Table of Content

25 July 2020, Volume 40 Issue 4

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Reducibility conditions for fuzzy differential equation

JI Liye, YOU Cuilian

2020, 40(4):  337-343.  DOI: 10.3969/j.issn.1000-1565.2020.04.001

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Fuzzy differential equation is an important tool for studying dynamic system in fuzzy environment. Therefore, finding the solutions to these equations is essential. In order to make more fuzzy differential equations easier to solve, variables substitution is performed in nonlinear fuzzy differential equation to judge whether the equation is reducible. In this process, we try to find out the method to reduce a nonlinear fuzzy differential equation to a linear fuzzy differential equation. In the last, the conditions of two types of fuzzy differential equations being reducible are given. Therefore, it becomes easy to distinguish reducible fuzzy differential equations and solve them. In addition, some examples are given to verify the effectiveness of the results.

An urban traffic congestion analysis based on the interpretive structure model and MICMAC

JIA Fan

2020, 40(4):  344-350.  DOI: 10.3969/j.issn.1000-1565.2020.04.002

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Through the investigation of the current situation of urban traffic congestion, the 11 main factors causing urban traffic congestion are summarized from the three aspects, management, facilities and traffic participants. The model of interpretative structure is established, and the hierarchical relationship among factors is analyzed. The MICMAC analysis classifies the influencing factors, further analyzes the influence of each factor on the traffic congestion, finds the surface, middle and bottom influencing factors that cause the urban traffic congestion, and provides the basis for reference to alleviate the urban traffic congestion problem.

Biasing mechanical properties of self-compacting iron tailings concrete short columns of the square steel tubes

WANG Yuanxi, DU Xikai, LIU Jinghong

2020, 40(4):  351-359.  DOI: 10.3969/j.issn.1000-1565.2020.04.003

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Based on the test of 10 square steel tubes with self-compacting iron tailings concrete eccentric compression short columns, this work analyzed the mechanical properties of the test piece affected by the steel content, iron tailings powder replacement rate, and eccentricity, and compared the test bearing capacity with the calculated bearing capacity of each specification. The results show that, with the increase of steel content, the bearing capacity, ductility coefficient and flexural rigidity of the specimen increase significantly; with the substitution rate of iron tailings powder increases, the bending stiffness of the specimen decreases, and there is no obvious change in the bearing capacity and ductility coefficient; with the eccentricity increases, the bearing capacity and bending stiffness of the specimen decrease while the ductility coefficient increases.

Effect of NaCl concentration on an atmospheric glow discharge activated liquid activation by spectroscopic methods

XU Huimin,JIA Peipei,CHEN Junyu,JIA Pengying,LI Xiaoni

2020, 40(4):  360-364.  DOI: 10.3969/j.issn.1000-1565.2020.04.004

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Atmospheric pressure glow discharge has similar advantages owing to glow discharge obtained at low pressure, high chemical reactivity and being dispensable of expensive vacuum device. In particular, increasing attention is paid to atmospheric pressure glow discharge above liquid surface due to abundance with reactive species, which has extensive application potentials in many fields, including sterilization, plant growth, environmental protection, etc. In this paper, a needle-liquid electrode device with different concentrations of NaCl is used to generate atmospheric pressure glow discharge above solution. Optical emission spectra show that the liquid-electrode discharges with different NaCl concentrations present similar spectral lines, which mainly include the second system of nitrogen molecules(337.1 nm), the first negative system of nitrogen molecular ions(391.4 nm)and O I. However, intensity ratio of spectral lines is different. Furthermore the O I intensity, the intensity ratio(I_391.4/I_337.1)and vibrational temperature of discharge are measured by optical emission- DOI:10.3969/j.issn.1000-1565.2020.04.004光谱法研究盐溶液浓度对辉光放电活化液体的影响许慧敏^1,2,贾培佩¹,陈俊宇³,贾鹏英³,李小妮³(1.河北金融学院 基础教学部,河北 保定 071051;2.华北电力大学 电力工程系,河北 保定 071003;3.河北大学 物理科学与技术学院,河北 保定 071002)摘 要:大气压辉光放电具有低气压辉光放电的均匀性和较高的化学活性,且摆脱了真空装置的限制.液面上的大气压辉光放电除了具有以上优点外,还具有活性粒子浓度高等优势,因此在材料改性、生物医疗等领域具有非常广泛的应用前景.本文采用针-液体电极直流放电装置,在液体电极上方产生了大气压辉光放电,并用于活化不同浓度的NaCl溶液.发射光谱表明,不同浓度液体电极放电均包含N₂的第二正带系、N⁺₂的第一负带系、OH自由基和O I.基于发射光谱,测量了液面附近的氧原子谱线强度、谱线强度比I_391.4/I_337.1和振动温度.利用吸收光谱测量了O₃浓度.发现它们均随着放电电流的增大而增大,且在一定电流下,随盐溶液浓度减小而增大.经过相同活化时间,溶液的H₂O₂浓度随着放电电流的增大而增大.在一定放电电流下,H₂O₂浓度随盐溶液浓度增大而减小.关键词:发射光谱;吸收光谱;溶液浓度;大气压辉光放电;振动温度中图分类号:O539 文献标志码:A 文章编号:1000-1565(2020)04-0360-05Effect of NaCl concentration on an atmospheric glow discharge activated liquid activation by spectroscopic methodsXU Huimin^1,2,JIA Peipei¹,CHEN Junyu³,JIA Pengying³,LI Xiaoni³(1. Department of Basic Teaching, Hebei Finance University, Baoding 071051, China;2.Department of Electrical Engineering, North China Electric Power University, Baoding 071003, China;3.College of Physics Science and Technology, Hebei University, Baoding 071002, China)Abstract: Atmospheric pressure glow discharge has similar advantages owing to glow discharge obtained at low pressure, high chemical reactivity and being dispensable of expensive vacuum device. In particular, increasing attention is paid to atmospheric pressure glow discharge above liquid surface due to abundance with reactive species, which has extensive application potentials in many fields, including sterilization, plant growth, environmental protection, etc. In this paper, a needle-liquid electrode device with different concentrations of NaCl is used to generate atmospheric pressure glow discharge above solution. Optical emission spectra show that the liquid-electrode discharges with different NaCl concentrations present similar spectral lines, which mainly include the second system of nitrogen molecules(337.1 nm), the first negative system of nitrogen molecular ions(391.4 nm)and O I. However, intensity ratio of spectral lines is different. Furthermore the O I intensity, the intensity ratio(I_391.4/I_337.1)and vibrational temperature of discharge are measured by optical emission- 收稿日期:2019-10-31 基金项目:国家自然科学基金资助项目(11575050;11875121;51977057);河北省自然科学基金资助项目(A2016201042);河北省研究生创新资助项目(CXZZBS2019023);保定市科技局科技计划项目(1911ZG014);河北金融学院科研项目(JY2016ZB42) 第一作者:许慧敏(1977—),女,河北博野人,河北金融学院讲师,博士,主要从事高电压技术方向研究.E-mail:xuhuimin@126.com第4期许慧敏等:光谱法研究盐溶液浓度对辉光放电活化液体的影响spectroscopy. Results show that the intensity ratio and vibrational temperature increase with increasing discharge current. For a given current, the intensity ratio and the vibrational temperature decrease with increasing NaCl concentration of liquid electrode. In addition, ozone concentration in gas phase is measured by UV absorption method, which increases with increase of discharge current. For a given current, it decreases with increasing NaCl concentration. In addition, H₂O₂concentration in solution increases with increasing discharge current. For a given current, it decreases with increasing NaCl concentration.

