Journal of Hebei University (Natural Science Edition) ›› 2018, Vol. 38 ›› Issue (4): 392-395.DOI: 10.3969/j.issn.1000-1565.2018.04.008

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A method of RNA extraction from pear tree tissue

SHEN Shigang1, GAO Yanxia1,2,WANG Fengshan2,ZHOU Yuyan2,XIE Ying2, FENG Li2,HAO Guangfei3   

  1. 1.College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; 2.Hebei Provincial Institute for Drug Control, Shijiazhuang 050011, China; 3.College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, China
  • Received:2018-01-03 Online:2018-07-25 Published:2018-07-25

Abstract: A method of RNA extraction is designed to solve the key problems of low yield, degradation and pollution in the pear tree RNA extraction process. Total RNA is obtained intacted with high purity and content by using this method, and be improved the data quality of the second generation sequencing is improved.

Key words: pear tree tissue, RNA, extraction method

CLC Number: