Optimization of ultrasound extraction technology with acid of emodin from herba dianthi by response surface methodology

doi:10.3969/j.issn.1000-1565.2017.04.007Box-Behnken法优化加酸超声

Abstract

Abstract: Response surface methodology was used to optimize the optimal ultrasound extraction technology of emodin from herba dianthi.With the amount of extraction of emodin as an indicator, single-factor test was used to investigate the effect of ethanol volume fraction, solid-liquid ratio, ultrasound time, temperature, mass fraction of hydrochloric acid and dosage on the amount of extraction of emodin.The results were combined with Box-Benhken design of design-expert software and response surface analysis to optimize the technology parameters for extraction process of herba dianthi, and compare with extraction technology of emodin from Polygonum cuspidatum in the Pharmacopoeia.Obtain the optimal ultrasound extraction technology under the following conditions: liquid- solid ratio was 61, 40 ℃,30 min, hydrochloric acid mass fraction 8%, 1 mL.Ultrasound extraction technology of emodin by response surface methodology is time-saving, stable, and the amount of extraction is higher than condensation reflux method.It’s provide theoretical basis for quality evaluation of Dianthus superbus.

Key words: emodin, ultrasound extraction technology, response surface methodology

CLC Number:

R917

YANG Xiaoyu, LI Cheng, MA Siyue, LI Jianheng. Optimization of ultrasound extraction technology with acid of emodin from herba dianthi by response surface methodology[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(4): 369-377.

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