Table of Content

25 May 2022, Volume 42 Issue 3

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Bi-objective programming model for location-routing problem in uncertain emergency system

GAO Rong, WANG Chun, MA Yebao

2022, 42(3):  225-231.  DOI: 10.3969/j.issn.1000-1565.2022.03.001

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For improving the efficiency of delivery time and reducing cost for emergency materials distribution after natural disasters, this paper aims to construct a uncertain bi-objective chance-constrained programing model for optimizing total travel time and total cost, where the time restriction of demand center, the candidate rescue centers capacity and the vehicles capacity are limited, and the demand of materials and the travel time between two nodes are uncertain. For saving cost, vehicles are considered to stand at the nearby opened rescue center when it finishes its delivery tasks. In this paper, the programming model is solved by a genetic algorithm and the objective is handled by the idea of penalty function and weighting method. Finally, an numerical example is designed to show that this model and the algorithm are useful and available.

Fuzzy data envelopment analysis without explicit inputs and its applications

JI Aibing, PANG Jiahong

2022, 42(3):  232-240.  DOI: 10.3969/j.issn.1000-1565.2022.03.002

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This paper first introduces two kinds of novel uncertain data envelopment analysis without explicit inputs: interval-valued data envecopment analysis(DEA)model without explicit input(IDEA-WEI)and fuzzy-valued DEA model without explicit input(FDEA-WEI), then IDEA-WEI model is applied to evaluate the performance of first-class discipline construction, FDEA-WEI model is applied to evaluate the performance of periodical construction of first-class disciplines. The practical application verifies the effectiveness of the uncertain DEA model without explicit inputs.

Analysis of photo-biological safety of indoor LED light sources

YUAN Fangwen, WU Deqi, YANG Qian, ZHOU Xiaojuan, CHU Xiaoxing

2022, 42(3):  241-245.  DOI: 10.3969/j.issn.1000-1565.2022.03.003

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Testing platform for photo-biological safety was built in our laboratory in order to evaluate the potential photo-biological safety risk of indoor LED light sources. Several kinds of indoor LED light sources which were extensively used were tested by this system, mainly including the testing of irradiance and radiance. Furthermore, the work analyzed the experimental results according to the requirement of the standard GB /T 20145—2006: photo-biological safety of lamps and lamp system. At last, the hazard class of the light sources was evaluated and determined. The results have a certain reference significance for the research of testing system and testing method for photo-biological safety of the LED products.

Study on the content of SO₂ in SF₆ by the Fourier transform infrared spectrum

LI Mingyang, MA Xiaoyan, ZHAO Jing, YUAN Xiaoxian, LI Xu, GUAN Li

2022, 42(3):  246-250.  DOI: 10.3969/j.issn.1000-1565.2022.03.004

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The SF₆ gas has been used as insulation and has been applied in some power equipments.The precise determination of SO₂ concentration in SF₆ is helpful to predict the purity of SF₆.Fourier transform infrared(FTIR)spectrometer was used to measure the absorption spectra of SO₂ of different concentrations in SF₆ standard samples, and the relationship between SO₂ concentration and characteristic peak absorption was constructed to obtain the standard working curve.In order to improve the accuracy of the experiment, a multi-refletion gas cell was selected.The results show that the experimental device and method can be applied to the monitoring of SF₆ in power switching equipment in real life.

Flow injection chemiluminescence method for determination of acarbose

ZHAO Mian, WANG Beibei, WANG Bing, LI Xianrui

2022, 42(3):  251-255.  DOI: 10.3969/j.issn.1000-1565.2022.03.005

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A simple and rapid flow-injection chemiluminescence(FI-CL)method was developed for the determination of acarbose in tablets based on the fact that acarbose can remarkably enhance the chemiluminescence of Luminol-H₂O₂ in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence relative intensity had a good linear relationship with acarbose concentration in the range of 3×10^-4~2×10^-2 mg/mL with a correlation coefficient of 0.998 7. The detection limit was 2.3×10^-4 mg/mL and RSD(n=11)is 2.7% for 0.01 mg/mL acarbose. The proposed method has been successfully applied for the determination of acarbose in tablets sample and the average recovery was 101.6%.

