Progress on extraction, isolation and antitumor activity of seaweed polypeptides

doi:10.3969/j.issn.1000-1565.2023.04.006

Abstract

Abstract: Seaweed is an important marine life and an important source of food for people. Extensive previous studies have confirmed that seaweed contains a lot of protein. However, due to the low content of polypeptides, polypeptides are difficult to be extracted and purified by the traditional methods. At present, enzymatic hydrolysis and chemical extraction are more commonly used for extraction, and chromatography and membrane separation are used for purification. A higher purity polypeptides could be obtained from seaweed, and its structure was identified using MS-related technique. Seaweed polypeptides have a strong effect against tumor, and the mechanisms are manifested in many aspects. In this paper, the latest extraction, purification and identification methods of seaweed polypeptides, as well as the latest research progress of their anti-tumor activity were reviewed, so as to provide reference for their further development and utilization.

Key words: seaweed polypeptide, extraction, purification, antitumor activity

CLC Number:

O241.6

SHEN Jinyang,ZHANG Xuan,YU Yuanyuan,ZHANG Shicheng,SHI Liuqing,DONG Zibo. Progress on extraction, isolation and antitumor activity of seaweed polypeptides[J]. Journal of Hebei University(Natural Science Edition), 2023, 43(4): 379-386.

References

[1] 刘媛. 海湾扇贝酶解物的制备、鉴定及其体内外抗肿瘤作用研究[D].保定: 河北农业大学,2015.
[2] 卢祺,包建强.国内外对海洋生物中抗肿瘤活性物质的研究[C] //2017年中国水产学会学术年会论文摘要集, 2017:233.
[3] 高玥,许倩楠,蔡明刚,等. 海洋来源药食同源品开发利用研究进展[J].中草药,2021,52(17):5455-5464. DOI:10.7501/j.issn.0253-2670.2021.17.037.
[4] 游伟.多肽及其衍生物的化学合成分析[J].农村经济与科技, 2017, 28(10): 273.
[5] VIJAYKRISHNARAJ M, PRABHASANKAR P. Marine protein hydrolysates: their present and future perspectives in food chemistry-a review[J]. RSC Adv, 2015, 5(44): 34864-34877. DOI: 10.1039/C4RA17205A.
[6] LEE J W, JEFFRIES T W. Efficiencies of acid catalysts in the hydrolysis of lignocellulosic biomass over a range of combined severity factors[J]. Bioresour Technol, 2011, 102(10): 5884-5890. DOI: 10.1016/j.biortech.2011.02.048.
[7] WANG W Q,LIU Z C, LIU Y J, et al. Plant polypeptides: A review on extraction, isolation, bioactivities and prospects[J]. INT J BIOL MACROMOL,2022,207:169-178. DOI: 10.1016/j.ijbiomac.2022.03.009.
[8] 林彬彬,唐旭,汪少芸.龙须菜蛋白的提取及其功能性质研究[J].中国食品学报, 2018, 18(12): 137-143. DOI: 10.16429/j.1009-7848.2018.12.017.
[9] 林红卫,覃海错,伍正清, 等.钝顶螺旋藻中藻蓝蛋白提取纯化新工艺[J].精细化工, 1998, 15(1): 18-20.
[10] FOSTER B J, FORTUNA M, MEDIA J, et al. Cryptophycin 1 cellular levels and effects in vitro using L1210 cells[J]. Invest New Drugs, 1998, 16(3): 199-204. DOI: 10.1023/a: 1006148127527.
[11] 林端权,郭泽镔,张怡,等.海洋生物活性肽的研究进展[J].食品工业科技, 2016, 37(18): 367-373. DOI: 10.13386/j.issn1002-0306.2016.18.062.
[12] 朱银玲,李思东,方富永.方格星虫多肽的酶解法制备工艺优化与抗氧化作用研究[J].化学通报, 2012, 75(7): 642-647.
[13] 林善婷,胡晓,李来好,等.水产蛋白源生物活性肽研究进展[J].大连海洋大学学报, 2020, 35(5): 775-785. DOI: 10.16535/j.cnki.dlhyxb.2019-266.
