Response of Trichoderma strains in the rhizosphere of medicinal plants on drought stress

doi:10.3969/j.issn.1000-1565.2021.04.009

Abstract

Abstract: In order to explore the response of drought stress in Trichoderma isolated from the root zone of medicinal plants, seven Trichoderma strains were isolated from the medicinal materials planting base in Anguo, Hebei province, China. The two methods of solid culture and liquid culture were used in this experiment. And PEG-6000 at different mass concentrations(0, 60, 120, 180, 240 g/L)were used to simulate drought stress to study the growth and physiological response of Trichoderma spp. in arid environment. The results showed that the biomass of Trichoderma strains varied under different drought conditions; only the biomass of T. atroviride and T. lixii were higher than that of the control group when the PEG-6000 mass concentration was 60 g/L. While the biomass of T. longibrachiatum was higher than- DOI:10.3969/j.issn.1000-1565.2021.04.009药用植物根区木霉菌对干旱胁迫的响应杨静雅,张开逊,张东东,赵丽莉,贺学礼(河北大学生命科学学院,河北保定 071002)摘要:为探究药用植物根区土壤木霉菌对干旱胁迫的响应,选取了河北安国中药材种植基地分离的7种木霉菌,采用固体培养和液体培养2种方式,通过不同质量浓度PEG-6000(0、60、120、180、240 g/L)模拟干旱胁迫,研究干旱环境对木霉菌生长及生理响应.结果表明,不同干旱程度下,木霉菌种间生物量存在差异.对于Trichoderma atroviride和T. lixii,其生物量仅在PEG-6000质量浓度60 g/L时高于对照组,而T. longibrachiatum生物量除PEG-6000质量浓度120 g/L外,其他PEG-6000质量浓度下生物量均高于对照组.随干旱程度增强,木霉菌可溶性蛋白含量总体呈升高趋势,而谷胱甘肽(GSH)含量(除T. virilente、T. afroharzianum和T. inhamatum)整体呈降低趋势,丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性变化因不同干旱程度和木霉菌种而异.双因素方差分析结果表明,干旱胁迫和木霉种类对木霉菌生长及生理指标均有影响.干旱胁迫显著影响木霉菌可溶性蛋白含量,而木霉菌种主要影响GSH、MDA含量和SOD活性.主成分分析结果表明,MDA和SOD是反映木霉菌对干旱响应的主要指标.综合分析发现,T. afroharzianum、T. atroviride、T. lixii和T. longibrachiatum属于耐旱性强的木霉菌种.本研究为进一步发掘和应用抗旱优良菌种提供依据.关键词:木霉菌;耐旱性;生长指标;生理指标中图分类号:Q969 文献标志码:A 文章编号:1000-1565(2021)04-0393-13Response of Trichoderma strains in the rhizosphere of medicinal plants on drought stressYANG Jingya,ZHANG Kaixun,ZHANG Dongdong,ZHAO Lili, HE Xueli(College of Life Sciences, Hebei University, Baoding 071002, China)Abstract: In order to explore the response of drought stress in Trichoderma isolated from the root zone of medicinal plants, seven Trichoderma strains were isolated from the medicinal materials planting base in Anguo, Hebei province, China. The two methods of solid culture and liquid culture were used in this experiment. And PEG-6000 at different mass concentrations(0, 60, 120, 180, 240 g/L)were used to simulate drought stress to study the growth and physiological response of Trichoderma spp. in arid environment. The results showed that the biomass of Trichoderma strains varied under different drought conditions; only the biomass of T. atroviride and T. lixii were higher than that of the control group when the PEG-6000 mass concentration was 60 g/L. While the biomass of T. longibrachiatum was higher than- 收稿日期:2020-11-02 基金项目:国家自然科学基金资助项目((31770561);河北大学研究生创新项目(hbu2020ss039) 第一作者:杨静雅(1995—),女,河南商丘人,河北大学在读硕士研究生,主要从事菌根生物学方向研究.E-mail: yangjingya196@foxmail.com 通信作者:赵丽莉(1964—),女,陕西富平人,河北大学副教授,主要从事菌根生物学方向研究. E-mail: zhao3615@126.com第4期杨静雅等:药用植物根区木霉菌对干旱胁迫的响应that of the control group at other PEG-6000 mass concentrations except the PEG-6000 mass concentration of 120 g/L. With increasing drought, the soluble protein content of Trichoderma spp. showed an overall increasing trend, but the glutathione content of Trichomonas spp. except T. virilente, T. afroharzianum and T. inhamatum showed an overall decreasing trend. However, the variation of malondialdehyde content and superoxide dismutase activity were highly depended on different levels of stress and Trichoderma species. The results of two-factor analysis of variance indicated that drought stress and Trichoderma species had an impact on the growth and physiological indicators of Trichoderma. Principal component analysis showed that malondialdehyde and superoxide dismutase were the main indicators reflecting the response of Trichoderma to drought. The comprehensive analysis revealed that T. afroharzianum, T. atroviride, T. lixii and T. longibrachiatum had strong drought tolerance. This study provides a basis for the discovery and application of beneficial fungi with the ability of drought resistance.

Key words: Trichoderma, drought tolerance, growth index, physiological index

CLC Number:

Q969

YANG Jingya,ZHANG Kaixun,ZHANG Dongdong,ZHAO Lili, HE Xueli. Response of Trichoderma strains in the rhizosphere of medicinal plants on drought stress[J]. Journal of Hebei University(Natural Science Edition), 2021, 41(4): 393-405.

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