Preparation technology of Artemisia argyi formula granules

LIANG Siyi, JI Shengnan, TIAN Luxiao, ZHI Jingyi, LI Lili, HA Jing

2022, 42(3):  256-264.  DOI: 10.3969/j.issn.1000-1565.2022.03.006

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In order to reduce the loss of volatile was oil in the preparation of standard decoction and formula granules, the volatile oil was included with β-cyclodextrin(β-CD), and the inclusion rate of volatile oil was used as an evaluation index to optimize the package of volatile oil and β-CD. Gas chromatography was used to determine the inclusion rate. The correlation among decoction pieces, standard decoction and formula granules was investigated by the content determination of index components and fingerprint. The average inclusion rate of volatile oil obtained by inclusion process was 96.40%, and the preparation method of Artemisia argyi standard decoction and formula granules was established. The average content of eucalyptus olein in the formula granules was 0.738 4 mg/g, and the transfer rate was 33.48%. The average content of borneol was 0.334 1 mg/g, and the transfer rate was 25.41%. The inclusion process and the preparation process of formula granules have high stability and good repeatability.The content of index components of standard decoction and formula granules is consistent, which can provide theoretical reference for industrial production of enterprises.

Synthesis and characterization of Waugh-type compounds containing quaternary ammonium cations

LIU Xuemei, GUO Kexin, LI Jinhuan, LI Huimin, HU Ruohui

2022, 42(3):  265-272.  DOI: 10.3969/j.issn.1000-1565.2022.03.007

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The new Waugh compounds(NH₄)₄［N(CH₃)₄］₂［MnMo₉O₃₂］·9H₂O(1)and(NH₄)₄［N(CH₂CH₃)₄］₂［MnMo₉O₃₂］·7H₂O(2)containing quaternary ammonium cations were obtained by reacting of(NH₄)₆MnMo₉O₃₂ and tetramethyl ammonium chloride or tetraethyl ammonium chloride in aqueous solution, and properties were characterized by elemental analysis, IR and TG. The structure of the compound 1 was determined by single crystal X-ray diffraction. The chemical formula of the compound 1 belongs to monoclinic system with space group C2/c, a=2.379(11)nm,b=1.186(5)nm,c=1.600(7)nm,β=102.308(4)°,V=4.413(3)nm³, Z=4, Dc=2.460 g/cm³, R₁=0.041 8, wR₂=0.117 5(I>2σ), GOF=1.058. Exploring the formation rules of the compounds 1 and 2 revealed that the optimal ratio of the amount of［N(CH₃)₄］Cl or［N(C₂H₅)₄］Cl to(NH₄)₆MnMo₉O₃₂·9H₂O is equal to 3∶1, and that single crystals of title compounds were easily formed. Methylene blue simulated dye wastewater for photocatalytic degradation by compounds 1 and 2 was explored, the decolorization of methylene blue solutions at 90 min and 180 min reached 95.96% and 85.64%, respectively. The photocatalytic results show- DOI:10.3969/j.issn.1000-1565.2022.03.007季铵盐阳离子的Waugh型化合物的合成与表征刘雪梅,郭珂欣,李金环,李慧敏,胡若晖( 西安石油大学 化学化工学院,陕西 西安 710065)摘 要:以(NH₄)₆［MnMo₉O₃₂］为前体原料,采用常规的水溶液法与四甲(乙)氯化铵反应制备了2种含有混合阳离子的Waugh型钼酸盐(NH₄)₄［N(CH₃)₄］₂［MnMo₉O₃₂］·9H₂O(化合物1)和(NH₄)₄［N(CH₂CH₃)₄］₂［MnMo₉O₃₂］·7H₂O(化合物2),用元素分析、红外光谱、热重分析对其进行了表征,并对化合物1进行了单晶结构测定,化合物1属于单斜晶系,C2/c空间群,a=2.379(11)nm,b=1.186(5)nm,c=1.600(7)nm,β=102.308(4)°,V=4.413(3)nm³,Z=4,Dc=2.460 g/cm³,R₁=0.041 8,wR₂=0.117 5(I>2σ),GOF=1.058.对化合物的形成规律探索发现:季铵盐与(NH₄)₆MnMo₉O₃₂的物质的量之比为3∶1,易形成单晶.对化合物1和2光催化降解亚甲基蓝模拟废水进行了探索,光催化90 min和180 min时脱色率分别达到95.96%和85.64%,表明化合物1和2对亚甲基蓝染料废水均具有良好的光催化活性.关键词:Waugh结构;季铵盐;晶体结构;表征;光催化 中图分类号:O611.2 文献标志码:A 文章编号:1000-1565(2022)03-0265-08Synthesis and characterization of Waugh-type compounds containing quaternary ammonium cationsLIU Xuemei, GUO Kexin, LI Jinhuan, LI Huimin, HU Ruohui( College of Chemistry and Chemical Engineering, Xian Shiyou University, Xian 710065, China )Abstract: The new Waugh compounds(NH₄)₄［N(CH₃)₄］₂［MnMo₉O₃₂］·9H₂O(1)and(NH₄)₄［N(CH₂CH₃)₄］₂［MnMo₉O₃₂］·7H₂O(2)containing quaternary ammonium cations were obtained by reacting of(NH₄)₆MnMo₉O₃₂ and tetramethyl ammonium chloride or tetraethyl ammonium chloride in aqueous solution, and properties were characterized by elemental analysis, IR and TG. The structure of the compound 1 was determined by single crystal X-ray diffraction. The chemical formula of the compound 1 belongs to monoclinic system with space group C2/c, a=2.379(11)nm,b=1.186(5)nm,c=1.600(7)nm,β=102.308(4)°,V=4.413(3)nm³, Z=4, Dc=2.460 g/cm³, R₁=0.041 8, wR₂=0.117 5(I>2σ), GOF=1.058. Exploring the formation rules of the compounds 1 and 2 revealed that the optimal ratio of the amount of［N(CH₃)₄］Cl or［N(C₂H₅)₄］Cl to(NH₄)₆MnMo₉O₃₂·9H₂O is equal to 3∶1, and that single crystals of title compounds were easily formed. Methylene blue simulated dye wastewater for photocatalytic degradation by compounds 1 and 2 was explored, the decolorization of methylene blue solutions at 90 min and 180 min reached 95.96% and 85.64%, respectively. The photocatalytic results show- 收稿日期:2021-09-21 基金项目:陕西省自然科学基础研究计划项目(2016JQ2037);国家级大学生创新创业训练计划项目(S202010705018);西安石油大学研究生创新与实践能力培养项目(YCS20211018) 第一作者:刘雪梅(1976—),女,陕西西安人,西安石油大学副教授,博士.主要从事杂多化合物方向的研究.E-mail: lxm2503@163.com第3期刘雪梅等:季铵盐阳离子的Waugh型化合物的合成与表征that both compounds 1 and 2 have good photocatalytic activity against methylene blue simulated dye wastewater.

