Molecular identification of crab spiders from Xiaowutai Mountains,China with DNA barcoding

doi:10.3969/j.issn.1000-1565.2016.03.011

Abstract

Abstract: In order to explore the feasibility of DNA barcoding in the species identification of crab spiders,the COI gene of 110 thomisid samples belonged to 25 species in Xiaowutai Mountains,China,was applied; the phylogenetic trees were reconstructed by neighbor-joining(NJ)and Bayesian Inference(BI)methods,and with ABGD(automatic barcode gap discovery)software to divide the samples.The results showed that the samples belonging to the same species clustered in one monophyly and all species were successfully distinguished by the phylogenetic trees,and the groups automatically defined by ABGD perfectly matched the groups defined by morphological characters,and accorded with phylogenetic trees.We believe DNA barcoding can be used as an effective tool in the molecular identification of crab spiders.

Key words: Thomisidae, DNA barcoding, phylogenetic trees, ABGD

CLC Number:

Q959.2262

HE Jingchao,HU Lanlan,GUO Chenhui,ZHANG Feng. Molecular identification of crab spiders from Xiaowutai Mountains,China with DNA barcoding[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(3): 286-292.

References

[1] 程希婷,王爱民,顾志峰,等.DNA条形码研究进展[J].基因组学与应用生物学, 2011,30(6):748-758. CHENG Xiting,WANG Aimin,GU Zhifeng,et al.Current progress of DNA barcoding[J].Genomics and Applied Biology,2011,30(6):748-758.
[2] 肖金花,肖晖,黄大卫.生物分类学的新动向——DNA条形编码[J].动物学报,2004,50(5):852-855. XIAO Jinhua,XIAO Hui,HUANG Dawei.DNA barcoding:new approach of biological taxonomy[J].Acta Zoologica Sinica,2004,50(5):852-855.
[3] HEBERT P D N,CYWINSKA A,BALL S L,et al.Biological identifications through DNA barcodes[J].Proceedings of the Royal Society B:Biological Sciences,2003,270:313-321.DOI:10.1098/rspb.2002.2218.
[4] HEBERT P D N,RATNASINGHAM S,DEWAARD J R.Barcoding animal life:cytochrome c oxidase subunit 1 divergences among closely related species[J].Proceedings of the Royal Society B:Biological Sciences,2003,270:S96-S99.DOI:10.1098/rsbl.2003.0025.
[5] KWONG S,SRIVATHSAN A,MEIER R.An update on DNA barcoding:low species coverage and numerous unidentified sequences[J].Cladistics,2012,28:639-644.DOI:10.1111/j.1096-0031.2012.00408.
[6] HEBERT P D N,STOECKLE M Y,ZEMLAK T S,et al.Identification of birds through DNA barcodes[J].PLoS Biology,2004,2:1657-1663.DOI:10.1371/journal.pbio.0020312.
[7] WARD R D,ZEMLAK T S,INNES B H,et al.DNA barcoding Australia’s fish species[J].Proceedings of the Royal Society B:Biological Sciences,2005,360:1847-1857.DOI:10.1001/archneurol.2008.523.
[8] HAJIBABAEI M,JANZEN D H,BURNS J M,et al.DNA barcodes distinguish species of tropical Lepidoptera[J].Proceedings of the National Academy of Sciences,USA,2006,103:968-971.DOI:10.1073/pnas.0510466103.
[9] CRAFT K J,PAULS S U,DARROW K,et al.Population genetics of ecological communities with DNA barcodes:an example from New Guinea Lepidoptera[J].Proceedings of the National Academy of Sciences,USA,2010,107:5041-5046.DOI:10.1073/pnas.0913084107.
[10] MURPHY R W,CRAWFORD A J,BAUER A M,et al.Cold Code:the global initiative to DNA barcode amphibians and nonavian reptiles[J].Molecular Ecology Resources,2013,13:161-167.DOI:10.1111/1755-0998.12050.
[11] CHE J,CHEN H M,YANG J X,et al.Universal COI primers for DNA barcoding amphibians[J].Molecular Ecology Resources,2012,12:247-258.DOI:10.1111/j.1755-0998.2011.03090.x.