Efficient separation of copper and silicon from waste photovoltaic panels

ZHAO Xinmei, ZHANG Zisheng, LAI Weidong, CHEN Xiaoyu, WU Cuigu, DONG Guoyi

2020, 40(4):  365-371.  DOI: 10.3969/j.issn.1000-1565.2020.04.005

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In order to improve the separation efficiency of copper(Cu)and silicon(Si)in waste photovoltaic panels, the optimal efficiency factors for high-voltage electrostatic separation were explored. Using the single factor impact experiment, the separation efficiency of Cu and Si under the four factors of voltage(U), speed(n), electrode spacing(S)and corona electrode angle(θ)were discussed. And Minitab software was used to screen all-factor experimental design and establish the main effect and interaction of the influencing factors on the separation efficiency of Cu and Si. The separation efficiency of Cu was optimized by response optimization. The optimal separation efficiency of Cu was set to U=20 kV, n=65 r/min, S=90 mm, θ=-10°. Under the optimal settings, verification experiments were carried out. The average separation efficiency of Cu reached 97.91%. The optimization results were obtained.

Synthesis of naphthyl-perimidium based receptor and selective recognition of fluoride

DONG Zhiyun, JIA Jia, WANG Shimei, XI Fugui

2020, 40(4):  372-378.  DOI: 10.3969/j.issn.1000-1565.2020.04.006

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A novel naphthyl-perimidium based receptor 1 was synthesized. The interaction between receptor 1 and nine common anions(F^-, Cl^-, Br^-, I^-, CH₃COO^-, HSO^-₄, H₂PO₄^-, ClO₄^-, NO^-₃)were studied by UV-Vis and FL spectroscopies. The results indicated that receptor 1 should selective recognition of F^-. In UV-Vis spectra, When F^- was added, the color of receptor 1 solution changed from yellow to colorless, the results indicated that 1 could behave as a colorimetric chemosensor for F^-. Job plot demonstrated that receptor 1 formed 1∶1 complex with F^-, and the binding constant is(3.05±0.15)×10³ L/mol. In fluorescent spectra, the non-fluorescent solution of 1 exhibited strong blue fluorescence when F^- was added, and a strong emission peak at 403 nm was found with the turn-on effect. The detection linear range for F^- was 1.0×10^-6—3.0×10^-5 mol/L, and the detection limit was 8.5×10^-6 mol/L. The ¹H NMR investigation confirmed that the hydrogen bond was formed between F^- and the C(2)-H of receptor 1, the excessive of F^- will cause deprotonation of receptor 1.

