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25 November 2023, Volume 43 Issue 6

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Vibration characteristics analysis of the inner race fault of axlebox bearing under wheel-rail excitation

DENG Feiyue,WANG Hongli,GAO Ruiyang,LI Hao

2023, 43(6):  561-570.  DOI: 10.3969/j.issn.1000-1565.2023.06.001

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Wheel-rail complex excitation has a significant effect on the dynamic behavior of faulty axlebox bearing during the train operation. Firstly, this paper uses the Universal Mechanism(UM)software to establish a vehicle-track dynamic model, and obtains the vertical, longitudinal and lateral loads of axlebox bearing under wheel-rail complex excitation based on the verification of vehicle models for stability, smoothness and safety. Secondly, the dynamic model of axlebox bearing with inner race spalling is established via Solidworks and ADAMAS softwares, and the accuracy of the proposed model is verified by comparison with the axlebox bearing experiments on the roll-vibration test bench. The dynamic simulation analysis shows that the contact load of the roller and inner race of the fault side is greater than that of the non-faulty side and the normal bearing, the cage vibration of the fault side is greater than that of- DOI:10.3969/j.issn.1000-1565.2023.06.001轮轨激励条件下轴箱轴承内圈故障振动特性分析邓飞跃^1,2,王红力¹,高瑞洋¹,李浩¹(1.石家庄铁道大学 机械工程学院,河北 石家庄 050043;2.河北省工程机械动力与传动控制重点实验室,河北 石家庄 050043)摘 要:高速列车运行过程中,轮轨复杂激励会对轴箱轴承的动力学行为产生不可忽视的影响.首先利用UM(universal mechanism)软件建立车辆-轨道动力学模型,在对车辆模型进行稳定性、平稳性和安全性验证的基础上,获取了复杂激励下轴箱轴承所受的垂向、纵向和横向载荷;然后,通过Solidworks软件和ADAMS软件建立了轴箱轴承内圈剥离故障动力学模型,通过与滚振实验台轴箱轴承实验对比,验证了所提模型的准确性.通过动力学仿真分析可知,轴箱轴承故障侧的滚子与内圈接触载荷大于非故障侧与正常轴承,故障侧保持架的振动大于非故障侧与正常轴承,内圈故障冲击加剧了轴承保持架与外圈的质心波动.最后,进一步对比考虑轮轨激励下与定载荷下故障轴承仿真结果发现,受轮轨激励的影响轴承内部各个元件间的接触载荷显著增大,轴承保持架与外圈质心运动轨迹盒维数显著增大.研究成果对揭示实际工况下高速动车组轴箱轴承内部元件振动特性规律具有重要意义.关键词:轴箱轴承;轮轨激励;振动特性;内圈故障中图分类号:TH17 文献标志码:A 文章编号:1000-1565(2023)06-0561-10Vibration characteristics analysis of the inner race fault of axlebox bearing under wheel-rail excitationDENG Feiyue^1,2,WANG Hongli¹,GAO Ruiyang¹,LI Hao¹(1.School of Mechanical Engineering, Shijiazhuang Tiedao University, Shijiazhuang 050043,China;2.Hebei Key Laboratory of Mechanical Power and Transmission Control,Shijiazhuang 050043,China)Abstract: Wheel-rail complex excitation has a significant effect on the dynamic behavior of faulty axlebox bearing during the train operation. Firstly, this paper uses the Universal Mechanism(UM)software to establish a vehicle-track dynamic model, and obtains the vertical, longitudinal and lateral loads of axlebox bearing under wheel-rail complex excitation based on the verification of vehicle models for stability, smoothness and safety. Secondly, the dynamic model of axlebox bearing with inner race spalling is established via Solidworks and ADAMAS softwares, and the accuracy of the proposed model is verified by comparison with the axlebox bearing experiments on the roll-vibration test bench. The dynamic simulation analysis shows that the contact load of the roller and inner race of the fault side is greater than that of the non-faulty side and the normal bearing, the cage vibration of the fault side is greater than that of- 收稿日期:2023-06-13 基金项目:国家自然科学基金资助项目(12272243); 河北省研究生专业学位精品教学案例库项目(KCJPZ2023037) 第一作者:邓飞跃(1985—),男,河北石家庄人,石家庄铁道大学副教授,博士,博士生导师,主要从事机电系统故障诊断与状态监测研究.E-mail:dengfy@stdu.edu.cn第6期邓飞跃等:轮轨激励条件下轴箱轴承内圈故障振动特性分析the non-faulty side and the normal bearing, and the fault impact aggravates the trajectory of the mass center fluctuation of the bearing cage and outer ring. Finally, further comparison of the simulation results under the consideration of wheel-rail excitation and the constant load condition reveals that the contact load between the bearing components is significantly increased by the wheel-rail excitation,and the trajectories of the mass center for the bearing cage and outer ring fluctuate more drastically. The research results are of great significance in revealing the vibration characteristics law of the bearing internal components of faulty axlebox bearing under actual operating conditions.