Vapor-phase reaction during co-pyrolysis of cellulose and lignin

YANG Huamei, JIANG Ju, LIU Wang, ZHANG Bingzhe, XU Panpan

2022, 42(3):  273-280.  DOI: 10.3969/j.issn.1000-1565.2022.03.008

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Volatile reaction is inevitable in the process of biomass pyrolysis, which determines the products formation and distribution from biomass pyrolysis. Co-pyrolysis of cellulose and lignin and theoretical calculation were used to investigate the interaction between cellulose and lignin during biomass pyrolysis, so as to provide an experimental basis for further understanding the pyrolysis behavior of biomass. Results showed: during the co-pyrolysis of cellulose and lignin, the distribution of various products is closely related to the ratio of cellulose and lignin, but the product distribution cannot be predicted by the weighted calculation. Co-pyrolysis of cellulose and lignin in arbitrary ratio inhibited the formation of CO, but promoted the formation of CO₂ and H₂O. Co-pyrolysis of cellulose and lignin promoted the formation of C₁—C₅ hydrocarbons, and inhibited the formation of most oxygen-containing compounds, and promoted the formation of phenol and alkyl phenol when the mixing mass ratio was 5∶5. When the ratio of cellulose and lignin was low, it can promote the formation of aromatic hydrocarbons, but when the ratio of cellulose and lignin was 7∶3, the formation of aromatic hydrocarbons was inhibited.

