Table of Content

25 July 2022, Volume 42 Issue 4

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Parameter estimation of time censored exponential life data under ranked set sampling

DONG Xiaofang, GAO Yang, ZHANG Liangyong

2022, 42(4):  337-342.  DOI: 10.3969/j.issn.1000-1565.2022.04.001

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In order to solve the problem of parameter estimation for time censored exponential life data, the maximum likelihood estimation using ranked set sampling method is studied. For the problem that likelihood equation’s root under ranked set sampling is not found, some terms of likelihood equation are replaced with their expectations, and the modified maximum likelihood estimation is given. The comparison results of estimation efficiencies show that the proposed estimator using ranked set sampling method is better than maximum likelihood estimator using simple random sampling method.

Infinite dimensional homogenous Rota-Baxter 3-Lie algebras(Ⅱ)

BAI Ruipu, LIU Shan

2022, 42(4):  343-349.  DOI: 10.3969/j.issn.1000-1565.2022.04.002

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13 classes of homogeneous Rota-Baxter 3-Lie algebras A_k are constructed by using the homogeneous Rota-Baxter operators R_k, 1≤k≤13, with weight 1 and satisfying g(0)+g(1)+1=0 on infinite dimensional simple 3-Lie algebra A_ω. It is proved that there are 5 classes of non-isomorphic homogeneous Rota-Baxter 3-Lie algebras D_i,1≤i≤5, in the homogeneous Rota-Baxter 3-Lie Algebra A_k,1≤k≤13, and the structure of each class of 3-Lie algebras D_i, 1≤i≤5, is studied.

A class of linear codes from Weil sums

LU Hong, YANG Shudi, ZHANG Tonghui

2022, 42(4):  350-357.  DOI: 10.3969/j.issn.1000-1565.2022.04.003

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It is of vital significance to explore the weight distributions of linear codes with a few weights in coding theory. By selecting an appropriate defining set, a new class of two-weight or three-weight linear codes is constructed. Then, their weight distributions are determined by employing exponential sums over finite fields. Furthermore, some examples to verify the correctness of our results were gived and some of them are optimal codes with respect to the Griesmer bound.

Stress and deformation analysis of bamboo net in soft soil foundation reinforcement

WANG Guixiang, YAN Shengkang, LE Shaolin, WANG Haochen, YU Zenghui

2022, 42(4):  358-363.  DOI: 10.3969/j.issn.1000-1565.2022.04.004

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In order to study the effect, stress and deformation of bamboo net in surface treatment of soft soil foundation, the experimental material parameters were determined through experiments, and the indoor model load test of bamboo net reinforced soft foundation with hydraulic jack was designed to study the deformation and stress characteristics of bamboo net under load. The test results show that the failure mode of bamboo single pole simply supported beam under concentrated load is longitudinal cracking or bending, the bending ratio limit is 6.6—107.2 MPa, the ultimate strength is 71.9—117.8 MPa, and the elastic modulus is 11.59—19.57 GPa. The bearing capacity of bamboo net with a spacing of 15 cm×15 cm is 3.7—5.9 times that of a single bamboo rod. With the increase of the thickness of the overlying soil layer, the foundation pressure increases obviously, and when the thickness width ratio is 0—0.69, the foundation pressure over that of soft soil foudation with a corresponding settlement of 40 mm is 2.66—6.96. The strain and stress of bamboo net decrease due to the diffusion of sandy soil layer.

Physical properties of La_0.67Sr_0.33MnO₃/PbZr_0.4Ti_0.6O₃/La_0.67Sr_0.33 MnO₃ epitaxial ferroelectric capacitor

XUE Yinggang,MENG Hao,WANG Zhaowen,ZHAO Wenze,LIU Baoting,DAI Xiuhong

2022, 42(4):  364-369.  DOI: 10.3969/j.issn.1000-1565.2022.04.005

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La_0.67Sr_0.33MnO₃(LSMO)/PbZr_0.4Ti_0.6O₃(PZT)/LSMO heterostructure was prepared on SrTiO₃(STO)substrate, in which LSMO films were prepared by pulsed laser deposition and PZT film was prepared by Sol-Gel method. The LSMO/PZT/LSMO heterostructure was characterized in terms of its structural and physical properties. X-ray diffraction(XRD)analysis established that both LSMO and PZT were grown epitaxially on STO substrate. The LSMO/PZT/LSMO capacitor possessed very good ferroelectric hysteresis property and its remnent polarization and coercive voltage of the LSMO/PZT/LSMO capacitor, measured at 5 V, were 26.9 μC/cm² and 2.5 V, respectively. The LSMO/PZT/LSMO capacitor exhibited very good fatigue resistant property. The leakage current density of the capacitor measured at 5 V was 7.4×10^-6 A/cm², and it met the ohmic conduction mechanism below 2.9 V, and space charge limited current(SCLC)conduction from 2.9 V to 5 V.- DOI:10.3969/j.issn.1000-1565.2022.04.005La_0.67Sr_0.33MnO₃/PbZr_0.4Ti_0.6O₃/La_0.67Sr_0.33MnO₃外延铁电电容器物理性能薛迎港¹,孟浩¹,王照文¹,赵文泽²,刘保亭¹,代秀红¹(1.河北大学 物理科学与技术学院,河北 保定 071002;2.河北大学 电子信息工程学院,河北 保定 071002)摘 要:采用脉冲激光沉积法制备La_0.67Sr_0.33MnO₃(LSMO)薄膜,溶胶-凝胶法制备PbZr_0.4Ti_0.6O₃(PZT)薄膜,在SrTiO₃(STO)基片上制备了LSMO/PZT/LSMO异质结电容器.X线衍射(XRD)研究表明LSMO和PZT薄膜在STO基片上实现了外延生长.LSMO/PZT/LSMO电容器具有良好的铁电性能,在5 V测试电压下,剩余极化强度为26.9 μC/cm²,矫顽电压为2.5 V,LSMO/PZT/LSMO电容器显示出了良好的抗疲劳性能.5 V电压下电容器的漏电流密度为7.4×10^-6 A/cm²,在0~2.9 V为欧姆导电机制,2.9~5 V满足空间电荷限制电流(SCLC)导电机制. 关键词:铁电电容器;锆钛酸铅;镧锶锰氧;脉冲激光沉积法;溶胶-凝胶法;漏电机理中图分类号:O469 文献标志码:A 文章编号:1000-1565(2022)04-0364-06Physical properties of La_0.67Sr_0.33MnO₃/PbZr_0.4Ti_0.6O₃/La_0.67Sr_0.33MnO₃ epitaxial ferroelectric capacitorXUE Yinggang¹,MENG Hao¹,WANG Zhaowen¹,ZHAO Wenze²,LIU Baoting¹,DAI Xiuhong¹(1. College of Physics Science and Technology, Hebei University, Baoding 071002, China;2. College of Electronic and Information Engineering, Hebei University, Baoding 071002, China)Abstract: La_0.67Sr_0.33MnO₃(LSMO)/PbZr_0.4Ti_0.6O₃(PZT)/LSMO heterostructure was prepared on SrTiO₃(STO)substrate, in which LSMO films were prepared by pulsed laser deposition and PZT film was prepared by Sol-Gel method. The LSMO/PZT/LSMO heterostructure was characterized in terms of its structural and physical properties. X-ray diffraction(XRD)analysis established that both LSMO and PZT were grown epitaxially on STO substrate. The LSMO/PZT/LSMO capacitor possessed very good ferroelectric hysteresis property and its remnent polarization and coercive voltage of the LSMO/PZT/LSMO capacitor, measured at 5 V, were 26.9 μC/cm² and 2.5 V, respectively. The LSMO/PZT/LSMO capacitor exhibited very good fatigue resistant property. The leakage current density of the capacitor measured at 5 V was 7.4×10^-6 A/cm², and it met the ohmic conduction mechanism below 2.9 V, and space charge limited current(SCLC)conduction from 2.9 V to 5 V.- 收稿日期:2021-08-31 基金项目:国家自然科学基金资助项目(11374086);河北省自然科学基金资助项目(E2014201188;A2018201168) 第一作者:薛迎港(1997—),男,河北石家庄人,河北大学在读硕士研究生,主要从事铁电薄膜及器件方向研究.E-mail:yinggang_xue@hotmail.com 通信作者:代秀红(1978—)女,河北定州人,河北大学副教授,主要从事钙钛矿结构超导、铁电、介电等氧化物薄膜及器件方向研究.E-mail:daixiuhong@hbu.edu.cn第4期薛迎港等:La_0.67Sr_0.33MnO₃/PbZr_0.4Ti_0.6O₃/La_0.67Sr_0.33MnO₃外延铁电电容器物理性能