Fault detection method of bearings based on HHO-CNN

LIU Yuxin, WU Wenbo, ZHANG Xiong, WAN Shuting

2023, 43(6):  571-583.  DOI: 10.3969/j.issn.1000-1565.2023.06.002

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Bearing is an important supporting component of drive system, and it is also the weak link of the whole system. The powerful feature extraction and learning ability of convolutional neural network(CNN)provides the possibility for the pattern recognition of the idler bearing. Aiming at the problems of low accuracy and slow rate of convergence caused by the super parameter problem in using CNN to process multi classification pattern recognition, a CNN classification model optimized based on HHO algorithm was proposed. Firstly, the bearing fault dataset is partitioned with different fault types and degrees, and initializes the CNN model parameters; Then, the HHO algorithm is used to optimize the CNN model, calculate fitness values, and obtain the number of units and iteration times of the fully connected layer; Finally,- DOI:10.3969/j.issn.1000-1565.2023.06.002基于HHO-CNN的轴承故障诊断方法研究刘玉鑫¹,武文博²,张雄²,万书亭²(1.国家电投内蒙古能源有限公司 通辽霍林河坑口发电有限责任公司,内蒙古 通辽 028000;2.华北电力大学 机械工程系,河北 保定 071003)摘 要:轴承是传动系统重要的支撑部件,也是整个系统的薄弱环节,卷积神经网络(convolutional neural network,CNN)强大的特征提取和学习能力为轴承运行状态模式识别提供了可能性.针对CNN处理多分类模式识别过程中,由超参数问题引起的准确率低、收敛速度慢等问题,提出了一种基于哈里斯鹰优化(Harris hawks optimization,HHO)算法优化的CNN分类模型.首先,对不同故障类型和故障程度轴承故障数据集进行划分,初始化CNN模型参数;然后,使用HHO算法对CNN模型的超参数空间进行优化,计算适应度值并获取全连接层的单元数量和迭代次数;最后,利用优化后的CNN模型对轴承数据集进行模式识别.通过不同故障类型和故障程度轴承实验数据验证,表明HHO-CNN模型可以使得全连接层的单元数量和迭代次数迅速收敛,及时准确调整CNN的网络参数,提升分类器的性能,提高了故障模式识别准确性,增强了模型的稳定性.关键词:HHO;CNN;轴承;故障诊断中图分类号:TH133.3;TP181 文献标志码:A 文章编号:1000-1565(2023)06-0571-13Fault detection method of bearings based on HHO-CNNLIU Yuxin¹, WU Wenbo², ZHANG Xiong², WAN Shuting²(1.Tongliao Huolinhe Kengkou Power Generation Co. Ltd., State Power Investment Inner Mongolia Energy Co. Ltd.,Tongliao 028000, China;2.Department of Mechanical Engineering, North China Electric Power University, Baoding 071003, China)Abstract: Bearing is an important supporting component of drive system, and it is also the weak link of the whole system. The powerful feature extraction and learning ability of convolutional neural network(CNN)provides the possibility for the pattern recognition of the idler bearing. Aiming at the problems of low accuracy and slow rate of convergence caused by the super parameter problem in using CNN to process multi classification pattern recognition, a CNN classification model optimized based on HHO algorithm was proposed. Firstly, the bearing fault dataset is partitioned with different fault types and degrees, and initializes the CNN model parameters; Then, the HHO algorithm is used to optimize the CNN model, calculate fitness values, and obtain the number of units and iteration times of the fully connected layer; Finally,- 收稿日期:2023-06-13 基金项目:国家自然科学基金资助项目(52105098);河北省自然科学基金资助项目(E2021502038) 第一作者:刘玉鑫(1979—),男,内蒙古通辽人,国家电投内蒙古能源有限公司高级工程师,主要从事电力系统科技创新与管理研究.E-mail:zxlvbzy@163.com 通信作者:张雄(1990—),男,河北保定人,华北电力大学副教授,博士,主要从事旋转机械设备状态检测与故障诊断研究. E-mail:hdjxzx@ncepu.edu.cn第6期刘玉鑫等:基于HHO-CNN的轴承故障诊断方法研究the optimized CNN model is used for pattern recognition on the bearing dataset. Through experimental data of bearings, it is shown that the HHO-CNN model can quickly converge the number of units and iteration times in the fully connected layer, adjust the network parameters of the CNN in a timely and accurate manner, improve the accuracy of fault pattern recognition, and enhance the stability of the model.

Design of cooling system of air warm plasma jet generator

LIU Zhenhua, HUANG Baowang, LI Zhanxian

2023, 43(6):  584-588.  DOI: 10.3969/j.issn.1000-1565.2023.06.003

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A gas loop warm plasma jet generator with air as the working gas as well as cooling media was designed by optimizing the generator electrode and cooling structure in order to better meet the requirements of portable, simple operation, and diverse application backgrounds of plasma jet generator. The impacts of gas flow rate, discharge frequency, and discharge voltage on plasma jet temperature were studied. It was found that the introduction of cooling gas obviously reduces the jet temperature. Under the conditions of 5.5 kV voltage, 22 kHz frequency and 7 L/min flow rate, the introduction of cooling gas reduced the jet temperature by 302 ℃. The jet temperature decreased with the increase of gas flow rate and discharge frequency, but increased with the increase of discharge voltage. The decrease of jet temperature was more dependent on gas flow rate than discharge frequency or voltage.

Evaluation methods of luminous-flux-attenuation activation energy for LED bulb lamp

WU Deqi,WU Yiping,CHU Xiaoxing

2023, 43(6):  589-594.  DOI: 10.3969/j.issn.1000-1565.2023.06.004LED

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Under the rated power, temperature stress accelerated life test of BPZ 220/5 W JA55 bulb lamp was performed under thermal stress of 40 ℃ and 60 ℃ respectively, and the failure mechanism of the LED bulb samples was analyzed. A method to evaluate luminous-flux-attenuation activation energy value for LED bulb lamps was proposed, and a theoretical model to calculate luminous-flux-attenuation activation energy value was established in this paper. Thereby, the luminous-flux attenuation activation energy value of 0.67 eV was determined based on the above mentioned theory.

Effect of nitrite on start-up of anammox EGSB reactor

SHI Yonghui, ZHANG Yihao, QIN Zhe, SHI Tianxi, WU Xinying, ZHAO Chunxia, LU Da

2023, 43(6):  595-602.  DOI: 10.3969/j.issn.1000-1565.2023.06.005

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Long-term stored anaerobic ammonia oxidation sludge at 4 ℃ was inoculated to investigate the effect of influent nitrite on Anammox expanded granular sludge bed(EGSB)reactor start-up at 50.0 mg/L(R1)and 70.0 mg/L(R2). It was found that the retardation time of R1 was 18 days shorter than that of R2, and higher concentrations of nitrite(70.0 mg/L)inhibited the start-up of the Anammox EGSB reactor. R1 and R2 were successfully started at 53 d and 56 d, with total nitrogen(TN)removal rates of 67.9% and 68.8%, respectively. After increasing the nitrogen load, the ammonia removal rates of R1 and R2 increased from 93.1% and 88.0%, respectively, to 99.6% and 99.0%. The hydraulic shock experiment discovered that external circulation could enhance the substrate mixing effect in the reactor and improve the effluent water quality, and R1 and R2 recovered on the 5th and 6th days following destabilization, respectively. High-throughput sequencing revealed that the dominant genus in both reactors was Candidatus_Jettenia with a relative abundance of 54.1% and 45.3%, respectively. Long-term stored Anammox sludge was initiated with a nitrite concentration recommended below 50.0 mg/L.