Establishment and performance evaluation of chemiluminescent immunoassay for serum HER2 protein

WANG Juan, CHENG Hua, HU Chenguang, LIN Si, YU Fei, LIU Tao, LIU Pengpeng

2022, 42(3):  281-287.  DOI: 10.3969/j.issn.1000-1565.2022.03.009

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This study established magnetic particles chemiluminescence method for the detection of serum human epidermal growth factor receptor 2(HER2)protein in the patients with breast cancer, and evaluated its performance and equivalence with market products. The detection range of HER2 protein was from 0 to 300 ng/mL; limit of blank(LoB)was 0.48 ng/mL; average recovery rates of exogenous and endogenous interference was lower than 5%; precision was coefficient of variation(CV)lower than 8%; the stability test showed that the reagent could be stored stably for 48 weeks, and the correlation coefficient with commercial products was r=0.995, the mean bias was -0.2 ng/mL. These results indicated that the detection method of serum HER2 protein was successfully established, and had an excellent- DOI:10.3969/j.issn.1000-1565.2022.03.009血清HER2蛋白化学发光免疫检测方法的建立及评价王娟^1,2,程华³,胡琛光^4,5,林斯^4,5,于飞^{1, 2},刘涛¹,刘朋朋¹(1.河北农业大学 河北野生动物健康研究中心,河北 保定 071000;2.河北农业大学 生命科学学院,河北 保定 071000;3.河北省科学院 生物研究所,河北 石家庄 050081;4.北京华科泰生物技术股份有限公司,北京 101111;5.天津华科泰生物技术有限公司,天津 300405)摘 要:为检测乳腺癌患者血清人表皮生长因子受体2(HER2)蛋白,基于磁微粒化学发光免疫平台建立了血清HER2蛋白检测方法并评价其性能和市场产品等效性.结果显示:本研究建立的血清HER2蛋白检测方法检测范围为0~300 ng/mL;空白限(limit of blank, LoB)为0.48 ng/mL;外源性和内源性干扰平均回收率<5%;精密度变异系数(coefficient of variation,CV)<8%;稳定性实验显示,试剂可稳定储存48周,且与市售产品相关系数r为0.995;平均偏倚为-0.2 ng/mL.提示本研究成功建立了HER2蛋白检测方法,且各项分析性能表现优异,与在售化学发光试剂等效性良好,可为临床应用提供科学的数据参考.关键词:乳腺癌;HER2;化学发光法;性能评价;等效性中图分类号:R737.9 文献标志码:A 文章编号:1000-1565(2022)03-0281-07Establishment and performance evaluation of chemiluminescent immunoassay for serum HER2 proteinWANG Juan^1,2, CHENG Hua³, HU Chenguang^4,5, LIN Si^4,5, YU Fei^1,2, LIU Tao¹, LIU Pengpeng¹(1. Hebei Wildlife Health Center, Hebei Agricultural University, Baoding 071000, China;2. College of Life Science, Hebei Agricultural University, Baoding 071000, China;3. Institute of Biology, Hebei Academy of Sciences, Shijiazhuang 050081, China;4. Beijing Savant Biotechnology Co. Ltd., Beijing 101111, China;5. Tianjin Savant Biotechnology Co. Ltd., Tianjin 300405,China)Abstract: This study established magnetic particles chemiluminescence method for the detection of serum human epidermal growth factor receptor 2(HER2)protein in the patients with breast cancer, and evaluated its performance and equivalence with market products. The detection range of HER2 protein was from 0 to 300 ng/mL; limit of blank(LoB)was 0.48 ng/mL; average recovery rates of exogenous and endogenous interference was lower than 5%; precision was coefficient of variation(CV)lower than 8%; the stability test showed that the reagent could be stored stably for 48 weeks, and the correlation coefficient with commercial products was r=0.995, the mean bias was -0.2 ng/mL. These results indicated that the detection method of serum HER2 protein was successfully established, and had an excellent- 收稿日期:2022-01-12 基金项目:国家重点研发计划项目(2020YFC084310);河北省科学院科技攻关项目(21313) 第一作者:王娟(1989—),女,河北廊坊人,河北农业大学副教授,主要从事抗肿瘤药物筛选和相关机制研究.E-mail: wangjuan@hebau.edu.cn 通信作者:刘朋朋(1985—),男,河北赵县人,河北农业大学研究员,博士,主要从事体外诊断技术研究.E-mail: liupeng@cwbz.ac.cn第3期王娟等:血清HER2蛋白化学发光免疫检测方法的建立及评价performance compared with HER2 protein chemiluminescence reagents sold on the market.

