Comparative evaluation of the active ingredients and pharmacological activities of ordinary P. notoginseng powder and lyophilized P. notoginseng powder

doi:10.3969/j.issn.1000-1565.2020.03.007

Abstract

Abstract: To compare the active ingredients and pharmacological activities of ordinary Panax notoginseng powder and freeze-dried P. notoginseng powder. The differences of active ingredients between the ordinary and the freeze-dried P. notoginseng powder were determined by observing the particle size of ordinary powder and freeze-dried P. notoginseng powder under the microscope, visually observing the solubility of the suspension and detecting the active ingredient content by HPLC. The differences of pharmacological effects between ordinary powder and freeze-dried P. notoginseng powder were determined by capillary tube method, tail-cut method, xylene-induced ear swelling test and organ index. The results showed that these two P. notoginseng powders were different in the particle size, solubility and active ingredient content;ordinary powder had strong effect on blood coagulation and lyophilized powder exhibited anti-inflammatory effect;- DOI:10.3969/j.issn.1000-1565.2020.03.007普通和冻干三七粉的有效成分及药理作用比较张君,孙思勉,赵琳,周航,林冬菊,霍小位 (河北大学药学院,河北省药物质量分析控制重点实验室,河北保定 071002)摘要:为比较普通三七Panax notoginseng粉和冻干三七粉的有效成分及药理活性,通过在显微镜下观察普通三七粉和冻干三七粉的粒径,肉眼观察其混悬液的溶解度,HPLC法检测有效成分含量来明确普通三七粉和冻干三七粉在有效成分方面的区别.通过毛细玻管法、剪尾法、二甲苯所致小鼠耳肿胀实验和器官指数等实验研究普通三七粉和冻干三七粉在药理作用方面的差异.研究结果表明:2种三七粉的粒径、溶解度、有效成分含量存在差异.普通粉高剂量组有一定的凝血作用;冻干粉高剂量组有一定的抗炎作用,2者对免疫器官均无明显影响.关键词:三七;冻干粉;普通粉;有效成分;药理作用中图分类号:R96;R917 文献标志码:A 【additional_page=282】文章编号:1000-1565(2020)03-0269-07Comparative evaluation of the active ingredients and pharmacological activities of ordinary P. notoginseng powder and lyophilized P. notoginseng powderZHANG Jun, SUN Simian, ZHAO Lin, ZHOU Hang, LIN Dongju, HUO Xiaowei(Key Laboratory of Pharmaceutical Quality Control of Hebei Province,College of Pharmaceutical Science, Hebei University, Baoding 071002, China)Abstract: To compare the active ingredients and pharmacological activities of ordinary Panax notoginseng powder and freeze-dried P. notoginseng powder. The differences of active ingredients between the ordinary and the freeze-dried P. notoginseng powder were determined by observing the particle size of ordinary powder and freeze-dried P. notoginseng powder under the microscope, visually observing the solubility of the suspension and detecting the active ingredient content by HPLC. The differences of pharmacological effects between ordinary powder and freeze-dried P. notoginseng powder were determined by capillary tube method, tail-cut method, xylene-induced ear swelling test and organ index. The results showed that these two P. notoginseng powders were different in the particle size, solubility and active ingredient content;ordinary powder had strong effect on blood coagulation and lyophilized powder exhibited anti-inflammatory effect;- 收稿日期:2019-07-20 基金项目:河北省教育厅资助项目(QN2018153);河北大学大学生创新创业训练计划项目(2018116,2018111);河北大学研究生创新项目(hbu2019ss082) 第一作者:张君(1993—),女,河北保定人,河北大学在读硕士研究生,主要从事药理学方向的研究.E-mail:zhangjun696@126.com 通信作者:霍小位(1988—),女,山东滨州人,河北大学讲师,主要从事药理学方向的研究.E-mail:huoxiaoweiforever@163.com林冬菊(1987—),女,黑龙江哈尔滨人,河北大学讲师,主要从事药物分析方向的研究.E-mail:lindo.ngju@163.com第3期张君等:普通和冻干三七粉的有效成分及药理作用比较they both had no significant effect on immune organs.

Key words: P. notoginseng, lyophilized powder, ordinary powder, active ingredient, pharmacological action

CLC Number:

R96
R917

ZHANG Jun, SUN Simian, ZHAO Lin, ZHOU Hang, LIN Dongju, HUO Xiaowei. Comparative evaluation of the active ingredients and pharmacological activities of ordinary P. notoginseng powder and lyophilized P. notoginseng powder[J]. Journal of Hebei University (Natural Science Edition), 2020, 40(3): 269-275.