Role of Mahonia bealei in regulating Ferroptosis by AURKA to intervene in Liver hepatocellular carcinoma

LI Jun, YANG Kaiping, LIN Dengmei, ZHU Yi, ZHANG Nannan

2023, 43(6):  603-615.  DOI: 10.3969/j.issn.1000-1565.2023.06.006

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Through network pharmacology and bioinformatics, we explored the mechanism of Mahonia bealei regulating ferroptosis to interfere with liver hepatocellular carcinoma(LIHC). LIHC gene chip data set was downloaded from GEO database, ferroptosis markers were downloaded from FerrDb database, components and targets of M.bealei were obtained from TCMSP, SymMap and SwissTargetPrediction database. Molecular docking was performed using DS BIOVIA Discovery Studio 2016 v16.1 software to search for hub genes; Using Cytoscape software and STRING platform, PPI analysis was performed on the intersection targets of drug-disease-ferroptosis. The GEPIA and TIMER database were used for differential- DOI:10.3969/j.issn.1000-1565.2023.06.006阔叶十大功劳作用于AURKA调控铁死亡干预肝癌的作用李军¹,杨凯平²,林登梅²,朱燚²,张楠楠¹(1.贵州中医药大学 基础医学院,贵州 贵阳 550025;2.贵州中医药大学 药学院,贵州 贵阳 550025)摘 要:探索阔叶十大功劳Mahonia bealei调控铁死亡干预肝癌的机制.从GEO数据库下载肝癌的转录组数据集,从FerrDb数据库下载铁死亡相关基因,使用TCMSP、SymMap、SwissTargetPrediction数据库获取M.bealei的成分与靶点;使用DS BIOVIA Discovery Studio 2016 v16.1软件进行分子对接;运用Cytoscape软件及STRING平台,对药物-疾病-铁死亡交集靶点进行PPI分析;利用GEPIA、TIMER数据库进行差异表达分析;利用UALCAN数据库进行临床相关性分析;利用Kaplan-Meier Plotter数据库进行生存分析;使用THPA数据库进行病理学切片分析;使用Western blot进行体外实验验证;最后,进行转录因子的预测,共筛选出5个M.bealei调控铁死亡干预肝癌的关键基因,通过基因差异表达选取3个核心基因AKR1C3、AKR1C1、AURKA,进一步分析发现AURKA与肝癌生存分析、临床参数、病理学结果均显著相关.Western blot实验证实,与HepG2肝癌细胞组相比,M.bealei含药血清组的AURKA蛋白表达上调(P<0.05).通过以上研究认为M.bealei可通过作用于AURKA调控铁死亡而干预肝癌.关键词:阔叶十大功劳;肝癌;铁死亡;AURKA 中图分类号:R273 文献标志码:A 文章编号:1000-1565(2023)06-0603-13Role of Mahonia bealei in regulating Ferroptosis by AURKA to intervene in Liver hepatocellular carcinoma LI Jun¹, YANG Kaiping², LIN Dengmei², ZHU Yi², ZHANG Nannan¹(1. School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China; 2. School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China)Abstract: Through network pharmacology and bioinformatics, we explored the mechanism of Mahonia bealei regulating ferroptosis to interfere with liver hepatocellular carcinoma(LIHC). LIHC gene chip data set was downloaded from GEO database, ferroptosis markers were downloaded from FerrDb database, components and targets of M.bealei were obtained from TCMSP, SymMap and SwissTargetPrediction database. Molecular docking was performed using DS BIOVIA Discovery Studio 2016 v16.1 software to search for hub genes; Using Cytoscape software and STRING platform, PPI analysis was performed on the intersection targets of drug-disease-ferroptosis. The GEPIA and TIMER database were used for differential- 收稿日期:2023-04-19 基金项目:贵州中医药大学学术新苗项目(贵科合学术新苗2023-28);贵州省科技计划项目(黔科合基础ZK［2023］一般428);2023年贵州中医药大学研究生教育创新计划项目(YCXKYB2023001) 第一作者:李军(1986—),男,山东菏泽人,贵州中医药大学副教授,主要从事肝癌及糖尿病药物方面研究.E-mail: lijun763@gzy.edu.cn 通信作者:张楠楠(1989—),女,河南焦作人,贵州中医药大学讲师,博士,主要从事肝癌及糖尿病药物研究.E-mail: doczn@sina.com第6期李军等:阔叶十大功劳作用于AURKA调控铁死亡干预肝癌的作用expression analysis. Clinical correlation analysis was performed using UALCAN data. Kaplan-Meier Plotter database was used for survival analysis. Pathology section analysis was performed using the THPA database. We used Western blot to Verify in vitro experiments Finally, transcription factor prediction and cell experiments were performed. A total of 5 hub genes regulating ferroptosis and interfering LIHC were screened out. Three core genes, AKR1C3, AKR1C1 and AURKA, were selected through gene differential expression. Further analysis found that AURKA was significantly correlated with LIHC survival analysis, clinical parameters and pathological results. We also verified that AURKA protein expression was up-regulated in M.bealei containing serum group compared with HepG2 hepatocellular carcinoma group by Western blot assay(P<0.05). Through the above research, we believe that the M.bealei can intervene in LIHC by acting on AURKA to regulate ferroptosis.

Establishment and application of polysaccharide characteristic degradation profile for Yupingfeng oral liquid