Atomic layer deposition process of SnO₂ and TiO₂ films on perovskite materials

LUO Hao, ZHANG Huiyu, LIU Hongyan, LIU Haixu, LU Wanbing

2022, 42(4):  370-375.  DOI: 10.3969/j.issn.1000-1565.2022.04.006

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The diffusion reaction between the metal electrode and the halogen is an important factor of perovskite solar cell(PSC)degradation. Using atomic layer deposition(ALD)technology to deposit a dense buffer layer with good electrical performance between metal electrode and perovskite active layer is one of the preferred solutions to solve the above problems. This work studies the process compatibility of SnO₂ and TiO₂ films deposited on organohalide perovskite materials using ALD technology. The effects of tetra(dimethylamino)titanium(TDMATi)and tetra(dimethylamino)tin(TDMASn)pulses and water pulses at different temperatures on the structure of perovskite films are investigated by X-ray diffraction and ultraviolet-visible absorption spectroscopy, and the influence of the pulse times of different precursor sources and purging time on the film deposition mode are analyzed, and the optimized ALD processes of SnO₂- DOI:10.3969/j.issn.1000-1565.2022.04.006SnO₂和TiO₂薄膜在钙钛矿材料上的原子层沉积工艺罗浩,张慧玉,刘红燕,刘海旭,路万兵(河北大学 物理科学与技术学院,河北省光电信息材料重点实验室,河北 保定 071002)摘 要:金属电极与卤素之间的扩散反应是造成钙钛矿太阳电池(PSC)衰退的重要因素,而利用原子层沉积(ALD)技术在金属电极和钙钛矿活性层之间沉积致密且电学性能良好的缓冲层,是解决上述问题的优选方案之一.本文主要研究了利用ALD技术在金属有机卤化物钙钛矿材料上沉积SnO₂和TiO₂薄膜的工艺兼容性问题.通过X线衍射和紫外-可见吸收光谱等技术,表征了分别作为钛源和锡源的四(二甲氨基)钛(TDMATi)和四(二甲氨基)锡(TDMASn)脉冲以及不同温度下水脉冲对钙钛矿薄膜结构的影响,分析了不同前驱体源的脉冲及吹扫时间对薄膜沉积模式的影响,获得了与钙钛矿材料相兼容的SnO₂和TiO₂薄膜的优化ALD工艺.将优化后的SnO₂和TiO₂薄膜ALD工艺应用于倒置PSC制备,对电池的J-V曲线和大气环境下的稳定性测试结果表明,基于ALD技术沉积的SnO₂和TiO₂缓冲层的引入,使得PSC的稳定性明显改善,而且其功率转换效率也有所提高.关键词:钙钛矿;原子层沉积;工艺兼容性;稳定性中图分类号:O469 文献标志码:A 文章编号:1000-1565(2022)04-0370-06Atomic layer deposition process of SnO₂ and TiO₂ films on perovskite materialsLUO Hao, ZHANG Huiyu, LIU Hongyan, LIU Haixu, LU Wanbing(Hebei Key Laboratory of Optic-Electronic Information and Materials, College of Physics Science and Technology, Hebei University, Baoding 071002, China)Abstract: The diffusion reaction between the metal electrode and the halogen is an important factor of perovskite solar cell(PSC)degradation. Using atomic layer deposition(ALD)technology to deposit a dense buffer layer with good electrical performance between metal electrode and perovskite active layer is one of the preferred solutions to solve the above problems. This work studies the process compatibility of SnO₂ and TiO₂ films deposited on organohalide perovskite materials using ALD technology. The effects of tetra(dimethylamino)titanium(TDMATi)and tetra(dimethylamino)tin(TDMASn)pulses and water pulses at different temperatures on the structure of perovskite films are investigated by X-ray diffraction and ultraviolet-visible absorption spectroscopy, and the influence of the pulse times of different precursor sources and purging time on the film deposition mode are analyzed, and the optimized ALD processes of SnO₂- 收稿日期:2021-01-07 基金项目:河北省自然科学基金资助项目(E2021201014;F2017201101);中央引导地方科技发展资金项目(206Z1102G) 第一作者:罗浩(1992—),男,吉林松原人,河北大学在读硕士研究生,主要从事新能源光电功能材料研究. E-mail:1683209529@qq.com 通信作者:路万兵(1980—),男,河北保定人,河北大学副教授,博士,主要从事新能源光电功能材料研究.E-mail:wanbinglu@hbu.edu.cn第4期罗浩等:SnO₂和TiO₂薄膜在钙钛矿材料上的原子层沉积工艺and TiO₂ films compatible with perovskite materials are obtained. The inverted PSCs are fabricated by using the optimized SnO₂ and TiO₂ film ALD process, and the J-V curves and stability tests in atmospheric environment for the PSCs show that the introduction of SnO₂ and TiO₂ buffer layers deposited by ALD technology significantly improves the stability of PSCs, and their power conversion efficiency has also been improved.