References

[1] 杨娟, 袁一怔, 尉广飞, 等. 三七植物化学成分及药理作用研究进展[J]. 世界科学技术: 中医药现代化, 2017, 19(10): 1641-1647. DOI:10.11842/wst.2017.10.009.
[2] 马妮, 高明菊, 周家明, 等. 不同干燥方法对三七切片皂苷含量的影响[J]. 特产研究, 2010, 32(3): 40-42. DOI:10.16720/j.cnki.tcyj.2010.03.018.
[3] 吴孟华, 张英, 马志国, 等. 三七炮制的历史沿革考证[J].中国中药杂志, 2018, 43(24): 4923-4928. DOI:10.19540/j.cnki.cjcmm.20181025.007.
[4] 徐成海, 张世伟, 彭润玲, 等. 真空冷冻干燥的现状与展望(二)[J]. 真空, 2008(03): 1-13. DOI:10.13385/j.cnki.vacuum.2008.03.008.
[5] 鲍明慧, 宋爱刚, 牟希全. 冻干技术及其在药品生产中的应用[J]. 化工装备技术, 2002, 23(6): 4-9. DOI:10.16759/j.cnki.issn.1007-7251.2002.06.002.
[6] 乔晓玲, 闫祝炜, 张原飞, 等. 食品真空冷冻干燥技术研究进展[J]. 食品科学, 2008(05): 469-474. DOI:10.3321/j.issn:1002-6630.2008.05.107.
[7] 刘勇, 陈骏飞, 徐娜, 等. 趁鲜切制加工对三七药材干燥速率和质量的影响[J]. 中国中药杂志, 2019, 44(07): 1381-1391. DOI:10.19540/j.cnki.cjcmm.20181221.002.
[8] 何志鹏. 制药行业中真空冷冻干燥技术的应用[J]. 农村经济与科技, 2017, 28(18): 242-242. DOI:10.3969/j.issn.1007-7103.2017.18.162.
[9] 刘嘉, 刘汉清. 冻干技术及其在中药冻干制剂中应用的研究进展[J]. 中国医药技术经济与管理, 2007, 1(5): 38-42.
[10] 曾莹, 陈颖, 伍善广. 真空冷冻干燥技术在提高药物新剂型稳定性中的应用[J]. 海峡药学, 2017, 29(8): 4-6.
[11] 闫家福, 仝燕, 王锦玉, 等. 冷冻干燥技术及其在中药研究中的应用[J]. 中国实验方剂学杂志, 2006, 12(12): 65-69. DOI:10.13422/j.cnki.syfjx.2006.12.024.
[12] 周国燕, 詹博, 桑迎迎, 等. 不同干燥方法对三七内部结构和复水品质的影响[J]. 食品科学, 2011, 32(20): 44-47.
[13] 梁志国, 张秋生. 浅谈冻干技术应用[J]. 黑龙江科技信息, 2009(6): 149.
[14] 赵常英. 关于三七的活性成分药理与临床应用功效的相关探讨[J]. 世界最新医学信息文摘, 2016, 16(90): 195-196. DOI:10.3969/j.issn.1671-3141.2016.90.125.
[15] 白俊峰. 冻干三七主要功效及其总皂苷的含量测定[J]. 世界最新医学信息文摘, 2018, 18(79): 136-137. DOI:10.19613/j.cnki.1671-3141.2018.79.089.
[16] 朱婷,冯玉,殷乐章,等. 三七皂苷R1对异丙肾上腺素诱导的H9c2心肌细胞肥大的保护作用[J]. 现代食品科技, 2018, 34(10): 1-7. DOI:10.13982/j.mfst.1673-9078.2018.10.001.
[17] 朱志扬,葛然,杨露露. 人参皂苷Rb1通过抑制PI3K/AKT信号通路保护脂多糖诱导的心肌细胞炎症反应[J]. 中国循证心血管医学杂志, 2018, 10(07): 823-826. DOI:10.3969/j.issn.1674-4055.2018.07.13.
[18] 宋志明,余舒杰,巩贵宏,等. 人参皂苷Rb1通过抑制炎症反应及氧化应激改善老年小鼠内皮功能[J]. 中国实验诊断学, 2018, 22(06): 1066-1070.
[19] 种宗雷,杨晓倩,肖以磊,等. 人参皂苷Rg1对过氧化损伤人脐带间充质干细胞的保护作用及机制[J]. 山东医药, 2018, 58(29): 29-3. DOI:10.3969/j.issn.1002-266X.2018.29.008.