MA Mengnan,ZHANG Yu,LI Xinke,LIU Jinbao,LIU Dan,GUO Huaizhong

2023, 43(6):  616-623.  DOI: 10.3969/j.issn.1000-1565.2023.06.007

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The characteristic degradation profile of Yupingfeng oral liquid polysaccharide(YPF-P)was established in this paper, and the quality control of this preparation was improved based on the profile. YPF-P was obtained by the precipitation of 70% ethanol solution, and the excipient sucrose in the oral liquid was removed in the meantime. The optimized partial degradation conditions of YPF-P were as follows: 0.5 mol/L hydrochloric acid solution, temperature 80 ℃, time 4 h. The degradation products derived by 1-phenyl-3-methyl-5-pyrazolinone(PMP)were separated by capillary zone electrophoresis(CZE)under the following conditions: uncoated fused silica capillary column(50 cm×50 μm i.d., effective length 41 cm)was used, and the running buffer was 40 mmol/L borax buffer(pH 10.1), detection wavelength was set at 245 nm with operating voltage 10 kV, gravity injection 15 cm×20 s was employed, and the column temperature was room temperature. According to the L₉(3⁴)orthogonal design,- DOI:10.3969/j.issn.1000-1565.2023.06.007玉屏风口服液多糖特征降解谱的建立和应用马梦楠¹,张钰¹,李欣珂¹,刘进宝¹,刘丹¹,郭怀忠^1,2(1.河北大学 药学院,河北 保定 071002;2.河北省药物质量分析控制重点实验室,河北 保定 071002)摘 要:建立了玉屏风口服液多糖(YPF-P)的特征降解谱,并基于此完善该制剂的质量控制,通过体积分数70%乙醇溶液沉淀得到YPF-P,同时去除口服液中的辅料蔗糖.优化的YPF-P部分降解条件为0.5 mol/L盐酸溶液,温度80 ℃,时间4 h,随后采用毛细管区带电泳法(CZE)对经过1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生后的YPF-P部分降解产物进行测定.CZE条件为未涂层熔融石英毛细管柱(50 cm × 50 μm i.d.,有效长度41 cm),运行缓冲液40 mmol/L硼砂缓冲液(pH 10.1),检测波长245 nm,运行电压10 kV,重力进样15 cm × 20 s,柱温为室温.根据L₉(3⁴)正交设计组合不同来源的玉屏风处方药材,制备9份玉屏风模拟样品,采用中位数法综合各特征降解谱得到YPF-P特征降解谱对照谱图,并对不同批次样品YPF-P特征降解谱进行聚类分析.结果表明:不同来源和组合的YPF-P特征降解谱差异显著,证明多糖特征降解谱可用于YPF-P成分的分析,也可为其他中药复方制剂多糖成分质量控制的深入研究提供参考.关键词:玉屏风口服液;多糖;特征降解谱;毛细管区带电泳法;中药复方制剂中图分类号:R917 文献标志码:A 文章编号:1000-1565(2023)06-0616-08Establishment and application of polysaccharide characteristic degradation profile for Yupingfeng oral liquidMA Mengnan¹,ZHANG Yu¹,LI Xinke¹,LIU Jinbao¹,LIU Dan¹,GUO Huaizhong^1,2(1. College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China; 2. Key Lab of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, China)Abstract: The characteristic degradation profile of Yupingfeng oral liquid polysaccharide(YPF-P)was established in this paper, and the quality control of this preparation was improved based on the profile. YPF-P was obtained by the precipitation of 70% ethanol solution, and the excipient sucrose in the oral liquid was removed in the meantime. The optimized partial degradation conditions of YPF-P were as follows: 0.5 mol/L hydrochloric acid solution, temperature 80 ℃, time 4 h. The degradation products derived by 1-phenyl-3-methyl-5-pyrazolinone(PMP)were separated by capillary zone electrophoresis(CZE)under the following conditions: uncoated fused silica capillary column(50 cm×50 μm i.d., effective length 41 cm)was used, and the running buffer was 40 mmol/L borax buffer(pH 10.1), detection wavelength was set at 245 nm with operating voltage 10 kV, gravity injection 15 cm×20 s was employed, and the column temperature was room temperature. According to the L₉(3⁴)orthogonal design,- 收稿日期:2023-01-17 基金项目:河北省自然科学基金资助项目(H2021201018;H2016201221);河北省创新能力计划项目(20567605H) 第一作者:马梦楠(1996—),女,河北石家庄人,河北大学在读硕士研究生.E-mail:mmn_0721@163.com 通信作者:郭怀忠(1968—),男,内蒙古商都人,河北大学教授,博士,主要从事中药质量控制及其药效学方向研究.E-mail: ghuaizh@aliyun.com第6期马梦楠等:玉屏风口服液多糖特征降解谱的建立和应用nine Yupingfeng simulated samples were prepared by combining the prescription medicinal materials with different sources of Yupingfeng. The reference YPF-P characteristic degradation profile was obtained by synthesizing the above nine profiles using median method, and the cluster analysis of the characteristic degradation profiles of YPF-P from different batches was carried on. The results indicated that the characteristic degradation profiles of YPF-P from different batches and simulated samples were significantly different, which proved that the characteristic degradation profiles of polysaccharide could be used for the analysis of YPF-P, and could also provide reference for the in-depth study of the quality control of polysaccharide in other Chinese herbal medicine compound preparations.

Effects of long-term continuous 9.375 GHz microwave radiation on Caenorhabditis elegans

ZHANG Wenli, LI Zhihui, DONG Guofu, WEI Liya, WANG Changzhen

2023, 43(6):  624-630.  DOI: 10.3969/j.issn.1000-1565.2023.06.0089.375

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The Caenorhabditis elegans was used to explore the effects of 9.375 GHz microwave radiation on the motor ability, reproductive ability and lifespan of Caenorhabditis elegans. The wild-type strair(N2)and sod-3 mutant(gk235)strain of C. elegans were cultured to adulthood and synchronized. The worms were randomly divided into control and radiation groups((1 000±100)worms per group)when- DOI:10.3969/j.issn.1000-1565.2023.06.0089.375 GHz微波长期辐射对秀丽线虫的影响张文丽^1,2,李志慧²,董国福²,魏丽亚¹,王长振^1,2(1.河北大学 生命科学学院,河北 保定 071002;2.军事医学研究院 辐射医学研究所,北京 100850)摘 要:利用模式生物秀丽线虫(Caenorhabditis elegans)探究9.375 GHz微波辐射对其运动能力、生殖能力和寿命的影响.将野生型株系(N2)和sod-3基因突变株系(gk235)秀丽线虫培养至成年期进行同步化处理,同步化后秀丽线虫长至L1期时,随机分为对照组和辐射组,每组(1 000±100)只,对辐射组进行连续不间断辐射(频率9.375 GHz,比吸收率(specific absorption ratio,SAR)4 W/kg,辐射时间24 h/d),直到秀丽线虫寿命结束为止.当辐射到L4期时,定期统计产卵量;辐射第7天和第10天,分别检测各组秀丽线虫的运动能力(头部摆动、身体弯曲和咽部震动次数)和存活时间.结果表明:辐射第7天,与对照组相比,N2株系秀丽线虫的头部摆动、身体弯曲和咽部震动次数均呈下降趋势,但无统计学差异;辐射第10天,与对照组相比,可显著降低N2株系秀丽线虫的头部摆动次数(P<0.05),而身体弯曲次数和咽部震动次数呈下降趋势,但无统计学差异.与对照组相比,长期辐射可显著降低sod-3基因突变株系秀丽线虫的头部摆动次数和身体弯曲次数(P<0.05),而咽部震动次数无统计学差异.与对照组相比,长期辐射对N2 和sod-3基因突变株系的产卵量和寿命均无明显影响.综上,在频率9.375 GHz、SAR 4 W/kg的条件下,长期持续辐射可降低秀丽线虫的运动能力,但对秀丽线虫的生殖和寿命无明显影响.本实验为后续深入研究X波段辐射损伤机体的作用机制提供参考.关键词:9.375 GHz微波;秀丽线虫;运动能力;生殖;寿命中图分类号:Q955;O552.424 文献标志码:A 文章编号:1000-1565(2023)06-0624-07Effects of long-term continuous 9.375 GHz microwave radiation on Caenorhabditis elegansZHANG Wenli^1,2, LI Zhihui², DONG Guofu², WEI Liya¹, WANG Changzhen^1,2(1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China)Abstract: The Caenorhabditis elegans was used to explore the effects of 9.375 GHz microwave radiation on the motor ability, reproductive ability and lifespan of Caenorhabditis elegans. The wild-type strair(N2)and sod-3 mutant(gk235)strain of C. elegans were cultured to adulthood and synchronized. The worms were randomly divided into control and radiation groups((1 000±100)worms per group)when- 收稿日期:2022-10-21 基金项目:国家自然科学基金资助项目(31800053) 第一作者:张文丽(1997—),女,湖北枣阳人,河北大学在读硕士研究生,主要从事电磁辐射生物效应研究.E-mail:17343355261@163.com 通信作者:魏丽亚(1983—),女,河北石家庄人,河北大学副教授,主要从事生态基因组方向研究.E-mail: weiliya@hbu.edu.cn王长振(1976—),男,山东济宁人,军事医学研究院副研究员,主要从事电磁辐射生物效应方向研究.E-mail: wangcz2002@aliyun.com第6期张文丽等:9.375 GHz微波长期辐射对秀丽线虫的影响they hatched to the L1 phase and started continuous uninterrupted radiation(radio frequency is 9.375 GHz, specific absorption rate(SAR)is 4 W/kg, radiation time is 24 h/d)until the end of worms life. When C. elegans was radiated to the L4 stage, the number of eggs was counted regularly every day. At the time points on the 7th and 10th day of radiation, the motor ability(the number of head swings, body bends, pharyngeal pump vibrations)and survival time of C. elegans in each group were measured, respectively. The results showed that after the 7th day of radiation, the number of head swings, body bends and pharyngeal pump vibrations of the N2 strain showed a decreasing trend compared to the control group, but there was no statistical difference. After the 10th day of radiation, the number of head swings of the N2 strain was significantly reduced compared to the control(P<0.05), while the number of body bends and pharyngeal vibrations tended to decrease, but they were not statistically different. Long-term radiation significantly reduced the number of head swings and body bends in the sod-3 mutant strain compared to the control group(P<0.05), while the number of pharyngeal vibrations was not statistically different. No significant effect on egg production and lifespan of the N2 and sod-3 mutant strains compared to the control group after radiation was observed. In conclusion, under the condition 9.375 GHz and radiation dose of 4 W/kg, long-term continuous radiation can significantly reduce the motor ability of C. elegans, but it has no significant effect on the reproduction and lifespan of C. elegans. This experiment provides a reference for further study of the mechanism of the X-band radiation damage to the organism.

