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Application of ceramic ultrafiltration membrane in the extraction of 5-hydroxy-L-tryptophan from fermentation broth
- ZHANG Yijun, TIAN Junbo, YANG Zhibin, HAO Jinbo, HU Jianglin, LYU Zhitang
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2024, 44(1):
77-83.
DOI: 10.3969/j.issn.1000-1565.2024.01.010
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In order to improve the extraction and separation efficiency of 5-hydroxy-L-tryptophan(5-HTP)and reduce energy consumption and pollution emission, ceramic ultrafiltration membrane was selected to extract 5-HTP from fermentation broth. Firstly, the suitable membrane pore size of 10 nm was selected, and then the three optimum process parameters for the separation and extraction of 5-HTP by ceramic ultrafiltration membrane were further optimized: pH value of fermentation solution is 3.5, transmembrane pressure is 0.75 MPa, and transmembrane temperature is 35 ℃. Under this process condition, the penetration ratio of 5-HTP is more than 92%, and the rejection ratio of free protein is more than 90%, which meets the requirements of further concentration and crystallization to prepare 5-HTP, and achieves- DOI:10.3969/j.issn.1000-1565.2024.01.010陶瓷超滤膜在5-羟基-L-色氨酸发酵产物提取中的应用张怡君1,田俊波2,3,杨志彬2,3,郝晋博1,胡江林2,3,吕志堂1(1.河北大学 生命科学学院,河北省微生物多样性研究与应用重点实验室,河北 保定 0710022.河北维达康生物科技有限公司 技术研发中心,河北 保定 072152;3.保定保利瑞合生物科技有限公司 技术研发中心,河北 保定 072152)摘 要:为提高5-羟基-L-色氨酸(5-hydroxy-L-tryptophan,5-HTP)提取分离效率,降低能耗和污染排放,选择陶瓷超滤膜应用于从发酵液中提取5-HTP的工艺,对陶瓷超滤膜的过滤条件进行优化.首先优选出适合的膜孔径为10 nm,进一步优化得到陶瓷超滤膜分离提取5-HTP的3个最优工艺参数:发酵液pH值3.5,跨膜压差0.75 MPa,料液过膜温度35 ℃.在此工艺条件下,5-HTP的透过率在92%以上,对游离蛋白质截留率达 90%以上,满足进一步浓缩、结晶制备5-HTP的要求,达到了优化预期效果,为陶瓷超滤膜应用于从发酵液中工业化提取5-HTP提供了可靠依据.关键词:陶瓷超滤膜;5-羟基-L-色氨酸;过滤条件;透过率;截留率中图分类号:Q81 文献标志码:A 文章编号:1000-1565(2024)01-0077-07Application of ceramic ultrafiltration membrane in the extraction of 5-hydroxy-L-tryptophan from fermentation brothZHANG Yijun1, TIAN Junbo2,3, YANG Zhibin2,3, HAO Jinbo1, HU Jianglin2,3, LYU Zhitang1(1. Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life Sciences, Hebei University,Baoding 071002, China;2. Technology Research and Development Center,Hebei VDK Natura Syn Co. Ltd., Baoding 072152, China;3. Technology Research and Development Center,Baoding BLRH Biotech Co. Ltd., Baoding 072152, China)Abstract: In order to improve the extraction and separation efficiency of 5-hydroxy-L-tryptophan(5-HTP)and reduce energy consumption and pollution emission, ceramic ultrafiltration membrane was selected to extract 5-HTP from fermentation broth. Firstly, the suitable membrane pore size of 10 nm was selected, and then the three optimum process parameters for the separation and extraction of 5-HTP by ceramic ultrafiltration membrane were further optimized: pH value of fermentation solution is 3.5, transmembrane pressure is 0.75 MPa, and transmembrane temperature is 35 ℃. Under this process condition, the penetration ratio of 5-HTP is more than 92%, and the rejection ratio of free protein is more than 90%, which meets the requirements of further concentration and crystallization to prepare 5-HTP, and achieves- 收稿日期:2023-08-28;修回日期:2023-09-06 基金项目:河北省重点研发计划项目(22372403D) 第一作者:张怡君(1996—),女,河北大学在读硕士研究生,主要从事微生物资源与系统学研究.E-mail: zyjdyx898@163.com 通信作者:吕志堂(1973—),男,河北大学教授,博士,主要从事微生物资源与系统学、微生物组学和合成生物学研究.E-mail: lzt325@hbu.edu.cn胡江林(1970—),男,研究员,主要从事制药工程、合成生物学研究.E-mail:hujianglin@vdkbio.com第1期张怡君等:陶瓷超滤膜在5-羟基-L-色氨酸发酵产物提取中的应用the expected effect of the optimization. It provides a reliable basis for the application of ceramic ultrafiltration membrane to industrial extraction of 5-HTP from fermentation broth.