环介导等温扩增技术研究进展

doi:10.3969/j.issn.1000-1565.2021.05.013

摘要/Abstract

摘要： 环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种高效、简便、高特异性、无需热循环设备的核酸扩增技术. 由于LAMP依然具备巨大的改造潜力,所以近些年来,一直有研究改进其技术以期达到更好的扩增检测效果. 本文对LAMP的基本原理、应用领域范围以及技术改良方向进行了综述,为今后该技术的改良与发展提供参考.

关键词: 环介导等温扩增(LAMP), 技术改良, 发展方向

Abstract: Loop-mediated isothermal amplification(LAMP)is an efficient, simple, highly specific nucleic acid amplification technology without thermal cycling equipment.As LAMP still has great potential for- DOI:10.3969/j.issn.1000-1565.2021.05.013环介导等温扩增技术研究进展石磊¹,王曼²,时国强³,董振国³,刘昱²(1.暨南大学食品安全与营养研究院,广东广州 510000;2.河北农业大学生命科学学院,河北保定 071003; 3. 河北三狮生物科技有限公司研发部,河北石家庄 050000)石磊博士,教授,博士生导师,1985年毕业于河北大学生物系并获学士学位,现任暨南大学食品安全与营养研究院院长,河北农业大学生命科学学院特聘兼职教授;国家重大人才工程入选者,国家重点研发计划首席科学家,肉食品安全生产技术国家重点实验室主任,第一届国家食品安全标准审评委员会委员,中国食源性微生物检测技术创新战略联盟副理事长,广东省免疫学会副理事长,广州双螺旋基因技术有限公司董事长;主要从事病原微生物快速诊断方法、临床微生物耐药基因解析及耐药性基因诊断研究;在SCI收录期刊上发表论文100余篇,申请专利26项,其中发明专利25项,实用新型专利1项;先后主持“国家重点研发计划”、“国家973计划项目”、“国家海洋经济创新发展区域示范专项”等10余项国家级项目;2018年获中国人民解放军军队科技进步二等奖,2019年获第四届中国水产学会范蠡科学技术奖技术推广奖、优秀成果奖.摘要:环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种高效、简便、高特异性、无需热循环设备的核酸扩增技术. 由于LAMP依然具备巨大的改造潜力,所以近些年来,一直有研究改进其技术以期达到更好的扩增检测效果. 本文对LAMP的基本原理、应用领域范围以及技术改良方向进行了综述,为今后该技术的改良与发展提供参考. 关键词:环介导等温扩增(LAMP);技术改良;发展方向中图分类号:Q7 文献标志码:A 文章编号:1000-1565(2021)05-0565-07Research progress of loop-mediated isothermal amplification technologySHI Lei¹, WANG Man², SHI Guoqiang³, DONG Zhenguo³, LIU Yu²(1. Institute of Food Safety and Nutrition,Jinan University, Guangzhou 510000, China; 2. College of Life Science, Agricultural University of Hebei, Baoding 071003, China; 3.Research and Development Department,Hebei Sanshi Biological Technology Co., LTD, Shijiazhuang 050000, China)Abstract: Loop-mediated isothermal amplification(LAMP)is an efficient, simple, highly specific nucleic acid amplification technology without thermal cycling equipment.As LAMP still has great potential for- 收稿日期:2021-06-16 基金项目:河北省创新能力提升计划项目(205A7601D);石家庄市高层次科技创新创业人才项目(05202001) 第一作者:石磊(1961—),男,河北保定人,暨南大学教授,博士生导师,主要从事病原微生物核酸分子检测等研究. E-mail:leishi88@139.com 通信作者:刘昱(1990—),男,山东临沂人,河北农业大学在读博士研究生,主要从事病原微生物核酸分子检测的研究.E-mail:1085869940@qq.com第5期石磊等:环介导等温扩增技术的研究进展modification, many studies have been conducted in recent years to improve its technology in order to achieve better amplification detection results.In this paper, the basic principle, application scope and technical improvement direction of LAMP were reviewed, to provide references for the improvement and development of LAMP technology in the future.

Key words: LAMP, technical improvement, research progress

中图分类号:

石磊,王曼,时国强,董振国,刘昱. 环介导等温扩增技术研究进展[J]. 河北大学学报(自然科学版), 2021, 41(5): 565-571.

SHI Lei, WANG Man, SHI Guoqiang, DONG Zhenguo, LIU Yu. Research progress of loop-mediated isothermal amplification technology[J]. Journal of Hebei University(Natural Science Edition), 2021, 41(5): 565-571.

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