[14] 谢雪琼,钟婵,孙乐常,等.利用坛紫菜藻红蛋白制备脯氨酰内肽酶抑制肽[J].食品科学, 2019, 40(14): 123-129. DOI: 10.7506/spkx1002-6630-20180730-359.
[15] 谈森焱.小球藻酶解多肽的制备及其增强免疫力的功能研究[D].福州: 福建师范大学,2018.
[16] SHEIH I C, FANG T J, WU T K. Isolation and characterisation of a novel angiotensin I-converting enzyme(ACE)inhibitory peptide from the algae protein waste[J]. Food Chem, 2009, 115(1): 279-284. DOI: 10.1016/j.foodchem.2008.12.019.
[17] 蔡思学,李月,王芳,等.红毛藻R-藻红蛋白的提取纯化及在Cu²⁺检测中的应用[J].中国食品学报, 2022, 22(9): 276-284. DOI: 10.16429/j.1009-7848.2022.09.029.
[18] ZHENG Y, ZHANG Y, SAN S. Efficacy of a novel ACE-inhibitory peptide from sargassum maclurei in hypertension and reduction of intracellular endothelin-1[J]. Nutrients, 2020, 12(3): 653-668. DOI: 10.3390/nu12030653.
[19] XIANG H, SUN W, DONG X, et al. Fermentation-enabled wellness foods: A fresh perspective[J]. Food Science and Human Wellness,2019:203-243. DOI: 10.1016/j.fshw.2019.08.003.
[20] 张明洞.用螺旋藻制备蛋白激酶、活性肽及蛋白酶的方法: CN101265468A[P].2008-09-17.
[21] 付云,赵谋明,卢美杉,等.枯草芽孢杆菌YA215发酵螺旋藻渣产抑菌活性的工艺[J].食品与发酵工业, 2020, 46(4)146-152. DOI: 10.13995/j.cnki.11-1802/ts.022454.
[22] 余佳,赵岚,张瑞华,等.葛仙米藻胆蛋白的提取工艺优化及其纯化研究[J].食品研究与开发, 2018, 39(2):82-89. DOI: 10.3969/j.issn.1005-6521.2018.02.015.
[23] 孔祥佳,吴秋莹,谢勇,等.超声波协同酶解提取坛紫菜藻红蛋白工艺优化[J].食品与机械, 2017, 33(9):156-161. DOI: 10.13652/j.issn.1003-5788.2017.09.033.
[24] 蔡苗苗.舌状蜈蚣藻抗氧化肽的制备、纯化及结构鉴定[D].上海: 上海海洋大学,2020.
[25] 谢文熙,罗辉,黄永梅,等.紫球藻蛋白和活性肽的研究进展[J].食品工业, 2020, 41(9): 246-250. DOI: 10.3969/j.issn.2096-0387.2018.05.026.
[26] 赵冰冰,张发宇,陈裕,等.不同冻融条件对破壁提取巢湖水华新鲜蓝藻中藻蓝蛋白的影响[J].安徽农业科学, 2014, 42(17): 5345-5347. DOI: 10.3969/j.issn.0517-6611.2014.17.001.
[27] 徐远超.紫球藻培养及合成藻红蛋白工艺研究[D].济南: 齐鲁工业大学, 2019.
[28] 刘云姣.南极磷虾中含氟多肽的分离纯化及其结构研究[D].上海: 上海海洋大学,2019.
[29] 王竹君.螺旋藻抗肿瘤肽的分离及其活性研究[D].广州: 华南理工大学, 2015.
[30] 王文侠,翟丽萍,宋春丽.玉米浸泡液蛋白质的超滤分离研究[J].农产品加工(下), 2008(7): 115-117.
[31] 王晓琴.小球藻抗肿瘤多肽的分离及其微囊化研究[D].广州: 华南理工大学,2012.
[32] 白露.坛紫菜多肽的分离纯化及其抗肿瘤活性研究[D].广州: 华南理工大学, 2016.
[33] 陈丽莎.一种海藻活性肽的制备方法:CN107212422A[P].2017-06-23.
[34] STRANTMANN K, BURGOYNE D L, MOORE R E, et al. Hapalosin, a cyanobacterial cyclic depsipeptide with multidrug-resistance reversing activity[J]. Journal of Organic Chemistry, 1994,59(24):7 219-7226. DOI: 10.1021/jo00103a011.
[35] EDWARDS D J, MARQUEZ B L, NOGLE L M, et al. Structure and biosynthesis of the jamaicamides, new mixed polyketide-peptide neurotoxins from the marine cyanobacterium Lyngbya majuscula[J]. Chem Biol, 2004, 11(6): 817-833. DOI: 10.1016/j.chembiol.2004.03.030.
[36] 杨超凡.高纯度藻红蛋白的制备及其在染料敏化太阳能电池中的应用[D].哈尔滨: 哈尔滨工业大学,2021.
[37] GARCIA-VAQUERO M, MORA L, HAYES M. In vitro and in silico approaches to generating and identifying angiotensin-converting enzyme I inhibitory peptides from green macroalga ulva lactuca[J]. Mar Drugs, 2019, 17(4): 204.
[38] 朱林清,曾名湧.聚球藻PCC7002藻蓝蛋白提取纯化及热致褪色机理研究[J].食品工业科技, 2022, 43(16): 32-40. DOI: 10.13386/j.issn1002-0306.2021110096.
[39] 王天石,吴紫涵,张少斌,等.螺旋藻藻蓝蛋白的纯化及其水饮料稳定性[J].食品工业, 2021, 42(9): 134-138.
