Application of ceramic ultrafiltration membrane in the extraction of 5-hydroxy-L-tryptophan from fermentation broth

doi:10.3969/j.issn.1000-1565.2024.01.010

Abstract

Abstract: In order to improve the extraction and separation efficiency of 5-hydroxy-L-tryptophan(5-HTP)and reduce energy consumption and pollution emission, ceramic ultrafiltration membrane was selected to extract 5-HTP from fermentation broth. Firstly, the suitable membrane pore size of 10 nm was selected, and then the three optimum process parameters for the separation and extraction of 5-HTP by ceramic ultrafiltration membrane were further optimized: pH value of fermentation solution is 3.5, transmembrane pressure is 0.75 MPa, and transmembrane temperature is 35 ℃. Under this process condition, the penetration ratio of 5-HTP is more than 92%, and the rejection ratio of free protein is more than 90%, which meets the requirements of further concentration and crystallization to prepare 5-HTP, and achieves- DOI:10.3969/j.issn.1000-1565.2024.01.010陶瓷超滤膜在5-羟基-L-色氨酸发酵产物提取中的应用张怡君¹,田俊波^2,3,杨志彬^2,3,郝晋博¹,胡江林^2,3,吕志堂¹(1.河北大学生命科学学院,河北省微生物多样性研究与应用重点实验室,河北保定 0710022.河北维达康生物科技有限公司技术研发中心,河北保定 072152;3.保定保利瑞合生物科技有限公司技术研发中心,河北保定 072152)摘要:为提高5-羟基-L-色氨酸(5-hydroxy-L-tryptophan,5-HTP)提取分离效率,降低能耗和污染排放,选择陶瓷超滤膜应用于从发酵液中提取5-HTP的工艺,对陶瓷超滤膜的过滤条件进行优化.首先优选出适合的膜孔径为10 nm,进一步优化得到陶瓷超滤膜分离提取5-HTP的3个最优工艺参数:发酵液pH值3.5,跨膜压差0.75 MPa,料液过膜温度35 ℃.在此工艺条件下,5-HTP的透过率在92%以上,对游离蛋白质截留率达 90%以上,满足进一步浓缩、结晶制备5-HTP的要求,达到了优化预期效果,为陶瓷超滤膜应用于从发酵液中工业化提取5-HTP提供了可靠依据.关键词:陶瓷超滤膜;5-羟基-L-色氨酸;过滤条件;透过率;截留率中图分类号:Q81 文献标志码:A 文章编号:1000-1565(2024)01-0077-07Application of ceramic ultrafiltration membrane in the extraction of 5-hydroxy-L-tryptophan from fermentation brothZHANG Yijun¹, TIAN Junbo^2,3, YANG Zhibin^2,3, HAO Jinbo¹, HU Jianglin^2,3, LYU Zhitang¹(1. Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University,Baoding 071002, China;2. Technology Research and Development Center,Hebei VDK Natura Syn Co. Ltd., Baoding 072152, China;3. Technology Research and Development Center,Baoding BLRH Biotech Co. Ltd., Baoding 072152, China)Abstract: In order to improve the extraction and separation efficiency of 5-hydroxy-L-tryptophan(5-HTP)and reduce energy consumption and pollution emission, ceramic ultrafiltration membrane was selected to extract 5-HTP from fermentation broth. Firstly, the suitable membrane pore size of 10 nm was selected, and then the three optimum process parameters for the separation and extraction of 5-HTP by ceramic ultrafiltration membrane were further optimized: pH value of fermentation solution is 3.5, transmembrane pressure is 0.75 MPa, and transmembrane temperature is 35 ℃. Under this process condition, the penetration ratio of 5-HTP is more than 92%, and the rejection ratio of free protein is more than 90%, which meets the requirements of further concentration and crystallization to prepare 5-HTP, and achieves- 收稿日期:2023-08-28;修回日期:2023-09-06 基金项目:河北省重点研发计划项目(22372403D) 第一作者:张怡君(1996—),女,河北大学在读硕士研究生,主要从事微生物资源与系统学研究.E-mail: zyjdyx898@163.com 通信作者:吕志堂(1973—),男,河北大学教授,博士,主要从事微生物资源与系统学、微生物组学和合成生物学研究.E-mail: lzt325@hbu.edu.cn胡江林(1970—),男,研究员,主要从事制药工程、合成生物学研究.E-mail:hujianglin@vdkbio.com第1期张怡君等:陶瓷超滤膜在5-羟基-L-色氨酸发酵产物提取中的应用the expected effect of the optimization. It provides a reliable basis for the application of ceramic ultrafiltration membrane to industrial extraction of 5-HTP from fermentation broth.

Key words: ceramic ultrafiltration membrane, 5-hydroxy-L-tryptophan, filtration condition, permeate ratio, rejection ratio

CLC Number:

ZHANG Yijun, TIAN Junbo, YANG Zhibin, HAO Jinbo, HU Jianglin, LYU Zhitang. Application of ceramic ultrafiltration membrane in the extraction of 5-hydroxy-L-tryptophan from fermentation broth[J]. Journal of Hebei University(Natural Science Edition), 2024, 44(1): 77-83.

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