Recovery of potato strach during separation and characterization process using field-flow fractionation

ZHANG Wenhui, SONG Tiange, SUN Yushan, SHEN Shigang, DOU Haiyang

2022, 42(4):  376-383.  DOI: 10.3969/j.issn.1000-1565.2022.04.007

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Potato starch was separated and characterized by field-flow fractionation. The interactions between the sample and the surface of ultrafiltration membrane in AF4 were investigated. The effects of ionic strength of carrier liquid, injection mass, cross-flow rate, and focusing time on the sample recovery were evaluated. The repeatability of the results was examined. The highest recovery of potato starch(97.30±0.02)% was obtained with a carrier liquid containing 5 mmol/L NaNO₃ and 3 mmol/L NaN₃, pH 7.00; injection mass, 15 μg; cross-flow rate, 1.0 mL/min; focusing time, 2.5 min. The method developed in this study showed good repeatability in terms of radius of gyration and molar mass with their relative standard deviations of 1.18% and 5.67%, respectively.

Optimization of preparation of peanut shell biochar by response surface methodology and its adsorption of Cr(Ⅵ)in water

DENG Tiantian,LI Hansheng, MA Pei, XU Mengxing, YE Zixin, ZHAI Liping

2022, 42(4):  384-394.  DOI: 10.3969/j.issn.1000-1565.2022.04.008

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In order to explore the removal effect of biomass charcoal on heavy metal ions, a preparation conditions of the optimal biomass carbon were obtained by response surface optimization method well using peanut shell as raw material and ZnCl₂ as modifier. In addition, the effects of different single factors on the adsorption of Cr(Ⅵ)in water by peanut shell biomass carbon were studied. The results showed that the optimum preparation conditions of peanut shell biochar were as follows: ZnCl₂ modifier concentration as 4 mol/L, carbon burning temperature as 500 ℃, carbon burning time as 3 h. The single factor experiment showed that the optimum reaction condition was pH=2 and dosage=50 mg. At this time, the adsorption rate can reach to 93.72%, and the higher the temperature resulted the more conducive to adsorption. The isothermal adsorption experiment conforms to the Langmuir model, which showed that the adsorption process was dominated by monolayer adsorption, which belongs to endothermic reaction. The kinetics accorded with the quasi second order equation, indicating that the adsorption process was mainly chemisorption.

Determination of trace bromophenols in water by precolumn derivatization-SPE-GC/MS

GE Jiayu, LIANG Haiyan, LIU Dong, LI Chunling, ZHANG Fengquan, ZHAO Qian

2022, 42(4):  395-402.  DOI: 10.3969/j.issn.1000-1565.2022.04.009

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In this work, bromophenols(BPs)in water samples were enriched by solid phase extraction, with acetic anhydride as derivative reagent and gas chromatography-mass spectrometer as detection instrument and an analysis method suitable for trace BPs in water was established. The method was evaluated by the standard recovery experiment of municipal water sample. After derivatization, BPs showed good chromatographic response signal. The detection limits were 0.8—17.7 ng/mL. The addition recoveries of blank water and municipal water ranged from 93.2% to 114.5%, 68.3% to 110.1%, and only the addition recoveries of 2,4,6-tribromophenol(2,4,6-TBP)was low, ranged from 68.3% to 73.0%. The relative standard deviations were less than 20%. This method was proved to have good sensitivity, recovery and repeatability, and can be applied for the determination of BPs in actual water samples.

