Optimize the Factors Influencing the Spore Germination and Hyphal Growth of Glomus reticulatum

doi:10.3969/j.issn.1000-1565.2010.02.016

Abstract

Abstract: In order to determine the optimal level condition of spore germination and hyphal growth, several factors that influence spores germination and hyphal growth of arbuscular mycorrhizal fungi (AMF) were optimized by L_(16)(4~5) multifactor orthogonal experiment and the best level of spore germination was determined. The results showed that the best technical conditions were as following: pH 8, 10 g/L of water agar, 10 days of culture cycle, 20 days of aging storage, 28 ℃ of culture temperature. We found not only the spore germination was earlier and the germination rate of spores was higher, but also the hyphal growth was more stronger under the optimal condition.

Key words: orthogonal experiment, the germination rate of spores, the growth of hyphal, the rate of hyphal branching

CLC Number:

Q939.5

HE Xue-li,QIAN Wei-hua,XU Zhu-xing. Optimize the Factors Influencing the Spore Germination and Hyphal Growth of Glomus reticulatum[J]. Journal of Hebei University (Natural Science Edition), 2010, 30(2): 185-189.

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