Effects of endoplasmic reticulum stress on adipose tissue in mice

YAO Songhui, CHEN Hui, YANG Yang, WAN Yue, CHU Yuan, SUN Lei, YU Miao

2022, 42(3):  288-294.  DOI: 10.3969/j.issn.1000-1565.2022.03.010

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In order to explore the effect of endoplasmic reticulum stress on white adipose tissue in mice, endoplasmic reticulum stress was induced in mice by intraperitoneal injection of tunicamycin.This constructed an endoplasmic reticulum stress mouse model. Then, white fat from the epididymis of mice was obtained. RT-PCR and Western blot were used to detect the mRNA level and protein expression of endoplasmic reticulum stress-related factors in white adipose tissue. The changes of white adipose tissue cells were observed by fluorescence microscope, the content of triglycerides, free fatty acids and the secretion of adipocytokines and inflammatory factors were measured. The results showed that treatment with 1.0 mg/kg tunicamycin for 72 h was the best in inducing endoplasmic reticulum stress in mice. Endoplasmic reticulum stress led to a decrease in the proportion of white adipose tissue, a decrease in fat cell diameter, an increase in lipolysis, a decrease in the secretion of adipocytokines, and an increase in inflammation.- DOI:10.3969/j.issn.1000-1565.2022.03.010内质网应激对小鼠脂肪组织的影响么松慧^1,2,陈慧²,杨洋^2,3,万悦^2,4,初源²,孙磊¹,于淼²(1.河北大学 生命科学学院,河北 保定 071002;2.军事医学科学院 生命组学研究所,北京 100850;3.天津大学 化工学院,天津 300072;4.安徽医科大学 基础医学院,安徽 合肥 230032)摘 要:为了探究内质网应激对小鼠白色脂肪组织的影响,通过腹腔注射衣霉素(tunicamycin, TM)诱导小鼠体内内质网应激,构建内质网应激小鼠模型,然后获取小鼠附睾处白色脂肪组织,并采用RT-PCR和Western blot检测白色脂肪组织中内质网应激相关因子的mRNA水平及蛋白表达情况,通过荧光显微镜观察白色脂肪组织细胞的变化,测定甘油三酯、游离脂肪酸含量及脂肪细胞因子和炎症因子分泌情况.结果表明:1.0 mg/kg TM处理72 h诱导小鼠内质网应激效果最佳;内质网应激可以导致白色脂肪组织比例减少,脂肪细胞直径减小,脂解反应增多,脂肪细胞因子分泌减少及炎症反应增多等.该研究结果说明内质网应激可以诱导小鼠脂肪组织中发生脂解,进而介导脂代谢紊乱.关键词:内质网应激;衣霉素;白色脂肪组织;脂代谢中图分类号:Q591.5 文献标志码:A 文章编号:1000-1565(2022)03-0288-07Effects of endoplasmic reticulum stress on adipose tissue in miceYAO Songhui^1,2, CHEN Hui², YANG Yang^2,3, WAN Yue^2,4, CHU Yuan², SUN Lei¹, YU Miao²(1. School of Life Sciences, Hebei University, Baoding 071002, China; 2. Institute of Lifeomics, Academy of Military Medical Sciences, Beijing 100850, China; 3. School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; 4. School of Basic Medicine, Anhui Medical University, Hefei 230032, China)Abstract: In order to explore the effect of endoplasmic reticulum stress on white adipose tissue in mice, endoplasmic reticulum stress was induced in mice by intraperitoneal injection of tunicamycin.This constructed an endoplasmic reticulum stress mouse model. Then, white fat from the epididymis of mice was obtained. RT-PCR and Western blot were used to detect the mRNA level and protein expression of endoplasmic reticulum stress-related factors in white adipose tissue. The changes of white adipose tissue cells were observed by fluorescence microscope, the content of triglycerides, free fatty acids and the secretion of adipocytokines and inflammatory factors were measured. The results showed that treatment with 1.0 mg/kg tunicamycin for 72 h was the best in inducing endoplasmic reticulum stress in mice. Endoplasmic reticulum stress led to a decrease in the proportion of white adipose tissue, a decrease in fat cell diameter, an increase in lipolysis, a decrease in the secretion of adipocytokines, and an increase in inflammation.- 收稿日期:2021-12-02 基金项目:国家自然科学基金面上项目(82070596) 第一作者:么松慧(1997—),女,河北唐山人,河北大学在读硕士研究生,主要从事肝脏病理生理研究.E-mail:15703277906@163.com 通信作者:孙磊(1971—),女,河北唐山人,河北大学教授,博士,主要从事环境微生物研究.E-mail:sunlei1018@126.com于淼(1978—),女,浙江湖州人,军事医学科学院副研究员,博士,主要从事肝脏病理生理研究.E-mail:13718674282@163.com第3期么松慧等:内质网应激对小鼠脂肪组织的影响The results of this study indicated that endoplasmic reticulum stress induced lipolysis in mouse adipose tissue, leading to lipid metabolism disorder.