Effects of oleanolic acid on the expression of key genes of insulin/IGF1 pathway in leydig cells of aging rats

LI Shaohua, NIU Shuang, DONG Ziyi,SONG Yicheng, NIU Siyun

2022, 42(4):  403-410.  DOI: 10.3969/j.issn.1000-1565.2022.04.010

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To investigate the effect of oleanolic acid on leydig cells of aging rats, the MTT method was used to determine the optimal drug concentration of oleanolic acid. The aging module of rat leydig cell was built according to the free radical oxidative damage method. Gene and protein expression of the key gene in the insulin/insulin-like growth factor 1(IGF1)signaling pathway was detected by RT-qPCR, immunofluorescence technique(IF), and Western blot. After treated with INSR inhibitor, cell apoptosis was performed by flow cytometry. The results showed that INSR, IRS1, IRS2 and IGF1 mRNA transcription level and protein expression level were significantly downregulated(P<0.05); IGFBP3 mRNA transcription level was significantly upregulated(P<0.05), otherwise, the expression could not be detected by IF and Western blot. Oleanolic acid reversed the above phenomenon, compared to aging group(P<0.05).- DOI:10.3969/j.issn.1000-1565.2022.04.010齐墩果酸对衰老大鼠睾丸间质细胞胰岛素/IGF1通路关键基因表达的影响李少华^1,2,牛双²,董子怡²,宋邑诚²,牛嗣云²(1. 河北大学附属医院 新生儿科,河北 保定 071000;2.河北大学 基础医学院,河北 保定 071000)摘 要:为了探讨齐墩果酸对衰老大鼠睾丸间质细胞的作用,采用MTT法确定齐墩果酸最适药物浓度,氧化应激创建大鼠睾丸间质细胞衰老模型,RT-qPCR、免疫荧光和免疫印迹检测胰岛素/胰岛素样生长因子1(insulin-like growth factor 1, IGF1)信号通路关键基因转录情况和蛋白翻译情况,采用INSR抑制剂处理细胞,流式细胞术检测细胞凋亡情况.结果显示:与正常组相比,INSR、IRS1、IRS2、IGF1的mRNA转录水平和蛋白翻译水平显著下降(P<0.05);IGFBP3的mRNA转录水平显著提高(P<0.05),免疫荧光和免疫印迹均未检测到此基因表达;与衰老组相比,齐墩果酸组明显逆转此现象(P<0.05);齐墩果酸能明显抑制衰老组细胞的凋亡(P<0.05),INSR抑制剂处理后,细胞凋亡率明显高于齐墩果酸组(P<0.05).综上可知,齐墩果酸通过调控胰岛素/IGF1信号通路关键基因的转录和翻译延缓Leydig细胞衰老.关键词:齐墩果酸;胰岛素/IGF1;衰老;睾丸间质细胞;RT-qPCR中图分类号:Q291 文献标志码:A 文章编号:1000-1565(2022)04-0403-08Effects of oleanolic acid on the expression of key genes of insulin/IGF1 pathway in leydig cells of aging ratsLI Shaohua^1,2, NIU Shuang², DONG Ziyi²,SONG Yicheng², NIU Siyun²(1. Department of Neonatology, Affiliated Hospital of Hebei University, Baoding 071000, China;2. School of Basic Medical Sciences, Hebei University, Baoding 071000, China)Abstract: To investigate the effect of oleanolic acid on leydig cells of aging rats, the MTT method was used to determine the optimal drug concentration of oleanolic acid. The aging module of rat leydig cell was built according to the free radical oxidative damage method. Gene and protein expression of the key gene in the insulin/insulin-like growth factor 1(IGF1)signaling pathway was detected by RT-qPCR, immunofluorescence technique(IF), and Western blot. After treated with INSR inhibitor, cell apoptosis was performed by flow cytometry. The results showed that INSR, IRS1, IRS2 and IGF1 mRNA transcription level and protein expression level were significantly downregulated(P<0.05); IGFBP3 mRNA transcription level was significantly upregulated(P<0.05), otherwise, the expression could not be detected by IF and Western blot. Oleanolic acid reversed the above phenomenon, compared to aging group(P<0.05).- 收稿日期:2021-11-02 基金项目:国家自然科学基金资助项目(81673714);河北省中医药管理局科研项目(2016204;20200234);河北大学研究生创新资助项目(HBU2021ss025) 第一作者:李少华(1980—),女,河北定州人,河北大学附属医院主管护师,主要从事生殖衰老方向研究. E-mail:xiaolizhu2007@163.com 通信作者:牛嗣云(1967—),女,河北承德人,河北大学教授,博士,博士生导师,主要从事生殖衰老方向研究.E-mail: nsy1688@163.com第4期李少华等:齐墩果酸对衰老大鼠睾丸间质细胞胰岛素/IGF1通路关键基因表达的影响Oleanolic acid can delay cell apoptosis in the aging group(P<0.05). It was found that cell apoptosis rate was significantly higher than oleanolic acid group(P<0.05)after treated with INSR inhibitor. In summary, oleanolic acid delays leydig cell senescence by regulating insulin/IGF1 signaling pathway essential gene expression and transcripts.