Effect of sacubitril valsartan sodium on cardiomyocyte apoptosis by inhibiting Bax/caspase-3 pathway

LI Yaqin, LIU Yi,WANG Xiaowei, CUI Honggen,KANG Shixin,XU Zhanwen

2023, 43(6):  631-637.  DOI: 10.3969/j.issn.1000-1565.2023.06.009

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To explore the mechanism of sacubitril valsartan sodium(LCZ696)on myocardial apoptosis with heart failure, a heart failure model of SD rats was established by intraperitoneal injection of doxorubicin. After successful model building, rats were randomly divided into HF group, valsartan group, and LCZ696 low(LCZ-L)group, LCZ696 high group(LCZ-H),and control group. After 8 weeks of treatment, the change of MAP, weight and HR were observed. The serum contents of BNP, CK-MB,CK, AST and ALT were detected. The improvement of cardiac function in each group was evaluated by ultrasound. Rat left ventricular myocardial tissue was subjected to HE and Tunel staining; the expression of- DOI:10.3969/j.issn.1000-1565.2023.06.009沙库巴曲缬沙坦钠通过Bax/caspase-3信号通路抑制心肌细胞凋亡李亚芹,刘毅,王潇伟,崔红根,康世鑫,徐占稳(河北大学附属医院 心血管内科,河北 保定 071000)摘 要:为了探索沙库巴曲缬沙坦钠(LCZ696)抑制心衰心肌细胞凋亡的作用机制,腹腔注射盐酸阿霉素构建SD大鼠心衰模型,模型构建成功后随机分为心衰组(HF)、缬沙坦组(Val)、LCZ696低剂量组(LCZ-L)、LCZ696高剂量组(LCZ-H),空白组(Control),治疗8周后观察大鼠平均动脉压(MAP)、体质量、心率(HR)变化,检测血清中脑钠肽(BNP)、肌酸激酶同工酶(CK-MB)、肌酸肌酶(CK)、谷草转氨酶(AST)、谷丙转氨酶(ALT)含量,超声评价各组大鼠心功能改善情况;将大鼠左心室心肌组织进行 HE染色和Tunel染色;检测活化的AKT(P-AKT)、Bax、caspase-3蛋白表达.结果显示:心衰大鼠经Val及LCZ696治疗后超声显示心脏功能均有明显改善,BNP、CK-MB、CK、AST、ALT水平下降,其中LCZ-H组降低最明显;HE染色及Tunel染色显示经治疗后细胞凋亡数减少,心肌组织中P-AKT含量较HF增加,Bax、caspase-3蛋白含量较HF组降低,LCZ-H组减少最显著.综上可知,LCZ696通过抑制Bax/caspase-3信号通路抑制心肌细胞凋亡,改善心功能.关键词:沙库巴曲缬沙坦钠;Bax/caspase-3信号通路;心衰;心肌细胞凋亡 中图分类号:Q952 文献标志码:A 文章编号:1000-1565(2023)06-0631-07Effect of sacubitril valsartan sodium on cardiomyocyte apoptosis by inhibiting Bax/caspase-3 pathwayLI Yaqin, LIU Yi,WANG Xiaowei, CUI Honggen,KANG Shixin,XU Zhanwen(Department of Cardiovascular Medicine, Affiliated Hospital of Hebei University, Baoding 071002,China)Abstract: To explore the mechanism of sacubitril valsartan sodium(LCZ696)on myocardial apoptosis with heart failure, a heart failure model of SD rats was established by intraperitoneal injection of doxorubicin. After successful model building, rats were randomly divided into HF group, valsartan group, and LCZ696 low(LCZ-L)group, LCZ696 high group(LCZ-H),and control group. After 8 weeks of treatment, the change of MAP, weight and HR were observed. The serum contents of BNP, CK-MB,CK, AST and ALT were detected. The improvement of cardiac function in each group was evaluated by ultrasound. Rat left ventricular myocardial tissue was subjected to HE and Tunel staining; the expression of- 收稿日期:2023-06-19 基金项目:河北省卫健委医学科学研究项目(20200203) 第一作者:李亚芹(1973—),女,河北定州人,河北大学附属医院副教授/副主任医师,主要从事冠心病、心力衰竭疾病研究.E-mail:liyqin2014@163.com 通信作者:刘毅(1988—),男,河北安国人,河北大学附属医院主治医师,主要从事冠心病、心衰诊治及介入治疗研究.E-mail:824516368@qq.com王潇伟(1988—),女,河北保定人,河北大学附属医院主治医师,主要从事冠心病及心衰治疗研究.E-mail:775723357@qq.com第6期李亚芹等:沙库巴曲缬沙坦钠通过Bax/caspase-3信号通路抑制心肌细胞凋亡P-AKT, Bax, and caspase-3 proteins was detected. The results showed that heart function improvement after valsartan and LCZ696 treatment.The BNP, CK-MB, CK, AST, and ALT levels were decreased. The most significant reduction was observed in the LCZ-H group. HE and Tunel staining showed decreased myocardial collagen content and apoptosis number after treatment. P-AKT in myocardial tissue increased compared with HF group, Bax and caspase-3 protein decreased compared with HF group, and LCZ-H decreased most significantly. The study show that LCZ696 inhibited cardiomyocyte apoptosis and improved cardiac function by inhibiting the Bax/caspase-3 signaling pathway.

