磁性石墨烯-液质联用测定鸡肉中万古霉素和喹诺酮

doi:10.3969/j.issn.1000-1565.2016.05.006

河北大学学报(自然科学版) ›› 2016, Vol. 36 ›› Issue (5): 480-486.DOI: 10.3969/j.issn.1000-1565.2016.05.006

磁性石墨烯-液质联用测定鸡肉中万古霉素和喹诺酮

董彦莉¹,刘卉闵¹,李超¹,马晶军¹,崔晓娜²

收稿日期:2015-03-15 出版日期:2016-09-25 发布日期:2016-09-25
作者简介:董彦莉(1981—),女,河北鹿泉人,河北农业大学副教授,主要从事分析化学方面的研究. E-mail:dyl19810314@126.com
基金资助:
河北省科技支撑计划项目(14227115D);山东省潍坊市科技支撑计划项目(2015GX044)

Determination of vancomycin and QNs residues in chicken by graphene based-MSPE-liquid chromatography-high-resolution mass spectrometry

DONG Yanli¹,LIU Huimin¹,LI Chao¹,MA Jingjun¹,CUI Xiaona²

1.College of Science, Agricultural University of Hebei, Baoding 071001, China; 2.Department of Food and Drug Science and Technology, Shandong Vocational AnimalScience and Vetevinary College, Weifang 261061, China

Received:2015-03-15 Online:2016-09-25 Published:2016-09-25

摘要/Abstract

摘要： 建立了鸡肉中万古霉素和喹诺酮类药物残留的磁性石墨烯固相萃取-液相色谱-串联质谱分析方法.样品采用乙腈提取,磁性石墨烯粉分散固相萃取净化,经Agilent ZORBAX SB-C18(3.0 mm ×100.0 mm,1.8 μm)色谱柱分离,液相色谱-串联质谱仪进行检测.目标物在2.0~50.0 μg/L内线性关系良好(r²>0.99).鸡肉基质中目标化合物在5、10、25 μg/kg加标水平的回收率为71.2%~98.8%,相对标准偏差(RSD)为6.8%~14.3%.该方法净化效果好,回收率高,精密度好,可用于鸡肉中万古霉素和喹诺酮类药物残留检测.

关键词: 磁性石墨烯固相萃取(MSPE), 液质联用法, 万古霉素, 喹诺酮类

Abstract: A method for the determination of vancomycin and quinolones residues in chicken was developed using liquid chromatography mass spectrometry(LC-MS).The sample was extracted with acetonitrile and purified using MSPE as the sorbent.The target compounds were separated on a Agilent ZORBAX SB-C18 column(3.0 mm×100 mm,1.8 μm)before submitting for detection by high-resolution mass spectrometry.The results showed that high correlation coefficients(r²>0.99)of the analyses were obtained within their respective linear ranges.The average recoveries and the relative standard deviations ranged from 71.2% to 98.8% and 6.8% to 14.3% respectively at the spiked levels of 5,10,25 μg/kg in chicken.This method is reliable,and suitable for the determination of vancomycin and quinolones residues in chicken.

Key words: graphene MSPE, liquid chromatography mass spectrometry, vancomycin, quinolones

中图分类号:

O652

董彦莉,刘卉闵,李超,马晶军,崔晓娜. 磁性石墨烯-液质联用测定鸡肉中万古霉素和喹诺酮[J]. 河北大学学报(自然科学版), 2016, 36(5): 480-486.

DONG Yanli,LIU Huimin,LI Chao,MA Jingjun,CUI Xiaona. Determination of vancomycin and QNs residues in chicken by graphene based-MSPE-liquid chromatography-high-resolution mass spectrometry[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(5): 480-486.

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磁性石墨烯-液质联用测定鸡肉中万古霉素和喹诺酮

Determination of vancomycin and QNs residues in chicken by graphene based-MSPE-liquid chromatography-high-resolution mass spectrometry

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