DNA binding and photocleavage properties of a dinuclear ruthenium complex

doi:10.3969/j.issn.1000-1565.2019.03.006

Abstract

Abstract: A dinuclear ruthenium complex has been synthesized and characterized. The calf thymus DNA(ct-DNA)binding properties of the complex are investigated by means of DNA viscosity and optical spectroscopic techniques of UV-visible absorption and emission spectral titrations, DNA thermal denaturation and reverse, salt effect and agarose gel electrophoresis. The results suggest that the complex binds to DNA through partial-intercalation mode with a large DNA binding constant of 10⁶ L/mol order of magnitude. The complex is also demonstrated to be an efficient cleaver of pBR 322 DNA under irradiation at 360 nm.

Key words: ruthenium complex, DNA, bind, electrophoresis

CLC Number:

Q523

WANG Shuang, XU Xuexue, MI Yaxuan, ZHENG Zebao, ZHAO Xiaolong. DNA binding and photocleavage properties of a dinuclear ruthenium complex[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(3): 254-263.

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