Identification of three Chinese herbal medicines based on terahertz time-domain spectroscopy

LI Ruikai, HOU Kaixuan, ZHANG Lina, LOU Cunguang, LIU Xiuling

2020, 40(4):  379-384.  DOI: 10.3969/j.issn.1000-1565.2020.04.007

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This paper employs terahertz(THz)time-domain spectroscopy combined with principal component analysis(PCA)and K-means clustering algorithm for the detection of three groups of Chinese herbal medicines with little discrimination in physical form. The results show that the THz time-domain spectrum of the authentic Chinese herbal medicines has a significant delay relative to the reference signal, and the absorption spectrum of three groups of Chinese herbal medicines shows distinct absorption coefficients and refractive index in the range of 0.2—1.5 THz. The obtained spectral absorbance data is subjected to dimensionality reduction processing and is applied to the K-means clustering algorithm. The score map of three sets of authentic Chinese herbal medicines obtained by PCA reaches up to 100%. The- DOI:10.3969/j.issn.1000-1565.2020.04.007基于太赫兹时域光谱的3种中药材真伪鉴别李瑞凯¹,侯凯旋²,张丽娜³,娄存广²,刘秀玲²(1. 河北大学附属医院 信息中心,河北 保定 071002;2. 河北大学 电子信息工程学院,河北省数字医疗工程重点实验室,河北 保定 071002;3. 河北大学 中医学院,河北 保定 071002)摘 要:基于太赫兹时域光谱对3组物理形态区分度很小的中药材进行光谱检测,并结合主成分分析(PCA)与K均值聚类算法(K-means)对光谱数据进行差异性对比. 研究结果表明所测真伪品中药的时域信号相对于参考信号有了明显的延迟,真伪品的频域吸收谱差别明显,3组中药的吸收系数及折射率在0.2~1.5 THz内存在明显差异. 对得到的吸收光谱数据进行降维处理,并应用K均值聚类算法得到主成分得分图,3组真伪中药的差别判断率高达100%. 太赫兹时域光谱结合主成分分析在中药真伪的鉴别中具有很好的应用前景,可以直观地得到真伪中草药的差别,具有快速、稳定、准确、无损的优点,对未来相关便携式太赫兹中药品质检测仪器的开发也具有很高的研究价值. 关键词:太赫兹技术;中药真伪鉴别;光谱分析;主成分分析中图分类号:O433.4 文献标志码:A 文章编号:1000-1565(2020)04-0379-06Identification of three Chinese herbal medicines based on terahertz time-domain spectroscopyLI Ruikai¹, HOU Kaixuan², ZHANG Lina³, LOU Cunguang², LIU Xiuling²(1. Information Center, Affiliated Hospital of Hebei University, Baoding 071002, China;2. Hebei Key Laboratory of Digital Medical Engineering, College of Electronic Information Engineering, Hebei University, Baoding 071002, China;3. College of Traditional Chinese Medicine, Hebei University, Baoding 071002, China)Abstract: This paper employs terahertz(THz)time-domain spectroscopy combined with principal component analysis(PCA)and K-means clustering algorithm for the detection of three groups of Chinese herbal medicines with little discrimination in physical form. The results show that the THz time-domain spectrum of the authentic Chinese herbal medicines has a significant delay relative to the reference signal, and the absorption spectrum of three groups of Chinese herbal medicines shows distinct absorption coefficients and refractive index in the range of 0.2—1.5 THz. The obtained spectral absorbance data is subjected to dimensionality reduction processing and is applied to the K-means clustering algorithm. The score map of three sets of authentic Chinese herbal medicines obtained by PCA reaches up to 100%. The- 收稿日期:2019-12-18 基金项目:国家自然科学基金资助项目(61673158, 11304103, 61473112);河北省高校青年拔尖人才项目(BJ2016005) 第一作者:李瑞凯(1991—),男,河北邢台人,河北大学附属医院工程师,主要从事基于大分子太赫兹光谱的疾病无损检测方向研究. E-mail: 1012193070@qq.com 通信作者:娄存广(1985—),男,山东聊城人,河北大学副教授,主要从事基于大分子太赫兹光谱的疾病无损检测方向研究.E-mail: loucunguang@163.com第4期李瑞凯等:基于太赫兹时域光谱的3种中药材真伪鉴别method of terahertz time-domain spectroscopy combined with PCA has a good application prospect in the identification of Chinese herbal medicine authenticity with the advantages of being fast, stable,accurate and non-destructive. It also has high research value for the development of related portable terahertz instruments for Chinese herbal medicines quality testing.

Effects on SPR of Au/TiO₂ by preparation methods

JIA Qunpeng, MA Zhiling, HAN Bing, CHEN Chunxia, ZHANG Jixuan

2020, 40(4):  385-391.  DOI: 10.3969/j.issn.1000-1565.2020.04.008

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The deposition of nano-gold particles on TiO₂ to produce Au/TiO₂ with surface plasmon resonance effect(SPR)is one of the important ways to improve the usage and quantum efficiency of TiO₂ photocatalyst for solar energy. In this paper, HAuCl₄·3H₂O and P25 nano-TiO₂ as raw materials, the effect of chemical reduction temperature on the SPR of gold sol was studied by UV-vis spectrophotometer; and the SPR difference of Au/TiO₂ prepared by chemical reduction thermal deposition method, chemical reduction photodeposition method and photoreduction deposition method were characterized by UV diffuse reflection. The results show that photoreduction deposition is an efficient, economical and environmentally friendly method for the preparation of high SPR Au/TiO₂.