Cloning and expression of Actinokineospora alba DSM 45114^T rpf gene and structure prediction of Rpf protein

ZHANG Yufan, LIU Jingjing, CAO Min, LIU Tongtong, CHEN Xue, ZHANG Xiumin

2022, 42(4):  411-420.  DOI: 10.3969/j.issn.1000-1565.2022.04.011

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Resuscitation-promoting factor(Rpf)is widely present in gram-positive bacteria with high G+C content and is related to the resuscitation of dormant cells. There are some differences in the types and quantities of Rpf among different bacteria. In order to understand the difference of rpf genes in different species, bioinformatics analysis was conducted on the amino acid sequence, hydrophobicity, signal peptide, phosphorylation site, tertiary structure and structural functional domain of three Rpf proteins in Actinokineospora alba DSM 45114^T. The specific primers for the three rpf genes of A. alba- DOI:10.3969/j.issn.1000-1565.2022.04.011白色放线动孢菌 DSM 45114^T rpf基因的克隆表达及结构预测张宇帆,刘菁菁,曹敏,刘彤彤,陈雪,张秀敏(河北大学 生命科学学院,生命科学与绿色发展研究院,河北省微生物育种与保育工程实验室, 河北省微生物多样性研究与应用重点实验室, 河北 保定 071002)摘 要:复苏促进因子(Rpf)广泛存在于高 G+C 含量的革兰氏阳性细菌中,与休眠细胞的复苏有关,且不同细菌Rpf的种类和数量有一定差异.为了解不同物种中rpf基因的差别,对白色放线动孢菌(Actinokineospora alba)DSM 45114^T的3个Rpf蛋白的氨基酸序列、疏水性、信号肽、磷酸化位点、三级结构以及结构功能域进行生物信息学预测分析.设计了白色放线动孢菌(A. alba)DSM 45114^T rpf基因的特异性引物,从其基因组中扩增出了3个不同的rpf基因,随后构建pET-28a(+)-rpf表达载体,在Escherichia coli BL21(DE3)中进行诱导表达,成功表达了Rpf-2蛋白.将重组Rpf-2蛋白加入到活的非可培养(viable but non culturable,VBNC)状态的藤黄微球菌(Micrococcus luteus)和滨海红球菌(Rhodococcus marinonascens)中,结果表明,重组Rpf-2蛋白可以促进其复苏,添加量在体积分数为10%时复苏效果最好.本研究为后续探讨白色放线动孢菌(A. alba)Rpf蛋白的功能奠定了基础.关键词:白色放线动孢菌;复苏促进因子;异源表达;结构预测中图分类号:Q93 文献标志码:A 文章编号:1000-1565(2022)04-0411-10Cloning and expression of Actinokineospora alba DSM 45114^T rpf gene and structure prediction of Rpf proteinZHANG Yufan, LIU Jingjing, CAO Min, LIU Tongtong, CHEN Xue, ZHANG Xiumin(Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province,Institute of Life Science and Green Development, School of Life Sciences, Hebei University, Baoding 071002, China)Abstract: Resuscitation-promoting factor(Rpf)is widely present in gram-positive bacteria with high G+C content and is related to the resuscitation of dormant cells. There are some differences in the types and quantities of Rpf among different bacteria. In order to understand the difference of rpf genes in different species, bioinformatics analysis was conducted on the amino acid sequence, hydrophobicity, signal peptide, phosphorylation site, tertiary structure and structural functional domain of three Rpf proteins in Actinokineospora alba DSM 45114^T. The specific primers for the three rpf genes of A. alba- 收稿日期:2022-01-03 基金项目:河北省重点研发计划项目(20326614D);河北省自然科学基金资助项目(C2020201005);河北大学2021年度研究生创新资助项目(HBU2021ss051) 第一作者:张宇帆(1997—),女,山西临汾人,河北大学在读硕士研究生,主要从事放线菌资源挖掘研究.E-mail:627256284@qq.com 通信作者:张秀敏(1970—),女,河北涞水人,河北大学教授,博士,主要从事微生物多样性及资源开发研究.E-mail:zhxiumin1106@126.com第4期张宇帆等:白色放线动孢菌 DSM 45114^T rpf基因的克隆表达及结构预测DSM 45114^T were designed, and corresponding genes were amplified. Subsequently, pET-28a(+)-rpf expression vectors were constructed and induced in Escherichia coli BL21(DE3), Rpf-2 protein was successfully expressed. Adding the recombinant Rpf-2 protein to the cells in viable but non culturable(VBNC)state of Micrococcus luteus and Rhodococcus marinonascens, the result showed that it could significantly promote their resuscitation, and the resuscitation effect was the most significant when 10% Rpf-2 protein was added. This laid a foundation for the subsequent study on the function of Rpf-2 protein in A. alba.