Detection of 2-(trifluoromethyl)phenothiazine in pork by Ic- ELISA

LIU Guangxing,HAN Xiaoli,LI Yiqing,WANG Tingxin

2023, 43(6):  638-645.  DOI: 10.3969/j.issn.1000-1565.2023.06.010

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In order to quickly analyze the sedative 2-(trifluoromethyl)phenothiazine(TFPTZ)in pork, haptens and complete antigens were synthesized using the azobenzoic acid method and the mixed anhydride method, respectively. Polyclonal antibodies were obtained by immunizing New Zealand white rabbits. Indirect competitive enzyme linked immunosorbent assay(Ic-ELISA)was used to quantitatively analyze TFPTZ in pork. The experimental conditions were as follows: the concentration of the coated antigen was 1.25 μg/mL, and the polyclonal antibody was diluted 16 000 times, Coated at 37 ℃ for 3 h, the blocking solution was 1% BSA of mass fraction, and the TFPTZ diluent is PBS of DMSO with a volume fraction of 10%. The competitive reaction lasted 60 minutes. The correlation coefficient R² of this method was 0.999 1, and the IC₅₀ was 1.500 2 μg/L, minimum detection limit IC₁₀ is 0.284 1 μg/L, linear range(IC₂₀~IC₈₀)0.430 7~5.225 2 μg/L, the cross reaction rate with five TFPTZ structural analogues is less than 1.2%, the sample addition recovery rate is 93.26%~109.13%, addition recovery rate is 93.26%~109.13%,- DOI:10.3969/j.issn.1000-1565.2023.06.010猪肉中2-(三氟甲基)吩噻嗪的Ic-ELISA检测刘广兴,韩晓利,李昳晴,王庭欣(河北大学 质量技术监督学院,计量仪器与系统国家地方联合工程研究中心,河北省能源计量与安全检测技术重点实验室(筹),河北 保定 071002)摘 要:为了快速检测猪肉中的镇静剂2-(三氟甲基)吩噻嗪(2-(trifluoromethyl)phenothiazine,TFPTZ),分别用偶氮苯甲酸法、混合酸酐法合成了半抗原、完全抗原,通过免疫新西兰大耳白兔获得了多克隆抗体,采用间接竞争酶联免疫方法(Ic-ELISA)对猪肉中TFPTZ进行了定量分析,实验条件:包被抗原浓度1.25 μg/mL,多克隆抗体稀释16 000倍,37 ℃包被3 h,封闭液为质量分数1%的牛血清白蛋白(BSA),TFPTZ稀释液为含体积分数10%的DMSO的PBS,竞争反应60 min.该方法相关系数R²为0.999 1,IC₅₀为1.500 2 μg/L,最低检测限IC₁₀为0.284 1 μg/L,线性(IC₂₀~IC₈₀)为0.430 7~5.225 2 μg/L,与5种TFPTZ结构类似物的交叉反应率小于1.2%,样品添加回收率为93.26%~109.13%,变异系数小于6%,实际样品检测结果与高效液相色谱法测定结果高度一致,表明建立的快速检测猪肉中TFPTZ的Ic-ELISA方法具有良好的准确度,适用于快速检测猪肉中TFPTZ的残留量.关键词:2-(三氟甲基)吩噻嗪;抗原合成;间接竞争酶联免疫方法(Ic-ELISA)中图分类号:Q5-33 文献标志码:A 文章编号:1000-1565(2023)06-0638-08Detection of 2-(trifluoromethyl)phenothiazine in pork by Ic- ELISALIU Guangxing,HAN Xiaoli,LI Yiqing,WANG Tingxin(Hebei Key Laboratory of Energy Metering and Safety Testing Technology, National & Local Joint Engineering Research Center of Metrology Instrument and System, College of Quality and Technical Supervision, Hebei University, Baoding 071002,China)Abstract: In order to quickly analyze the sedative 2-(trifluoromethyl)phenothiazine(TFPTZ)in pork, haptens and complete antigens were synthesized using the azobenzoic acid method and the mixed anhydride method, respectively. Polyclonal antibodies were obtained by immunizing New Zealand white rabbits. Indirect competitive enzyme linked immunosorbent assay(Ic-ELISA)was used to quantitatively analyze TFPTZ in pork. The experimental conditions were as follows: the concentration of the coated antigen was 1.25 μg/mL, and the polyclonal antibody was diluted 16 000 times, Coated at 37 ℃ for 3 h, the blocking solution was 1% BSA of mass fraction, and the TFPTZ diluent is PBS of DMSO with a volume fraction of 10%. The competitive reaction lasted 60 minutes. The correlation coefficient R² of this method was 0.999 1, and the IC₅₀ was 1.500 2 μg/L, minimum detection limit IC₁₀ is 0.284 1 μg/L, linear range(IC₂₀~IC₈₀)0.430 7~5.225 2 μg/L, the cross reaction rate with five TFPTZ structural analogues is less than 1.2%, the sample addition recovery rate is 93.26%~109.13%, addition recovery rate is 93.26%~109.13%,- 收稿日期:2022-12-18 基金项目:河北省重点研发计划项目(18275502D) 第一作者:刘广兴(1995—),男,河北衡水人,河北大学在读硕士研究生,主要从事食品安全快速检测技术研究.E-mail:603658113@qq.com 通信作者:王庭欣(1965—),女,河北保定人,河北大学教授,主要从事食品安全快速检测技术研究.E-mail:tingxinwang@126.com第6期刘广兴等:猪肉中2-(三氟甲基)吩噻嗪的Ic-ELISA检测and the coefficient of variation is less than 6%. The actual sample detection results are highly consistent with the results determined by high-performance liquid chromatography, indicating that the established Ic-ELISA detection method of TFPTZ has good accuracy and is suitable for rapid detection of TFPTZ residues in pork.

