bed(EGSB)reactor start-up at 50.0 mg/L(R1)and 70.0 mg/L(R2). It was found that the retardation time of R1 was 18 days shorter than that of R2, and higher concentrations of nitrite(70.0 mg/L)inhibited the start-up of the Anammox EGSB reactor. R1 and R2 were successfully started at 53 d and 56 d, with total nitrogen(TN)removal rates of 67.9% and 68.8%, respectively. After increasing the nitrogen load, the ammonia removal rates of R1 and R2 increased from 93.1% and 88.0%, respectively, to 99.6% and 99.0%. The hydraulic shock experiment discovered that external circulation could enhance the substrate mixing effect in the reactor and improve the effluent water quality, and R1 and R2 recovered on the 5th and 6th days following destabilization, respectively. High-throughput sequencing revealed that the dominant genus in both reactors was Candidatus_Jettenia with a relative abundance of 54.1% and 45.3%, respectively. Long-term stored Anammox sludge was initiated with a nitrite concentration recommended below 50.0 mg/L.

Key words: anammox, nitrite, EGSB

CLC Number:

SHI Yonghui, ZHANG Yihao, QIN Zhe, SHI Tianxi, WU Xinying, ZHAO Chunxia, LU Da. Effect of nitrite on start-up of anammox EGSB reactor[J]. Journal of Hebei University(Natural Science Edition), 2023, 43(6): 595-602.

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