Abstract: To establish an HPLC method for the determination of thiolidazine hydrochloride in chicken,the best detection wavelength of thiolidazine hydrochloride was determined by high performance liquid chromatograph(HPLC)with a diode array detector.The samples were extracted with ethyl acetate as solvent, and the fat was removed with n-hexane, concentrated by nitrogen blowing, then dissolved with acetonitrile, purified with 60 mg HLB solid phase extraction column, and finally filtered with 0.22 μm organic membrane. The liquid chromatographic conditions were as follows: mobile phase consisted of acetonitrile-0.1% formic acid aqueous solution(V∶V=35∶65), the flow rate was 1 mL/min, and diode array detector was used for detection. Quantitative analysis was carried out by the external standard method. The results show that the linearity of the method is good in the range of 0.1—10.0 μg/mL. The limit of detection was 1.2 μg/kg, the limit of quantitation was 4.0 μg/kg, and the recoveries were 8.96%—98.58%. The HPLC method established in this study for the determination of thiolidazine hydrochloride in chicken has the advantages of good precision, convenient operation and high recovery.It can provide a technical method for the detection of tranquilizers in animal food.

Key words: thiolidazine hydrochloride, chicken, high performance liquid chromatography, external standard method