Separation and identification of acid proof yeast and its application in fermentation of waste fermenting grains

doi:10.3969/j.issn.1000-1565.2016.05.011

Abstract

Abstract: The two yeaststrainsYP2 and YZ13 were isolated from Chinese-spirit fermenting grains and they could all grow well under the condition of pH 3.0.Strain YP2 was identified as belonging to Candida and the similarity degree of strain YP2 and Candida pseudolambica(KM384061.1)reached 98%,while strain YZ13 to Pichia and the similarity degree with Pichia kudriavzevii(LC014798.1)reached 100%.The two strains were mixed at the ratio of 1∶1 in weight and applied to the fermentation of waste fermenting grains.The process of solid state fermentation lasted for 60 hours under the condition of inoculation quantity 100 mL/kg,fresh fermenting grains 870 g/kg,wheat bran 100 g/kg,ammonia sulfate 15 g/kg,quicklime 15 g/kg,temperature 28 ℃.The results showed that the protein content of the dry sample of waste fermenting grains reached 172.30 g/kg and increased by 28.29% and it enriched feed protein of waste fermenting grains and provided foundation for waste fermenting grains and high efficiency feed.

Key words: yeast, acid proof, true protein

CLC Number:

X172
X797

LI Yanqin,CUI Haihao,LI Chunqing,SONG Ruixue,CHEN Yajun. Separation and identification of acid proof yeast and its application in fermentation of waste fermenting grains[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(5): 517-523.

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