Fecal microbiota transplantation inhibited Klebsiella pneumoniae caused liver abscess infection in mice

WANG Ziyiru, ZHU Dongqing, FU Jinwen,CHENG Baoqian, LING Zongxin, CHEN Nan

2022, 42(3):  295-302.  DOI: 10.3969/j.issn.1000-1565.2022.03.011

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This work aims to explore the possibility of fecal microbiota transplantation(FMT)to inhibit hypervirulent Klebsiella pneumoniae caused liver abscess(KLA)infection in mice and its related mechanism. The mice were randomly divided into three groups: Healthy, Kp and FMT. Kp group mice were orally inoculated with 10⁶ CFU of K.pneumoniae isolate; FMT group mice were given FMT(300 mg/mL, 0.3 mL)orally 24 h after K.pneumoniae isolate oral inoculation. Results showed that FMT effectively inhibited the KLA infection in mice at 48 h after K.pneumoniae isolate oral inoculation. The high-throughput sequencing analysis showed that FMT altered the intestinal microbiota composition which has been changed- DOI:10.3969/j.issn.1000-1565.2022.03.011粪菌移植对肺炎克雷伯菌所致小鼠肝脓肿感染的抑制作用王子逸儒¹,朱冬青¹,付金文¹,程宝乾²,凌宗欣³,陈楠¹(1. 河北大学 基础医学院,河北 保定 071000;2. 河北医科大学 临床学院, 河北 石家庄 050031;3.浙江大学 医学院,附属第一医院,传染病诊治国家重点实验室, 浙江 杭州 310003)摘 要:探讨粪菌移植(FMT)对高致病性肺炎克雷伯菌(Klebsiella pneumoniae)所致小鼠肝脓肿(KLA)感染的抑制作用及相关机制. 小鼠随机分为对照(Healthy)组、肺炎克雷伯菌(Kp)组和干预(FMT)组. Kp组小鼠经口灌胃10⁶ CFU K1血清型肺炎克雷伯菌;FMT组在给菌24 h后经口灌胃给予正常小鼠粪菌悬液(300 mg/mL,0.3 mL). 结果表明,与Kp组相比,FMT处理明显降低了小鼠KLA感染率;16S rRNA高通量测序分析表明,FMT显著改变了小鼠肠道菌群组成,包括增加了Alloprevotella、norank_f_Muribaculaceae等益生菌的丰度;同时FMT处理明显降低了小鼠血清TNF-α和胃肠内防御素分子浓度,同时增加了肠黏蛋白2(MUC2)的表达. 因此,肠道菌群和免疫防御因子可能一起参与了FMT对高致病性肺炎克雷伯菌所致小鼠KLA感染的抑制作用. 关键词:粪菌移植;肺炎克雷伯菌;肝脓肿;肠道菌群;黏蛋白2中图分类号:R378 文献标志码:A 文章编号:1000-1565(2022)03-0295-08Fecal microbiota transplantation inhibited Klebsiella pneumoniae caused liver abscess infection in miceWANG Ziyiru¹, ZHU Dongqing¹, FU Jinwen¹,CHENG Baoqian², LING Zongxin³, CHEN Nan¹(1.School of Basic Medical Sciences, Hebei University, Baoding 071000, China; 2.College of Clinical Medicine, Hebei Medical University, Shijiazhuang 050031, China; 3. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China)Abstract: This work aims to explore the possibility of fecal microbiota transplantation(FMT)to inhibit hypervirulent Klebsiella pneumoniae caused liver abscess(KLA)infection in mice and its related mechanism. The mice were randomly divided into three groups: Healthy, Kp and FMT. Kp group mice were orally inoculated with 10⁶ CFU of K.pneumoniae isolate; FMT group mice were given FMT(300 mg/mL, 0.3 mL)orally 24 h after K.pneumoniae isolate oral inoculation. Results showed that FMT effectively inhibited the KLA infection in mice at 48 h after K.pneumoniae isolate oral inoculation. The high-throughput sequencing analysis showed that FMT altered the intestinal microbiota composition which has been changed- 收稿日期:2021-10-22 基金项目:河北省高等学校科学技术研究项目青年项目(QN2020111);河北省博士后科研项目(B2017003032);河北大学优秀青年科研创新团队(科技类)项目(2020-8);河北大学医学学科培育项目(2020B10);河北大学大学生创新创业项目(2021292) 第一作者:王子逸儒(1997—),女,河北易县人,河北大学在读硕士研究生,主要从事肠道微生态与疾病的相关研究.E-mail:799241689@qq.com 通信作者:陈楠(1981—),女,河北安国人,河北大学副教授,主要从事肠道微生态与疾病的相关研究.E-mail:cnadyheu@126.com第3期王子逸儒等:粪菌移植对肺炎克雷伯菌所致小鼠肝脓肿感染的抑制作用by K.pneumoniae oral inoculation, including the up-regulation of the abundance of the probiotics Alloprevotella, norank_f_Muribaculaceae and so on. FMT also reduced the levels of defensins in intestine and stomach, inflammatory cytokine TNF-α in serum, and increased the concentration of mucin 2 in intestine. Therefore, the intestinal microbiota and immune defense factors may be involved in the inhibition of FMT against liver abscess infection caused by hypervirulent K.pneumoniae in mice.