[12] 何锴,王文智,李权,等.DNA条形码技术在小型兽类鉴定中的探索:以甘肃莲花山为例[J].生物多样性,2013,21(2):197-205. HE Kai,WANG Wenzhi,LI Quan,et al.DNA barcoding in surveys of small mammal community:a case study in Lianhuashan,Gansu Province,China[J].Biodiversity Science,2013,21(2):197-205.
[13] 李超伦,王敏晓,程方平,等.DNA条形码及其在海洋浮游动物生态学研究中的应用[J].生物多样性,2011,19(6):805-814. LI Chaolun,WANG Minxiao,CHENG Fangping,et al.DNA barcoding and its application to marine zooplankton ecology[J].Biodiversity Science,2011,19(6):805-814.
[14] ARDURA A,POLA I G,GINUNO I,et al.Application of barcoding to Amazonian commercial fish labeling[J].Food Research International,2010,43(5):1549-1552.DOI:10.1016/j.foodres.2010.03.016.
[15] WILL K W,MISHLER B D,WHEELER Q D.The perils of DNA barcoding and the need for integrative taxonomy[J].Syst Biol,2005,54(5),844-851.DOI:10.1080/10635150500354878.
[16] MORITZ C.CICERO C,DNA barcoding:Promise and pitfalls[J].Plos Biology,2004,2(10):e354.DOI:10.1371/journal.pbio.0020354.
[17] SPERLING F.DNA Barcoding:Deusex machina[J].Society,2003,45(4):1-4.
[18] World Spider Catalog(2015).World Spider Catalog[J/OL].Version 16.0.Natural History Museum Bern.[2015-01-22] ,http://wsc.nmbe.ch.
[19] FOLMER O,BLACK M,HOEH W,et al.DNA primer for amplification of mitochondrial cyctorome coxidase subunit I from diverse metazoan invertebrates[J].Mol Mar Biotechnol,1994,3(5):294-199.
[20] THOMPSON J D,GIBSON T J,PLEWNIAK F,et al.The CLUSTAL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Research,1997,25(24):4876-4882.DOI:10.1093/nar/25.24.4876.
[21] TAMURA K,STECHER G,PETERSON D,et al.MEGA6:molecular evolutionary genetics analysis version 6.0.[J].Molecular Biology and Evolution,2013,30:2725-2729.DOI:10.1093/molbev/mst197.
[22] KIMURE M A.Simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences[J].Journal of Molecular Evolution,1980,16:111-120.DOI:10.1007/BF01731581.
[23] HUELSENBECK J P,RONQUIST F.MrBayes:Bayesian inference of phylogenetic trees[J].Bioinformatics,2001,17:754-755.DOI:10.1093/bioinformatics/17.8.754.
[24] MEYER C P,PAULAY G.DNA barcoding:error rates based on comprehensive sampling[J].PLoS Biology,2005,3(12):e422.DOI:10.1371/journal.pbio.0030422.
[25] PUILLANDRE N,LAMBERT A,BROUILLET S,et al.ABGD,automatic barcode gap discovery for primary species delimitation[J].Mol Ecol,2011,21(8):1864-1877.DOI:10.1111/j.1365-294X.2011.05239.x.
[26] ZHANG Y Y,LI S Q.A spider species complex revealed high cryptic diversity in South China caves[J].Molecular Phylogenetics and Evolution,2014,79:353-358.DOI:10.1016/j.ympev.2014.05.017.
[27] BERNHARD A,JONAS J.Increased samp ling blurs morpho logical and molecular species limits:revision of the Hispanio lan endemic spider genus Tainonia(Araneae:Pholcidae)[J].Astrin Invertebrate Systematics,2009,23:281-300.DOI:10.1071/IS09017.
[28] FRANZINI P Z N.Combined analyses of genetic and morphological data indicate more than one species of Cyrtophora(Araneae:Araneidae)in South Africa[J].Internationnal Journal of Modern Biological Research 2013,1:21-34.
[29] ZHANG J X,MADDISON W P.Molecular phylogeny,divergence times and biogeography of spiders of the subfamily Euophryinae(Araneae:Salticidae)[J].Molecular Phylogenetics and Evolution,2013,68:81-92.DOI:10.1016/j.ympev.
[30] 刘勇,宋毓,李晓宇.基于线粒体COI基因的DNA条形码技术在昆虫分子鉴定中的应用[J].植物检疫,2010,2(24):46-50.
[31] 宁淑萍,颜海飞,郝刚,等.植物DNA条形码研究进展[J].生物多样性,2008,16(5):417-425.DOI:10.3724/SP.J.1003.2008.08215. NING Shuping,YAN Haifei,HAO Gang,et al.Current advances of DNA barcoding study in plants[J].Biodiversity Science,2008,16(5):417-425.DOI:10.3724/SP.J.1003.2008.08215.