Isolation, identification and dynamic growth characteristics of DSE in Potentilla supina

WU Yanqing, BAI Jinghua, LIU Tiantian

2023, 43(6):  646-652.  DOI: 10.3969/j.issn.1000-1565.2023.06.011

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In order to clarify the relationship between the medicinal and edible plant Potentilla supina with dark septate endophytes(DSE), acid fuchsin staining and direct microscopy were used to observe the colonization of DSE in the roots of P. supina. By DSE isolation and purification by conventional tissue isolation methods, DSE identification by morphology and molecular biology technique, DSE dynamic growth characteristics by plate and liquid culture was elaborated. The results showed that DSE strains colonized the roots of P. supina, the colonization rates of hyphae and microsclerotia were 59.2% and 10.4%, respectively. Nineteen endophytic fungus were isolated from the roots, including nine strains of DSE, which was the dominant population of endophytic fungi in P. supina. Among them, CTW-9 strain was identified as Alternaria alternata Keissler by morphological observation and rDNA-ITS analysis, its GeneBank accession number is MT451845. CTW-9 strain could grow and complete its life cycle normally in both PDA plate and PDB medium, in which, hyphae often could form Hartig net and vesicle-like structures. In conclusion, there were a high colonization frequency of DSE in P. supina plant roots, and Hartig net and vesicle-like structures formed by CTW-9 strain hyphae might be helpful to absorb water and- DOI:10.3969/j.issn.1000-1565.2023.06.011朝天委陵菜DSE的分离鉴定及动态生长特性吴艳清,白菁华,刘沺沺(保定学院 生物化工与环境工程学院,河北 保定 071002)摘 要:为明确药食两用植物朝天委陵菜(Potentilla supina)与深色有隔内生真菌(dark septate endophytes,DSE)之间的关系,以酸性品红染色和直接镜检观察朝天委陵菜根内DSE的定殖情况,采用常规组织分离方法分离DSE菌株并纯化培养,利用形态学和分子生物学相结合的方法鉴定DSE菌株,通过平板和液体培养了解DSE菌株的动态生长特性.结果显示:朝天委陵菜根内有DSE菌株定殖,菌丝和微菌核定殖率分别为59.2%和10.4%;从根部分离得到19株内生真菌,其中包含9株DSE,是朝天委陵菜内生真菌优势种.经形态学观察、比较和rDNA-ITS分析,将9株DSE中的CTW-9菌株鉴定为链格孢(Alternaria alternata),登录号为MT451845,该菌株在PDA平板和PDB培养液中均可正常生长并完成生活史,菌丝能形成哈氏网和类泡囊等结构.综上所述,DSE菌株在朝天委陵菜根部有较高的定殖率,CTW-9菌株的菌丝常形成哈氏网和类泡囊等特殊结构,可能有助于植物从环境中吸收和积累水分及营养物质. 关键词:深色有隔内生真菌(DSE);朝天委陵菜;链格孢;分离纯化;动态生长特性中图分类号:Q954.6 文献标志码:A 文章编号:1000-1565(2023)06-0646-07Isolation, identification and dynamic growth characteristics of DSE in Potentilla supinaWU Yanqing, BAI Jinghua, LIU Tiantian(College of Biochemical and Environmental Engineering, Baoding University, Baoding 071002, China)Abstract: In order to clarify the relationship between the medicinal and edible plant Potentilla supina with dark septate endophytes(DSE), acid fuchsin staining and direct microscopy were used to observe the colonization of DSE in the roots of P. supina. By DSE isolation and purification by conventional tissue isolation methods, DSE identification by morphology and molecular biology technique, DSE dynamic growth characteristics by plate and liquid culture was elaborated. The results showed that DSE strains colonized the roots of P. supina, the colonization rates of hyphae and microsclerotia were 59.2% and 10.4%, respectively. Nineteen endophytic fungus were isolated from the roots, including nine strains of DSE, which was the dominant population of endophytic fungi in P. supina. Among them, CTW-9 strain was identified as Alternaria alternata Keissler by morphological observation and rDNA-ITS analysis, its GeneBank accession number is MT451845. CTW-9 strain could grow and complete its life cycle normally in both PDA plate and PDB medium, in which, hyphae often could form Hartig net and vesicle-like structures. In conclusion, there were a high colonization frequency of DSE in P. supina plant roots, and Hartig net and vesicle-like structures formed by CTW-9 strain hyphae might be helpful to absorb water and- 收稿日期:2022-10-05 基金项目:河北省高等学校科学研究计划项目(QN2021401);河北省应用技术研究会项目(JY2021099) 第一作者:吴艳清(1982—),男,河北河间人,博士,保定学院高级实验师,主要从事微生物资源开发及应用研究.E-mail:wuyanqing@bdu.edu.cn第6期吴艳清等:朝天委陵菜DSE的分离鉴定及动态生长特性nutrition from environment for plants.

A movie recommendation method based on the positive and negative preferences of users with emotional factors