A new record species of Dusmetia Mercet from China (Hymenoptera: Encyrtidae)

MENG Jisen, ZHANG Chen, XUE Hao, ZU Guohao

2022, 42(3):  303-305.  DOI: 10.3969/j.issn.1000-1565.2022.03.012

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A species of Encyrtidae, Dusmetia pulex(Ruschka, 1923), is firstly recorded from Xilinhaote City, Inner Mongolia, China. The photos of adult are provided, and a key to Chinese known species of Dusmetia is also given.

SAR image denoising based on generative adversarial networks

LIU Shuaiqi, LEI Yu, PANG Jiao, ZHAO Shuhuan, SU Yonggang, SUN Chenyang

2022, 42(3):  306-313.  DOI: 10.3969/j.issn.1000-1565.2022.03.013

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Synthetic aperture radar(SAR)is a kind of active earth-observation system, which can produce high-resolution image all day and it has been widely used in agriculture and military. However, SAR image is affected by speckle due to the coherent imaging mechanism. In this paper, we propose a SAR image denoising algorithm based on generative adversarial network.We constructed the generated network by the structure of deep convolutional neural network(DCNN)with residual structure. The new generated network can accelerate the training process and improve the denoising performance. We also define a loss function by combining PSNR and SSIM, which makes the denoising result of SAR noisy image more in line with the visual perception requirements of human eyes. The experiment results show that the proposed algorithm can suppress speckle in SAR images and get good denoising result.

Switching pseudo-predictive stabilization controller for input delay systems

LIANG Jiahao, TANG Yujun, WANG Xia

2022, 42(3):  314-320.  DOI: 10.3969/j.issn.1000-1565.2022.03.014

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A switching pseudo-predictor is designed for a class of systems with input delays to achieve accurate state prediction while avoiding the inclusion of input integral terms. It provides a basis and rationale for applying controller design methods of non-delayed systems, which can simplify the stability and dynamic performance analysis of closed-loop systems. The original system is divided into two subsystems, the open-loop one and the closed-loop one, bythe moment when the control quantity actually affects the system state, and a pseudo-predictor with switching structure is constructed to predict the system state accordingly.A stabilizing controller designed using the predicted state to design for the input delayed linear system and the asymptotic stability of the closed-loop system is demonstrated.

Position recognition of the vehicle ahead and safety distance monitoring based on monocular vision

ZHANG Xin, WEI Zhiheng, WANG Bing

2022, 42(3):  321-326.  DOI: 10.3969/j.issn.1000-1565.2022.03.015

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A solution based on monocular vision is proposed to recognize the position of the vehicle ahead and to monitor the safe distance in automatic driving. Firstly, whether the vehicle ahead is a monitoring vehicle can be judged by whether the contour of the vehicle ahead intersects with the roadway and the dividing line of the roadway. Secondly, according to the corresponding relationship between pixel coordinate system and spatial coordinate system in the image, the transformation model is built to calculate the real distance from the monitored vehicle. Finally, the speed of this vehicle is calculated according to the position change of the crossable delimitation line of the same direction roadway in the continuous image frames, and the safety distance between this vehicle and the monitored vehicle is determined according to the traffic laws and regulations. Experimental results show that this solution can effectively recognize the position of the vehicle ahead, monitor the safety distance and reduce the system cost.