A new record of the genus Hesperus from China(Coleoptera: Staphylinidae)

XU Wang, ZENG Qinghuai, TANG rong, CAI Yujie

2022, 42(4):  421-423.  DOI: 10.3969/j.issn.1000-1565.2022.04.012

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In a survey of rove beetles in Wutongshan Nature Reserve of Guangdong Province, a new record species was reported from China: Hesperus laevigatus Fauvel, 1895. The diagnostic characteristics and adult habitus of this species is described and illustrated in this paper.

Analysis of community structure of macrobenthic invertebrates in Baiyangdian Basin

WU Zhixin, WANG Xi, LIU Yuxuan, SUN Xiaoqiong, WANG Hongwei

2022, 42(4):  424-432.  DOI: 10.3969/j.issn.1000-1565.2022.04.013

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The distribution and community structure changes of macrobenthic invertebrates in Baiyangdian Lake, two upstream reservoirs and four upstream rivers were investigated in different seasons in May, August and October in 2019. The dominant species were all pollution-resistant species in Baiyangdian Basin. The biomass mainly showed that the south was higher than the north in Baiyangdian Lake. The biodiversity of macrobenthic invertebrates showed that the central region was higher than the coastal region in Baiyangdian Lake. The changes of biomass and abundance in the two reservoirs were similar, which were higher in spring and autumn than in summer. Fuhe River, Xiaoyihe River and Jumahe River showed the lowest biomass in spring, while Shahe River had the highest biomass in spring. The biodiversity analysis of the four rivers showed that the biodiversity in spring was higher than that in other seasons. In a word, in terms of biomass and biodiversity, the upstream rivers and reservoirs mainly show significant differences in different seasons, while the differences between different regions are not significant.- DOI:10.3969/j.issn.1000-1565.2022.04.013白洋淀流域大型底栖无脊椎动物群落结构分析武志鑫,王玺,刘瑀璇,孙枭琼,王宏伟(河北大学 生命科学学院,生命科学与绿色发展研究院,河北 保定 071002)摘 要:2019年5月、8月和10月对白洋淀流域的淀区、2个上游水库和4条上游河流中大型底栖无脊椎动物的不同季节群落结构变化进行了调查,发现其优势物种均为耐污种.淀区群落结构主要表现出受区域性影响较大,受季节性影响不大的特点,分析其生物量和生物多样性指数,呈现出淀区南部生物量高于淀区北部,淀区中心生物多样性高于淀区边缘;2个水库生物量与丰度变化具有一定的相似性,均表现为春季和秋季高于夏季;府河、孝义河和拒马河表现为春季生物量最低,沙河春季生物量最高,在4条河流中春季生物多样性均高于夏、秋季.总之,从生物量和生物多样性两方面来看,上游河流和水库主要表现为不同季节间差异显著,而不同区域间差异不显著.关键词:白洋淀流域;大型底栖无脊椎动物;群落结构中图分类号:Q89 文献标志码:A 文章编号:1000-1565(2022)04-0424-09Analysis of community structure of macrobenthic invertebrates in Baiyangdian BasinWU Zhixin, WANG Xi, LIU Yuxuan, SUN Xiaoqiong, WANG Hongwei(Institute of Life Science and Green Development,School of Life Sciences, Hebei University, Baoding 071002, China )Abstract: The distribution and community structure changes of macrobenthic invertebrates in Baiyangdian Lake, two upstream reservoirs and four upstream rivers were investigated in different seasons in May, August and October in 2019. The dominant species were all pollution-resistant species in Baiyangdian Basin. The biomass mainly showed that the south was higher than the north in Baiyangdian Lake. The biodiversity of macrobenthic invertebrates showed that the central region was higher than the coastal region in Baiyangdian Lake. The changes of biomass and abundance in the