ZHANG Bin, DONG Yaqian, XU Jianmin

2023, 43(6):  653-664.  DOI: 10.3969/j.issn.1000-1565.2023.06.012

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In the existing movie recommendation methods based on users positive and negative preferences. The users sentiment information was not fully considered, the users positive and negative preferences was not accurate enough, and the recommendation effect was not ideal. In this paper, a method is proposed to improve users positive and negative preferences by using users emotions contained in reviews, and the movie recommendation is realized based on the new preferences. Firstly, the sentiment dictionary in the field of movie was constructed, which is used to realize the mining and quantification of the review data and obtain the sentiment score of user reviews. Secondly, the users preference degree for movies was calculated in order to generate the users positive and negative preference movie sets by considering the users rating and the comment sentiment score comprehensively, then according to the two sets, the users positive and negative preference feature vectors were constructed, and the users positive and negative preferences containing user sentiment were mined. Finally, the similarity, which is between the candidate movie and the users positive and negative preference features, was used to calculate the users final rating for the candidate movie, and the movie recommendation was realized. Compared with traditional - DOI:10.3969/j.issn.1000-1565.2023.06.012基于蕴含情感要素用户正负偏好的电影推荐方法张彬,董雅倩,徐建民(河北大学 网络空间安全与计算机学院,河北 保定 071000)摘 要:针对现有基于用户正负偏好的电影推荐方法未充分考虑用户情感信息、用户正负偏好表示不够准确以及推荐效果不够理想的问题,提出一种利用评论中蕴含的用户情感改进其正负偏好,基于改进偏好实现电影推荐的方法.首先,构建电影领域情感词典,利用该词典实现对评论数据的挖掘与量化,得到用户评论的情感得分;然后,综合考虑用户的评分和评论情感得分计算用户对电影的喜好度,从而生成用户的正、负向偏好电影集合,并基于这2个集合构建用户的正、负向偏好特征向量,挖掘得到蕴含评论情感的用户正负偏好;最后,利用候选电影与用户正、负向偏好特征的相似度计算用户对候选电影的最终评分,实现电影推荐.基于豆瓣数据集的实验结果表明,本文方法的各项指标相对于传统方法有一定提升,其中F1、MAE和MAPE指标分别提升6.10%、3.32%、11.67%.关键词:电影推荐;正向偏好;负向偏好;评论情感中图分类号:TP391 文献标志码:A 文章编号:1000-1565(2023)06-0653-12A movie recommendation method based on the positive and negative preferences of users with emotional factorsZHANG Bin, DONG Yaqian, XU Jianmin(School of Cyber Security and Computer, Hebei University, Baoding 071000, China)Abstract: In the existing movie recommendation methods based on users positive and negative preferences. The users sentiment information was not fully considered, the users positive and negative preferences was not accurate enough, and the recommendation effect was not ideal. In this paper, a method is proposed to improve users positive and negative preferences by using users emotions contained in reviews, and the movie recommendation is realized based on the new preferences. Firstly, the sentiment dictionary in the field of movie was constructed, which is used to realize the mining and quantification of the review data and obtain the sentiment score of user reviews. Secondly, the users preference degree for movies was calculated in order to generate the users positive and negative preference movie sets by considering the users rating and the comment sentiment score comprehensively, then according to the two sets, the users positive and negative preference feature vectors were constructed, and the users positive and negative preferences containing user sentiment were mined. Finally, the similarity, which is between the candidate movie and the users positive and negative preference features, was used to calculate the users final rating for the candidate movie, and the movie recommendation was realized. Compared with traditional - 收稿日期:2023-02-23 基金项目:河北省社会科学基金资助项目(HB21TQ005) 第一作者:张彬(1980—),男,河北涿州人,河北大学高级实验师,博士,主要从事信息检索方向研究.E-mail:zb@hbu.edu.cn 通信作者:徐建民(1966—),男,河北馆陶人,河北大学教授,博士生导师,主要从事信息检索方向研究.E-mail:hbuxjm@hbu.edu.cn第6期张彬等:基于蕴含情感要素用户正负偏好的电影推荐方法methods, the experimental results based on Douban dataset show that the proposed method has certain improvements over the traditional methods, and the indexes of F1, MAE and MAPE are improved by 6.10%, 3.32% and 11.67%, respectively.

An improved KCF mixed with deep feature for pedestrian tracking

CHEN Xiangyang, ZHOU Yang, YANG Wenzhu

2023, 43(6):  665-672.  DOI: 10.3969/j.issn.1000-1565.2023.06.013

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The KCF(kernel correlation filter)algorithm is currently one of the best tracking algorithms, but it has no scale adaptability and cannot solve the occlusion problem. To solve the problems of the KCF algorithm, an improved KCF model, named IKCFMDF(improved KCF mixed with deep feature), is proposed. This model obtains the prediction frame of the target pedestrian by the KCF algorithm, and then compares it with all the pedestrian detection frames obtained through target detection method, and uses the detection frame with the largest intersection-over-union as the new training target, thus achieving the scale adaptation of KCF. By introducing the deep features instead of the HOG features of KCF,the algorithm can avoid the defects that HOG(histogram of oriented gradient)features are insensitive to pose changes and color information of pedestrians. The deep feature is retained when the tracking target is lost, and then the deep feature is compared with the pedestrians in the subsequent videos. If the similarity is greater than the set threshold, it can determine that the target reappears, thereby the occlusion problem of KCF is solved. Compared with the original KCF, the success rate of pedestrian tracking in complex situations is increased by 36%, and compared with the popular neural network models, the proposed model also achieves improvement.- DOI:10.3969/j.issn.1000-1565.2023.06.013融合深度特征的改进KCF行人追踪算法陈向阳,周扬,杨文柱(河北大学 网络空间安全与计算机学院,河北机器视觉工程研究中心,河北 保定 071000)摘 要:KCF(kernel correlation filter)算法是目前追踪效果最好的算法之一,但KCF算法无尺度自适应能力且不能有效解决遮挡问题.针对KCF算法的缺陷,设计了1种改进的KCF模型,IKCFMDF(improved KCF mixed with deep feature).该模型先由KCF算法得到目标行人的预测框,然后与通过目标检测得到的所有行人检测框相比较,将交并比最大的检测框作为新的训练目标,从而实现KCF的尺度自适应;引入深度特征代替原KCF使用的HOG(histogram of oriented gradient)特征,以避免HOG特征对行人的姿势改变和颜色信息不敏感的缺陷;在追踪目标丢失时保留其深度特征,与之后视频中行人的深度特征进行对比,如果相似度大于设定阈值,则可判定目标重新出现,从而有效解决KCF的遮挡问题.与原始KCF相比,改进的模型在复杂情况下,对行人追踪的成功率提升了36%,相比主流的神经网络模型也有少量的提升.关键词:行人追踪;改进的KCF模型;深度特征;目标检测中图分类号:TP391 文献标志码:A 文章编号:1000-1565(2023)06-0665-08An improved KCF mixed with deep feature for pedestrian trackingCHEN Xiangyang, ZHOU Yang, YANG Wenzhu(Hebei Machine Vision Engineering Research Center, School of Cyber Security and Computer, Hebei University, Baoding 071000, China)Abstract: The KCF(kernel correlation filter)algorithm is currently one of the best tracking algorithms, but it has no scale adaptability and cannot solve the occlusion problem. To solve the problems of the KCF algorithm, an improved KCF model, named IKCFMDF(improved KCF mixed with deep feature), is proposed. This model obtains the prediction frame of the target pedestrian by the KCF algorithm, and then compares it with all the pedestrian detection frames obtained through target detection method, and uses the detection frame with the largest intersection-over-union as the new training target, thus achieving the scale adaptation of KCF. By introducing the deep features instead of the HOG features of KCF,the algorithm can avoid the defects that HOG(histogram of oriented gradient)features are insensitive to pose changes and color information of pedestrians. The deep feature is retained when the tracking target is lost, and then the deep feature is compared with the pedestrians in the subsequent videos. If the similarity is greater than the set threshold, it can determine that the target reappears, thereby the occlusion problem of KCF is solved. Compared with the original KCF, the success rate of pedestrian tracking in complex situations is increased by 36%, and compared with the popular neural network models, the proposed model also achieves improvement.- 收稿日期:2022-03-25 基金项目:河北省自然科学基金资助项目(F2018201060) 第一作者:陈向阳(1977—),女,河南三门峡人,河北大学讲师,主要从事机器视觉方向研究.E-mail:chenxyhbu@163.com 通信作者:杨文柱(1968—),男,河北保定人,河北大学教授,博士,主要从事机器视觉方向研究.E-mail:wenzhuyang@163.com第6期陈向阳等:融合深度特征的改进KCF行人追踪算法