Effects of liquor-saturated process on the Stellera chamaejasmes anti-tumor activity and toxicity

LI Hongling, SONG Meiyan, MA Haoran, LU Yibei, MA Xiaoli, XU Yanjiao

2020, 40(4):  392-398.  DOI: 10.3969/j.issn.1000-1565.2020.04.009

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The difference in anti-tumor activity and toxic reaction in vivo between the crude medicine and liquor-saturated products was compared, and its preliminary anti-tumor mechanism was studied. Intragastric administration was used to compare and study the inhibitory effects of different concentrations of liquor-saturated and crude of Stellera chamaejasme. solution on mices subcutaneous S180 tumor. Immunohistochemical method was used to detect the expression levels of Ki-67 and Caspase-3 in tumor tissues, and to explore the antitumor mechanism of the drugs. There was a significant difference in tumor inhibition between liquor-saturated group and the crude drug group(P<0.05). Compared with the blank control group, the spleen index and thymus index of the wine group were not statistically difference(P<0.05). liquor-saturated Stellera chamaejasme can significantly down-regulate Ki-67 expression in tumor tissue of S180 tumor-bearing mice(P<0.01), and can significantly up-regulate the expression of Caspase-3(P<0.01). Liquor-saturated process can enhance the Stellera Chamaejasmes anti-tumor activity in S180 transplanted mice, and reduce the Stellera chamaejasmes impact on spleen index of mice. The anti-tumor- DOI:10.3969/j.issn.1000-1565.2020.04.009酒制对瑞香狼毒抗肿瘤活性及毒性反应的影响李红玲¹,宋美燕¹,马昊然¹,陆艺蓓¹,马晓莉²,徐艳娇¹(1. 河北大学 医学院,河北 保定 071000;2.河北大学 中医学院,河北 保定 071000)摘 要:实验对比了瑞香狼毒(stellera chamaejasme)生药和酒制品的体内抑瘤活性及毒性反应差异,并对其抑瘤机制进行了初步探讨.以灌胃的给药方式,对比不同浓度的酒制及生药瑞香狼毒溶液对小鼠S180皮下移植瘤的抑制作用.采用免疫组化法检测肿瘤组织Ki-67、Caspase-3的表达水平,探讨药物的抑瘤作用机制.结果表明:酒制瑞香狼毒组与生药组相比,抑瘤率有显著性差异(P<0.05);与空白对照组相比,酒制组脾指数、胸腺指数无统计学差异(P>0.05);酒制瑞香狼毒组S180荷瘤小鼠肿瘤组织中的Ki-67的表达显著下降(P<0.01),Caspase-3的表达增强(P<0.01).因此,酒制能增强瑞香狼毒对S180荷瘤小鼠的抑瘤活性,同时能减轻生药对小鼠脾指数的影响;其抗肿瘤作用机制可能与S180细胞中Ki-67的表达下降以抑制其增殖,Caspase-3的表达增强以促进细胞凋亡有关.关键词:瑞香狼毒;酒制;Ki-67;Caspase-3中图分类号:R332 文献标志码:A 【additional_page=420】文章编号:1000-1565(2020)04-0392-07Effects of liquor-saturated process on the Stellera chamaejasmes anti-tumor activity and toxicity LI Hongling¹, SONG Meiyan¹, MA Haoran¹, LU Yibei¹, MA Xiaoli², XU Yanjiao¹(1.Medical College,Hebei University,Baoding 071000,China;2.College of Traditional Chinese Medicine,Hebei University,Baoding 071000,China)Abstract: The difference in anti-tumor activity and toxic reaction in vivo between the crude medicine and liquor-saturated products was compared, and its preliminary anti-tumor mechanism was studied. Intragastric administration was used to compare and study the inhibitory effects of different concentrations of liquor-saturated and crude of Stellera chamaejasme. solution on mices subcutaneous S180 tumor. Immunohistochemical method was used to detect the expression levels of Ki-67 and Caspase-3 in tumor tissues, and to explore the antitumor mechanism of the drugs. There was a significant difference in tumor inhibition between liquor-saturated group and the crude drug group(P<0.05). Compared with the blank control group, the spleen index and thymus index of the wine group were not statistically difference(P<0.05). liquor-saturated Stellera chamaejasme can significantly down-regulate Ki-67 expression in tumor tissue of S180 tumor-bearing mice(P<0.01), and can significantly up-regulate the expression of Caspase-3(P<0.01). Liquor-saturated process can enhance the Stellera Chamaejasmes anti-tumor activity in S180 transplanted mice, and reduce the Stellera chamaejasmes impact on spleen index of mice. The anti-tumor- 收稿日期:2019-11-10 基金项目:河北省科技厅资助项目(17272502D) 第一作者:李红玲(1978—),女,河北容城人,河北大学实验师,主要从事中药抗肿瘤研究.E-mail:980374832@qq.com 通信作者:马晓莉(1967—),女,河北定兴人,河北大学教授,主要从事中药药物分析方向研究.E-mail:ma_ xiaoli@163.com第4期李红玲等:酒制对瑞香狼毒抗肿瘤活性及毒性反应的影响mechanism may be related to down-regulation of Ki-67 expression and enhancement of Caspase-3 expression. liquor-saturated Stellera chamaejasme can inhibit the proliferation of S180 tumor cells and promote their apoptosis.