two reservoirs were similar, which were higher in spring and autumn than in summer. Fuhe River, Xiaoyihe River and Jumahe River showed the lowest biomass in spring, while Shahe River had the highest biomass in spring. The biodiversity analysis of the four rivers showed that the biodiversity in spring was higher than that in other seasons. In a word, in terms of biomass and biodiversity, the upstream rivers and reservoirs mainly show significant differences in different seasons, while the differences between different regions are not significant.- 收稿日期:2021-11-02 基金项目: 中国水产科学研究院资助项目(2130135);教育部留学回国基金资助项目(201808130270); 河北省自然科学基金资助项目(C2019201390);河北省留学人员项目(2022);河北大学实验开放项目(sy202091) 第一作者:武志鑫(1994—),女,山东菏泽人,河北大学在读硕士研究生,主要从事水生生物研究.E-mail: wzx121207@163.com 通信作者:王宏伟(1970—),女,河北张家口人,河北大学教授,博士,主要从事水生生物环境监测与保护研究.E-mail: whw6688@126.com第4期武志鑫等:白洋淀流域大型底栖无脊椎动物群落结构分析

UFMC channel estimation algorithm based on improved LMMSE

LIU Zichang, HAO Yulian, ZHANG Suoliang

2022, 42(4):  433-437.  DOI: 10.3969/j.issn.1000-1565.2022.04.014

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Aiming at the deficiency that the prior channel correlation matrix information is required when the linear minimum mean square error(LMMSE)algorithm is directly applied to the universal filtered multi-carrier(UFMC)system, an improved algorithm based on LMMSE is proposed in this thesis. The algorithm smooths the channel frequency response obtained by the least square(LS)algorithm in the frequency domain, determines the ideal channel sampling length by optimizing the truncation of sampling points in the time domain, the optimal channel correlation matrix is obtained by iterative estimation method to avoid prior channel correlation matrix information. The MATLAB simulation verifies that the improved algorithm can effectively suppress the interference of noise. Compared with the traditional LMMSE algorithm, the bit error performance can be improved by about 2 dB, which can effectively improve the accuracy of channel estimation.

A belief network model for microblog recommendation

ZHAO Kaihua, XU Jianmin, BAO Caiqian

2022, 42(4):  438-448.  DOI: 10.3969/j.issn.1000-1565.2022.04.015

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Considering that the existing microblog recommendation methods or models are inconvenient to combine evidence, this paper proposes a new microblog recommendation method. The belief network is applied to microblog recommendation, a basic belief network recommendation model is then constructed, and an extended microblog recommendation model is proposed by integrating the user interaction microblog evidence into the basic model. The nodes in the model represent microblogs, users and interest feature words, while the directed arcs represent relationships between nodes. The relevance between user and microblog is obtained by calculating the degree of coverage between user and microblog. The experimental results on microblog datasets show that the F-measure of the extended model is at least 4.9% higher than that of the basic model. Compared with existing recommendation methods, the new model is more effective in improving recommendation performance in terms of combining evidence.