A real-time method for colorectal adenoma detection based on an improved YOLOv5s network

LIU Shuang, TIAN Zhaoxing, LI Haoran, CHANG Ying, WU Sibei, XUE Linyan

2022, 42(3):  327-336.  DOI: 10.3969/j.issn.1000-1565.2022.03.016

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There are two major issues in the existing deep learning-based algorithms for colorectal polyp detection which are leading to the failure of real-time detection: one is the complicate calculation and the other is poor detection precision. In this paper, we proposed a real-time method for colorectal adenoma detection based on a single-stage target detection network YOLOv5s, in which the channel attention mechanism was added to the backbone and the loss function of BCEWithLogitsLoss was adopted instead of the original one of BCELogits. We collected 2074 endoscopic adenoma images and 20 adenoma videos with 19700 frames, and then assigned them as training dataset and testing dataset according to the ratio of 3∶1. - DOI:10.3969/j.issn.1000-1565.2022.03.016一种基于改进YOLOv5s网络的结直肠腺瘤实时检测方法刘爽¹,田兆星¹,李浩然¹,常颖²,吴思蓓¹,薛林雁¹(1.河北大学 质量技术监督学院,计量仪器与系统国家地方联合工程研究中心,河北 保定 071002;2.河北大学附属医院 消化内科,河北 保定 071000)摘 要:为解决现有的基于深度学习的结直肠息肉检测算法计算复杂或检测精度较低,不能在速度和精度方面同时满足实时检测的问题,提出了一种基于单阶段目标检测网络YOLO(you only look once)v5s的结直肠腺瘤实时检测方法.在YOLOv5s的主干网中融入通道注意力机制,并以BCEWithLogitsLoss代替其原有的交叉熵损失函数BCELogits,以此提升网络性能.选取2 074张腺瘤图片和包含19 700帧的20段腺瘤视频,按照3∶1的比例构建结直肠腺瘤训练集和测试集.测试结果表明,结直肠腺瘤检测的平均精度为93.6%,检测速度为93帧/s,验证了该系统可以在肠镜的退镜过程中实时检测腺瘤性息肉,且具有较好的检测性能.关键词:计算机辅助诊断;腺瘤;卷积神经网络;YOLOv5s;实时检测中图分类号:TP391.7 文献标志码:A 文章编号:1000-1565(2022)03-0327-10A real-time method for colorectal adenoma detection based on an improved YOLOv5s networkLIU Shuang¹, TIAN Zhaoxing¹, LI Haoran¹, CHANG Ying², WU Sibei¹, XUE Linyan¹(1. National & Local Joint Engineering Research Center of Metrology Instrument and System, College of Quality and Technical Supervision, Hebei University, Baoding 071002, China; 2. Department of Gastroenterology, Affiliated Hospital of Hebei University, Baoding 071000, China)Abstract: There are two major issues in the existing deep learning-based algorithms for colorectal polyp detection which are leading to the failure of real-time detection: one is the complicate calculation and the other is poor detection precision. In this paper, we proposed a real-time method for colorectal adenoma detection based on a single-stage target detection network YOLOv5s, in which the channel attention mechanism was added to the backbone and the loss function of BCEWithLogitsLoss was adopted instead of the original one of BCELogits. We collected 2074 endoscopic adenoma images and 20 adenoma videos with 19700 frames, and then assigned them as training dataset and testing dataset according to the ratio of 3∶1. - 收稿日期:2021-10-14 基金项目:河北省自然科学基金资助项目(H2019201378);河北大学自然科学多学科交叉研究计划资助项目(DXK201914);河北大学校长科研基金资助项目(XZJJ201914;XZJJ201917;XZJJ201918);大中学生科技创新能力培育专项项目(22E50041D) 第一作者:刘爽(1981—),女,河北保定人,河北大学副教授,博士,主要从事生物医学图像处理方向研究.E-mail:lianlianfushi@126.com 通信作者:薛林雁(1981—),女,河北广平人,河北大学副教授,博士,主要从事生物医学图像处理、计算机视觉方向研究.E-mail:lineysnow@163.com第3期刘爽等:一种基于改进YOLOv5s网络的结直肠腺瘤实时检测方法The experimental results show that the improved network achieved a mean average precision of 93.6% and a detection speed of 93FPS, which implies that the system can detect adenomatous polyps in real time during the retraction of colonoscopy, and outperforms the original state-of-the-art method.