Cloning and expression analysis of CiCesA3 from Caragana intermedia

ZHU Hong, CHAI Wenjuan, YANG Feiyun, WANG Ruigang

2020, 40(4):  399-408.  DOI: 10.3969/j.issn.1000-1565.2020.04.010

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This research aims to figure out the structure and expression pattern of cellulose synthase gene(CesA)in Caragana intermedia. The full-length cDNA of CesA was obtained by homologous cloning and RACE from C. intermedia. NCBI Blast indicated the CesA shared the highest identity with GsCesA3 from Glycine soja(94%), and was named as CiCesA3. The length of CiCesA3 cDNA was 3 475 bp, the open reading frame was 3 225 bp, encoded a polypeptide of 1 075 amino acids. The length of 5 UTR and 3 UTR was 175 and 76 bp, respectively. The predicated molecular weight of CiCesA3 was 119.89 ku,and 16—79 amino acids contained a zinc-finger motif(Cx2Cx12FxACx2CxEx5Gx3Cx2C)which is a conserved- DOI:10.3969/j.issn.1000-1565.2020.04.010中间锦鸡儿CiCesA3的克隆及表达分析朱宏¹,柴文娟²,杨飞芸^{2, 3},王瑞刚 ^{2, 3}(1. 哈尔滨师范大学 生命科学与技术学院,黑龙江 哈尔滨 150080;2. 内蒙古农业大学 植物逆境生理与分子生物自治区重点实验室,内蒙古 呼和浩特 010018;3. 内蒙古自治区植物基因资源挖掘与分子育种工程技术研究中心,内蒙古 呼和浩特 010018)摘 要:为了研究中间锦鸡儿Caragana intermedia纤维素合酶基因(cellulose synthase,CesA)的结构及表达特性,通过同源克隆及RACE(rapid amplification of cDNA end)技术,获得1条中间锦鸡儿CesA基因的全长cNDA序列.NCBI Blast结果显示该基因与野生大豆Glycine soja基因GsCesA3相似度最高(94%),因此命名为CiCesA3.CiCesA3基因cDNA全长为3 475 bp,其中5'UTR长为175 bp,3'UTR长为76 bp,开放阅读框长度为3 225 bp,编码1 075个氨基酸.CiCesA3蛋白预测分子质量为119.89 ku,第16位到第79位氨基酸为CesA蛋白保守的锌指结构(Cx2Cx12FxACx2CxEx5Gx3Cx2C).CiCesA3蛋白C端有6个跨膜结构域,为亲水性蛋白.qRT-PCR检测不同组织中基因表达量的结果表明CiCesA3在叶中表达量最高.以上结果证明中间锦鸡儿CiCesA3基因与其他植物CesA基因相似,并无变异,为阐明中间锦鸡儿纤维素的合成提供了依据.关键词:中间锦鸡儿;纤维素合酶;CiCesA3;基因克隆中图分类号:Q785 文献标志码:A 文章编号:1000-1565(2020)04-0399-10Cloning and expression analysis of CiCesA3 from Caragana intermediaZHU Hong¹, CHAI Wenjuan², YANG Feiyun^{2, 3}, WANG Ruigang^{2, 3}(1. College of Life Sciences and Technology, Harbin Normal University, Harbin 150080,China; 2. Key Laboratory of Plant Stress Physiology and Molecular Biology, Inner Mongolia Agricultural University, Hohhot 010018, China; 3. Inner Mongolia Engineering Research Center for Plant Gene Resources Mining and Molecular Breeding, Hohhot 010018, China)Abstract: This research aims to figure out the structure and expression pattern of cellulose synthase gene(CesA)in Caragana intermedia. The full-length cDNA of CesA was obtained by homologous cloning and RACE from C. intermedia. NCBI Blast indicated the CesA shared the highest identity with GsCesA3 from Glycine soja(94%), and was named as CiCesA3. The length of CiCesA3 cDNA was 3 475 bp, the open reading frame was 3 225 bp, encoded a polypeptide of 1 075 amino acids. The length of 5 UTR and 3 UTR was 175 and 76 bp, respectively. The predicated molecular weight of CiCesA3 was 119.89 ku,and 16—79 amino acids contained a zinc-finger motif(Cx2Cx12FxACx2CxEx5Gx3Cx2C)which is a conserved- 收稿日期:2019-11-16 基金项目:内蒙古自治区科技计划项目(2019GG007);内蒙古自然科学基金重大项目(2019ZD05);内蒙古自治区科技创新引导项目(KCBJ2018012) 第一作者:朱宏(1968—),女,黑龙江齐齐哈尔人,哈尔滨师范大学教授,博士,主要从事植物细胞生物学研究.E-mail: zhuhong10000@163.com 通信作者:王瑞刚(1972—),男,内蒙古赤峰人,内蒙古农业大学教授,博士生导师,主要从事植物生物化学与分子生物学研究.E-mail: ruigangwang@126.com第4期朱宏等:中间锦鸡儿CiCesA3的克隆及表达分析motif of CesA. CiCesA3 was a hydrophilic protein, and six transmembrane domains existed in the C-terminal. Real-time quantitative RT-PCR of CiCesA3 in C. intermedia different tissues showed the highest expression level of CiCesA3 was from leaf. The results demonstrated that CiCesA3 was similar to CesA of other plants, and provide some foundation to clarify the cellulose synthesis of C. intermedia.

