Changes of antioxidant enzyme activity in callus of Hylotelephium tatarinowii under PEG 6 000 stress

doi:10.3969/j.issn.1000-1565.2020.04.012

Abstract

Abstract: Callus of Hylotelephium tatarinowii(Maxim.)H. Ohba was treated by different concentrations of polyethylene glycol(PEG 6 000)for stress treatment in the study. The activity of peroxidase(POD), superoxide dismutase(SOD)and catalase(CAT)in callus was measured, and isozyme expressions of POD, SOD and esterase(EST)were analyzed by non-denatured polypropylene gel electrophoresis. The activity of POD, SOD and CAT increased gradually with the increase of concentration and the prolongation of stress time when the concentration of PEG 6 000 was 0.5%—5.0%, and the growth state of callus was normal. When the concentration of PEG 6 000 was 8%, the activity of POD, SOD and CAT decreased sharply with the prolongation of stress time.

Key words: Hylotelephium tatarinowii(Maxim.)H. Ohba, callus, PEG, stress, enzyme activity

CLC Number:

Q945

HUANG Hangjun, LI Yi, MA Linxi, MA Weiwei, WANG Junli. Changes of antioxidant enzyme activity in callus of Hylotelephium tatarinowii under PEG 6 000 stress[J]. Journal of Hebei University (Natural Science Edition), 2020, 40(4): 415-420.

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