Abstract: In order to study the specific function of protein disulfide isomerase A3 precursor(PDIA3)in T cell receptor(TCR)signaling pathway, the PDIA3 protein level in T cells was knocked down by electroporation, the phosphorylation level of zeta-chain associated protein 70( ZAP70)protein was detected by enzyme linked immunosorbent assay(ELISA), and the expression level of cluster of differentiation 69(CD69)were analyzed by flow cytometry. The luciferase reporter gene detected NF-κB signaling pathway. The results showed that down-regulation of PDIA3 protein in T cells resulted in a significant decrease in ZAP70 protein phosphorylation, CD69 expression, IL-2 secretion, and affected NF-κB signaling pathway, indicating PDIA3 protein promotes the activation of T cells and participate in the regulation of T cell TCR signaling pathway. This work provides an important research basis for studying the interaction between PDIA3 and some functional proteins downstream of TCR in the future.

Key words: protein disulfide isomerase A3 precursor(PDIA3), T cell receptor(TCR), T cells