Determination of absolute configuration of Artemisia annua α-bisabolol synthase products

MU Xing, YANG Qian, LIU Huiyun, XIANG Ying, CAO Fei, LI Zhenqiu

2020, 40(4):  409-414.  DOI: 10.3969/j.issn.1000-1565.2020.04.011

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The gas and liquid chromatography method were established to identify the absolute configuration of α-bisabolol and to determine the absolute configuration of Artemisia annua α-bisabolol synthase products. Separation and preparation of four stereoisomers of α-bisabolol were performed by chiral liquid phase. The absolute configurations of four kinds of stereoisomers were determined by gas chromatography and standard, combined with optical rotation determination. Identification of four stereoisomers of α-bisabolol by gas and liquid chromatography, chemical synthesis of substrate farnesyl pyrophosphate(FPP). By prokaryotic expression, protein purification and in vitro catalysis, the absolute configuration of two bisabolol synthases in vitro was identified. Consistent with the literature report, Lippia dulcis α-bisabolol- DOI:10.3969/j.issn.1000-1565.2020.04.011青蒿α-bisabolol合酶产物绝对构型的确定穆星¹,杨倩¹,刘会云¹,向英¹,曹飞²,李振秋¹(1.河北大学 生命科学学院,河北 保定 071002;2. 河北大学 药学院,河北 保定 071002)摘 要:建立气相和液相色谱方法用于鉴定α-没药醇(α-bisabolol)绝对构型,确定青蒿α-bisabolol合酶产物的绝对构型. 手性液相方法分离并制备α-bisabolol的4种立体异构体,结合旋光度测定、气相色谱和标准品确定4种立体异构体的绝对构型. 分别建立气相和液相色谱2种方法来鉴定α-bisabolol的4种立体异构体混合物. 化学合成酶促反应的底物法尼基焦磷酸(farnesyl pyrophosphate,FPP),原核表达、蛋白纯化后进行体外催化,鉴定2个α-bisabolol合酶体外催化产物α-bisabolol的绝对构型. 甜舌草α-bisabolol合酶(Lippia dulcis bisabolol synthase, LdBOS)体外催化FPP,产物构型为(+)-epi-α-bisabolol,与文献报道一致. 青蒿α-bisabolol合酶(Artemisia annua bisabolol synthase, AaBOS)体外催化FPP生成的产物为(+)-α-bisabolol. Agilent CP-Chirasil-Dex-CB气相色谱柱和Chiralpak IA液相色谱柱可分别用以分离、鉴定α-bisabolol的4种手性异构体. AaBOS和LdBOS的催化产物6位碳构型相同,暗示这2种酶与青蒿紫穗槐二烯合酶(Artemisia annua amorpha-4,11-diene synthase, AaADS)的催化机理类似. 利用气相和液相色谱的方法分析手性化合物,具有灵敏度高、稳定性强、简便易行等优点. 手性化合物α-bisabolol绝对构型的分离、鉴定,在医药、食品和化工领域具有重要意义.关键词:没药醇合酶;倍半萜;青蒿;立体异构体;α-没药醇中图分类号:Q785 文献标志码:A 文章编号:1000-1565(2020)04-0409-06Determination of absolute configuration of Artemisia annua α-bisabolol synthase productsMU Xing¹, YANG Qian¹, LIU Huiyun¹, XIANG Ying¹, CAO Fei², LI Zhenqiu¹(1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China )Abstract: The gas and liquid chromatography method were established to identify the absolute configuration of α-bisabolol and to determine the absolute configuration of Artemisia annua α-bisabolol synthase products. Separation and preparation of four stereoisomers of α-bisabolol were performed by chiral liquid phase. The absolute configurations of four kinds of stereoisomers were determined by gas chromatography and standard, combined with optical rotation determination. Identification of four stereoisomers of α-bisabolol by gas and liquid chromatography, chemical synthesis of substrate farnesyl pyrophosphate(FPP). By prokaryotic expression, protein purification and in vitro catalysis, the absolute configuration of two bisabolol synthases in vitro was identified. Consistent with the literature report, Lippia dulcis α-bisabolol- 收稿日期:2019-04-16 基金项目:国家自然科学基金资助项目(31570305; 30900111) 第一作者:穆星(1994—),女,河北保定人,河北大学在读硕士研究生,主要从事分子细胞生物学研究. E-mail:mxmuxing@126.com 通信作者:李振秋(1974—),男,河北石家庄人,河北大学教授,主要从事分子细胞生物学研究. E-mail:cell@hbu.edu.cn第4期穆星等:青蒿α-bisabolol合酶产物绝对构型的确定synthase(LdBOS)catalyzes FPP in vitro, and the product configuration was(+)-epi-α-bisabolol; Artemisia annua α-bisabolol synthase(AaBOS)catalysed FPP in vitro, and the product was(+)-α-bisabolol. The catalytic product of AaBOS and LdBOS have the same carbon configuration at position 6, suggesting that the catalytic mechanism of these two enzymes is similar to that of the Artemisia annua amorpha-4,11-diene synthase(AaADS). The analysis of chiral compounds by gas and liquid chromatography has many advantages, such as high sensitivity, strong stability, easy to operate and so on. The separation and identification of the absolute configuration of chiral compounds α-bisabolol are of great significance in the fields of medicine, food and chemical industry.

Changes of antioxidant enzyme activity in callus of Hylotelephium tatarinowii under PEG 6 000 stress

HUANG Hangjun, LI Yi, MA Linxi, MA Weiwei, WANG Junli

2020, 40(4):  415-420.  DOI: 10.3969/j.issn.1000-1565.2020.04.012

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Callus of Hylotelephium tatarinowii(Maxim.)H. Ohba was treated by different concentrations of polyethylene glycol(PEG 6 000)for stress treatment in the study. The activity of peroxidase(POD), superoxide dismutase(SOD)and catalase(CAT)in callus was measured, and isozyme expressions of POD, SOD and esterase(EST)were analyzed by non-denatured polypropylene gel electrophoresis. The activity of POD, SOD and CAT increased gradually with the increase of concentration and the prolongation of stress time when the concentration of PEG 6 000 was 0.5%—5.0%, and the growth state of callus was normal. When the concentration of PEG 6 000 was 8%, the activity of POD, SOD and CAT decreased sharply with the prolongation of stress time.

