Abstract: Through the single factor test and the orthogonal test method, the optimal carbon and nitrogen source and the optimal formula for the fermentation of Auricularia auricula the mechanism of extracting the spike of cattail were studied. The fermentation broth was used to homogenize the fermentation broth for the best bacterial age, and was used to culture the test drosophila, and the drosophila life test was carried out to observe the effect on the life span of the drosophila. The results showed that the optimal fermentation formula was: glucose 3%, corn flour 3%, bean cake powder 2%, yeast extract 0.5%, potassium phosphate monobasic 0.1%, magnesium sulfate 0.1%; and when fermented to 120 h, the wet weight of mycelium reached the maximum value of 32.57 g. When 30% fermented mash was added to the drosophila basal medium, the half-life death rate, average life expectancy and maximum life-long life rate of drosophila were the highest, and they were significantly different from the control group(P<0.05)

Key words: Auricularia auricula, the spike of cattail, formula optimization, fermentation mash, drosophila life