[40] 冯学珍.裙带菜ACE抑制肽的分离纯化及其抑制机理研究[D].南宁: 广西大学, 2021.
[41] JIAO K, GAO J, ZHOU T, et al. Isolation and purification of a novel antimicrobial peptide from Porphyra yezoensis[J]. J Food Biochem, 2019, 43(7): e12864. DOI: 10.1111/jfbc.12864.
[42] 郝可欣,胡文忠,侯梦阳,等.LC-MS/MS技术在食品质量与安全研究中的应用[C] //中国食品科学技术学会第十五届年会摘要集,2018:508.
[43] PÉREZ-MÍGUEZ R, PLAZA M, CASTRO-PUYANA M, et al. Separation and identification of peptides in hydrolysed protein extracts from edible macroalgae by HPLC-ESI-QTOF/MS[J]. Algal Res, 2019, 39: 101465.
[44] 李琦.海带降血糖多肽的分离合成及活性研究[D].大连: 大连理工大学,2021.
[45] 邓真真.两种红藻多肽的抗氧化及降血压活性研究[D].青岛: 中国科学院大学(中国科学院海洋研究所),2019.
[46] 褚宁宁, 陈雨豪, 陈娟娟, 等.基于nanoUPLC-MS/MS的螺旋藻游离肽组成分析[J].核农学报, 2020,34(4):735-744. DOI: 10.11869/j.issn.100-8551.2020.04.0735.
[47] 唐鹏杰,韩秋煜,包斌,等.海洋生物活性肽的制备、分离纯化及构效关系研究进展[J].食品工业科技, 2022, 43(24): 488-495.
[48] 李梅青.天然多肽在抗肿瘤药物研发中的应用及机制研究[D].北京: 中国科学院大学, 2021.
[49] 翟宇,王洪颖,刘旭,等.多肽抗肿瘤机制及应用的研究进展[J].肿瘤药学, 2021, 11(2): 133-139. DOI: 10.3969/j.issn.2095-1264.2021.02.02.
[50] ROSCA E V, KOSKIMAKI J E, RIVERA C G, et al. Anti-angiogenic peptides for cancer therapeutics[J]. Curr Pharm Biotechnol, 2011, 12(8): 1101-1116. DOI: 10.2174/138920111796117300.
[51] 冯钰瑶,米顺利.藻类活性肽的功能活性和应用研究进展[J].食品科技, 2022, 47(3): 259-264. DOI: 10.13995/j.cnki.11-1802/ts.024540.
[52] SAMTIVA M, ACHARYA S, PANDEY K K, et al. Production, purification, and potential health applications of edible seeds’ bioactive peptides: a concise review[J]. Foods,2021,10(11):2696. DOI: 10.3390/foods10112696.
[53] 白德发,廖小建,邱少玲,等.海藻中抗肿瘤活性环肽分离、结构修饰及活性[C] //全国第二届海洋与陆地多糖多肽及天然创新药物研发学术会议论文集,2015:220.
[54] 韩晶晶,董晓雷,杨方浩,等.C-藻蓝蛋白对宫颈癌HeLa细胞增殖与迁移的作用[J].青岛大学学报(医学版), 2022, 58(4): 494-499. DOI: 10.11712/jms.2096-5532.2022.58.069.
[55] 李芮,薛东华,商雪纯,等.解毒胶囊对乳腺增生大鼠PCNA和BcL-2表达影响[J].辽宁中医药大学学报, 2018, 20(5): 23-26. DOI: 10.13194/j.issn.1673-842x.2018.05.006.
[56] MAO H L, PANG Y X, ZHANG X L, et al. Smac peptide potentiates TRAIL- or paclitaxel-mediated ovarian cancer cell death in vitro and in vivo[J]. Oncol Rep, 2013, 29(2): 515-522. DOI: 10.3892/or.2012.2132.
[57] SIMMONS T L, NOGLE L M, MEDIA J, et al. Desmethoxymajusculamide C, a cyanobacterial depsipeptide with potent cytotoxicity in both cyclic and ring-opened forms[J]. J Nat Prod, 2009, 72(6): 1011-1016. DOI: 10.1021/np9001674.
[58] WRASIDLO W, MIELGO A, TORRES V A, et al. The marine lipopeptide somocystinamide A triggers apoptosis via caspase 8[J]. Proc Natl Acad Sci USA, 2008, 105(7): 2313-2318. DOI: 10.1073/pnas.0712198105.
[59] 张毅.肿瘤微环境免疫细胞与肿瘤患者预后相关分析[D].北京:北京协和医学院,2022.DOI:10.27648/d.cnki.gzxhu.2022.000092.
[60] 郝玮.藻蓝蛋白对荷瘤小鼠免疫功能及S₁₈₀肿瘤生长的影响[D].石家庄: 河北医科大学, 2005.
[61] 李冰,褚现明, 高美华.藻蓝蛋白-光动力疗法治疗小鼠HeLa细胞瘤的免疫和凋亡机制研究[J].中国激光医学杂志, 2011, 20(1): 1-6. DOI: 10.3872/j.issn.1007-385X.2017.02.005.
[62] 齐宏涛,刘颖,梁惠,等.重组藻红蛋白对H22荷瘤小鼠的调节与保护作用[J].中国新药杂志, 2020, 29(18):2129-2134. DOI: 10.3969/j.issn.1003-3734.2020.18.015.
[63] 韩敏敏,高程海,米顺利,等.藻蓝蛋白联合光动力疗法对SW1990胰腺癌细胞的抑制作用研究[J].广西中医药大学学报, 2022, 25(5): 10-15. DOI: 10.3969/j.issn.2095-4441.2022.05.004. (