InDel polymorphism of HMGA2 gene and its association with body size traits in Yunling cattle

LI Shipeng, WANG Fuwen, SHI Yingchao, XIANG Menmen, LEI Chuzhao, DANG Ruihua

2020, 40(4):  421-426.  DOI: 10.3969/j.issn.1000-1565.2020.04.013

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In order to explore the association between high mobility group AT-hook 2(HMGA2)gene polymorphism and body size traits in the Yunling cattle, 356 Yunling cattles were selected as subjects, and the genetic variation of HMGA2 gene was detected by polymerase chain reaction(PCR)amplification, polyacrylamide gel electrophoresis and sanger sequencing. The association between the genetic polymorphism and body size traits of the Yunling cattle was analyzed by SPSS software. The results showed that there was a 15 bp deletion at the site of ARS-UCD1.2 5:47872723 of HMGA2 gene, which was divided into three genotypes including II, ID and DD(I:insertion,D:deletion). There was significant difference in abdominal girth trait between these three genotypes, but there were no significant difference in other body size traits. Therefore, this indel polymorphism of HMGA2 gene can be used as the candidate molecular marker for abdominal girth trait selection in Yunling cattle.- DOI:10.3969/j.issn.1000-1565.2020.04.013云岭牛HMGA2基因InDel多态及其与生长性状的关联分析李世鹏,汪富文,史颖超,项门们,雷初朝,党瑞华(西北农林科技大学 动物科技学院,陕西 杨凌 712100)摘 要:为了探索高迁移率蛋白2(high mobility group AT-hook 2,HMGA2)基因插入缺失(InDel)多态与云岭牛体尺性状的关联性,以356头云岭牛为实验对象,采用多聚酶链式反应(polymerase chain reaction,PCR)扩增、聚丙烯酰胺凝胶电泳和Sanger测序的方法检测HMGA2基因的遗传变异,并利用SPSS软件对其InDel多态性与云岭牛体尺性状进行了关联分析.结果发现,在HMGA2基因的ARS-UCD1.2 5:47872723位点存在15 bp缺失,并根据缺失的类型,分为野生型(II)、杂合型(ID)、突变型(DD)(I:insertion,D:deletion),这3种不同的基因型所对应的个体,在腹围性状方面存在显著差异,而在其他的体尺性状上则不存在显著差异.因此,可以把HMGA2基因此位点InDel多态作为云岭牛腹围性状选择的候选分子标记,应用于肉牛新品种的标记辅助培育.关键词:云岭牛;HMGA2;多态性;体尺性状中图分类号:S813;S852.2 文献标志码:A 文章编号:1000-1565(2020)04-0421-06InDel polymorphism of HMGA2 gene and its associationwith body size traits in Yunling cattleLI Shipeng, WANG Fuwen, SHI Yingchao, XIANG Menmen, LEI Chuzhao, DANG Ruihua(College of Animal Science and Technology, Northwest Agriculture and Forestry University,Yangling 712100, China)Abstract: In order to explore the association between high mobility group AT-hook 2(HMGA2)gene polymorphism and body size traits in the Yunling cattle, 356 Yunling cattles were selected as subjects, and the genetic variation of HMGA2 gene was detected by polymerase chain reaction(PCR)amplification, polyacrylamide gel electrophoresis and sanger sequencing. The association between the genetic polymorphism and body size traits of the Yunling cattle was analyzed by SPSS software. The results showed that there was a 15 bp deletion at the site of ARS-UCD1.2 5:47872723 of HMGA2 gene, which was divided into three genotypes including II, ID and DD(I:insertion,D:deletion). There was significant difference in abdominal girth trait between these three genotypes, but there were no significant difference in other body size traits. Therefore, this indel polymorphism of HMGA2 gene can be used as the candidate molecular marker for abdominal girth trait selection in Yunling cattle.- 收稿日期:2019-06-10 基金项目:国家自然科学基金面上项目(81770514);杨凌示范区科技计划项目(2018NY-33);国家肉牛牦牛产业技术体系(CARS-37) 第一作者:李世鹏(1998—),男,河南濮阳人,西北农林科技大学在读硕士研究生.E-mail:964437867@qq.com 通信作者:党瑞华(1976—),男,陕西勉县人,西北农林科技大学副教授,博士生导师,主要从事家畜分子遗传研究.E-mail:dangruihua@nwsuaf.edu.cn第4期李世鹏等:云岭牛HMGA2基因InDel多态及其与生长性状的关联分析

Update strategy of SDN flow table based on time series and sets

LIU Zhenpeng, LI Ming, WANG Xinpeng, REN Shaosong, LI Xiaofei

2020, 40(4):  427-432.  DOI: 10.3969/j.issn.1000-1565.2020.04.014

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Flow table update is an issue that cannot be ignored in software-defined networks(SDN). Aiming at the problem of SDN flow table update consistency, a flow table update scheme based on timing and set is proposed. The classification of switches is separated by new and old paths, and the updating of the classification set is in accordance with a certain order. To ensure the transmission, the updating of the new flow table is realized before the deletion of the old one. Simulation experiments show that this scheme realized the consistency of the SDN flow table update under the same network speed. At the same time, this scheme showed the relatively short update time, the comparably low control load, and the reduction of the occupation space of switch flow table to ensure the better update effect.

