Effect of physiological and biochemical indexes on young panicles under chemical hybridization agent SQ-1 in foxtail millet

doi:10.3969/j.issn.1000-1565.2019.04.012

Abstract

Abstract: The physiological-biochemical indexes of the panicle of foxtail millet were studied under the male-gametocide SQ-1.Yugu 1 as a main foxtail millet cultivar was used in this study.The five physiological indexes of the young panicle were determined, including malondialdehyde(MDA)content, electrical conductivity(EC), activity of peroxidase(POD), superoxide dismutase(SOD)activity and catalase(CAT)activity.The results indicated that the young panicle conductivity was significantly increased under SQ-1 and gradually declined in the first day.The fluctuation of malondialdehyde was small and the results showed the trend was first rised and then decreased, however, the maximum was observed at the fifth day.SOD activity- DOI:10.3969/j.issn.1000-1565.2019.04.012化学杀雄剂SQ-1对谷子幼穗生理生化指标的影响王春芳¹,张温典¹,李天聪¹,熊雪¹,谷守芹³,李伟²,史慎奎¹(1.河北民族师范学院生物与食品科学系,河北承德 067000;2.河北农林科学院谷子研究所,河北石家庄 050035;3.河北农业大学生命科学学院,河北保定 071001)摘要:以豫谷1号为实验材料,研究化学杀雄剂杀雄嗪酸(2-(4-氯苯基)-3-乙基-2,5-二氢-5-氧哒嗪-4-羧酸,SQ-1)对谷子幼穗5个典型生理生化指标——丙二醛(MDA)含量、电导率(EC)、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性的影响.研究结果表明:使用SQ-1处理后的幼穗EC第1天达到峰值,随后呈现逐渐下降的趋势;MDA含量波动幅度较小,呈现先上升后降低的趋势,第5天达到最大值;SOD活性呈现先降低后上升再降低的趋势,第4天达到最高点;POD活性呈现先降低后上升的趋势,第7天达到最大值;CAT活性第5天有小幅度下降,总体呈现逐渐上升的趋势.本研究为后续探究SQ-1导致谷子不育的分子机理提供数据支持,也为今后谷子杂交育种奠定理论基础.关键词:杀雄嗪酸;谷子;幼穗;生理生化指标中图分类号:S131 文献标志码:A 文章编号:1000-1565(2019)04-0404-08Effect of physiological and biochemical indexes on young paniclesunder chemical hybridization agent SQ-1 in foxtail milletWANG Chunfang¹, ZHANG Wendian¹, LI Tiancong¹, XIONG Xue¹, GU Shouqin³, LI Wei², SHI Shenkui¹(1.Department of Biological and Food Science, Hebei Normal University for Nationalities, Chengde 067000, China; 2.Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035; China; 3.College of Life Sciences, Hebei Agricultural University, Baoding 071001, China)Abstract: The physiological-biochemical indexes of the panicle of foxtail millet were studied under the male-gametocide SQ-1.Yugu 1 as a main foxtail millet cultivar was used in this study.The five physiological indexes of the young panicle were determined, including malondialdehyde(MDA)content, electrical conductivity(EC), activity of peroxidase(POD), superoxide dismutase(SOD)activity and catalase(CAT)activity.The results indicated that the young panicle conductivity was significantly increased under SQ-1 and gradually declined in the first day.The fluctuation of malondialdehyde was small and the results showed the trend was first rised and then decreased, however, the maximum was observed at the fifth day.SOD activity- 收稿日期:2018-11-01 基金项目:河北省自然科学基金资助项目(C2016101019);河北民族师范学院校级课题(QN201601);河北省高等学校科学技术研究项目(QN2018330) 第一作者:王春芳(1983—),女,河北承德人,满族,河北民族师范学院讲师,主要从事作物分子生物学研究.E-mail:wangchunfang18@126.com 通信作者:李伟(1979—),男,河北石家庄人,河北农林科学院副研究员,主要从事谷子分子生物学研究.E-mail:hbnkylw@126.com史慎奎(1984—),男,河北衡水人,河北民族师范学院讲师,博士,主要从事作物分子生物学研究.E-mail:shishenkui@126.com第4期王春芳等:化学杀雄剂SQ-1对谷子幼穗生理生化指标的影响displayed variation pattern and reached the highest point at the fourth day.POD activity was decreased and then increased and reached the maximum at the seventh day.CAT activity at the fifth day was decreased slightly and the whole trend showed a gradual increase. This study provided data support for subsequent exploring the molecular mechanism of foxtail millet infertility under SQ-1, and would lay a theoretical basis for foxtail millet hybrid breeding in the future.

Key words: SQ-1, foxtail millet, young panicle, physiological-biochemical index

CLC Number:

S131

WANG Chunfang, ZHANG Wendian, LI Tiancong, XIONG Xue, GU Shouqin, LI Wei, SHI Shenkui. Effect of physiological and biochemical indexes on young panicles under chemical hybridization agent SQ-1 in foxtail millet[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(4): 404-411.

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