A comparison on big data fuzzy K-means algorithm based on MapReduce and Spark

ZHAI Junhai, TIAN Shi, ZHANG Sufang, WANG Mohan, SONG Dandan

2020, 40(4):  433-440.  DOI: 10.3969/j.issn.1000-1565.2020.04.015

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The two big data fuzzy K-means algorithms based on Hadoop and Spark are compared in principle and in experiment, and the advantages and disadvantages of the two big data open source platforms are summarized. As the fuzzy K-means is an iterative algorithm, some data need to be iteratively handled to obtain the final clustering results. Accordingly, the two algorthms are compared on five aspects: running time, number of synchronization of tasks, number of files, fault tolerance, and resource consumption. Some valuable conclusions were obtained, which can be very helpful to reseachers in related fields, especifically for the ones engaging in the study of big data machine learning.

Optimization method of rural microgrid group and distribution network operation based on cooperative game

WANG Tao, HAN Jinglin, SHAO Hua, HE Chunguang, TAN Xiaolin, YAN Yongsheng

2020, 40(4):  441-448.  DOI: 10.3969/j.issn.1000-1565.2020.04.016

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For the problem of high penetration of new energy in rural areas, the method of operation optimization of rural microgrid group and distribution network based on cooperative game theory is proposed. In order to optimize the output level, battery charge and discharge, and interruptible load of each power supply in different periods of time in the microgrid, the minimum objective function of operation cost of microgrid including wind power, photovoltaic, biogas power generation, energy storage and interruptible load is established. With the minimum operating cost of each microgrid itself as the goal, this methad can consider the complementary and load characteristics of wind, light and biogas power generation within days, establish a cooperative network of microgrid groups based on cooperative game theory, make the residual power microgrid and the power-deficit microgrid realize the interoperability of power, reduce the overall operating cost of the alliance, increase the benefit of distribution of distribution of the alliance.The shapley value method is used to distribute the cost savings of the alliance. So as to realize the- DOI:10.3969/j.issn.1000-1565.2020.04.016基于合作博弈的农村微电网群与配电网运行优化方法王涛¹,韩璟琳¹,邵华¹,贺春光¹,檀晓林¹,闫永升²(1.国网河北省电力有限公司 经济技术研究院,河北 石家庄 050021;2.河北农业大学 机电工程学院,河北 保定 071000)摘 要:针对农村高渗透率新能源接纳问题,提出了基于合作博弈理论的农村微电网群与配电网运行优化方法.通过建立包含风电、光伏、沼气发电、储能和可中断负荷的微电网运行成本最小目标函数,优化微电网内不同时间段各电源的出力水平、蓄电池充放电和可中断的负荷.以各微电网自身运行成本最小为目标,考虑风、光、沼气发电日内的互补性和负荷特性,建立基于合作博弈论的微电网群合作联盟,使余电微电网与缺电微电网实现电能互用,降低联盟整体运行成本,增加配电网运营商收益.采用Shapley值法对联盟的节省成本进行分配,实现各微电网节省成本的公平分配.仿真算例证明了该方法的有效性.关键词:微电网;市场交易;合作博弈;Shapley值法中图分类号:TM73 文献标志码:A 文章编号:1000-1565(2020)04-0441-08Optimization method of rural microgrid group and distribution network operation based on cooperative gameWANG Tao¹, HAN Jinglin¹, SHAO Hua¹, HE Chunguang¹, TAN Xiaolin¹, YAN Yongsheng²(1. Institute of Economic and Technology, State Grid Hebei Electric Power Supply Co., Ltd., Shijiazhuang 050021,China; 2. College of Mechanical and Electrical Engineering, Hebei Agricultural University, Baoding 071000,China)Abstract: For the problem of high penetration of new energy in rural areas, the method of operation optimization of rural microgrid group and distribution network based on cooperative game theory is proposed. In order to optimize the output level, battery charge and discharge, and interruptible load of each power supply in different periods of time in the microgrid, the minimum objective function of operation cost of microgrid including wind power, photovoltaic, biogas power generation, energy storage and interruptible load is established. With the minimum operating cost of each microgrid itself as the goal, this methad can consider the complementary and load characteristics of wind, light and biogas power generation within days, establish a cooperative network of microgrid groups based on cooperative game theory, make the residual power microgrid and the power-deficit microgrid realize the interoperability of power, reduce the overall operating cost of the alliance, increase the benefit of distribution of distribution of the alliance.The shapley value method is used to distribute the cost savings of the alliance. So as to realize the- 收稿日期:2020-01-25 基金项目:国网河北省电力有限公司资助项目(SGHEJY00GHJS1900028);河北省重点研发计划项目(19226633D) 第一作者:王涛(1975—),男,河北石家庄人,国网河北省电力有限公司经济技术研究院总工程师,主要从事电网规划研究工作.E-mail:wangtao19750208@sina.com 通信作者:韩璟琳(1989—),男,河北衡水人,国网河北省电力有限公司经济技术研究院工程师,主要从事配电网规划研究.E-mail:jinglinoliver@163.com第4期王涛等:基于合作博弈的农村微电网群与配电网运行优化方法fair distribution of cost savings for each microgrid. The simulation study proves the effectiveness of this method.